Article
Oncology
Gabriele Greve, Geoffroy Andrieux, Pascal Schlosser, Nadja Blagitko-Dorfs, Usama-Ur Rehman, Tobias Ma, Dietmar Pfeifer, Gerhard Heil, Andreas Neubauer, Juergen Krauter, Michael Heuser, Helmut R. Salih, Konstanze Doehner, Hartmut Doehner, Bjorn Hackanson, Melanie Boerries, Michael Luebbert
Summary: This study investigates the mechanisms of action of DNA-hypomethylating agents (HMAs) in AML/MDS therapy. The results demonstrate non-random early in vivo demethylation events in AML blasts associated with gene induction. Additionally, a selective demethylation phenomenon was observed in malignant blasts compared to T-cells. Further studies with larger patient cohorts are needed to determine if a demethylation signature can predict treatment response.
Article
Toxicology
Hu Li, Xinru Mao, Kai Liu, Jiahao Sun, Benrui Li, Rahmani Mohammad Malyar, Dandan Liu, Cuiling Pan, Fang Gan, Yunhuan Liu, Kehe Huang, Xingxiang Chen
Summary: Ochratoxin A (OTA) can induce renal injury through NLRP3 inflammasome activation and caspase-1-dependent pyroptosis. Both in vivo and in vitro experiments demonstrated that OTA exposure leads to renal fibrosis and inflammation, which can be alleviated by inhibiting NLRP3 activation and pyroptosis.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Environmental Sciences
Kaihao Wang, Min Zhou, Yipeng Du, Peixin Li, Zheng Huang
Summary: This study investigates the effects of zearalenone on cardiovascular aging through in vitro and in vivo experiments. Results show that zearalenone treatment leads to increased cardiovascular aging-related damage, while zeaxanthin, a powerful antioxidant, can partially alleviate zearalenone-induced cardiovascular aging.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Genetics & Heredity
Do-Won Lee, Kyeong-Min Kim, Seulki Park, Sang-Hyun An, Young-Ju Lim, Won-Gu Jang
Summary: Eucalyptol (EU) promotes osteoblast differentiation and bone formation through ERK phosphorylation. It also has the potential to promote bone regeneration and prevent osteoporosis.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Cell Biology
Yan Li, Haonan Zhang, Xiaomin Li, Wanyu Wu, Ping Zhu
Summary: The organization and dynamics of chromatin fiber, regulated by linker histone, are crucial for gene expression. Cryoelectron tomography (cryo-ET), sub-volume averaging, and 3D segmentation techniques were used to visualize the folding of in vitro and in vivo chromatin fibers. The study found that changes in nucleosome repeat length and fiber length do not alter the two-start helical architecture in homogeneous reconstituted chromatin. Heterogeneous compositions were observed in isolated chromatin fibers, but they mainly affected local conformation rather than the overall architecture. Cryo-ET with voltage phase plate revealed zigzag DNA trajectories between alternate nucleosomes. These findings suggest a general folding mode of chromatin induced by linker histone and provide insights into the importance of local conformation in chromatin structure.
Article
Endocrinology & Metabolism
Francesco Andreozzi, Concetta Di Fatta, Rosangela Spiga, Gaia Chiara Mannino, Elettra Mancuso, Carolina Averta, Carmen De Caro, Martina Tallarico, Antonio Leo, Rita Citraro, Emilio Russo, Giovambattista De Sarro, Giorgio Sesti
Summary: The study found a positive relationship between fasting glucagon levels and various proinflammatory cytokines and acute-phase reactants. Through animal experiments and in vitro cell experiments, the researchers discovered that glucagon can stimulate the activation of proinflammatory pathways in the liver, which may be related to hyperglycemia and cardiometabolic risk.
DIABETES OBESITY & METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Peter G. Vaughan-Shaw, James P. Blackmur, Graeme Grimes, Li-Yin Ooi, Anna M. Ochocka-Fox, Karen Dunbar, Alex von Kriegsheim, Vidya Rajasekaran, Maria Timofeeva, Marion Walker, Victoria Svinti, Farhat V. N. Din, Susan M. Farrington, Malcolm G. Dunlop
Summary: In this study, patient derived organoids and CRC cell lines were used to demonstrate that calcitriol can increase the expression of the CRC tumor suppressor gene CDH1. Additionally, several other genes with established links to carcinogenesis showed differential expression after calcitriol treatment. The results suggest that calcitriol can modulate genes and pathways related to CRC tumorigenesis, providing a potential mechanism for the observed association between vitamin D and CRC.
