Article
Biology
Robert Philip Ganley, Marilia Magalhaes de Sousa, Kira Werder, Tugce Ozturk, Raquel Mendes, Matteo Ranucci, Hendrik Wildner, Hanns Ulrich Zeilhofer
Summary: Using viral strategies and genetic techniques, researchers identified two distinct populations of serotonergic hindbrain neurons in mice that innervate different spinal cord regions and have opposing effects on pain sensitivity. Activation of neurons in the lateral paragigantocellularis (LPGi) reduces thermal sensitivity, while activation of medial hindbrain neurons increases sensitivity to mechanical stimulation.
Article
Biochemistry & Molecular Biology
Yu-Lung Lin, Jennifer Nhieu, Pei-Yao Liu, Gengyun Le, Dong Jun Lee, Chin-Wen Wei, Yi-Wei Lin, Sang-Hyun Oh, Dawn Lowe, Li-Na Wei
Summary: This study identifies the role of cellular retinoic acid-binding protein 1 (CRABP1) in the formation and maintenance of the neuromuscular junction (NMJ) and highlights the regulatory role of CRABP1-CaMKII-Agrn signaling in NMJ. It also suggests a potential protective role of CRABP1 in MN diseases.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Biochemistry & Molecular Biology
Brian Osmond, Caroline O. B. Facey, Chi Zhang, Bruce M. Boman
Summary: This study reveals that HOXA9 may play a crucial role in the stem cell origin of colorectal cancer (CRC) by regulating the retinoic acid (RA) signaling pathway. Dysregulated RA signaling leads to overexpression of HOXA9, which contributes to the overpopulation of cancer stem cells (CSCs) in CRC. The findings provide insights into a potential regulatory mechanism and therapeutic target for controlling CSC overpopulation in CRC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Dan Tang, Zhao Zhang, Emily Zboril, Michael D. Wetzel, Xinping Xu, Wei Zhang, Lizhen Chen, Zhijie Liu
Summary: Pontin is a crucial protein involved in various biological processes, such as gene transcription regulation and chromatin remodeling, particularly playing a key role in RA-induced HOX gene activation. Through Pontin knockdown experiments and genome-wide binding pattern studies, its mechanism in regulating HOX gene expression at the transcriptional level has been revealed.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Sandeep Gupta, Riki Kawaguchi, Eric Heinrichs, Salena Gallardo, Stephanie Castellanos, Igor Mandric, Bennett G. Novitch, Samantha J. Butler
Summary: This study successfully generated a large quantity of bona fide somatosensory interneurons using mouse embryonic stem cells. The findings suggest that retinoic acid and bone morphogenetic protein 4 can induce the differentiation of cells into different types of spinal interneurons, and activating Wnt signaling can enhance neural progenitor proliferation and yield.
Article
Anatomy & Morphology
Tatsuya Yuikawa, Takehisa Sato, Masaaki Ikeda, Momo Tsuruoka, Kaede Yasuda, Yuto Sato, Kouhei Nasu, Kyo Yamasu
Summary: In this study, the involvement of the pou5f3 gene in spinal cord elongation and neurogenesis was confirmed through mutant analysis and gain-of-function experiments. The study also revealed that pou5f3 regulates retinoic acid (RA) levels, which contribute to neurogenesis in the posterior neural tube.
DEVELOPMENTAL DYNAMICS
(2023)
Article
Multidisciplinary Sciences
Aurelien Villedieu, Lale Alpar, Isabelle Gaugue, Amina Joudat, Francois Graner, Floris Bosveld, Yohanns Bellaiche
Summary: This study reveals that the interplay between genetic patterning and tissue geometry drives morphogenesis during development. The invagination of the Deformed (Dfd) homeotic compartment plays a crucial role in the formation of the fold separating the adult Drosophila head and thorax segments. The introduction of curvature heterogeneity within the homeotic compartment converts the Dfd-dependent in-plane tension into an inward force driving folding.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Marie Berenguer, Gregg Duester
Summary: The role of retinoic acid (RA) and Meis1/2 in limb development is complex, with RA activating Meis1/2 in the proximal limb bud mesoderm while FGF8 represses Meis1/2 in the distal limb to generate proximodistal patterning.
Article
Developmental Biology
Axelle Wilmerding, Lucrezia Rinaldi, Nathalie Caruso, Laure Lo Re, Emilie Bonzom, Andrew J. Saurin, Yacine Graba, Marie-Claire Delfini
Summary: The HoxB genes have a generic function in the developing spinal cord, controlling the expression of Lzts1 and neuronal delamination.
Article
Cell Biology
Mateus C. Adolfi, Amaury Herpin, Anabel Martinez-Bengochea, Susanne Kneitz, Martina Regensburger, David J. Grunwald, Manfred Schartl
Summary: Sex determination mechanism is associated with retinoic acid (RA) and Stra8, affecting germ cell development. RA treatments may inhibit germ cell differentiation and lead to precocious meiosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marta Melis, Xiao-Han Tang, Nabeel Attarwala, Qiuying Chen, Carlos Prishker, Lihui Qin, Steven S. Gross, Lorraine J. Gudas, Steven E. Trasino
Summary: The study showed that the use of the retinoic acid receptor agonist AC261 could reduce the severity of alcohol liver disease (ALD) and decrease liver damage. Although AC261 did not prevent alcohol-induced hepatic retinoid depletion, it was able to reduce the increase in serum retinol caused by alcohol. These findings suggest the potential of AC261 in preventing ALD.
