Article
Cell Biology
Francesca Beretta, Yu-Fang Huang, Anna Rostedt Punga
Summary: Myasthenia gravis is an autoimmune neuromuscular disease without objective biomarkers for treatment decisions. miR-30e-5p may serve as a predictive biomarker for the disease course in MG.
Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Seokju Park, Hee Doo Yang, Jwa-Won Seo, Jin-Wu Nam, Suk Woo Nam
Summary: This article discusses how the editing of microRNAs can lead to the production of isomiRs, which can alter gene regulation networks and contribute to the development of liver cancer. The study also identifies two specific isomiRs associated with liver cancer and highlights their role in suppressing the growth hormone receptor. The results suggest that targeting these isomiRs could be a potential strategy for developing new treatments for liver cancer.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Viviana Costa, Marcello De Fine, Valeria Carina, Alice Conigliaro, Lavinia Raimondi, Angela De Luca, Daniele Bellavia, Francesca Salamanna, Riccardo Alessandro, Giovanni Pignatti, Milena Fini, Gianluca Giavaresi
Summary: The research focuses on the role of miR-31-5p and miR-33a-5p in osteoarthritis progression, with identification of Specificity proteins (Sp1) as common miRNA targets through bioinformatic analysis. The study evaluates the possible mechanism of how these miRNAs regulate Sp1 to affect CX43 expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Gastroenterology & Hepatology
Pedro M. Rodrigues, Marta B. Afonso, Andre L. Simao, Tawhidul Islam, Maria M. Gaspar, Colm J. O'Rourke, Monika Lewinska, Jesper B. Andersen, Enara Arretxe, Cristina Alonso, Alvaro Santos-Laso, Laura Izquierdo-Sanchez, Raul Jimenez-Aguero, Emma Eizaguirre, Luis Bujanda, Maria J. Pareja, Carina Prip-Buus, Jesus M. Banales, Cecilia M. P. Rodrigues, Rui E. Castro
Summary: This study evaluated the role of hsa-miRNA-21-5p in the development of hepatocellular carcinoma (HCC) in non-alcoholic fatty liver disease (NAFLD) patients. The expression of hsa-miRNA-21-5p was found to increase with NAFLD severity and was associated with hepatic lipotoxicity. Inhibition of hsa-miRNA-21-5p was shown to prevent the progression of NAFLD to NASH and HCC.
LIVER INTERNATIONAL
(2023)
Editorial Material
Cell Biology
Meredith Hill, Nham Tran
Summary: miRNAs play a crucial role in altering cellular environment by regulating target genes, and they may also influence other miRNAs and mRNA expression. This study focuses on exploring the regulatory effects of oncogenic miR-21 and its impact on other miRNA species.
TRENDS IN CELL BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Miruna Nemecz, Diana Simona Stefan, Ioana Karla Comarita, Alina Constantin, Gabriela Tanko, Cristian Guja, Adriana Georgescu
Summary: In this study, miR-218, miR-132, and miR-143 were identified as significantly up-regulated miRNAs in both circulating MVs and plasma from diabetic patients with dyslipidemia. These miRNAs showed significant correlations with important plasma markers representative of this pathology.
CARDIOVASCULAR DIABETOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Xiaoqin Li, Xinyue Tang, Kunsong Li, Ling Lu
Summary: This study suggests that serum miR-9-5p and miR-148a-3p levels may serve as noninvasive biomarkers for the early diagnosis of breast cancer.
BIOMED RESEARCH INTERNATIONAL
(2022)
Article
Medicine, Research & Experimental
Hongmei Zhao, Hongqin Yang, Chi Geng, Yang Chen, Yaqin Tang, Zhiwei Li, Junling Pang, Ting Shu, Yu Nie, Yongshuo Liu, Kegang Jia, Jing Wang
Summary: Research shows that elevated IgE promotes pathological cardiac fibrosis by modulating miR-486a-5p and downstream factors, such as Smad1, providing new targets for intervention in pathological cardiac fibrosis.
