Article
Biochemistry & Molecular Biology
Angela Fernandez-Iglesias, Rocio Fuente, Helena Gil-Pena, Laura Alonso-Duran, Fernando Santos, Jose Manuel Lopez
Summary: The study investigated the formation of the epiphyseal bone plate using various microscopy techniques and found that the bone tissue is generated through a process different from the usual endochondral ossification. Osteoblasts deposit bone matrix on various surfaces, leading to the establishment of a core of bone sheet and subsequent thickening and reshaping. The processes occur in close relationship with cartilage, providing insight into the complex cellular events underlying bone formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Toshihisa Komori
Summary: Runx2 is a crucial transcription factor in bone development, playing a key role in the maturation and proliferation of chondrocytes and osteoblasts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Gretl Hendrickx, Verena Fischer, Astrid Liedert, Simon von Kroge, Melanie Haffner-Luntzer, Laura Brylka, Eva Pawlus, Michaela Schweizer, Timur Yorgan, Anke Baranowsky, Tim Rolvien, Mona Neven, Udo Schumacher, David J. Beech, Michael Amling, Anita Ignatius, Thorsten Schinke
Summary: The skeleton is a dynamic tissue that continuously adapts to mechanical stimuli. Piezo1 plays a critical role in bone formation, specifically in endochondral ossification. Deletion of Piezo1 may lead to osteoporosis and spontaneous fractures.
JOURNAL OF BONE AND MINERAL RESEARCH
(2021)
Review
Cell Biology
Hui Chen, Xiao-Ning Tan, Shi Hu, Ren-Qin Liu, Li-Hong Peng, Yong-Min Li, Ping Wu
Summary: Cartilage is a connective tissue essential for joint movement and pressure buffering, but lacks self-recovery ability. Repair of cartilage injury involves stimulating chondrocyte proliferation and ECM synthesis. Cartilage homeostasis regulation and cellular signaling transduction are complex processes with potential implications for cartilage injury treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Tsz Long Chu, Peikai Chen, Anna Xiaodan Yu, Mingpeng Kong, Zhijia Tan, Kwok Yeung Tsang, Zhongjun Zhou, Kathryn Song Eng Cheah
Summary: This study reveals that MMP14 plays a crucial role in the regulation of PTH signaling, thus contributing to bone homeostasis. During the development of osteoblasts from HC-descendent cells, MMP14 and the PTH pathway are activated. MMP14 cleaves PTH1R to dampen PTH signaling, thereby regulating bone synthesis.
Review
Biochemistry & Molecular Biology
Megan N. Michalski, Bart O. Williams
Summary: This review discusses the development and application of genetically engineered mouse models in musculoskeletal biology research, with a particular focus on tissue-specific gene knockout and the impact of CRISPR/Cas technology.
Review
Biochemistry & Molecular Biology
Shawn A. Hallett, Wanida Ono, Renny T. Franceschi, Noriaki Ono
Summary: “The cranial base, formed by endochondral ossification, plays a crucial role in cranial elongation and overall craniofacial growth. The cranial base contains synchondroses with unique developmental origins, and premature ossification of these synchondroses can lead to midfacial hypoplasia in humans. Major signaling pathways and transcription factors involved in long bone growth plate also play a role in the cranial base synchondrosis. This paper provides an updated overview of the cranial base synchondrosis, including its cell population and molecular regulation, and discusses future research opportunities in understanding its unique function in craniofacial skeletal structure.”
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Haoran Chen, Yujia Cui, Demao Zhang, Jing Xie, Xuedong Zhou
Summary: This review systematically summarizes studies published in the past 5 years that assess the relationship between FGF-8 and chondrocytes, revealing the regulatory role of FGF-8 in chondrocyte proliferation, differentiation, survival, and migration. Based on the analysis of existing research results, a therapeutic regimen targeting FGF-8 is proposed to explore the possibility of treating chondrocyte-related diseases.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Review
Dentistry, Oral Surgery & Medicine
R. S. Marcucio, T. Miclau, C. S. Bahney
Summary: This review discusses the relationship between mechanisms of bone formation and bone regeneration, highlighting that regeneration is not simply a reinduction of the molecular and cellular programs used for development. The mechanical environment plays a significant role in repair, while cell-intrinsic processes drive skeletal formation.
