Article
Reproductive Biology
F. Hassan, W. Holtz
Summary: Through a study involving radioactively labeled thymidine infusion in 48 sexually rested young adult boars, it was discovered that labeled sperm first appeared in the proximal caput after 28 days, then reached the distal caput after 2 days, with the majority found in the corpus after another 2 days. Progress was halted at the transition of corpus and cauda on Day 11, but resumed on Day 12 and by Day 13 sperm had exited the epididymis via the ductus deferens.
Article
Genetics & Heredity
Mazdak Salavati, Shernae A. Woolley, Yennifer Cortes Araya, Michelle M. Halstead, Claire Stenhouse, Martin Johnsson, Cheryl J. Ashworth, Alan L. Archibald, Francesc X. Donadeu, Musa A. Hassan, Emily L. Clark
Summary: This research identified regulatory sequences in the pig genome through Omni-ATAC-Seq analysis of semitendinosus muscle from 24 male piglets. Over 50% of the open chromatin regions were found near known transcription start sites. Differential analysis revealed genomic regions with significantly different chromatin accessibility across developmental time points.
G3-GENES GENOMES GENETICS
(2022)
Article
Biology
Yan Gong, Jun He, Biao Li, Yu Xiao, Qinghua Zeng, Kang Xu, Yehui Duan, Jianhua He, Haiming Ma
Summary: This study reveals the transcription profiles and functional networks in lncRNA and mRNA in subcutaneous adipose tissue of Ningxiang piglets at different developmental stages. Most DEmRNAs were up-regulated in piglets, while they were down-regulated in late fattening stages, with DElncRNAs being up-regulated in late fattening stages. Modules 2 and 5 exhibit similar transcription modes, mainly involved in steroid biosynthesis, glycosphingolipid biosynthesis, metabolic pathways, and glycerolipid metabolism.
Article
Biochemistry & Molecular Biology
Yueyun Ding, Yinhui Hou, Zijing Ling, Qiong Chen, Tao Xu, Lifei Liu, Na Yu, Wenliang Ni, Xiaoling Ding, Xiaodong Zhang, Xianrui Zheng, Wenbin Bao, Zongjun Yin
Summary: This study assessed the whole transcriptome expression profiles of pigs' Longissimus dorsi muscle with high and low IMF content to identify genes involved in porcine IMF adipogenesis. The findings revealed the involvement of non-coding RNAs in fatty acid metabolic processes, lipid metabolism, and fat cell differentiation. The study also constructed competing endogenous RNAs (ceRNA) regulatory networks, highlighting the roles of lncRNAs, circRNAs, and miRNAs in IMF deposition. These findings provide potential regulators and molecular regulatory networks for future research on IMF traits in pigs, which can aid in improving pork quality through marker-assisted selection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Yuxiu Sun, Chen Li, Qingyi Lu, Haixu Jiang, Mengmeng Zhu, Guangrui Huang, Ting Wang
Summary: SAPHO syndrome is characterized by inflammatory lesions on bones and skin, with diverse clinical manifestations and lack of molecular biomarkers. RNA sequencing and bioinformatics analysis identified differentially expressed mRNAs and lncRNAs, and suggested potential biomarkers for diagnosis. This study provides insight into the transcriptome profile changes associated with SAPHO syndrome and offers inspiration for further research on clinical biomarkers and molecular regulators.
FRONTIERS IN GENETICS
(2021)
Article
Nutrition & Dietetics
Zhong Xu, Junjing Wu, Jiawei Zhou, Yu Zhang, Mu Qiao, Hua Sun, Zipeng Li, Lianghua Li, Nanqi Chen, Favour Oluwapelumi Oyelami, Xianwen Peng, Shuqi Mei
Summary: This study used ATAC-seq and RNA-seq analysis to examine the accessible chromatin and gene expression in Xidu black pigs, identifying key genes and regulatory pathways associated with meat quality and fat deposition.
FRONTIERS IN NUTRITION
(2022)
Article
Cell Biology
Foyez Shams, Darryl D'Souza, Tariq Ezaz
Summary: In a study conducted on a representative sample of 94 boars from a commercial nucleus herd in Australia, it was found that 6.38% of the samples had novel chromosome rearrangements associated with hypoprolificacy. These rearrangements, including a previously detected translocation between chromosomes 3 and 16, were identified through standard and molecular cytogenetic analyses. Further research and routine monitoring will be essential to prevent the propagation of reciprocal translocations in the Australian pig population.