Article
Medicine, Research & Experimental
Zheng He, Meng Tian, Xuan Fu
Summary: miR-30c-5p downregulation in hepatocellular carcinoma is significantly associated with patient survival and tumor size, affecting cell proliferation and migration, with RAB32 identified as a crucial target. These findings highlight the important role of miR-30c-5p in HCC growth and invasion through the miR-30c-5p-RAB32 axis.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Genetics & Heredity
Tamyres Mingorance Carvalho, Guillermo Ortiz Brasil, Tayana Schultz Jucoski, Douglas Adamoski, Rubens Silveira de Lima, Cleverton C. Spautz, Karina Furlan Anselmi, Patricia Midori Murobushi Ozawa, Iglenir Joao Cavalli, Jaqueline Carvalho de Oliveira, Daniela Fiori Gradia, Enilze Maria de Souza Fonseca Ribeiro
Summary: The study identified miR-320a and miR-4433b-5p as highly specific and sensitive biomarkers for distinguishing breast cancer from controls, regardless of sample source. In tissues, lower levels of miR-150-5p and higher levels of miR-142-5p were significant biomarkers. Combining these miRNAs in panels improved the ability to differentiate breast cancer patients from controls.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Huijie Guo, Mei Pu, Yusi Tai, Yuxiang Chen, Henglei Lu, Junwen Qiao, Guanghui Wang, Jing Chen, Xinming Qi, Ruimin Huang, Zhouteng Tao, Jin Ren
Summary: The study revealed that miR-30b-5p can inhibit TFEB-dependent gene transcription by binding to CLEAR elements in the nucleus, thus affecting lysosomal biogenesis and autophagy. Knocking out miR-30b-5p increases TFEB transactivation, leading to increased expression of autophagy and lysosomal biogenesis-related genes.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Biochemistry & Molecular Biology
Ping Xue, Saihua Huang, Xiao Han, Caiyan Zhang, Lan Yang, Wenfeng Xiao, Jinrong Fu, Hao Li, Yufeng Zhou
Summary: Exosomal miRNAs, such as miR-101-3p and miR-423-5p, play important roles as tumor suppressors in mediating the progression of medulloblastoma by inhibiting proliferation, migration, and invasion of tumor cells, and promoting apoptosis. These exosomal miRNAs can be delivered to tumor cells via exosomes and directly target genes like FOXP4 and EZH2, reinforcing their tumor inhibitory effects. Overexpression of miR-101-3p and miR-423-5p has been shown to inhibit tumorigenesis in vivo in a xenograft mouse model, indicating a potential therapeutic approach for the treatment of children with medulloblastoma.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Biochemistry & Molecular Biology
Juana Moro, Agustina Grinpelc, Paula Lucia Farre, Rocio Belen Duca, Ezequiel Lacunza, Karen Daniela Grana, Georgina Daniela Scalise, Guillermo Nicolas Dalton, Cintia Massillo, Flavia Piccioni, Federico Dimase, Emilio Batagelj, Adriana De Siervi, Paola De Luca
Summary: This study identified circulating miRNAs associated with metabolic syndrome (MS) that also impact on breast cancer (BC), particularly triple-negative breast cancer (TNBC). The increased expression of miR-877-5p was found to be associated with poor patient survival and higher prevalence in basal-like BC tumors. The inhibition of miR-877-5p could be a potential therapeutic option for TNBC treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Fabiola Olivieri, Francesco Prattichizzo, Angelica Giuliani, Giulia Matacchione, Maria Rita Rippo, Jacopo Sabbatinelli, Massimiliano Bonafe
Summary: Inflammaging is a key concept in understanding how aging contributes to the development of age-related diseases, with a focus on the role of miRNAs in regulating inflammatory pathways. The assessment of individual genetic make-up alone is insufficient to depict the trajectories of age-related inflammation, and studies have shown that circulating inflammamiRs could be used to measure the degree of inflammaging.
