Article
Environmental Sciences
Jia-Yong Lao, Ting-Yu Li, Rongben Wu, Yuefei Ruan, Eddy Y. Zeng, Jiaxue Wu, Paul K. S. Lam
Summary: This study focuses on the distribution and sources of size-segregated PAHs in the Pearl River Estuary, revealing variations in PAH content and sources between different sites. It also explores the distribution and deposition velocities of PAHs on particles of different sizes. Additionally, the study reveals differences in behavior during air-water exchange of PAHs, providing new insights into the sources and fate of PAHs in the atmosphere.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Taekeon Jung, Young-Ran Yun, Jaehyun Bae, Sung Yang
Summary: By integrating magnetic particle conjugation for bacteria concentration and separation, a new impedimetric bacteria-detection sensor has been developed for rapid and accurate detection of low bacteria concentrations in food samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Environmental
Wan Hanna Melini Wan Mohtar, Muhammad Amirul Mohd Razali, Muhammad Abid Mazlan, Arash Zulkarnain Ahmad Rozaini, Silambarasi A. P. Mooralitharan, Aidil Abdul Hamid, Muhamad Ramdzan Buyong
Summary: This study investigates the feasibility of using dielectrophoresis (DEP) electrode to detect ESKAPE and enteric bacteria in water samples. By recording the response of bacteria to the non-uniform electric field in micro tapered DEP electrode and microfluidic chamber at varying frequencies, the positive, negative, and transitional electrokinetic responses of each bacterium can be determined. DEP technique successfully detects ESKAPE and enteric bacteria in water samples, with the highest microbial contamination found in river water in the urban water cycle.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2023)
Article
Multidisciplinary Sciences
Maliha Saleem Bakhshi, Mohsin Rizwan, Ghulam Jilany Khan, Hong Duan, Kefeng Zhai
Summary: In this study, a novel two-stage, label-free, rapid, and continuous CTC separation device based on hydrodynamic inertial focusing and dielectrophoretic separation is proposed. The device can effectively separate CTCs from other blood cells, yielding viable CTCs with high efficiency.
SCIENTIFIC REPORTS
(2022)
Article
Physics, Applied
Mohammad Asif Zaman, Mo Wu, Wei Ren, Michael A. Jensen, Ronald W. Davis, Lambertus Hesselink
Summary: This article introduces an optoelectronic tweezer (OET) device that provides improved trapping resolution for a given optical spot size. The device uses patterned physical electrodes to create an asymmetric electric field gradient, resulting in an additional azimuthal force component along with the conventional radial gradient force. The device achieves stable force equilibrium at a pair of antipodal points surrounding the optical beam. Unlike conventional OETs, which allow trapping at any point around the beam perimeter, this scheme enhances resolution by limiting trapping to two points. The working principle is analyzed through numerical analysis of electromagnetic fields and corresponding forces. Experimental results demonstrate successful trapping and manipulation of micro-particles using the proposed device.
APPLIED PHYSICS LETTERS
(2023)
Article
Multidisciplinary Sciences
Yunfeng Ding, Yonghong Liu, Dong-Kee Lee, Zhangwei Tong, Xiaobin Yu, Yi Li, Yong Xu, Rainer B. Lanz, Bert W. O'Malley, Jianming Xu
Summary: This study reveals that ERα*RFP*Erbb2* cells progressively lose ERα during tumorigenesis to become highly metastatic, and these ERα*origin breast cancers are more aggressive.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Engineering, Chemical
Reza Derakhshan, Amirhosein Ghasemi, Reza Moradi, Abas Ramiar
Summary: This paper presents a comprehensive numerical investigation on particle/cell separation in circular microchannels using dielectrophoresis (DEP). The results show that the direction of particle deflection can be controlled by varying the geometrical parameters. By applying DEP, efficient separation of particles and cells based on their size and physical properties is achieved.
