Microbial bioconversion of feathers into antioxidant peptides and pigments and their liposome encapsulation

Objectives The co-encapsulation of bioactive peptides obtained from degradation of chicken feathers and flexirubin-type pigment produced by Chryseobacterium sp. kr6 into phosphatidylcholine liposomes was investigated. Results Control empty liposomes showed mean diameter of 168.5 nm, varying to 185.4, 102.0 and 98.5 nm after the encapsulation of peptides, pigment and their co-encapsulation, respectively. Control liposomes presented zeta potential of - 20.9 mV, while the formulations containing the bioactive compounds showed values of - 30 mV or higher in magnitude. Infrared analysis revealed typical spectra for phosphatidylcholine, suggesting that no new chemical bonds were formed after encapsulation. ABTS radical scavenging assay showed that the antioxidant activity of the compounds was maintained after encapsulation. Conclusions Feather waste can be a valuable substrate for simultaneous production of antioxidant peptides and pigment by Chryseobacterium sp. kr6, and their encapsulation into liposomes may be a suitable alternative for delivery of these natural antioxidants.

Automated summary

This study investigated the co-encapsulation of bioactive peptides from chicken feathers and flexirubin-type pigment from Chryseobacterium sp. kr6 into phosphatidylcholine liposomes. The results showed that the antioxidant activity of the compounds was maintained after encapsulation. Therefore, feather waste can be a valuable substrate for simultaneous production of antioxidant peptides and pigment, and their encapsulation into liposomes may be a suitable delivery method for these natural antioxidants.