Article
Chemistry, Analytical
Reza Lotfi Khatoonabadi, Maryam Vosough, Lotta L. Hohrenk, Torsten C. Schmidt
Summary: This study utilized chemometrics-based methods to analyze pollution patterns in river water samples affected by wastewater treatment plants, demonstrating the importance of non-target screening and multivariate curve resolution techniques in improving our understanding of co-occurring pollutants in the environment and trends of contaminants of emerging concern (CECs) in water bodies.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Andrea Cerrato, Carmen Bedia, Anna Laura Capriotti, Chiara Cavaliere, Vincenzo Gentile, Martina Maggi, Carmela Maria Montone, Susy Piovesana, Alessandro Sciarra, Roma Tauler, Aldo Lagan
Summary: The study focused on analyzing polar and positively charged metabolites in urine samples from benign prostatic hyperplasia and prostatic cancer patients, utilizing an analytical platform with up to 40-fold analyte enrichment. By applying the ROIMCR procedure to complex metabolomics datasets generated by UHPLC-HRMS, differential metabolites were successfully resolved and tentatively identified, underscoring the importance of this approach in cancer biomarker research. The study highlights the significance of previously neglected metabolite classes in cancer biomarker discovery and the value of the proposed methodology.
ANALYTICA CHIMICA ACTA
(2021)
Article
Oncology
Caridad Diaz, Carmen Gonzalez-Olmedo, Leticia Diaz-Beltran, Jose Camacho, Patricia Mena Garcia, Ariadna Martin-Blazquez, Monica Fernandez-Navarro, Ana Laura Ortega-Granados, Fernando Galvez-Montosa, Juan Antonio Marchal, Francisca Vicente, Jose Perez del Palacio, Pedro Sanchez-Rovira
Summary: The outcomes of neoadjuvant chemotherapy in breast cancer vary according to subtype. This study used metabolomics to identify predictors of treatment response in different breast cancer subtypes. Potential biomarker candidates were identified for the triple-negative subtype, while significant differences were observed in the luminal B and HER2+ subtypes. The combination of metabolomics and statistical approaches may improve personalized treatment and follow-up in breast cancer.
MOLECULAR ONCOLOGY
(2022)
Article
Evolutionary Biology
Vid Svara, Stefan G. Michalski, Martin Krauss, Tobias Schulze, Stephan Geuchen, Werner Brack, Till Luckenbach
Summary: Anthropogenic chemicals in freshwater environments can lead to ecosystem degradation and biodiversity decline. This study investigated the impact of anthropogenic organic micropollutants (AOM) on the genetic diversity of the common amphipod Gammarus pulex in central Germany. It was found that AOM levels were associated with changes in genetic diversity of G. pulex.
EVOLUTIONARY APPLICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Shivani Singh Gaur, Uday S. Annapure
Summary: This report discusses the metabolites produced by Enterococcus strain E.villorum SB2, isolated from vaginal source. The bacteria were grown in different media and M17 medium was selected for metabolite production. HR-LCMS analysis revealed the production of various metabolites, including amino acids, organic acids, peptides, flavonoids, terpenoids, indole derivatives, antimicrobial compounds, and fatty acids. These findings open up new possibilities for industrial applications of the strain.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Xinxin Lai, Jinhai Liu, Wenyuan Li, Min Qiao, Mingheng Qiu, Lingpan Lu
Summary: This study comprehensively reports the in vitro metabolism of remibrutinib, identifying and characterizing 20 metabolites and highlighting the major metabolic changes. These findings are critical for the understanding of remibrutinib metabolism and safety evaluation.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Chemistry, Analytical
Sarmento J. Mazivila, Juan M. Lombardi, Ricardo N. M. J. Pascoa, Santiago A. Bortolato, Joao M. M. Leitao, Joaquim C. G. Esteves da Silva
Summary: The study demonstrates the use of FAPV algorithm as a proper method for aligning second-order chromatographic data with severe retention time shifts, showing successful decomposition by PARAFAC and MCR-ALS models. The results indicate that FAPV algorithm effectively restores trilinearity lost due to shifted and warped elution time profiles, leading to accurate trilinear decomposition with low relative prediction error values.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biology
Muzaffer Mutlu, Zeynebe Bingol, Eda Mehtap Uc, Ekrem Koksal, Ahmet C. Goren, Saleh H. Alwasel, Ilhami Gulcin
Summary: This study evaluated the antioxidant and antidiabetic properties of cinnamon leaf essential oil and determined its inhibitory effects on enzymes associated with metabolic diseases. Phenolic contents and essential oil profiles were analyzed. Cinnamon oil showed strong radical scavenging abilities and effectively inhibited carbonic anhydrase II, acetylcholinesterase, and alpha-amylase. This multidisciplinary study is valuable for further research on antioxidant properties and enzyme inhibition profiles of important plants and their oils.
