Article
Biochemistry & Molecular Biology
Michaela Nicole Hoehne, Lianne J. H. C. Jacobs, Kim Jasmin Lapacz, Gaetano Calabrese, Lena Maria Murschall, Teresa Marker, Harshita Kaul, Aleksandra Trifunovic, Bruce Morgan, Mark Fricker, Vsevolod V. Belousov, Jan Riemer
Summary: Hydrogen peroxide (H2O2) plays a key role in signaling at physiological levels, but can cause molecular damage at high concentrations. This study used a genetically encoded H2O2 sensor to investigate how mitochondria release H2O2 in intact cells. The researchers found that H2O2 is released by mitochondria directly at the surface of the organelle and in the bulk cytosol, and that gradient formation is controlled by cytosolic peroxiredoxins.
Article
Cell Biology
Bumsoo Ahn, Rojina Ranjit, Parker Kneis, Hongyang Xu, Katarzyna M. Piekarz, Willard M. Freeman, Michael Kinter, Arlan Richardson, Qitao Ran, Susan V. Brooks, Holly Van Remmen
Summary: The study aimed to determine the impact of mitochondrial hydrogen peroxide on muscle aging and contractile dysfunction. Results showed that muscle-specific overexpression of mPRDX3 can reduce mitochondrial H2O2 generation, improve mitochondrial function, and mitigate loss of muscle quantity and quality, despite the persistence of neuromuscular junction impairment.
Review
Chemistry, Multidisciplinary
Junling Yin, Jingting Zhan, Qingxia Hu, Shuhong Huang, Weiying Lin
Summary: Ferroptosis is a regulatory cell death process that is distinct from caspase-dependent apoptosis and plays a crucial role in living organisms. Understanding the level fluctuation of key target analytes during ferroptosis is significant for disease treatment and drug design. Multiple organic fluorescent probes have been developed to monitor various bio-related molecules and microenvironments during ferroptosis, and this review aims to highlight the latest breakthrough results in this field.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Wuyang Lv, Lei Liang, Dongyang Liu, Cuicui Li, Liao Jia, Yingyu Jin
Summary: This study investigated the role of AQP1 in ferroptosis, macrophage polarization, mitochondrial dysfunction, and impaired autophagy of LPS-stimulated RAW264.7 cells. The results showed that AQP1 promotes ferroptosis, M1 polarization, mitochondrial dysfunction, and autophagy impairment by inhibiting the expression of P53 in LPS-stimulated RAW264.7 cells. AQP1 or P53 may be considered as crucial determiners that regulate the biological behavior of RAW264.7 cells stimulated by LPS.
DNA AND CELL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Lianne J. H. C. Jacobs, Jan Riemer
Summary: Compartmentalisation of eukaryotic cells enables the coexistence of different cellular processes. Small redox molecules, in cooperation with proteins, establish specific subcellular redox compartments that support oxidative protein folding and redox signaling. Dysregulated redox homeostasis is directly linked to various diseases. This review focuses on the mechanisms regulating redox homeostasis in the mitochondrial subcompartments of mammalian cells.
Article
Biochemistry & Molecular Biology
Caitlin E. Miller, Fangfang Xu, Yanming Zhao, Wei Luo, Weixiong Zhong, Kristy Meyer, Rani Jayswal, Heidi L. Weiss, William H. St Clair, Daret K. St Clair, Luksana Chaiswing
Summary: This study reveals a novel adaptive response to radiation treatment that promotes cancer recurrence. It shows that hydrogen peroxide (H2O2) can reprogram mitochondrial homeostasis in prostate cancer cells, allowing them to survive and regrow after radiation treatment. The study also demonstrates that radiation induces the release of extracellular vesicles (EVs) carrying mitochondrial antioxidant proteins, and H2O2 enhances the production of these EVs. Furthermore, functional mitochondria enhance cancer cell survival after radiation treatment.
