Article
Microbiology
Tatsuya Nakayama, Hien Le Thi, Phong Ngo Thanh, Doan Tran Nguyen Minh, Oanh Nguyen Hoang, Phuong Hoang Hoai, Takahiro Yamaguchi, Michio Jinnai, Phuc Nguyen Do, Chinh Dang Van, Yuko Kumeda, Atsushi Hase
Summary: This study investigated the prevalence of antibiotic-resistant Escherichia coli in chicken meat from Vietnam and Japan. The results showed that colistin-resistant E. coli was found in approximately half of the chicken samples in Vietnam, with most of them harboring the mcr-1 gene. The prevalence of ESBL-producing E. coli remained constant in the past five years, with bla(CTX-M-55) or bla(CTX-M-65) being the predominant ESBL-related genes in Vietnam.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Wan-Ling Chen, Ming-Fang Cheng, Fan-Chen Tseng, Pin-Chien Wu, I-Fei Huang, Yun-Wen Chen, Yee-Hsuan Chiou, Yao-Shen Chen, Susan Shin-Jung Lee, Wan-Yu Hung, Jiun-Ling Wang, Chih-Hsin Hung
Summary: In this study, the distribution and resistance mechanisms of ESBL-producing E. coli strains were investigated in 120 children. The analysis revealed that these strains were mainly found in densely populated urban areas, with ST131 being the most challenging group. Therefore, the importance of enhancing environmental surveillance and sanitary procedures is emphasized.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Immunology
Zhen-Yu Wang, Yue Jiang, Yi-Qiao Shao, Heng-Fan Lu, Meng-Jun Lu, Xinan Jiao, Jing Wang, Qiu-Chun Li
Summary: This study investigates the prevalence and dissemination mechanism of ESBL-producing E. coli from healthy individuals in a community in Yangzhou, China. The study found nasal colonization of CTX-M-55-producing E. coli strains among healthy individuals in the community, indicating a potential risk of antimicrobial resistance dissemination through close contact or environmental transmission.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Khanzad Khudhur Jarjees, Rozhhalat Khudhur Jarjees, G. M. Qader
Summary: Pseudomonas aeruginosa is a common opportunistic pathogen with drug resistance potentially related to the production of broad spectrum beta lactamase enzymes. This study aimed to detect drug resistant strains and determine the frequency of extended spectrum beta lactamase enzymes in Pseudomonas aeruginosa. The findings highlight the increasing prevalence of multidrug resistant Pseudomonas aeruginosa strains with potential clinical implications.
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Microbiology
Andrew Cameron, Rupinder Mangat, Heba H. Mostafa, Samantha Taffner, Jun Wang, Ghinwa Dumyati, Richard A. Stanton, Jonathan B. Daniels, Davina Campbell, Joseph D. Lutgring, Nicole D. Pecora
Summary: Infections caused by ESBL-producing Escherichia coli are a significant health issue globally, with ST131 being the predominant clonal type. Besides bla(CTX-M-15), other ESBL variants may also be prevalent in certain regions. The identification of ST38 ESBL-producing E. coli in different regions of the United States, carrying bla(CTX-M-27) on distinct plasmid types, suggests the potential emergence of new ESBL lineages.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Yan Zhang, Yin-Huan Sun, Jiang-Yang Wang, Man-Xia Chang, Qiu-Yun Zhao, Hong-Xia Jiang
Summary: The study elucidated the prevalence of bla(CTX-M-27)-producing Escherichia coli and transmission mechanisms in swine farms in China. The main mechanism of transmission was through plasmids, with a novel structure identified that increased the stability of the bla(CTX-M-27) gene. Over time, the predominant vehicle for bla(CTX-M-27) transmission has diversified.
Article
Infectious Diseases
Julio A. Benavides, Marilia Salgado-Caxito, Andres Opazo-Capurro, Paulina Gonzalez Munoz, Ana Pineiro, Macarena Otto Medina, Lina Rivas, Jose Munita, Javier Millan
Summary: The study revealed the potential transmission of ESBL-E. coli among livestock, dogs, and wildlife worldwide, with findings in small-scale farms in Chile. Dogs were identified as potential reservoirs of the bacteria, highlighting the importance of further investigation in agricultural settings.
