Article
Biotechnology & Applied Microbiology
Xianggang Wang, Zhengyan Li, Zhonghan Wang, He Liu, Yutao Cui, Yuzhe Liu, Ming Ren, Hongsheng Zhan, Zuhao Li, Minfei Wu, Jincheng Wang
Summary: The study developed a thermosensitive hydrogel loaded with BMP-2 and OPG to promote bone formation and inhibit osteoclast activity, which was then used to construct 3D-printed composite scaffolds. These scaffolds facilitated osteogenic differentiation, reduced osteoclastic activation, and improved bone ingrowth and osseointegration through sustained release of BMP-2 and OPG.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Yi Sui, Aierpati Yusufu, Kaiwei Nian, Xin Li, Wenhua Shi, Bo Cheng, Bin Shen
Summary: A BMP-2@CNT delivery system was constructed for the treatment of osteoporosis, and the feasibility of a nanodrug delivery system was explored. The results showed that nano-BMP-2 effectively promoted osteoblast proliferation and improved OP symptoms, with good stability and sustained release.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Claire I. A. van Houdt, Marianne K. E. Koolen, Paula M. Lopez-Perez, Dietmar J. O. Ulrich, John A. Jansen, Sander C. G. Leeuwenburgh, Harrie H. Weinans, Jeroen J. J. P. van den Beucken
Summary: The study found that self-healing hydrogels based on calcium phosphate nanoparticles and bisphosphonate conjugated hyaluronan alone cannot heal critical size segmental femoral bone defects in rats, but loading with low dose BMP-2 significantly improves bone formation. On the other hand, high dose BMP-2 loading does not enhance bone formation within the defect area, but leads to excessive bone formation outside the defect area.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Multidisciplinary Sciences
John W. Kennedy, P. Monica Tsimbouri, Paul Campsie, Shatakshi Sood, Peter G. Childs, Stuart Reid, Peter S. Young, Dominic R. M. Meek, Carl S. Goodyear, Matthew J. Dalby
Summary: In this study, nanovibrational stimulation was used to reduce osteoclast formation and activity in human mononuclear CD14(+) blood cells, enhance osteogenesis, and reduce osteoclastogenesis in a co-culture of primary human bone marrow stromal cells and bone marrow hematopoietic cells. Akt was identified as a potential mediator, known to be involved in bone differentiation and reduced osteoclast activity. The nanovibrational bioreactor has potential to provide humanized 3D models for drug screening after further validation.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Magdalena Wytrwal, Malgorzata Sekula-Stryjewska, Agata Pomorska, Ewa Oclon, Ewa Zuba-Surma, Szczepan Zapotoczny, Krzysztof Szczubialka
Summary: This study investigated the covalent binding of recombinant human bone morphogenetic protein-2 and -7 in stem cells and its effects on cell adhesion, migration, and osteogenesis. The presence of both proteins enhanced cell adhesion but limited cell motility, while the presence of single proteins promoted cell migration activity. Additionally, the coexistence of the proteins significantly increased early osteogenic marker expression.
Article
Engineering, Biomedical
Sarah A. Schoonraad, Alan A. Jaimes, Arjun J. X. Singh, Kiera J. Croland, Stephanie J. Bryant
Summary: This study investigates the osteogenic effects of tethered BMP-2 and BMP-9 on human MSCs encapsulated in a hydrogel. The results show that tethered BMP-2 and BMP-9 both effectively enhance osteogenesis of MSCs, leading to a mature osteoblast phenotype.
ACTA BIOMATERIALIA
(2023)
Review
Cell Biology
Kazuki Inoue, Courtney Ng, Yuhan Xia, Baohong Zhao
Summary: The translation discusses the role of osteoclasts in bone development and remodeling, as well as the significance of epigenetic regulation and microRNAs in gene expression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Biao Wu, Jie Shang, Shiyuan Lin, Ning Jiang, Baizhou Xing, Rong Peng, Xianghe Xu, Huading Lu
Summary: Increased bone resorption caused by excessive osteoclasts is the main cause of osteoporosis. The expression of RILP, a protein, is induced by receptor activator of NF-KB ligand, and its inhibition decreases the number and function of osteoclasts. Inhibition of RILP also reduces preosteoclast migration and bone resorption. The study suggests that RILP plays a crucial role in osteoclast formation and bone resorption, and could be a therapeutic target for bone diseases caused by excessive osteoclasts.