ADVANCED POWDER TECHNOLOGY
(2023)
Article
Engineering, Biomedical
Hyuna Kim, Anna Wirasaputra, Farnaz Mohammadi, Aritra Nath Kundu, Jennifer A. E. Esteves, Laura M. M. Heiser, Aaron S. S. Meyer, Shelly R. R. Peyton
Summary: Breast cancer is a major cause of cancer-related deaths worldwide, primarily due to metastasis and dormancy. Dormancy, a prolonged period between tumor resection and relapse, poses significant challenges in current therapies. To overcome this, a live cell lineage approach is used to track and distinguish dormant cells from proliferative and dying cells in various in vitro microenvironments. The study reveals that the proportion of long-term quiescent cells is regulated by both intrinsic and extrinsic factors, with the highest percentage found in 3D collagen gels. This approach is expected to benefit the study of dormant tumor cells for biologists and bioengineers in the dormancy community.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Analytical
Fengtao Jiang, Nan Xiang
Summary: This paper presents the development of a novel handheld sorter with a multifunction integrated microfluidic chip for point-of-care diagnosis in resource-limited settings. The sorter utilizes a spiral inertial microfluidic channel and flow regulators for size-based cell separation. The prototype of the sorter successfully separates malignant tumor cells from clinical pleural effusions. The device is simple to use, inexpensive, portable, and compact, making it suitable for high-throughput label-free cell separation in resource-limited areas.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Malihe Farasat, Ehsan Aalaei, Saeed Kheirati Ronizi, Atin Bakhshi, Shaghayegh Mirhosseini, Jun Zhang, Nam-Trung Nguyen, Navid Kashaninejad
Summary: Separation and detection of cells and particles in a suspension are essential for various applications, including biomedical investigations and clinical diagnostics. Microfluidics has enabled the miniaturization of analytical devices by controlling the motion of fluid in micrometer-sized channels and chambers. Among different microfluidic methods, dielectrophoresis (DEP) offers advantages for manipulating neutral bioparticles using unique dielectric characteristics. This review focuses on signal-based DEP methods that utilize signal parameters such as frequency, amplitude, phase, and shape for cell/particle separation and manipulation.
Article
Chemistry, Analytical
Jino Fathy, Yongjun Lai
Summary: This paper presents a dynamic-mode microcantilever sensor based on a gap method. By applying ac electric potential and dielectrophoresis force, the sensor can detect E. coli bacteria in real-time, with the concentration and electric potential amplitude affecting the frequency shifts.
Article
Biochemical Research Methods
Kazuma Yoda, Yoshiyasu Ichikawa, Masahiro Motosuke
Summary: This paper presents a newly developed high-throughput measurement device for determining the dielectric properties of cancer cells. The device utilizes continuous-flow electrorotation to measure the rotational behavior of cells, resulting in a significant improvement in throughput compared to conventional devices.
Article
Biochemistry & Molecular Biology
Elena Vinuesa-Pitarch, Daniel Ortega-Alvarez, Veronica Rodilla
Summary: This article reviews the application of lineage tracing analysis in breast cancer research, discussing the importance of cellular heterogeneity and the strategies to identify the cell of origin in breast cancer subtypes. It also examines the role of cellular plasticity in tumorigenesis.
Article
Physics, Applied
Binzhen Zhang, Yangyang Wang, Yunpeng Liu, Huan Li, Wenxuan Zang, Junping Duan
Summary: This paper proposes a novel focusing method using microfilters and interdigital electrodes to capture and separate particles. Experimental results show that the capture efficiency of the chip reaches 71%-86% and the separation efficiency is over 98.6%, making it potentially suitable for separating circulating tumor cells in blood samples.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Chemistry, Analytical
Ryu Nakabayashi, Masanori Eguchi
Summary: This paper proposes a modified iDEP device for single-cell analysis. The device is capable of measuring particle velocity at a wide frequency range and produces consistent results.