Article
Agriculture, Multidisciplinary
Wang Wen-jing, Wang Yi-fu, Jin Ya-jie, Song Wu-qiang, Lin Jia-meng, Zhang Yan, Tong Xin-ru, Tu Jian, Li Rui-chao, Li Tao
Summary: An extensively drug-resistant (XDR) Escherichia coli strain 258E was identified in an anal swab sample from a chicken farm in Anhui Province, China. The strain 258E carries an IncN plasmid pEC258-3, which contains multiple resistance genes and is highly similar to a plasmid found in Klebsiella pneumoniae. Conjugation experiments confirm the horizontal transferability of plasmid pEC258-3, posing a significant potential threat in animals, community, and hospital settings.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Microbiology
Kinga Toth, Akos Toth, Katalin Kamotsay, Viktoria Nemeth, Dora Szabo
Summary: This study reveals that ST131 clone, particularly C2/H30Rx and C1-M27 subclades, is the dominant clone of ESBL-producing E. coli collected in a hospital in Hungary.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2022)
Article
Infectious Diseases
Naiyaphat Nittayasut, Jitrapa Yindee, Pongthai Boonkham, Teerapong Yata, Nipattra Suanpairintr, Pattrarat Chanchaithong
Summary: This study characterized the genetic features of ESC-resistant E. coli isolated from cats and dogs with urinary tract infections in Thailand, revealing multiple antimicrobial resistance genes and a high prevalence of multidrug-resistant phenotypes. Additionally, the presence of various high-risk ExPEC clones suggests potential clonal dissemination in these animal reservoirs.
Article
Microbiology
Jin-Tao Yang, Li-Juan Zhang, Yang Lu, Rong-Min Zhang, Hong-Xia Jiang
Summary: CTX-M-55 was first discovered in Thailand in 2004 and has become the most common subtype of CTX-M enzyme in animal-origin E. coli in China. The widespread dissemination of bla(CTX-M-55)-positive E. coli poses a growing public health problem. This study constructed a global genomic data set of bla(CTX-M-55)-positive E. coli and explored its epidemiology and potential impact using high-resolution bioinformatics methods.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Chenyang Cao, Qinya Niu, Jia Chen, Xuebin Xu, Huanjing Sheng, Shenghui Cui, Bin Liu, Baowei Yang
Summary: The study analyzed 292 clinical isolates of S. Enteritidis in Shanghai between 2006 and 2014, showing a high frequency of cefotaxime-resistant isolates with CTX-M-55 being the most prevalent genotype. The findings highlight the importance of monitoring CTX-M-55 and using cephalosporins, especially cefotaxime, cautiously in empirical treatment for Salmonella infections.
Article
Agriculture, Dairy & Animal Science
Rajib Deb, Parul Chaudhary, Sachinandan De
Summary: CTX-M beta-lactamases are important resistance enzymes in E. coli, and a high prevalence of ESBL-positive E. coli isolates was found in mastitis cow milk. The resistance rates to various beta-lactam antibiotics were also high, and targeting the (CTX)-C-bla-M gene using CRISPR/Cas9 technology revealed changes in antibiotic susceptibility.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Microbiology
Chao Li, Renqiao Wen, Rongrong Mu, Xuan Chen, Peng Ma, Kui Gu, Zheren Huang, Zijing Ju, Changwei Lei, Yizhi Tang, Hongning Wang
Summary: The prevalence of CTX-M-55 type extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae is increasing worldwide, posing a threat to public and livestock health. This study demonstrates the horizontal transmission of bla(CTX-M-55) through outer membrane vesicles (OMVs) in avian pathogenic Escherichia coli (APEC). These findings contribute to a better understanding of the mechanisms by which antibiotics promote and disseminate resistance in the poultry industry, supporting the need for restrictions on antibiotic use in poultry.