Article
Environmental Sciences
Bo Qian, Rong-Juan Jiang, Jia-Le Song, Chen-Qiang Wang
Summary: TDCPP-induced ferroptosis may be associated with its neurotoxicity, potentially through the PINK1/PARKIN-mediated mitophagy initiated by mitochondrial damage.
Review
Environmental Sciences
Marcelo Souza Silva, Daniel Vitor De Souza, Maria Esther Suarez Alpire, Andrea Cristina De Moraes Malinverni, Regina Claudia Barbosa Da Silva, Milena De Barros Viana, Celina Tizuko Fujiyama Oshima, Daniel Araki Ribeiro
Summary: This study provides a mini review regarding genotoxicity induced by dimethoate as a result of oxidative stress. The research showed that multiple studies have been published in scientific literature, demonstrating the approach in various tissues and organs, but there are limited studies in humans. New studies are crucial for better understanding the pathobiological events of genotoxicity on human cells induced by dimethoate.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Food Science & Technology
Jinling Cui, Shutao Yin, Chong Zhao, Lihong Fan, Hongbo Hu
Summary: This study demonstrated that the co-exposure of patulin and cadmium resulted in synergistic cytotoxicity in liver or renal cells in vitro and enhanced liver toxicity in vivo. The synergistic toxicity was attributed to boosted ROS generation and mediated by p53/JNK1 activation promoting a second-round ROS production through a positive feedback loop. These findings extend toxicological knowledge about patulin and cadmium, which may aid in more precise risk assessments of these food contaminants.
Article
Cell Biology
Reina Jochems, Carla Canedo-Ribeiro, Giuseppe Silvestri, Martijn F. L. Derks, Hanne Hamland, Louisa J. Zak, Egbert F. Knol, Alan H. Handyside, Eli Grindflek, Darren K. Griffin
Summary: Preimplantation genetic testing for aneuploidy (PGT-A) is widely used in humans and improves pregnancy and live birth rates in cattle. In pigs, PGT-A offers a potential solution to improve in vitro embryo production (IVP), but the incidence and origin of chromosomal errors in pig embryos have not been well studied. This study used SNP-based PGT-A algorithms to examine natural and IVP porcine embryos and found a higher rate of chromosomal errors in IVP embryos compared to natural embryos. The findings suggest that the high incidence of chromosomal abnormalities may explain the low success of porcine IVP and that PGT-A may have potential for improving embryo transfer success.
Article
Biochemical Research Methods
Xiaohui Zhao, Wanyi Tang, Haipeng Wang, Hao He
Summary: This study shows that a single-time scanning of tightly focused femtosecond laser can modulate the stemness and senescence of tumor cells in vitro and in vivo. The laser-induced cellular senescence and stemness demonstrate distinct transitions in vitro and in vivo. Significant senescence is induced in cells located 1.2 mm deep within the tumor tissue following transient photostimulation in the 100-200 μm shallow layer in vivo, which suppresses the growth of the entire tumor in living mice.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemistry & Molecular Biology
Eisner Salamanca, Yu-Hwa Pan, Ying-Sui Sun, Hao-Wen Hsueh, Odontuya Dorj, Wan-Ling Yao, Jerry Chin-Yi Lin, Nai-Chia Teng, Ikki Watanabe, Shinichi Abe, Yi-Fan Wu, Wei-Jen Chang
Summary: This study aimed to improve the properties of β-TCP by adding magnesium through hydrothermal methods, resulting in enhanced alkaline phosphatase activity and cell viability, promoting bone regeneration.β-TCP/Mg demonstrated potential application prospects in bone grafting and tissue engineering treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Qian Zhou, Gang Fang, Yuzhou Pang, Xueni Wang
Summary: This study found that the combination of docetaxel and kaempferol has a synergistic effect in inhibiting prostate cancer cell proliferation and works by inducing autophagy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Yaiza Nunez, Annabel Garcia-Leon, Aida Falgas, Naroa Serna, Laura Sanchez-Garcia, Ana Garrido, Jorge Sierra, Alberto Gallardo, Ugutz Unzueta, Esther Vazquez, Antonio Villaverde, Ramon Mangues, Isolda Casanova
Summary: Researchers developed a protein nanoparticle, T22-PE24-H6, which can selectively deliver the exotoxin A to CXCR4(+) leukemic cells, and showed potent antitumor activity without toxicity.