Article
Biology
Eda Mehtap Ozden, Zeynebe Bingol, Muzaffer Mutlu, Hasan Karagecili, Ekrem Koksal, Ahmet C. Goren, Saleh H. Alwasel, Ilhami Gulcin
Summary: This research comprehensively examined and researched the kiwifruit oil using various bioanalytical methods, determining its antioxidant, antiglaucoma, antidiabetic, and anti-Alzheimer's capabilities. The inhibitory effects on several enzymes linked to metabolic illnesses were also established. The chemical composition and quantity of essential oils contained in kiwifruit oil were studied. The results demonstrated the potent radical scavenging properties and inhibition effects on enzymes of kiwifruit oil, suggesting its significant potential in pharmaceutical procedures.
Article
Pharmacology & Pharmacy
Yiqing Guo, Huanhuan Lv, Jing Lv, Zenghong Jiang
Summary: The in vitro metabolism of hirsutine was investigated using liver microsomes and human recombinant cytochrome P450 enzymes, and it was found that hirsutine was mainly metabolized by CYP3A4. The study provided valuable insights into the metabolic fate of hirsutine in liver microsomes and its potential hepatotoxicity.
Article
Biochemistry & Molecular Biology
Mary T. Doan, Michael D. Neinast, Erika L. Varner, Kenneth C. Bedi, David Bartee, Helen Jiang, Sophie Trefely, Peining Xu, Jay P. Singh, Cholsoon Jang, J. Eduardo Rame, Donita C. Brady, Jordan L. Meier, Kenneth B. Marguiles, Zoltan Arany, Nathaniel W. Snyder
Summary: Research has shown that mammals can synthesize a six-carbon compound through the metabolism of propionate, and this reaction has been confirmed in humans and mice.
JOURNAL OF LIPID RESEARCH
(2022)
Article
Endocrinology & Metabolism
Umaporn Uawisetwathana, Siwat Plaisen, Sopacha Arayamethakorn, Prapatsorn Jitthiang, Wanilada Rungrassamee
Summary: The study aimed to develop extraction and analytical methods for accurate metabolic analysis in shrimp intestine. The optimized conditions included using ethyl acetate:acetone extraction, acetonitrile:water elution, and mass fragmentation at 15% HCD. This method enabled in-depth study of shrimp-microbial interaction at the metabolite level.
Article
Chemistry, Medicinal
Annelies Heylen, Yannick Vermeiren, Ido P. Kema, Martijn van Faassen, Claude van der Ley, Debby Van Dam, Peter P. De Deyn
Summary: Despite distinct clinical profiles, ALS and FTD patients share pathological features, with a substantial percentage of patients displaying a mixed disease phenotype. Kynurenine metabolism plays a role in dementia-associated neuroinflammation and has been linked to both diseases. Kynurenine pathway metabolite levels were significantly lower in ALS patients compared to FTD, EOAD, and control subjects in multiple brain regions. This suggests that the contribution of kynurenine metabolism in neuroinflammation is lower in ALS than in FTD or EOAD, possibly due to differences in age of onset.
