Article
Multidisciplinary Sciences
Federico Gulluni, Lorenzo Prever, Huayi Li, Petra Krafcikova, Ilaria Corrado, Wen-Ting Lo, Jean Piero Margaria, Anlu Chen, Maria Chiara De Santis, Sophie J. Cnudde, Joseph Fogerty, Alex Yuan, Alberto Massarotti, Nasrin Torabi Sarijalo, Oscar Vadas, Roger L. Williams, Marcus Thelen, David R. Powell, Markus Schueler, Michael S. Wiesener, Tamas Balla, Hagit N. Baris, Dov Tiosano, Brian M. McDermott, Brian D. Perkins, Alessandra Ghigo, Miriam Martini, Volker Haucke, Evzen Boura, Giorgio Roberto Merlo, David A. Buchner, Emilio Hirsch
Summary: The cytokinetic process in the lens, which depends on PI3K-C2alpha, PI(3,4)P-2, and VPS36, is essential for preventing premature senescence and cataract. Loss of these components impairs cytokinesis and leads to cell damage, triggering senescence in the lens of fish, mice, and humans.
Review
Cell Biology
Virginia Andrade, Arnaud Echard
Summary: This paper reviews the mechanical context of cytokinetic abscission and summarizes the mechanisms of intercellular bridge tension release and abscission trigger. Additionally, it discusses whether traction-induced mechanical cell rupture could be an alternative mechanism of abscission and suggests future research directions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sascha Pust, Andreas Brech, Catherine Sem Wegner, Harald Stenmark, Kaisa Haglund
Summary: Cellular abscission is the final step of cytokinesis. ALIX and TSG101 proteins are involved in recruiting ESCRT-III to the midbody, and together with CHMP4B, they form spiral-like structures from the midbody to the abscission site. ALIX and CHMP4B are transported in vesicles along microtubules by the kinesin-1 motor protein to the cytokinetic bridge and midbody, contributing to abscission.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biology
Dawn M. Wenzel, Douglas R. Mackay, Jack J. Skalicky, Elliott L. Paine, Matthew S. Miller, Katharine S. Ullman, Wesley Sundquist, Aurelien Roux
Summary: In this study, the interactions between human MIT domains and ESCRT-III tails were comprehensively mapped and quantified. Several new interactions were discovered, and the structure of the SPASTIN MIT domain in complex with the IST1 C-terminal tail was reported. The study also revealed the localization and functions of three MIT enzymes in cytokinetic abscission.
Article
Virology
Kosuke Oda, Yasuyuki Matoba, Masanori Sugiyama, Takemasa Sakaguchi
Summary: The study reveals the crucial role of the binding between the Respirovirus C protein and Alix in viral replication, with mutant C proteins of varying binding affinities showing impaired virus production. This interaction is essential for viral budding and provides insights for the development of new antiviral drugs against hPIV1.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Adrien Presle, Stephane Fremont, Audrey Salles, Pierre-Henri Commere, Nathalie Sassoon, Clarisse Berlioz-Torrent, Neetu Gupta-Rossi, Arnaud Echard
Summary: The midbody remnant (MBR) generated during cell division contains information that induces cancer cell proliferation, influences cell polarity, and promotes dorso-ventral axis specification. However, the mechanisms by which MBRs are captured by daughter cells or distant cells are poorly understood. A study found that BST2/tetherin, a restriction factor known for blocking the release of enveloped viruses, plays a role in retaining MBRs after cytokinesis. Knocking out BST2 leads to detachment of MBRs from cell surface and their transfer to distant cells, suggesting a new link between cytokinesis and viral biology.
Article
Virology
Chih-Yen Lin, Aspiro Urbina, Wen-Hung Wang, Arunee Thitithanyanont, Sheng-Fan Wang
Summary: Viral assembly and budding are the final and crucial steps of the virus life cycle, regulated by virus-host interaction. Many viruses exploit their late assembly (L) domains to manipulate host machinery for replication.
Review
Virology
Kevin M. Rose
Summary: Proper assembly and dissemination of progeny virions is crucial for virus replication, with viruses evolving various strategies to exploit cellular mechanisms for successful infection. Different viruses, such as retroviruses and herpesviruses, utilize host ESCRT complexes in distinct ways to ensure viral particle formation. Despite occurring in separate subcellular compartments and at different steps, the role of ESCRTs in viral lifecycles appears to be conserved.
Article
Biochemistry & Molecular Biology
Xiaohui Qiu, Yvan Campos, Diantha van de Vlekkert, Elida Gomero, Ajay C. Tanwar, Ravi Kalathur, Jason A. Weesner, Antonella Bongiovanni, Jeroen Demmers, Alessandra d'Azzo
Summary: This article reveals the mechanism of Alix homodimerization and its functions in cellular processes. The study shows that Alix can form dimers through disulfide bonds under physiological conditions and these dimers are enriched in exosomes and F-actin cytoskeleton. Furthermore, Alix plays an essential role in loading syntenin into exosomes and has different effects on cytoskeleton remodeling in its dimeric and monomeric forms.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