Article
Engineering, Environmental
Yuting Wang, Huming Yan, Yongkang Yue, Yongbin Zhang, Fangjun Huo, Fangqin Cheng, Caixia Yin
Summary: Ferroptosis, a type of cell death caused by lipid peroxidation, is closely related to the development of tumors, ischemia-reperfusion injury, and other diseases. It involves the accumulation of reactive oxygen species and thiols, which promote or inhibit ferroptosis. Understanding the dynamic correlation between reactive oxygen species and active sulfur in these pathways is crucial for revealing the regulation mechanism of ferroptosis.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Shan Lu, Xuan-zhong Wang, Chuan He, Lei Wang, Shi-peng Liang, Chong-cheng Wang, Chen Li, Tian-fei Luo, Chun-sheng Feng, Zhen-chuan Wang, Guang-fan Chi, Peng-fei Ge
Summary: The study demonstrates that brucine inhibits glioma cell growth and induces ferroptosis by promoting the accumulation of H2O2 and iron via activation of ATF3.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Engineering, Environmental
Jia Wang, Xintao Ding, Xinquan Zhou, Zhuwei Liao, Jiayi Cai, Siqi Wang, Ali Jawad, Jerosha Ifthikar, Lie Yang, Songlin Wang, Zhuqi Chen
Summary: Careful remediation of organoarsenics in soil is urgently desired but sadly lacks attention. Here, we report a versatile Fe2+/Urea Hydrogen Peroxide (UHP) process for organoarsenic remediation and nitrogen supplement in soil. Mechanism insight was studied in water, demonstrating efficient degradation and satisfying mineralization performance of ROX. The remediation was further achieved in soil with satisfying removal of ROX and immobilization of total arsenic, and the injection of Fe2+/UHP resulted in significantly higher concentration of total nitrogen and promoted growth of lettuce.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Plant Sciences
David Israel, Seong Hee Lee, Thomas Matthew Robson, Janusz Jerzy Zwiazek
Summary: This study demonstrates the physiological relevance of plasma membrane aquaporins (PIPs) in the diffusion of H2O2 in Arabidopsis thaliana roots. Specific PIPs, such as PIP2;3, PIP1;1, and PIP2;6, have been identified to facilitate H2O2 diffusion and play a role in regulating stress responses.
Article
Biochemistry & Molecular Biology
Anja V. Gruszczyk, Alva M. Casey, Andrew M. James, Hiran A. Prag, Nils Burger, Georgina R. Bates, Andrew R. Hall, Fay M. Allen, Thomas Krieg, Kourosh Saeb-Parsy, Michael P. Murphy
Summary: Cell models of cardiac ischemia-reperfusion injury are crucial for studying cardiac pathology, however, current monolayer cell models are inadequate in replicating in vivo injury. This study identified two reasons for the limitations: the presence of oxygen in cellular hypoxia models sustains mitochondrial oxidative phosphorylation, and the loss of lactate from cells during ischemia sustains glycolysis. By incubating isolated adult mouse cardiomyocytes anoxically and inhibiting lactate efflux, key markers of in vivo ischemia were recapitulated, allowing for the assessment of important aspects of cardiac IR injury in vitro.
Review
Cell Biology
Ryan J. J. Mailloux, Cathryn Grayson, Olivia Koufos
Summary: Protein S-glutathionylation has been proposed as a regulator of cell metabolism for four decades. This redox-sensitive covalent modification serves as a cell-wide signaling platform and plays a key role in embryonic development and regulation of physiological functions. It functions as a feedback loop for inhibiting mitochondrial hydrogen peroxide (H2O2) production and desensitizing mitochondrial redox signals.
Article
Chemistry, Multidisciplinary
Rongrong Zhou, Qiyao Peng, Dan Wan, Chao Yu, Yuan Zhang, Yi Hou, Quan Luo, Xiong Li, Shuihan Zhang, Lin Xie, Pinghua Ou, Yongbo Peng
Summary: A new lysosome-targeting fluorescent probe NPT-H2O2 was developed for ratiometric detection and imaging of H2O2, especially in lysosomes and inflammation models. The probe exhibited rapid response, high selectivity and sensitivity towards H2O2, along with low cytotoxicity. The results suggest that this probe can serve as a powerful tool to investigate the physiological and pathological relationship between inflammation and lysosomal H2O2.
Article
Spectroscopy
Han-Chen Zhang, Di-Hua Tian, Ya-Long Zheng, Fang Dai, Bo Zhou
Summary: Hydrogen peroxide plays a crucial role in biological processes, and monitoring its levels is important for exploring physiological and pathological roles. The newly developed probe BTFMB has excellent detection performance and can successfully monitor the generation of H2O2 and its accumulation during ferroptosis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Multidisciplinary
Jing Su, Shuping Zhang, Cairong Wang, Min Li, Jiajia Wang, Feng Su, Zhijun Wang
Summary: The fluorescent probe GW-1 designed and synthesized for monitoring hydrogen peroxide utilizes a dual-locked model system to activate its fluorescence, avoiding alkalizing effects and exhibiting high selectivity over other ROS. These features make it ideal for sensing H2O2 and pH changes in bioanalytical and biomedical applications.