AGEING RESEARCH REVIEWS
(2021)
Article
Medicine, Research & Experimental
Ahmed I. Abulsoud, Hussein M. El-Husseiny, Ahmed A. El-Husseiny, Hesham A. El-Mahdy, Ahmed Ismail, Samy Y. Elkhawaga, Emad Gamil Khidr, Doaa Fathi, Eman A. Mady, Agnieszka Najda, Mohammad Algahtani, Abdulrahman Theyab, Khalaf F. Alsharif, Ashraf Albrakati, Roula Bayram, Mohamed M. Abdel-Daim, Ahmed S. Doghish
Summary: COVID-19 is a global pandemic caused by SARS-coronavirus-2 (SARS-CoV-2). The development of new vaccines with innovative technologies has revolutionized the field of vaccinology. These vaccines use mRNA encoding the Spike SARS-CoV-2 protein or recombinant adenovirus vectors to introduce the Spike protein gene into cells, allowing for faster updates to combat new variants. However, effective control of these variants requires a rapid and widespread vaccination policy with sufficient vaccine doses.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Medicine, Research & Experimental
Ahmed S. Doghish, Ahmed Ismail, Hesham A. El-Mahdy, Samy Y. Elkhawaga, Elsayed G. E. Elsakka, Eman A. Mady, Mahmoud A. Elrebehy, Mahmoud A. F. Khalil, Hussein M. El-Husseiny
Summary: Rheumatoid arthritis (RA) is a severe autoimmune inflammation that primarily affects the joints. miRNAs, small noncoding molecules, are involved in its pathogenesis by disrupting immune balance and triggering inflammation. Additionally, miRNAs have potential applications in RA diagnosis, prognosis, and treatment.
Article
Medicine, Research & Experimental
Samy Y. Elkhawaga, Ahmed Ismail, Elsayed G. E. Elsakka, Ahmed S. Doghish, Mohamed A. Elkady, Hesham A. El-Mahdy
Summary: In recent decades, obesity has become a pandemic issue with significant impact on quality of life and life expectancy due to its association with serious co-morbidities. Obesity is influenced by various factors including age, sex, endocrine and genetic factors. MiRNAs, as genetic factors, have recently emerged as important regulators of obesity. MiRNAs are small noncoding nucleic acids that can modulate gene expression and affect biological processes relevant to obesity, such as adipogenesis, lipid metabolism and homeostasis. This review provides an updated overview of the roles of miRNAs in obesity and its processes and highlights their potential applications as therapeutic targets for obesity.
Review
Endocrinology & Metabolism
Ahmed Ismail, Hesham A. El-Mahdy, Mahmoud Gomaa Eldeib, Ahmed S. Doghish
Summary: Diabetes mellitus is often accompanied by microvascular complications such as nephropathy, retinopathy, and neuropathy. MicroRNAs (miRNAs) play a crucial role in the pathogenesis of these complications by affecting the kidney, retina, and peripheral neurons through various molecular pathways. This review provides an updated understanding of the involvement of miRNAs in diabetic microvascular complications, with a focus on the key molecular pathways mediating the adverse effects of hyperglycemia on vascular tissues. The review highlights specific miRNAs (miR-126, miR-29b, miR-125a, and miR-146a) and their impact on vascular endothelial growth factors.
MOLECULAR GENETICS AND METABOLISM
(2023)
Article
Medicine, Research & Experimental
Shady Allam, Elsayed G. E. Elsakka, Ahmed Ismail, Ahmed S. Doghish, Amr Mohamed Yehia, Mohamed A. Elkady, Hamada Ahmed Mokhlis, Sara M. Sayed, Adel I. Abd Elaziz, Abdullah A. Hashish, Mona M. Amin, Rehab Mohamed El Shahat, Osama A. Mohammed
Summary: This study aimed to investigate the effect of flutamide, an androgen receptor (AR) blocker, on renal pathologic changes such as inflammation, apoptosis, and fibrosis in male rats. The study found that AR blockade resulted in the down-regulation of markers associated with renal inflammation, apoptosis, and fibrosis. Additionally, it was found that the blockade increased the expression of chloride channel protein-5 (ClC-5) and pAKT proteins. This research suggests that AR blockade may be an effective strategy for mitigating renal side effects caused by certain drugs.