Article
Biochemical Research Methods
Stephane Trevisiol, Yves Moulard, Zied Kaabia, Vivian Delcourt, Benoit Loup, Patrice Garcia, Sophie Boyer, Karine Dauriac, Guillaume Groseille, Sebastien Rouger, Ruediger Narbe, Marie-Agnes Popot, Ludovic Bailly-Chouriberry
Summary: Ciclesonide is a highly potent inhaled corticosteroid used for the treatment of asthma in humans and horses. Due to its anti-inflammatory properties, it is prohibited for use in horse competitions, necessitating the development of sensitive analytical methods for detection in plasma.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Sara Malaca, Marilyn A. Huestis, Leonardo Lattanzio, Luigi T. Marsella, Adriano Tagliabracci, Jeremy Carlier, Francesco P. Busardo
Summary: This study identified the optimal metabolites for detecting 4-AcO-DiPT consumption through human hepatocyte metabolism and high-resolution mass spectrometry. The metabolites 4-OH-DiPT, 4-OH-iPT, and 4-OH-DiPT-N-oxide were suggested as biomarkers for identifying 4-AcO-DiPT use. These findings are crucial for investigations into tryptamine intoxications and fatalities.
Article
Oncology
Bahare Shokouhian, Babak Negahdari, Zahra Heydari, Mehdi Totonchi, Hamidreza Aboulkheyr Es, Abbas Piryaei, Ebrahim Mostafavi, Massoud Vosough
Summary: This study identifies HNF4α as a key protein in hepatocellular carcinoma (HCC) that is associated with epithelial-mesenchymal transition (EMT) and reprogrammed cancer metabolism. Increasing HNF4α expression through synthetic antisense long non-coding RNA improves HCC cell function, slows down glycolysis, accelerates gluconeogenesis, improves dysregulated cholesterol metabolism, and inhibits migration, invasion, and proliferation of HCC cells.
Review
Genetics & Heredity
Zahra Heydari, Maria Peshkova, Zeynep Burcin Gonen, Ianos Coretchi, Ahmet Eken, Arzu Hanim Yay, Muhammet Ensar Dogan, Nuriye Gokce, Hilal Akalin, Nastasia Kosheleva, Daniela Galea-Abdusa, Mariana Ulinici, Valentina Vorojbit, Anastasia Shpichka, Stanislav Groppa, Massoud Vosough, Mihail Todiras, Denis Butnaru, Yusuf Ozkul, Peter Timashev
Summary: Extracellular vesicles (EVs) are produced by various cells and play a crucial role in cell-cell signaling, immune response, and tumor metastasis. They can deliver functional biomolecules and have the potential for therapeutic and diagnostic applications.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Chemistry, Applied
Maryam Mousavizadegan, Morteza Hosseini, Mahsa N. Sheikholeslami, Yalda Hamidipanah, Mohammad Reza Ganjali
Summary: This study presents a rapid detection method using machine learning algorithms for the analysis of tetracycline in water and milk samples. The developed models achieved high accuracy in identifying the presence of tetracycline, demonstrating the potential of machine learning in the development of rapid sensors for food analytes.