Article
Mechanics
Yao Xin, Ng Chyi Huey, Jia Rui Amanda Teo, Marcos, Wong Teck Neng
JOURNAL OF FLUID MECHANICS
(2019)
Article
Limnology
Xinhui Shen, Marcos, Henry C. Fu
LIMNOLOGY AND OCEANOGRAPHY
(2020)
Article
Engineering, Multidisciplinary
Xin Yao, Marcos
APPLIED MATHEMATICAL MODELLING
(2020)
Article
Biology
Pei Yen Tan, Marcos, Yu Liu
JOURNAL OF THEORETICAL BIOLOGY
(2020)
Article
Biochemical Research Methods
Tian Fook Kong, Xinhui Shen, Marcos, Chun Yang, Imran Halimi Ibrahim
Article
Engineering, Chemical
Xin Yao, Marcos
Summary: This study presents a systematic procedure to analyze the interaction between two spheres in a porous medium under a uniform electric field. The results show that the sphere interaction is dependent on parameters such as separation distance, sphere radii, zeta potential, and normalized radius with respect to permeability. An increase in the normalized radius with respect to permeability reduces the interaction between the two spheres, especially when the normalized radius is relatively small (αa < 50).
CHEMICAL ENGINEERING SCIENCE
(2021)
Correction
Biochemical Research Methods
Jiyu Li, Ya Liu, Jifeng Ren, Benjamin Zikai Tay, Tao Luo, Lei Fan, Dong Sun, Guannan Luo, Denvid Lau, Marcos, Raymond H. W. Lam
Article
Limnology
Xinhui Shen, Xin Yao, Marcos, Henry C. Fu
Summary: In this study, the feeding behavior of copepods and the role of setae in prey detection were investigated. It was found that in the presence of prey, setae deformations are more pronounced and larger prey cause greater flow disturbances.
LIMNOLOGY AND OCEANOGRAPHY
(2021)
Article
Biophysics
Xinhui Shen, Phu N. Tran, Benjamin Z. Tay, Marcos
Summary: This study investigates the role of different subunit domains in regulating the rigidity of bacterial flagellar filaments. The results show that the absence of two outer subunit domains has little effect on the bending stiffness of Bacillus subtilis' flagellar filament, suggesting that the rigidity mainly comes from the inner domains.
BIOPHYSICAL JOURNAL
(2022)
Article
Chemistry, Physical
Shuai Yin, Yi Huang, Xinhui Shen, Chaoyang Zhang, Xue Chen, Marcos, Haiwang Li, Teck Neng Wong
Summary: This study explores a new method for the fabrication of multilayer capsules and successfully produces defect-free capsules with a solid outer shell. Experimental results demonstrate that these capsules not only provide reliable protection for the core, but also offer great potential for customization of capsule functionalities.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Nanoscience & Nanotechnology
Ting Wei Teo, Xinhui Shen, Pei Yen Tan, Marcos
Summary: This study presents a real-time microfluidic device for measuring the effects of bacteria deposition on the permeability of porous media. The experiments reveal that the percentage of trapped cells is independent of bacterial density, and the reduction in permeability follows an exponential trend with an approaching pseudo-steady state. The study also finds that bacteria preferentially deposit in regions with larger voids within the porous medium.
MICROFLUIDICS AND NANOFLUIDICS
(2022)
Article
Chemistry, Analytical
Xinhui Shen, Ting Wei Teo, Tian Fook Kong, Marcos
Summary: This article presents a microfluidic technique for rapid enumeration of bacterial density using a syringe filter. The technique quantifies bacterial density through pressure difference measurement across the membrane. The article demonstrates the accuracy and speed of the method in determining bacterial density through experimental validation.
Article
Multidisciplinary Sciences
Jiyu Li, Dinglong Hu, Chee Kent Lim, Jifeng Ren, Xin Yao, Chao Ma, Marcos, Weiqiang Chen, Patrick K. H. Lee, Raymond H. W. Lam
Summary: The human host-associated microbial communities can provide insights into health status, and single-cell bacteria identification can serve as reliable biomarkers for clinical diagnosis. However, existing single-cell isolation methods often face challenges in obtaining sufficient sample volume and target cells. This research presents a novel microfluidic system for single-bacteria extraction, which shows high success rates and compatibility with downstream analysis. It demonstrates the potential application of this system in microbiome and pathology research, as well as clinical diagnosis.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Jiyu Li, Ya Liu, Jifeng Ren, Benjamin Zikai Tay, Tao Luo, Lei Fan, Dong Sun, Guannan Luo, Denvid Lau, Marcos, Raymond H. W. Lam