Article
Oncology
Julia Schueler, Gabriele Greve, Dorothee Lenhard, Milena Pantic, Anna Edinger, Eva Oswald, Michael Luebbert
Article
Multidisciplinary Sciences
Sandi Grainne Dempsey, Christopher Hamilton Miller, Julia Schueler, Robert W. F. Veale, Darren J. Day, Barnaby C. H. May
Article
Oncology
Indrani Dutta, Dylan Dieters-Castator, James W. Papatzimas, Anais Medina, Julia Schueler, Darren J. Derksen, Gilles Lajoie, Lynne-Marie Postovit, Gabrielle M. Siegers
Summary: Gamma delta T cells (γδ Tc) show promising potential in targeting breast cancer stem-like cells (BCSC), although some BCSC may escape from the immunotherapy. The resistance of BCSC can be partially overcome by regulating MCL-1 and PD-L1, and blocking MICA shedding can increase the sensitivity of BCSC to gamma delta Tc.
Article
Biochemistry & Molecular Biology
Daniel T. Doty, Julia Schueler, Vienna L. Mott, Cassie M. Bryan, Nathan F. Moore, John C. Ho, Jeffrey T. Borenstein
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Jielin Li, Laura Pohl, Julia Schuler, Nina Korzeniewski, Philipp Reimold, Adam Kaczorowski, Weibin Hou, Stefanie Zschabitz, Cathleen Nientiedt, Dirk Jager, Markus Hohenfellner, Anette Duensing, Stefan Duensing
Summary: This study demonstrates the feasibility of patient-individualized in vitro drug screening and preclinical validation, while highlighting the complexities and limitations of this strategy.
Article
Oncology
Matthias Peindl, Claudia Goettlich, Samantha Crouch, Niklas Hoff, Tamara Luettgens, Franziska Schmitt, Jesus Guillermo Nieves Pereira, Celina May, Anna Schliermann, Corinna Kronenthaler, Danjouma Cheufou, Simone Reu-Hofer, Andreas Rosenwald, Elena Weigl, Thorsten Walles, Julia Schueler, Thomas Dandekar, Sarah Nietzer, Gudrun Dandekar
Summary: This study investigates the functions of EMT in tumor invasion and drug resistance using a lung tumor tissue model. The results suggest that EMT is more of a marker for drug resistance rather than a maker, and invasion ability is more dependent on intrinsic factors. EMT is not detected in the center of invasive tumor nodules.
Article
Biochemistry & Molecular Biology
Marnie Newell, Susan Goruk, Julia Schueler, Vera Mazurak, Lynne-Marie Postovit, Catherine J. Field
Summary: This study aimed to explore the interaction between docetaxel (TXT) chemotherapy and docosahexaenoic acid (DHA), and found that DHA can reduce breast cancer tumor growth in preclinical models. The results showed that DHA incorporation is associated with plasma phospholipids and cellular membrane components, and DHA can promote necroptosis. In conclusion, this study confirmed the efficacy of a combination therapy consisting of DHA supplementation and TXT chemotherapy in a TNBC PDX model.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Cell Biology
Bin Xue, Julia Schueler, Christopher M. Harrod, Kanstantsin Lashuk, Zoji Bomya, Kolin C. Hribar
Summary: In this study, a novel 3D in vitro tumor panel comprising 30 distinct PDX models cocultured with fibroblasts and PBMCs in planar extracellular matrix hydrogels was described. The panel represents a significant advancement toward screening in vitro models of the tumor microenvironment and can be used for rapidly screening various combinations and novel agents, accelerating drug discovery for the next generation of therapeutics.
Article
Cell Biology
Meng Dong, Kathrin Boepple, Julia Thiel, Bernd Winkler, Chunguang Liang, Julia Schueler, Emma J. Davies, Simon T. Barry, Tauno Metsalu, Thomas E. Muerdter, Georg Sauer, German Ott, Matthias Schwab, Walter E. Aulitzky
Summary: We developed a perfusion air culture (PAC) system that can maintain the morphology, proliferation, and tumor microenvironment of tumor tissue slices. We found heterogeneous responses to drug treatment between patients by culturing PCTS from mouse xenografts and primary human ovarian tumors and analyzing cleavage of caspase-3 and PD-L1 expression.
Article
Biochemistry & Molecular Biology
Neele Babst, Lisa K. Isbell, Felix Rommel, Aysegul Tura, Mahdy Ranjbar, Salvatore Grisanti, Cordula Tschuch, Julia Schueler, Soroush Doostkam, Peter C. Reinacher, Justus Duyster, Vinodh Kakkassery, Nikolas von Bubnoff
Summary: This study found that CD20+ PCNSL lymphoma cells infiltrating the eye co-expressed distinct homing receptors, such as CXCR4 and CXCR5, in a PVRL homing mouse model. This suggests that these receptors may be involved in the process of PVRL homing into the eye.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)