Article
Biochemistry & Molecular Biology
Kazuo Tomita, Sayuri Yamanishi-Taira, Kento Igarashi, Yuichi Oogai, Yoshikazu Kuwahara, Mehryar Habibi Roudkenar, Amaneh Mohammadi Roushandeh, Shouichi Miyawaki, Akihiro Kurimasa, Tomoaki Sato
Summary: Inflammation, especially neuroinflammation caused by stress, can lead to dysfunction in the central nervous system (CNS). This study investigated the impact of lipopolysaccharides (LPSs) on the CNS. The findings suggest that LPSs derived from oral bacteria can reduce the expression of KCC2, a Cl- transporter, leading to CNS dysfunction. The study also revealed the involvement of TLR4, IL-1 beta, and REST gene expressions in the inactivation of KCC2. Further experiments showed that LPS treatment changed the GABA function from inhibitory to excitatory. Oxytocin was found to rescue the reduction in KCC2 expression caused by LPSs, while vasopressin could not. These findings highlight the potential role of LPSs from oral bacteria in increasing the risk for brain disorders and suggest oxytocin as a potential candidate for treating abnormal behaviors associated with psychiatric disorders.
Review
Cell Biology
Mehdi Rabiee Valashedi, Chia Bamshad, Nima Najafi-Ghalehlou, Amirsadegh Nikoo, Kazuo Tomita, Yoshikazu Kuwahara, Tomoaki Sato, Amaneh Mohammadi Roushandeh, Mehryar Habibi Roudkenar
Summary: Cancer cells' resistance to ferroptosis, a new form of regulated cell death, is a major challenge in cancer therapy. Non-coding RNAs have been found to play a role in regulating ferroptosis, and targeting these RNAs could potentially overcome cancer drug resistance. Gene therapy, including gene-editing technology, may aid in developing ncRNA-based ferroptosis targeting as a novel therapeutic strategy for cancer treatment.
Article
Biochemistry & Molecular Biology
Kento Igarashi, Haruki Iwai, Koh-ichi Tanaka, Yoshikazu Kuwahara, Junichi Kitanaka, Nobue Kitanaka, Akihiro Kurimasa, Kazuo Tomita, Tomoaki Sato
Summary: This study found that nasal oxytocin administration after cranial irradiation can protect the neurological function of mice, alleviate cognitive dysfunction, and exert multifaceted neuroprotective effects on DNA damage, while maintaining the chloride ion concentration in neuronal cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Otorhinolaryngology
Saghi Jani Kargar Moghaddam, Amaneh Mohammadi Roushandeh, Mehryar Habibi Roudkenar, Shadman Nemati, Nima Najafi-Ghalehlou, Toofan Pakzad, Masoud Hamidi
Summary: This study aimed to investigate the clinical significance of vascular endothelial growth factor (VEGF), latent membrane protein 1 (LMP1), and tumor necrosis factor-alpha (TNF-alpha) as biomarkers for nasopharyngeal carcinoma (NPC) and evaluate their correlations in a specific region of Iran.
INTERNATIONAL ARCHIVES OF OTORHINOLARYNGOLOGY
(2023)
Article
Medicine, Research & Experimental
Mehdi Rabiee Valashedi, Amaneh Mohammadi Roushandeh, Kazuo Tomita, Yoshikazu Kuwahara, Zahra Pourmohammadi-Bejarpasi, Pouya Safarzadeh Kozani, Tomoaki Sato, Mehryar Habibi Roudkenar
Summary: Targeting Lcn2 using CRISPR/Cas9 can inhibit cell proliferation and migration capability in breast cancer cells, enhance sensitivity to cisplatin, and promote ferroptotic cell death.
Review
Cell Biology
Chia Bamshad, Nima Najafi-Ghalehlou, Zahra Pourmohammadi-Bejarpasi, Kazuo Tomita, Yoshikazu Kuwahara, Tomoaki Sato, Alireza Feizkhah, Amaneh Mohammadi Roushnadeh, Mehryar Habibi Roudkenar
Summary: Mitochondria play a crucial role in ageing and neurodegenerative disorders. This review examines the functions of mitochondria and the pathways contributing to mitochondrial dysfunction. It also discusses the therapeutic effects of mitophagy and highlights the experiments involving mitochondrial transfer in animal models of neurodegenerative disorders.
Review
Cell Biology
Yoshikazu Kuwahara, Kazuo Tomita, Mehryar Habibi Roudkenar, Amaneh Mohammadi Roushandeh, Tomoaki Sato, Akihiro Kurimasa
Summary: In order to understand the molecular mechanisms responsible for radioresistance in cancer cells, clinically relevant radioresistant (CRR) cell lines were established from various human cancer cell lines. These CRR cells demonstrated the ability to proliferate even under exposure to standard tumor radiotherapy doses. However, it was found that this radiation-induced radioresistance is reversible, as CRR cells that did not receive maintenance irradiation lost their radioresistance. Comparing CRR and CRR-NoIR cells is considered the best approach to identify factors involved in tumor radioresistance.