LABORATORY INVESTIGATION
(2023)
Article
Biotechnology & Applied Microbiology
Rongkui Zhou, Shichang Miao, Jun Xu, Liping Sun, Yaofei Chen
Summary: The study showed that Circ_0000020 is upregulated during osteogenic differentiation and interacts with miR-142-5p to regulate BMP2 expression, promoting osteogenic differentiation of BMSCs. This positive regulation occurs through the Circ_0000020/miR-142-5p/BMP2/SMAD-dependent signaling pathway.
Article
Biochemistry & Molecular Biology
Honglei Kang, Qian Guo, Yimin Dong, Renpeng Peng, Kehan Song, Jia Wang, Haiyang Liu, Meipeng Zhu, Hongjian Zhao, Hanfeng Guan, Feng Li
Summary: MAT2A is crucial for osteoclastogenesis and may be a potential therapeutic target for the treatment of osteoporosis.
Article
Materials Science, Biomaterials
M. C. Echave, I Erezuma, N. Golafshan, M. Castilho, F. B. Kadumudi, C. Pimenta-Lopes, F. Ventura, A. Pujol, J. J. Jimenez, J. A. Camara, R. Hernaez-Moya, L. Iturriaga, L. Saenz Del Burgo, I. Iloro, M. Azkargorta, F. Elortza, R. Lakshminarayanan, T. H. Al-Tel, P. Garcia-Garcia, R. Reyes, A. Delgado, C. Evora, J. L. Pedraz, A. Dolatshahi-Pirouz, G. Orive
Summary: In this study, a new osteoconductive scaffold was developed, which reduced the required doses of BMP-2 for bone regeneration. The scaffold showed promising results in promoting cell adhesion, proliferation, and osteogenic differentiation of human bone-marrow derived mesenchymal stem cells (hBM-MSCs). The sustained release of BMP-2 from the scaffold allowed for the treatment of critical-sized osteoporotic bone defects with relatively low growth factor doses.
BIOMATERIALS ADVANCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hyun Ju Kim, Kyoung-Hwa Kim, Yong-Moo Lee, Young Ku, In-Chul Rhyu, Yang-Jo Seol
Summary: The use of rhBMP-2/collagen gel significantly enhanced alveolar bone regeneration compared to rhPDGF-BB/collagen gel in OVX-induced osteoporotic rats. Micro-CT analysis showed that the BMP-2 group had the highest bone volume fraction, while the PDGF-BB group did not show significant differences compared to the collagen group. Histomorphometric analysis revealed that the BMP-2 group had a significantly larger amount of new bone area compared to the control and collagen groups at 4 weeks, indicating its superiority in promoting bone healing.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Biochemistry & Molecular Biology
Hyun Seok, Hee-Youl Kim, Dong-Cheol Kang, Jung-Ho Park, Jong Hoon Park
Summary: The study compared the bone regeneration ability of bone scaffolds using particle and block bones with rhBMP-2, showing that rhBMP-2 with block bones resulted in significantly higher bone volume but also led to the formation of adipose tissue and void spaces.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Chengcheng Zhang, Jingru Qin, Lu Yang, Zhiyao Zhu, Jia Yang, Wan Su, Haibin Deng, Zhongqi Wang
Summary: This study reveals that exosomes secreted from pulmonary adenocarcinoma cells enhance osteoclastogenesis and osseous resorption by downregulating neuropilin 2 expression. These findings shed light on the mechanism of osseous metastases in pulmonary carcinoma and provide a potential nanodrug for treating bone metastases.
CELL DEATH DISCOVERY
(2022)
Article
Pharmacology & Pharmacy
Hao Tian, Tao Jiang, Kai Yang, Ruonan Ning, Tianqi Wang, Qi Zhou, Niandong Qian, Ping Huang, Lei Guo, Min Jiang, Xiaobing Xi, Xing Xu, Lianfu Deng
Summary: This study found that alpha-asarone (ASA), a compound derived from the traditional Chinese medicinal herb Acorus tatarinowii, can inhibit the activity of overactivated osteoclasts and reduce bone resorption. ASA's effects on osteoclastogenesis are dose-dependent and mediated through multiple signaling pathways. In an estrogen-deficiency-induced osteoporosis model, ASA also improved bone microstructure.
FRONTIERS IN PHARMACOLOGY
(2022)