JOURNAL OF DENTAL RESEARCH
(2023)
Review
Endocrinology & Metabolism
Gongsheng Yuan, Shuying Yang
Summary: This review summarizes the role of RGS proteins in bone homeostasis and diseases, focusing on their regulation of osteoblasts, chondrocytes, and osteoclasts. The impacts of RGS proteins on bone development and related diseases are highlighted, suggesting their potential as drug targets.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Multidisciplinary Sciences
Sebastian Mathes, Alexandra Fahrner, Umesh Ghoshdastider, Hannes A. Ruediger, Michael Leunig, Christian Wolfrum, Jan Kruetzfeldt
Summary: The research indicates that FGF-2 plays a dual role in promoting muscle growth and intramuscular adipogenesis, and activates the miR29a/SPARC axis to regulate differentiation of fibro/adipogenic progenitors and intramuscular adipose tissue formation in aged skeletal muscle.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Endocrinology & Metabolism
Dima W. Alhamad, Husam Bensreti, Jennifer Dorn, William D. Hill, Mark W. Hamrick, Meghan E. McGee-Lawrence
Summary: The AhR plays critical roles in regulating bone cell biology, including osteoblasts, osteoclasts, the immune system, and mesenchymal stem cell differentiation. It responds to both environmental pollutants and endogenous ligands, affecting signaling cascades and downstream effects. Understanding the mechanisms of AhR in bone is important for addressing skeletal disorders.
JOURNAL OF MOLECULAR ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wangyuan Yao, Muhammad Fakhar-e-Alam Kulyar, Yanmei Ding, Haitao Du, Jiajia Hong, Kyein San Loon, Shah Nawaz, Jiakui Li
Summary: The interaction between miR-140 and HDAC4 plays a crucial role in bone disorders such as tibial dyschondroplasia. Low miR-140 expression is associated with poor pathological characteristics and low chondrocyte survival. HDAC4 inhibits MEF2C and Runx2 and interacts with Col-II, Col-X, and COMP, leading to uncontrolled chondrocyte proliferation, differentiation, and dysplastic growth. Targeting the miR-140 and HDAC4 feedback loop may provide a promising therapeutic approach for chondrocyte-related bone disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Xiankun Cao, Wenxin He, Kewei Rong, Shenggui Xu, Zhiqian Chen, Yuwei Liang, Shuai Han, Yifan Zhou, Xiao Yang, Hui Ma, An Qin, Jie Zhao
Summary: This study revealed that DZNep enhances osteoblast differentiation and mineralization through the EZH2-H3K27me3-Wnt4 axis, while also promotes osteoclast formation via the EZH2-H3K27me3-Foxc1 axis. This enhanced osteogenesis and osteoclastogenesis resulted in accelerated bone defect healing in mice.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Cheng Ma, Huan Liu, Yifan Wei, He Li, Dengshun Miao, Yongxin Ren
Summary: This study investigated the effects of exogenous parathyroid hormone (PTH) and endogenous PTH deficiency on fracture healing and explored the underlying mechanisms. The results showed that exogenous PTH promoted bone formation and improved impaired fracture healing resulting from endogenous PTH deficiency by activating the IHH pathway and accelerating endochondral ossification. Therefore, understanding the mechanism of PTH treatment on fracture repair could guide the development of effective therapeutic targets for fractures.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Anatomy & Morphology
Andrew S. Hagan, Michael Boylan, Craig Smith, Estela Perez-Santamarina, Karolina Kowalska, Irene H. Hung, Renate M. Lewis, Mohammad K. Hajihosseini, Mark Lewandoski, David M. Ornitz
DEVELOPMENTAL DYNAMICS
(2019)
Article
Genetics & Heredity
Lu M. Yang, Kathryn S. E. Cheah, Sung-Ho Huh, David M. Ornitz
Article
Developmental Biology
Andrew S. Hagan, Bo Zhang, David M. Ornitz
Article
Biochemistry & Molecular Biology
Yongjun Yin, David M. Ornitz
Article
Developmental Biology
Emily M. A. Lewis, Savita Sankar, Caili Tong, Ethan S. Patterson, Laura E. Waller, Paul Gontarz, Bo Zhang, David M. Ornitz, Kristen L. Kroll
DEVELOPMENTAL BIOLOGY
(2020)
Article
Anatomy & Morphology
Lu M. Yang, Lisa Stout, Michael Rauchman, David M. Ornitz
DEVELOPMENTAL DYNAMICS
(2020)
Article
Anatomy & Morphology
Yutao Su, Lu M. Yang, David M. Ornitz
Summary: The study indicates that both MAPK and PI3K pathways are crucial downstream mediators of FGF20-FGFR1 signaling in cochlear hair cell and supporting cell differentiation.