Article
Cell Biology
Kazuki Hasegawa, Ryo Saga, Kentaro Ohuchi, Yoshikazu Kuwahara, Kazuo Tomita, Kazuhiko Okumura, Tomoaki Sato, Manabu Fukumoto, Eichi Tsuruga, Yoichiro Hosokawa
Summary: 4-MU enhances radiosensitization of radioresistant cells by increasing intracellular oxidative stress and suppressing cancer stem cell-like phenotype. These results may be associated with HAS3 or intracellular HA synthesized by HAS3.
Review
Oncology
Hamed Hosseinalizadeh, Mehryar Habibi Roudkenar, Amaneh Mohammadi Roushandeh, Yoshikazu Kuwahara, Kazuo Tomita, Tomoaki Sato
Summary: This article reviews the application of NK cell-based immunotherapy in GBM patients, with a focus on the importance of genetically engineered NK cells and key receptors in treatment.
Article
Immunology
Chia Bamshad, Mehryar Habibi Roudkenar, Mahmoud Abedinzade, Shahrokh Yousefzadeh Chabok, Zahra Pourmohammadi-Bejarpasi, Nima Najafi-Ghalehlou, Tomoaki Sato, Kazuo Tomita, Ali Jahanian-Najafabadi, Alireza Feizkhah, Amaneh Mohammadi Roushandeh
Summary: Traumatic brain injury (TBI) has high mortality rates worldwide, and survivors often have long-term disabilities. Current TBI treatment options are inefficient, emphasizing the need for repairing damaged brain tissue at the cellular and molecular levels. Mitochondrial transplantation has emerged as a promising treatment approach, as it supplies healthy and functional mitochondria to damaged tissue. In this study, researchers successfully transplanted mitochondria derived from human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) into rat models of TBI, leading to improved motor function and reduced cell apoptosis and brain inflammation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Cell Biology
Kazuo Tomita, Hiroko P. Indo, Tomoaki Sato, Jitbanjong Tangpong, Hideyuki J. Majima
Summary: We developed a method to examine the mutation frequencies of the A3243G mutation of mitochondrial DNA (mtDNA) in patients with MELAS syndrome. This method utilizes qPCR assay with TaqMan probes to detect the mutated and wild-type sequences of mtDNA. The results were used to generate a standard curve of mutation frequency, which can be used for mutation frequency analysis in various diseases. The method showed a low standard deviation and can be applied to other genetic studies.
Article
Biochemistry & Molecular Biology
Junichi Kitanaka, Nobue Kitanaka, Kazuo Tomita, F. Scott Hall, Kento Igarashi, George R. R. Uhl, Tomoaki Sato
Summary: We used a newly modified, infrared beam sensor-based automated apparatus to investigate the effects of glycogen synthase kinase-3 (GSK-3) inhibitors on morphine-induced Straub's tail reaction (STR) in mice. We found that pretreatment with the GSK-3 inhibitors significantly inhibited STR and reduced its duration in a dose-dependent manner. The inhibitory effect of the inhibitors on STR might not depend on the direct blockade of GSK-3 beta function. Additionally, the mechanism of action of the inhibitors seems to be central rather than peripheral.
NEUROCHEMICAL RESEARCH
(2023)
Article
Cell Biology
Amirsadegh Nikoo, Mehryar Habibi Roudkenar, Tomoaki Sato, Yoshikazu Kuwahara, Kazuo Tomita, Zahra Pourmohammadi-Bejarpasi, Nima Najafi-Ghalehlou, Amaneh Mohammadi Roushandeh
Summary: Despite recent advancements, cancer remains a leading cause of death worldwide, and mitochondrial dysfunction is associated with cancer initiation, progression, chemotherapy resistance, and ferroptotic cell death failure. The significance of mitochondria in different cancer types is still debated. This study aimed to determine the outcome of transferring healthy mitochondria into aggressive prostate cancer cells and evaluate combination therapy with or without the ferroptosis inducer erastin, potentially providing a new therapeutic strategy for various cancers.
Article
Oncology
Sahar Evazi Bakhshi, Amaneh Mohammadi Roushandeh, Mehryar Habibi Roudkenar, Shima Shekarchi, Mohammad Hadi Bahadori
Summary: In this study, the CRISPR/Cas9 technology was used to knock out the HO-1 gene in breast cancer cells, and its effects on cell proliferation, cisplatin sensitivity, apoptosis, and migration capability were studied. The results revealed that suppression of HO-1 significantly inhibited cell proliferation, increased sensitivity to cisplatin-induced toxicity and apoptosis, and abolished cell migration capability.
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