Review
Cell Biology
Kasra Moeinabadi-Bidgoli, Malihe Rezaee, Nikoo Hossein-Khannazer, Amirhesam Babajani, Hamid Asadzadeh Aghdaei, Mandana Kazem Arki, Siamak Afaghi, Hassan Niknejad, Massoud Vosough
Summary: Ischaemic disorders are a significant global health burden that cannot be fully cured by current therapeutic approaches. Exosomes, with their multiple bioactive components, are considered as cell-free therapeutics and have the potential to be an ideal complementary therapeutic tool for ischemic disorders. Their convenience in storage and utilization, as well as their lack of cell therapy concerns, make them a promising approach for angiogenesis induction and other therapeutic functions in ischemic disorders.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Cell & Tissue Engineering
Bahareh Sadri, Mohammad Hassanzadeh, Abolfazl Bagherifard, Javad Mohammadi, Mehdi Alikhani, Kasra Moeinabadi-Bidgoli, Hoda Madani, Dylana Diaz-Solano, Shahedeh Karimi, Mohammad Mehrazmay, Mehdi Mohammadpour, Massoud Vosough
Summary: This study aimed to evaluate the therapeutic effect of adipose-derived mesenchymal stromal cells (AD-MSCs) in knee osteoarthritis (KOA). The results showed that the thickness of articular cartilage increased in patients treated with AD-MSCs, and there was a significant improvement in clinical evaluation.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Environmental Sciences
Maria Paula Carrillo, Marina Sevilla, Marta Casado, Benjamin Pin, Victor Matamoros, Maria Vila-Costa, Carlos Barata
Summary: Wastewater Treatment Plant (WWTP) effluents are significant sources of antibiotics, antibiotic resistance genes (ARGs), and resistant bacteria, posing threats to aquatic biota and human health. This study explores the impact of the antibiotic doxycycline on the microbiome of Daphnia magna in lab water and treated wastewater media. The results indicate that doxycycline and the media significantly influence the composition of the Daphnia-associated microbiome, with notable changes in Enterococcus spp. Furthermore, doxycycline reduces the presence of Limnohabitans sp. and impairs Daphnia reproduction in lab water, while treated wastewater increases microbiome diversity, richness, and promotes fecundity. The study also detects various ARG genes, including qnrS1, sul1, and blaTEM, with a higher abundance in treated wastewater compared to lab water.
ENVIRONMENTAL POLLUTION
(2023)
Article
Cell Biology
Mahtab Shahriari Felordi, Mehdi Alikhani, Zahra Farzaneh, Mahmoud Alipour Choshali, Marzieh Ebrahimi, Hamidreza Aboulkheyr Es, Abbas Piryaei, Mustapha Najimi, Massoud Vosough
Summary: The anti-cancer properties of EGCG involve induction of apoptosis, inhibition of cell proliferation and histone deacetylase. This study examined the role of EGCG in gastric cancer cells by dissociating the c-FLIP/Ku70 complex and found that EGCG induced apoptosis, changed the expression of various proteins involved in cell cycle regulation, and decreased the interaction between c-FLIP and Ku70 while increasing Ku70 nuclear content. By dissociating the c-FLIP/Ku70 complex, EGCG could improve the therapeutic outcome in combination with other cancer therapy protocols.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Medicine, Research & Experimental
Samaneh Siapoush, Ramazan Rezaei, Helia Alavifard, Behzad Hatami, Mohammad Reza Zali, Massoud Vosough, Shahrokh Lorzadeh, Marek J. Los, Kaveh Baghaei, Saeid Ghavami
Summary: Liver fibrosis is characterized by excessive deposition and accumulation of extracellular matrix components, mainly collagens, in response to various triggers. Autophagy, a homeostatic system for cell survival, and transforming growth factor-beta 1 (TGF-β1) play important roles in liver fibrosis. Recent studies have shown that TGF-β1 regulates autophagy, which affects pathophysiological aspects of liver fibrosis. This review highlights the cellular and molecular mechanisms of autophagy, its regulation by TGF-β1, and the potential for simultaneous inhibition of these pathways in anti-fibrotic therapy.
Article
Endocrinology & Metabolism
Jamile Mohammad Jafari, Josefina Casas, Carlos Barata, Hamid Abdollahi, Roma Tauler
Summary: This study investigated the temporal disruptive effects of TBT on lipid homeostasis in Daphnia magna using LC-MS analysis. The data sets were analyzed using bilinear and trilinear non-negative factor decomposition chemometric methods, which allowed for the identification of specific patterns and provided insight into the effects of TBT on lipid homeostasis in Daphnia magna.