DEVELOPMENTAL DYNAMICS
(2021)
Article
Anatomy & Morphology
Connor C. Leek, Jaclyn M. Soulas, Iman Bhattacharya, Elahe Ganji, Ryan C. Locke, Megan C. Smith, Jaysheel D. Bhavsar, Shawn W. Polson, David M. Ornitz, Megan L. Killian
Summary: This study investigated the role of Fgf9 in regulating the size of the deltoid tuberosity, revealing that Fgf9 deficiency affected cartilage growth and mineralization, as well as transcriptional profiles of skeletal muscle. The findings suggest that Fgf9 may play a crucial role in muscle-bone cross-talk during both embryonic and postnatal development.
DEVELOPMENTAL DYNAMICS
(2021)
Article
Cell Biology
Jessica Weant, David D. Eveleth, Amuthakannan Subramaniam, Jennifer Jenkins-Eveleth, Michael Blaber, Ling Li, David M. Ornitz, Asaf Alimardanov, Trevor Broadt, Hui Dong, Vinay Vyas, Xiaoyi Yang, Ralph A. Bradshaw
Summary: The two human FGF1 derivatives showed promising results in stimulating rabbit corneal endothelial cell proliferation, indicating their potential for regenerating corneal endothelial deficits in humans.
Article
Biochemistry & Molecular Biology
Elahe Ganji, Connor Leek, William Duncan, Debabrata Patra, David M. Ornitz, Megan L. Killian
Summary: This study investigates the role of Fgf9 in the development and function of the postnatal enthesis. By conditional knockout of Fgf9 in mouse tendons and entheses, the researchers found that Fgf9 is important for the structural and functional development of the enthesis. Mice with Fgf9 knockout showed thinner tendons, reduced bone mineralization, increased cellularity, and decreased mechanical properties in the entheses.
Article
Cardiac & Cardiovascular Systems
Dzmitry Matsiukevich, Attila Kovacs, Tiandao Li, Kristen Kokkonen-Simon, Scot J. Matkovich, Sunday S. Oladipupo, David M. Ornitz
Summary: Heart failure (HF) is a common and serious condition, particularly in older adults and patients with multiple metabolic comorbidities. Heart failure with preserved ejection fraction (HFpEF) is a clinical syndrome characterized by organ dysfunction and symptoms of heart failure, despite a normal or near-normal left ventricular ejection fraction. In this study, researchers successfully created a rodent model with a robust HFpEF phenotype, exhibiting characteristics and manifestations similar to the human disease.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Anatomy & Morphology
Kendra K. Wernle, Michael A. Sonnenfelt, Connor C. Leek, Elahe Ganji, Anna Lia Sullivan, Claudia Offutt, Jordan Shuff, David M. Ornitz, Megan L. Killian
Summary: The study reveals that FGFR signaling plays a critical role in regulating the growth and maintenance of tendon/ligament attachments and the size and shape of bony eminences.
DEVELOPMENTAL DYNAMICS
(2023)
Review
Medicine, Research & Experimental
David M. Ornitz, Nobuyuki Itoh
Summary: This review provides an overview of the fibroblast growth factor (FGF) family and focuses on new developments in the field since 2015. Highlights of the past 6 years include advancements in experimental modulation of FGF signaling, clinical use of small molecule FGFR inhibitors, expanded understanding of endocrine FGF signaling, and the roles of FGF signaling in stem cells, tissue homeostasis, regeneration, and neuropsychiatric diseases.
WIRES MECHANISMS OF DISEASE
(2022)
Article
Medicine, Research & Experimental
Kel Vin Woo, Isabel Y. Shen, Carla J. Weinheimer, Attila Kovacs, Jessica Nigro, Chieh-Yu Lin, Murali Chakinala, Derek E. Byers, David M. Ornitz
Summary: Research suggests that activation of endothelial FGFR signaling could be therapeutic for hypoxia-induced pulmonary hypertension by inhibiting the TGF-beta pathway-mediated EndMT process.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Endocrinology & Metabolism
Stephen Stone, Daniel J. Wegner, Jennifer A. Wambach, F. Sessions Cole, Fumihiko Urano, David M. Ornitz
JOURNAL OF THE ENDOCRINE SOCIETY
(2020)