Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 59, Issue -, Pages 25-33Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2015.12.021
Keywords
PAP; Ribosomal protein L26; Carp; Phagocytosis; Aeromonas hydrophila; Chitosan
Categories
Funding
- National Research University Project of Thailand
- Office of the Higher Education Commission
- National Research Council of Thailand (NRCT)
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Cyprinus carpio (koi) is one of the most popular ornamental fish. A major problem for C. carpio farming is bacterial infections especially by Aeromonas hydrophila. Previously studies had shown that the Phagocytosis Activating Protein (PAP) gene was involved in the innate immune response of animals. Therefore, we attempted to identify a role for the PAP gene in the immunology of C. carpio. The expression of the PAP was found in C. carpio whole blood and increased when the fish were stimulated by inactivated A. hydrophila. In addition, PAP-phMGFP DNA was injected as an immunostimulant. The survival rate and the phagocytic index were significantly increased in the A. hydrophila infected fish that received the PAP-phMGFP DNA immunostimulant. A chitosan-PAP-phMGFP nanoparticle was then developed and feeded into fish which infected with A. hydrophila. These fish had a significantly lower mortality rate than the control. Therefore, this research confirmed a key role for PAP in protection fish from bacterial infection and the chitosan-PAP-phMGFP nanoparticle could be a good prototype for fish immunostimulant in the future. (C) 2016 Elsevier Ltd. All rights reserved.
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