Article
Microbiology
Mianzhi Yao, Qianhui Zhu, Jin Zou, Abebe Mekuria Shenkutie, Songnian Hu, Jiuxin Qu, Zilong He, Polly H. M. Leung
Summary: In this study, the genome assembly and characterization of a uropathogenic E. coli ST405 strain, SZESBLEC201, were reported. The strain carries three plasmids, with two of them harboring antibiotic resistance genes. Only one plasmid can be transferred via conjugation. The unique virulence pattern of SZESBLEC201 compared to genetically similar strains and the global spread of antibiotic resistance genes were highlighted.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Joachim Mertens, Jasmine Coppens, Katherine Loens, Marie Le Mercier, Basil Britto Xavier, Christine Lammens, Sarah Vandamme, Hilde Jansens, Herman Goossens, Veerle Matheeussen
Summary: The study introduces a flexible testing algorithm for rapid detection of current and emerging SARS-CoV-2 variants of concern/interest, which can be easily adjusted based on the local endemicity of specific variants.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Microbiology
Sien De Koster, Juan Pablo Rodriguez Ruiz, Sahaya Glingston Rajakani, Christine Lammens, Youri Glupczynski, Herman Goossens, Basil Britto Xavier
Summary: This study analyzed the genetic relationship of Klebsiella pneumoniae ST101 strains isolated from hospital samples, environment, community, and livestock using whole genome sequencing. The results showed that hospital-associated strains had higher resistance scores than non-clinical isolates, and the livestock-associated strains exhibited lower pathogenicity potential. Genetic diversity was observed between hospital-associated and healthy individuals' strains, suggesting a low public health risk from livestock strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Liesbet Van Heirstraeten, Esra Ekinci, Mathias Smet, Matilda Berkell, Laura Willen, Jasmine Coppens, An Spiessens, Basil Britto Xavier, Christine Lammens, Jan Verhaegen, Pierre Van Damme, Herman Goossens, Philippe Beutels, Veerle Matheeussen, Stefanie Desmet, Heidi Theeten, Surbhi Malhotra-Kumar
Summary: This study monitored the presence of SARS-CoV-2 in nasopharyngeal samples from young children aged 6-30 months attending day-care centres in Belgium from May 2020 to February 2022. The results showed that SARS-CoV-2 carriage among DCC children was detected only after the emergence of Delta and Omicron variants, with 9 out of the 42 screened DCCs being affected. Limited transmission of SARS-CoV-2 was observed among young children in Belgian DCCs during the studied period, as only one DCC had two children testing positive for SARS-CoV-2 at the same sampling time point.
Article
Infectious Diseases
Gabriel Torrens, Thomas Ewout van der Schalk, Sara Cortes-Lara, Leen Timbermont, Ester del Barrio-Tofino, Basil Britto Xavier, Laura Zamorano, Christine Lammens, Omar Ali, Alexey Ruzin, Herman Goossens, Samir Kumar-Singh, Jan Kluytmans, Fleur Paling, R. Craig MacLean, Thilo Kohler, Carla Lopez-Causape, Surbhi Malhotra-Kumar, Antonio Oliver
Summary: This study investigated the susceptibility profiles and resistome of Pseudomonas aeruginosa isolates from European ICUs. The results revealed a high prevalence of resistance, except for colistin, with a wide intercountry variability determined by the dissemination of XDR high-risk clones.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Chemistry, Analytical
Olivier Mortele, Basil Britto Xavier, Christine Lammens, Surbhi Malhotra-Kumar, Philippe G. Jorens, Eveline Dirinck, Alexander L. N. van Nuijs, Nina Hermans
Summary: Obesity is associated with dysbiosis of the gut microbiome, which affects the microbiotic biotransformation of xenobiotics. Differences in gut microbiome composition, bacterial concentration, and metabolic activity were observed between lean and obese populations. The obese gut microbiome showed weaker biotransformation ability for chlorogenic acid, leading to its persistence in the body. The lean population produced a greater number of biotransformation products.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Immunology
Vincent Van Averbeke, Matilda Berkell, Mohamed Mysara, Juan Pablo Rodriguez-Ruiz, Basil Britto Xavier, Fien H. R. De Winter, Bart 's Jongers, Ravi Kumar Jairam, An Hotterbeekx, Herman Goossens, E. Suzanne Cohen, Surbhi Malhotra-Kumar, Samir Kumar-Singh
Summary: This study investigates the interaction between gut microbiota and host immunity, and finds that alterations or deficiencies in Th1/Th2 balance can lead to significant changes in gut microbiota composition and function.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Elvis Achondou Akomoneh, Jolein Gyonne Elise Laumen, Said Abdellati, Christophe Van Dijck, Thibau Vanbaelen, Xavier Basil Britto, Sheeba S. Manoharan-Basil, Chris Kenyon
Summary: This study investigated the inhibitory effect of oropharyngeal microbiota and Listerine mouthwash on the growth of Neisseria gonorrhoeae and Neisseria meningitidis. It found that certain bacteria had inhibitory activity against these pathogens, but daily use of Listerine did not decrease their prevalence.
Article
Biology
Qiang Lin, Basil Britto Xavier, Blaise T. F. Alako, Alex L. Mitchell, Sahaya Glingston Rajakani, Youri Glupczynski, Robert D. Finn, Guy Cochrane, Surbhi Malhotra-Kumar
Summary: An extensive metagenomic analysis reveals the global distribution of antimicrobial resistance genes in different biomes, highlighting the importance of host genera and biomes in the spread of these genes.
COMMUNICATIONS BIOLOGY
(2022)
Article
Infectious Diseases
Christophe Van Dijck, Jolein Gyonne Elise Laumen, Tessa de Block, Said Abdellati, Irith De Baetselier, Achilleas Tsoumanis, Surbhi Malhotra-Kumar, Sheeba Santhini Manoharan-Basil, Chris Kenyon, Basil Britto Xavier
Summary: The oropharyngeal resistome of MSM using PrEP is significantly different from the general population, with higher levels of antimicrobial resistance genes, particularly for cephalosporins, macrolides, and fluoroquinolones. This suggests a need for stewardship campaigns to reduce antibiotic consumption in high-risk populations and mitigate the risk of infection.