Review
Medicine, Research & Experimental
Doaa Fathi, Mohammed S. Elballal, Ahmed E. Elesawy, Ahmed I. Abulsoud, Ahmed Elshafei, Elsayed G. E. Elsakka, Ahmed Ismail, Hesham A. El-Mahdy, Mahmoud A. Elrebehy, Ahmed S. Doghish
Summary: Gastric cancer (GC) ranks fourth globally in terms of incidence and mortality rates. MicroRNAs (miRNAs), among other genetic and epigenetic factors, have been found to impact the initiation and progression of GC. Dysregulation of miRNA expressions is associated with various aspects of GC, including initiation, invasion, apoptosis evasion, angiogenesis, promotion, and epithelial-mesenchymal transition enhancement. Important pathways controlled by miRNAs in GC include Wnt/8-catenin signaling, HMGA2/mTOR/P-gp pathway, PI3K/AKT/c-Myc pathway, VEGFR, and TGFb signaling. Hence, this review aims to provide an updated understanding of the role of miRNAs in GC pathogenesis and their modulatory effects on different GC treatment modalities.
Article
Pathology
Ola Elazazy, Heba M. Midan, Reem K. Shahin, Ahmed E. Elesawy, Mohammed S. Elballal, Al-Aliaa M. Sallam, Abdullah M. M. Elbadry, Mahmoud A. Elrebehy, Abdelmenem Bhnsawy, Ahmed S. Doghish
Summary: Long non-coding RNAs (lncRNAs), with a length larger than 200 nucleotides, play a role in controlling gene expression and have been found to be abnormally expressed in rheumatoid arthritis (RA) patients. These lncRNAs have potential as biomarkers and treatment targets for diagnosing and treating RA. This review focuses on the pathogenesis of RA, clinical implications, and related lncRNA expressions for the identification of new biomarkers and treatment targets.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Review
Pathology
Ahmed S. Doghish, Hesham A. El-Mahdy, Ahmed Ismail, Maghawry Hegazy, Mahmoud Mohamed Mokhtar, Samy Y. Elkhawaga, Mohamed A. Elkady, Amr Mohamed Yehia, Elsayed G. E. Elsakka
Summary: Thyroid cancer is the most common endocrine malignant tumor, with various types such as papillary thyroid cancer and follicular thyroid carcinoma. Risk factors for thyroid cancer include radiation exposure, autoimmunity, and genetics. MicroRNA plays a crucial role in thyroid cancer by inhibiting the expression of multiple target genes and influencing tumor proliferation, angiogenesis, apoptosis, development, and resistance to therapy.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Pathology
Ahmed S. Doghish, Maghawry Hegazy, Ahmed Ismail, Hesham A. El-Mahdy, Elsayed G. E. Elsakka, Samy Y. Elkhawaga, Mohamed A. Elkady, Amr Mohamed Yehia, Nourhan M. Abdelmaksoud, Mahmoud Mohamed Mokhtar
Summary: Osteosarcoma (OS) is a common bone cancer in children, teenagers, and young adults, and various epigenetic elements, including miRNAs, have been found to affect OS progression, initiation, angiogenesis, and treatment resistance. MiRNAs can regulate the expression of genes involved in OS pathogenesis and play roles in invasion, angiogenesis, metastasis, proliferation, cell cycle, and apoptosis. MiRNAs also impact important OS-related signaling pathways, such as WNT/β-catenin, PTEN/AKT/mTOR, and KRAS mutations. Furthermore, miRNAs may influence the response of OS to therapies like radiotherapy and chemotherapy. This review focuses on the relationship between miRNAs and OS by exploring their effects on OS signaling pathways.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Review
Pathology
Tohada M. Al-Noshokaty, Abdallah Mansour, Rehab Abdelhamid, Nourhan Abdellatif, Ayat Alaaeldien, Tasnim Reda, Nourhan M. Abdelmaksoud, Ahmed S. Doghish, Ahmed I. Abulsoud, Shereen Saeid Elshaer
Summary: Pancreatic cancer is a deadly cancer with poor prognosis, mainly due to the lack of reliable early detection methods. Long non-coding RNAs (LncRNAs) are protein-free RNAs produced by genome transcription and play critical roles in gene expression regulation, cell proliferation, and differentiation. Recent research has revealed that LncRNAs also play important regulatory roles in the development and progression of pancreatic cancer. This review discusses the various roles of LncRNAs in the molecular mechanisms underlying pancreatic cancer and their involvement in pathogenesis and treatment resistance.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Pathology
Shereen Saeid Elshaer, Ahmed I. Abulsoud, Doaa Fathi, Nourhan M. Abdelmaksoud, Mohamed Bakr Zaki, Hesham A. El-Mahdy, Ahmed Ismail, Elsayed G. E. Elsakka, Mai A. Abd-Elmawla, Logyna A. Abulsoud, Ahmed S. Doghish
Summary: This review summarizes the role of microRNAs (miRNAs) in the development of glioblastoma (GBM) and discusses their potential as prognostic biomarkers and therapeutic targets.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Biochemistry & Molecular Biology
Ahmed Doghish, Amr Shehabeldine, Hesham El-Mahdy, Mahmoud Hassanin, Abdulaziz Al-Askar, Samy Marey, Hamada Abdelgawad, Amr Hashem
Summary: In this study, a thyme essential oil nanoemulsion was successfully prepared using a safe and eco-friendly method. The nanoemulsion exhibited antibacterial, antifungal, and anticancer activities, making it a promising candidate for further research and development.