Article
Cell Biology
Amir Bajouri, Dorsa Dayani, Anoushe Taj Sharghi, Shahedeh Karimi, Maryam Niknejadi, Kasra Moeinabadi Bidgoli, Hoda Madani, Fatemeh Abbasi Kakroodi, Tina Blourieh, Sura Mardpour, Neda Jaroughi, Nasser Aghdami, Leila Ataie-Fashtami, Saeid Shafiyan, Massoud Vosough
Summary: The safety and potential efficacy of injecting allogeneic adipose-derived mesenchymal stromal cells (ADSCs) in psoriatic patients were evaluated in this study. The results showed that after ADSCs injection, the lesions of the patients improved and no major adverse effects were observed. Additionally, the expression levels of pro-inflammatory factors in the patient blood samples were reduced, indicating modulation of inflammation after ADSCs administration. Overall, ADSC injection could be considered as a safe and effective therapeutic approach for psoriatic plaques.
Article
Cell Biology
Melika Zangeneh Motlagh, Nazanin Mahdavi, Zohreh Miri-Lavasani, Pouyan Aminishakib, Mahsa Khoramipour, Mandana Kazem Arki, Niloufar Rezaei, Nikoo Hossein-Khannazer, Massoud Vosough
Summary: The study aimed to assess the effectiveness of disulfiram (DSF)-loaded Niosomes on cancerous phenotypes of the OSCC cells.
Review
Cell Biology
Maryam Sadat Hosseini, Masoumeh Nouri, Morteza Zarrabi, Mohammad Javad Fatemi, Anastasia Shpichka, Peter Timashev, Moustapha Hassan, Massoud Vosough
Summary: The contribution of platelets in tissue regeneration and their potential application in regenerative medicine has been well established. Platelet rich plasma (PRP) with higher concentrations of platelets is now a promising therapy for tissue repair and regeneration in various medical fields. Burn injuries are devastating trauma with long-term medical care and high costs. Post-burn scars are inevitable, making the development of new treatment modalities necessary. This review aims to provide insight into the use of PRP as an adjuvant therapy for burn injuries and subsequent scars.
Review
Cell Biology
Shahriar Ghashghaei, Mahkameh Abbaszadeh, Shahedeh Karimi, Leila Ataie-Fashtami, Amir Bajouri, Massoud Vosough
Summary: Vitiligo is an autoimmune disease that affects 0.2-1.8% of the global population, causing depigmentation of the skin. Current treatments like topical corticosteroids and calcineurin inhibitors have side effects, while phototherapies are limited in effectiveness. Advanced therapy medicinal products (ATMPs), such as non-cultured epidermal cells and melanocytes, have shown promise in treating vitiligo, but affordability remains a challenge.
Review
Biology
Negar Aasadollahei, Niloufar Rezaei, Reihaneh Golroo, Tarun Agarwal, Massoud Vosough, Abbas Piryaei
Summary: Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease worldwide. However, the lack of reliable in vitro models has limited drug development studies, resulting in no approved medicine for NAFLD treatment. A biomimetic in vitro liver model requires an optimal natural microenvironment with appropriate cellular composition and specific bio-molecules to mimic native tissue conditions. Bioengineered three-dimensional tissues, such as microtissues and organoids, and infusion-based cultivation systems like microfluidics can improve the physiological function of in vitro constructs.
Article
Environmental Sciences
Pol Dominguez-Garcia, Reinerio Rolando Rodriguez, Carlos Barata, Cristian Gomez-Canela
Summary: SARS-CoV-2 causes the COVID-19 pandemic, which has been declared a global public health emergency and resulted in millions of deaths. The increased global pharmaceutical consumption due to the pandemic may pose an environmental threat. Analytical techniques are necessary to detect pharmaceutical residues in aquatic environments.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