Article
Agriculture, Dairy & Animal Science
Rie Saito, Natsuko Ito Kondo, Yui Nemoto, Reiko Kumada, Nobuyoshi Nakajima, Masanori Tamaoki
Summary: After the Fukushima nuclear incident, the number of wild boars in the DRZ area increased significantly, raising concerns about the spread of highly contaminated boars to other areas. This study used MIG-seq analysis to uncover the dispersal and gene flow of wild boar population in Fukushima and found significant genetic differences between the eastern and western groups, divided by the Abukuma River. The study suggests that the river and urbanization along it may affect the migration of wild boars.
Article
Biochemistry & Molecular Biology
Huashui Ai, Mingpeng Zhang, Bin Yang, Amy Goldberg, Wanbo Li, Junwu Ma, Debora Brandt, Zhiyan Zhang, Rasmus Nielsen, Lusheng Huang
Summary: Research on Eurasian pig populations revealed significant genetic haplotype differences and hybridization history. By analyzing Y chromosome data, the study uncovered three distinct waves of European gene introgression into Asian pig populations in the last 200 years, as well as the presence and distribution of European Y chromosomes in Chinese pig breeds. The European Y haplotype in Chinese pigs is associated with lean meat production, suggesting artificial selection as a driving force for its increased frequency.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Agriculture, Dairy & Animal Science
Bo Zhang, Zunqiang Yan, Pengfei Wang, Qiaoli Yang, Xiaoyu Huang, Haixia Shi, Yuran Tang, Yanan Ji, Juanli Zhang, Shuangbao Gun
Summary: Chinese Hezuo (HZ) boars are known for their early sexual maturity, with histological analysis confirming precocious puberty characteristics. Key long noncoding RNA (lncRNA) and mRNA identified in testes of immature and mature HZ boars could play a crucial role in precocious puberty regulation and accelerate breeding processes in livestock production.
Article
Genetics & Heredity
Yibing Liu, Ying Yu, Hong Ao, Fengxia Zhang, Xitong Zhao, Huatao Liu, Yong Shi, Kai Xing, Chuduan Wang
Summary: This study identified various lncRNAs in pigs that play a role in fat deposition and metabolism through RNA-seq. Further analysis revealed that some lncRNAs can affect swine backfat deposition by regulating target genes.
Article
Genetics & Heredity
Xiuxiu Zhang, Wanlong Huang, Yuntao Guo, Xiangyang Miao
Summary: This study aimed to explore the regulatory functions of miRNAs in fat deposition for the prevention and therapy of lipid metabolism-related diseases. Significant differences in fat deposition between Laiwu and Large White pigs were found, with 39 known miRNAs and 56 novel miRNAs differentially expressed between the two breeds. The predicted targets of these differentially expressed miRNAs were involved in several fat-associated pathways, such as the PPAR, MAPK, and Wnt signaling pathways.
Article
Behavioral Sciences
Henk-Jan Boele, Sangyun Joung, Joanne E. Fil, Austin T. Mudd, Stephen A. Fleming, Sebastiaan K. E. Koekkoek, Ryan N. Dilger
Summary: By conducting eyeblink conditioning experiments on 3-week-old pigs, it has been demonstrated for the first time that young pigs have the potential to be a valuable behavioral tool in measuring neurodevelopment through cerebellar classical conditioning tasks.
FRONTIERS IN BEHAVIORAL NEUROSCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Chenxi Zhang, Ying Zhou, Bin Zhang, Zhihong Sheng, Nan Sun, Baiyin Yuan, Xiaoyuan Wu
Summary: In this study, the expression profiles of lncRNAs, miRNAs, and mRNAs in SCLC tumors and adjacent non-cancerous tissues were comprehensively analyzed. A ceRNA network consisting of 9 lncRNAs, 11 miRNAs, and 392 mRNAs was constructed through bioinformatics analysis. The study also identified the potential role of lncRNA TCONS_00020615 in regulating SCLC tumorigenesis through the TCONS_00020615-hsa-miR-26b-5p-TPD52 pathway.
Article
Endocrinology & Metabolism
Hui Feng, Tianyi Liu, Salsabeel Yousuf, Xiuxiu Zhang, Wanlong Huang, Ai Li, Lingli Xie, Xiangyang Miao
Summary: Through deep RNA sequencing, we identified differentially expressed lncRNAs, miRNAs, and mRNAs in subcutaneous and intramuscular adipose tissues in pigs with high meat quality. Bioinformatics analysis revealed key lncRNAs, miRNAs, and mRNAs involved in lipid metabolism and adipogenesis. We found that the Wnt signaling pathway plays a critical regulatory role in intramuscular adipogenesis and lipid accumulation.
FRONTIERS IN ENDOCRINOLOGY
(2023)