Review
Cell Biology
Nikhil Hajirnis, Rakesh K. Mishra
Summary: Hox genes, responsible for regulating the anterior-posterior body axis in animals, exhibit diversity in arrangement across different organisms. Minor changes in gene expression levels play a significant role in the development of biological features, impacting primary axis formation, segment identity, and other crucial processes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Pascal Schmidt, Eva Leman, Ronan Lagadec, Michael Schubert, Sylvie Mazan, Ram Reshef
Summary: This study investigates the role of retinoic acid (RA) in the regulation of chordate and vertebrate pronephros formation through a comparative developmental approach. The results show that the conservation of Pax2 and Lim1 expressions in chordate pronephros homologs is present, but the responsiveness to RA and Hox-dependent regulation is a novelty in gnathostomes.
Article
Developmental Biology
Zainab Afzal, Jeffrey J. Lange, Christof Nolte, Sean McKinney, Christopher Wood, Ariel Paulson, Bony De Kumar, Jay Unruh, Brian D. Slaughter, Robb Krumlauf
Summary: Signaling pathways regulate the patterns of Hox gene expression in axial identity specification. Little is known about the properties of cis-regulatory elements and transcriptional mechanisms that control Hox expression through graded signaling inputs. This study optimized a single molecule fluorescent in situ hybridization (smFISH) technique to evaluate the role of three retinoic acid response element (RARE)-dependent enhancers in regulating nascent transcription patterns of Hoxb genes. The results suggest that these enhancers have differential impacts on global and local patterns of transcription, and their competitive interactions are important for maintaining proper levels and patterns of Hoxb transcription.
Article
Chemistry, Applied
Kun Liu, Xianzhen Dong, Yue Wang, Xiaopei Wu, Honglian Dai
Summary: A dopamine-modified chitosan hydrogel was designed to improve the microenvironment of spinal cord injury. In vitro and in vivo experiments demonstrated that the dopamine-modified hydrogel enhanced cell survival, modulated immune response, and promoted axonal regeneration in injured spinal cord.
CARBOHYDRATE POLYMERS
(2022)
Article
Anatomy & Morphology
Albert G. Hayward, Piyush Joshi, Isaac Skromne
DEVELOPMENTAL DYNAMICS
(2015)
Article
Materials Science, Biomaterials
Shanghao Li, Isaac Skromne, Zhili Peng, Julia Dallman, Abdulrahman O. Al-Youbi, Abdulaziz S. Bashammakh, Mohammad S. El-Shahawi, Roger M. Leblanc
JOURNAL OF MATERIALS CHEMISTRY B
(2016)
Article
Chemistry, Multidisciplinary
Zhili Peng, Esmail H. Miyanji, Yiqun Zhou, Joel Pardo, Sajini D. Hettiarachchi, Shanghao Li, Patricia L. Blackwelder, Isaac Skromne, Roger M. Leblanc
Article
Genetics & Heredity
Kemal O. Yariz, Duygu Duman, Celia Zazo Seco, Julia Dallman, Mingqian Huang, Theo A. Peters, Asli Sirmaci, Na Lu, Margit Schraders, Isaac Skromne, Jaap Oostrik, Oscar Diaz-Horta, Juan I. Young, Suna Tokgoz-Yilmaz, Ozlem Konukseven, Hashem Shahin, Lisette Hetterschijt, Moien Kanaan, Anne M. M. Oonk, Yvonne J. K. Edwards, Huawei Li, Semra Atalay, Susan Blanton, Alexandra A. DeSmidt, Xue-Zhong Liu, Ronald J. E. Pennings, Zhongmin Lu, Zheng-Yi Chen, Hannie Kremer, Mustafa Tekin
AMERICAN JOURNAL OF HUMAN GENETICS
(2012)
Article
Developmental Biology
Keun Lee, Isaac Skromne
Meeting Abstract
Developmental Biology
Jessie Chang, Isaac Skromne, Robert Ho
DEVELOPMENTAL BIOLOGY
(2009)
Meeting Abstract
Developmental Biology
Mejdi Najjar, Bryant Huang, Isaac Skromne
DEVELOPMENTAL BIOLOGY
(2011)
Meeting Abstract
Developmental Biology
Jessie Chang, Isaac Skromne, Robert Ho
DEVELOPMENTAL BIOLOGY
(2011)
Review
Anatomy & Morphology
Isaac Skromne, Victoria E. Prince
DEVELOPMENTAL DYNAMICS
(2008)
Article
Developmental Biology
Piyush Joshi, Andrew J. Darr, Isaac Skromne
DEVELOPMENTAL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Piyush Joshi, Isaac Skromne
Article
Pharmacology & Pharmacy
Rachel DuMez, Esmail H. Miyanji, Lesly Corado-Santiago, Bryle Barrameda, Yiqun Zhou, Sajini D. Hettiarachchi, Roger M. Leblanc, Isaac Skromne
Summary: Developing novel chemical carriers to deliver drug agents specifically to bones may be an effective approach for treating adult bone diseases.
Meeting Abstract
Developmental Biology
Claudio D. Stern, Octavian Voiculescu, Federica Bertocchini, Isaac Skromne, Ray E. Keller
DEVELOPMENTAL BIOLOGY
(2007)
Article
Developmental Biology
Isaac Skromne, Dean Thorsen, Melina Hale, Victoria E. Prince, Robert K. Ho
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)