Journal
BRAIN RESEARCH
Volume 1748, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.brainres.2020.147070
Keywords
Hypoxic-ischemic encephalopathy; Non-coding RNA; Vof-16 knockout; Behavioral evaluations
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Funding
- Program of Science & Technology Department of Sichuan Province [2020YFS0043]
- National construction project of regional Chinese medicine treatment centre [2018205]
- National construction project of the second clinical research base of Chinese medicine [2018131]
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Hypoxic-ischemic encephalopathy (HIE) results in high neonatal mortality and severe neurological impairments, and its underlying molecular mechanism underwent extensive investigations. Long non-coding RNA (lncRNA) is considered to be an important regulator on brain development and many neurological diseases. Currently, little is known about the role of Vof-16 (lncRNA) in HIE. We detected the relative expression level of Vof-16 in the cortex and hippocampus of hypoxic-ischemic (HI) models whose successful establishment was verified by TTC staining. Then, Vof-16 knockout rats were generated using the CRISPR/Cas engineering technology to search the specific function of the Vof-16 through a series of behavioral evaluations including Neurological severity scores (NSS), Y-maze test, Morris water maze (MWW) test, open field test, and Rotarod test. The results demonstrated the expression of Vof-16 was substantially up-regulated in the cortex and hippocampus of rats with HI injury. Importantly, Vof-16 knockout facilitated the recovery from long-term HI induced nerve damage and neurobehavioral dysfunctions. In conclusion, this study suggests Vof-16 knockout is a promising treatment target for neonatal
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