Article
Biochemistry & Molecular Biology
Amr M. Shehabeldine, Ahmed S. Doghish, Walaa A. El-Dakroury, Mahmoud M. H. Hassanin, Abdulaziz A. Al-Askar, Hamada AbdElgawad, Amr H. Hashem
Summary: In this study, clove oil nanoemulsion (CL-nanoemulsion) and emulsion (CL-emulsion) were prepared using an ecofriendly method. The CL-nanoemulsion showed antibacterial, antifungal, anti-biofilm, and anticancer properties, with minimal inhibitory concentration (MIC) values between 0.31 and 5 mg/mL against bacteria and significant antifungal activity against various fungal strains. The CL-nanoemulsion also exhibited lower IC50 values in MCF-7 breast cancer cells compared to CL-emulsion, indicating its potential as an anticancer agent.
Article
Pathology
Ahmed A. El-Husseiny, Nourhan M. Abdelmaksoud, Sherif S. Abdel Mageed, Aya Salman, Mohamed Bakr Zaki, Hesham A. El-Mahdy, Ahmed Ismail, Mai A. Abd-Elmawla, Hussein M. El-Husseiny, Ahmed I. Abulsoud, Shereen Saeid Elshaer, Elsayed G. E. Elsakka, Doaa Fathi, Walaa A. El-Dakroury, Ahmed S. Doghish
Summary: Salivary gland cancer is a type of head and neck cancer with poor survival rates for nonresectable tumors. Research on the pathophysiology of this cancer, particularly at the molecular level, is needed. Abnormal levels of microRNAs (miRNAs) have been found in salivary cancer tissues, suggesting their crucial role in the carcinogenesis of this cancer. Several studies have also identified potential miRNA biomarkers and therapeutic targets for salivary gland cancer. This review aims to explore the regulatory impact of miRNAs on the molecular pathology of this cancer and provide an up-to-date summary of relevant literature.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Review
Pathology
Amr Mohamed Yehia, Elsayed G. E. Elsakka, Ahmed I. Abulsoud, Nourhan M. Abdelmaksoud, Ahmed Elshafei, Samy Y. Elkhawaga, Ahmed Ismail, Mahmoud Mohamed Mokhtar, Hesham A. El-Mahdy, Maghawry Hegazy, Mohammed S. Elballal, Osama A. Mohammed, Hussein M. El-Husseiny, Heba M. Midan, Walaa A. El-Dakroury, Moataz B. Zewail, Sherif S. Abdel Mageed, Yasser M. Moustafa, Randa M. Mostafa, Mohamed A. Elkady, Ahmed S. Doghish
Summary: Multiple myeloma is a extensively studied cancer of plasma cells, and researchers are increasingly interested in the role of microRNAs in regulating gene expression in this disease. Non-coding RNAs have been shown to play a crucial role in regulating MM pathogenesis signaling pathways, including Wnt/β-catenin, PI3K/Akt/mTOR, P53, and KRAS. This review emphasizes the importance of miRNAs in MM signaling and their potential as therapeutic targets.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
