Journal
DEVELOPMENTAL CELL
Volume 37, Issue 1, Pages 98-104Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2016.03.010
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Funding
- Peter und Traudl Engelhorn program
- Marie-Curie FP7 [FP7-PEOPLE-2013-IEF]
- Novartis Research Foundation
- Swiss National Science Foundation [31003A_125386, NRP 63]
- MetastasiX project from the Swiss Initiative in Systems Biology (SystemsX.ch)
- Swiss National Science Foundation (SNF) [31003A_125386] Funding Source: Swiss National Science Foundation (SNF)
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Samans et al. (2014) reported the enrichment of nucleosomes in human and bovine spermatozoa at centromere repeats and retrotransposon sequences such as LINE-1 and SINE. We demonstrate here that nucleosomal enrichments at repetitive sequences as reported result from bioinformatic analyses that make redundant use of sequencing reads that map to multiple locations in the genome. To illustrate that this computational approach is flawed, we observed comparable artificial enrichments at repetitive sequences when aligning control genomic DNA or simulated reads of uniform genome coverage. These results imply that the main conclusions of the article by Samans et al. (2014) are confounded by an inappropriate computational methodology used to analyze the primary data.
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