JOURNAL OF INFECTION
(2023)
Article
Microbiology
Sien De Koster, Moniek Ringenier, Basil Britto Xavier, Christine Lammens, Dieter De Coninck, Katrien De Bruyne, Klaas Mensaert, Marjolein Kluytmans-van den Bergh, Jan Kluytmans, Jeroen Dewulf, Herman Goossens
Summary: This study characterized and traced ESBL-producing and ciprofloxacin-resistant Escherichia coli in Belgian food-producing animals. Multiple sequence types, resistance genes, and virulence profiles were detected. Transmission pathways within and between farms were identified, highlighting the importance of plasmid transmission in the spread of resistance.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Julio Diaz Caballero, Rachel M. Wheatley, Natalia Kapel, Carla Lopez-Causape, Thomas van der Schalk, Angus Quinn, Liam P. Shaw, Lois Ogunlana, Claudia Recanatini, Basil Britto Xavier, Leen Timbermont, Jan Kluytmans, Alexey Ruzin, Mark Esser, Surbhi Malhotra-Kumar, Antonio Oliver, R. Craig MacLean
Summary: Here, the authors extensively characterized patients colonized with different strains of Pseudomonas aeruginosa to study the impact of within-host diversity on the development of antibiotic resistance. They found that mixed strain populations are common in this pathogen, and resistance rapidly evolves through selection for pre-existing resistant strains in patients colonized with multiple strains. In contrast, resistance sporadically evolves in patients colonized with a single strain due to selection for novel resistance mutations. Furthermore, the study suggests that within-host diversity can also lead to the loss of resistance in the absence of antibiotic treatment.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Chris Kenyon, Zina Gestels, Thibaut Vanbaelen, Said Abdellati, Dorien van den Bossche, Irith De Baetselier, Basil Britto Xavier, Sheeba Santhini Manoharan-Basil
Summary: This study evaluated the impact of doxycycline PEP on resistance in Klebsiella pneumoniae and found that it can select for resistance to doxycycline, ceftriaxone, and ciprofloxacin in a G. mellonella model. The emergence of ramR mutations in K. pneumoniae was similar to those seen in circulating strains. These findings suggest the need for further assessment of the effect of doxycycline PEP on resistance induction in a broader range of bacterial species.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Sahaya Glingston Rajakani, Basil Britto Xavier, Adwoa Sey, El Bounja Mariem, Christine Lammens, Herman Goossens, Youri Glupczynski, Surbhi Malhotra-Kumar
Summary: This study utilizes population analysis profiling (PAP) and time-kill assay for the detection of heteroresistance in K. pneumoniae and for evaluating the association between in vitro regrowth and heteroresistance. The mechanisms of colistin resistance and the ability of combination therapies to suppress resistance selection were also analysed.
Article
Critical Care Medicine
Tjitske S. R. van Engelen, Tom D. Y. Reijnders, Fleur Paling, Marc J. M. Bonten, Leen Timbermont, Surbhi Malhotra-Kumar, Jan A. J. W. Kluytmans, Hessel Peters-Sengers, Tom van der Poll
Summary: This study confirmed the association between immune suppression and the occurrence of pneumonia in critically ill patients. By analyzing plasma protein biomarkers in patients who developed ICU-acquired pneumonia and those who did not, the study found that there were broad host immune aberrations in the trajectory to pneumonia. The results showed that patients who developed ICU-acquired pneumonia displayed enhanced inflammation, disturbed endothelial barrier function, and abnormal coagulation responses.
Article
Cell Biology
Lize Cuypers, Els Keyaerts, Samuel Leandro Hong, Sarah Gorissen, Soraya Maria Menezes, Marick Starick, Jan Van Elslande, Matthias Weemaes, Tony Wawina-Bokalanga, Joan Marti-Carreras, Bert Vanmechelen, Bram Van Holm, Mandy Bloemen, Jean-Michel Dogne, Francois Dufrasne, Keith Durkin, Jean Ruelle, Ricardo De Mendonca, Elke Wollants, Pieter Vermeersch, Caroline Boulouffe, Achille Djiena, Caroline Broucke, Boudewijn Catry, Katrien Lagrou, Marc Van Ranst, Johan Neyts, Guy Baele, Piet Maes, Emmanuel Andre, Simon Dellicour, Johan Van Weyenbergh