Review
Biochemistry & Molecular Biology
Haim Breitbart, Elina Grinshtein
Summary: Capacitation is a process in which mammalian sperm undergo a series of biochemical reactions in the female reproductive tract, protecting the sperm from spontaneous acrosome reaction and ensuring successful fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
Elina Grinshtain, Sally Shpungin, Micha Baum, Uri Nir, Haim Breitbart
Summary: The physiological acrosome reaction occurs after the process of capacitation in mammalian spermatozoa. The study reveals a novel pathway involving the tyrosine kinase Fer that prevents spontaneous acrosome reaction by enhancing actin polymerization.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Reproductive Biology
Yael Azoulay, Zvi Malik, Haim Breitbart
Summary: Bacterial contamination in semen can deteriorate sperm function, potentially by inducing a premature form of the acrosome reaction. To understand the mechanism, the effects of bacteria on sperm acrosome reaction and other parameters involved in sperm capacitation were studied.
Article
Pharmacology & Pharmacy
Shehreen Amjad, Md Saidur Rahman, Won-Ki Pang, Do-Yeal Ryu, Elikanah Olusayo Adegoke, Yoo-Jin Park, Myung-Geol Pang
Summary: In this study, exposure to a phthalate mixture resulted in decreased sperm motility and hyperactivity, compromised sperm ability to generate ATP, induced abnormal capacitation and acrosome reaction, upregulated protein tyrosine phosphorylation via a protein kinase-A-dependent pathway, and significantly affected fertilization and early embryonic development. The findings suggest that the phthalate mixtures adversely affected sperm processes and ultimately led to poor fertilization rates and repressed embryonic development, with the lowest observed adverse effect dose being <1 µg/mL in vitro.
Article
Agriculture, Dairy & Animal Science
Vitaly Denisenko, Irena Chistyakova, Natalia Volkova, Ludmila Volkova, Baylar Iolchiev, Tatyana Kuzmina
Summary: Progesterone affects the functional status of bovine spermatozoa by inducing capacitation and acrosome reactions. Prolactin and dibutyryl cyclic adenosine monophosphate can modulate these effects in the presence of progesterone.
Review
Biochemistry & Molecular Biology
Filip Benko, Dana Urminska, Michal Duracka, Eva Tvrda
Summary: This review provides an overview of specific ion channels and transporters located in the mammalian sperm plasma membrane and their functional roles in sperm development and function. It discusses the potential therapeutic targets for infertility management.
Review
Biochemistry & Molecular Biology
Pauline Arbogast, Guillaume Gauchotte, Romane Mougel, Olivier Morel, Ahmed Ziyyat, Mikael Agopiantz
Summary: Neurotensin (NTS) is a peptide that was discovered in 1973 and has been extensively studied in various fields, particularly in oncology. This review focuses on its involvement in reproductive functions, specifically its role in ovulation and sperm function. NTS acts in an autocrine and paracrine manner, influencing the acrosome reaction in sperm and potentially improving the outcomes of in vitro fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Jinxiu Dong, Kejiao Pei, Wannan Xu, Mengmeng Gong, Wenrui Zhu, Siqi Liu, Min Tang, Jianjun Liu, Xingquan Xia, Xinjiang Bu, Liuwang Nie
Summary: This study identified the presence of turtle ZP genes and found that turtle ZP2, ZP3, and ZPD can induce acrosome exocytosis in reptilian spermatogenesis. It is the first report of the existence of gene duplication of turtle ZP genes and their significance in fertilization.
BIOLOGY OF REPRODUCTION
(2023)
Article
Agriculture, Dairy & Animal Science
Laura Robles-Gomez, Leopoldo Gonzalez-Brusi, Paula Saez-Espinosa, Natalia Huerta-Retamal, Paula Cots-Rodriguez, Manuel Aviles, Maria Jose Gomez-Torres
Summary: The study revealed that different in vitro capacitation times and acrosome reactions can affect the specific location of boar sperm glycoconjugates, showing distinct binding patterns for different lectins at different stages.
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Biology
Mohammad Mehedi Hasan, Qurat Ul Ain Re, Freddy Lattekivi, Janeli Viil, Kasun Godakumara, Keerthie Dissanayake, Aneta Andronowska, Ulle Jaakma, Alireza Fazeli
Summary: Before the union of an egg and spermatozoon, multiple vital processes occur in the female reproductive system. The maturation of spermatozoa in the female reproductive tract is crucial for fertilization. Follicular fluid (FF) and its extracellular vesicles (EVs) play a significant role in supporting sperm functions, with specific effects based on species and source of FF EVs. This study demonstrates that bovine FF EVs can support sperm function and contribute to a favorable periconceptional microenvironment.
Article
Biochemistry & Molecular Biology
Alejandra A. Covarrubias, Erwin De la Fuente-Ortega, Gabriela Rossi, Ennio Cocca, Mose Rossi, Gianna Palmieri, Floria C. Pancetti
Summary: This study characterized the distribution and activity of APEH during spermatogenesis, revealing differential levels of APEH in various cell types and suggesting a potential role in oxidative stress protection and sperm capacitation.
Article
Andrology
Luisa Ramirez-Agamez, Camilo Hernandez-Aviles, Isabel Ortiz, Charles C. Love, Dickson D. Varner, Katrin Hinrichs
Summary: This study demonstrates that incubation with a specific energy substrate, lactate, is associated with a spontaneous acrosome reaction in stallion spermatozoa. The proportion of live, acrosome-reacted spermatozoa obtained is among the highest reported for equine sperm. These findings contribute to our understanding of stallion sperm physiology.
Article
Agriculture, Dairy & Animal Science
Phillip H. Purdy, James K. Graham, Hymerson C. Azevedo
Summary: This study used flow cytometry to evaluate the viability and capacitation of sperm and investigated the effects of different freezing and capacitating media. The results showed that different media induced different responses in sperm capacitation and function.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
David Martin-Hidalgo, Beatriz Macias-Garcia, Lauro Gonzalez-Fernandez
Summary: Increasing sperm concentration stimulates capacitation and acrosome reaction in boars, accompanied by plasma membrane lipid disorganization and increased sperm viability.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2022)
Article
Biochemistry & Molecular Biology
Cesar I. Ortiz-Garcia, Monica L. Salgado-Lucio, Ana L. Roa-Espitia, Aide A. Munoz-Sanchez, Joaquin Cordero-Martinez, Enrique O. Hernandez-Gonzalez
Summary: This study found that guinea pig and mouse spermatozoa express NOX2 and NOX4 during capacitation, and these enzymes play an important role in ROS production. The study also discovered an interaction between NOX2 and NOX4, which depends on the activation of calpain. The results indicate that NOX2 and NOX4 are the most important ROS producers during guinea pig and mouse sperm capacitation, and their activation depends on calpain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)