Article
Microbiology
Christine E. Broster Reix, Miharisoa Rijatiana Ramanantsalama, Carmelo Di Primo, Laetitia Minder, Melanie Bonhivers, Denis Dacheux, Derrick R. Robinson
Summary: Trypanosoma brucei, a genus of protists in Sub-Saharan Africa, has surface glycoproteins that aid in immune system evasion. Importantly, the flagellar pocket contains key cytoskeletal components like TbBILBO1. Nanobodies raised against TbBILBO1 proved lethal to parasites, demonstrating the potential for antibody-derived intrabodies in targeting trypanosome cytoskeleton proteins.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemistry & Molecular Biology
Nori Nakai, Keisuke Sato, Tomomi Tani, Masahiko Kawagishi, Hiromasa Ka, Kenta Saito, Sumio Terada
Summary: POLArIS is a versatile probe for monitoring the dipole orientation of target molecules using fluorescence polarization microscopy. New versions of POLArIS with nanobodies and extended color variations enable multi-color orientation imaging for multiple targets, enhancing the probe's performance for broader applications in living cells, tissues, and whole organisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biology
John G. Dingus, Jonathan C. Y. Tang, Ryoji Amamoto, Grace K. Wallick, Constance L. Cepko, Liqun Luo
Summary: Conventional antibodies and their derived fragments face challenges in targeting intracellular sites in live cells due to their larger size and complex structure. In contrast, nanobodies offer an alternative approach with documented success in intracellular expression. However, a systematic study on the intracellular expression of nanobodies has been lacking. This study investigated the intracellular expression of 75 nanobodies from the Protein Data Bank and found that a majority of them were unstable in cells, leading to aggregation and clearance. By using comparative analysis and framework mutagenesis, a general approach was developed to stabilize these originally unstable nanobodies without compromising their target binding. The stability of the mutated nanobodies extended to in vivo contexts, demonstrating the potential for improved nanobody engineering in intracellular applications. This study contributes to the growing field of intracellular interrogation and intervention.
Review
Neurosciences
Henry M. Colecraft, James S. Trimmer
Summary: Selective ion channel modulators are important in studying physiological function and developing therapeutic strategies. Renewable and recombinant antibodies can be used to control ion channel function, and this review discusses their different forms and mechanisms. Intrabodies, a type of recombinant antibody expressed intracellularly, are highlighted as a genetic tool to control ion channel function. The review also suggests potential future research directions for engineering recombinant antibodies for enhanced utility in ion channel research.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Biochemistry & Molecular Biology
Teresa R. Wagner, Ulrich Rothbauer
Summary: With the development of nanobodies, the study and modulation of target antigens in living cells has become more feasible, with Nbs also being used as research tools and biosensors. Target-specific Nbs can achieve protein knockouts, while modulating Nbs have the potential to become future therapeutic drugs.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Chemistry, Physical
Karine Queiroz Zetune Villa Real, Nikolaos Mougios, Ronja Rehm, Shama Sograte-Idrissi, Laszlo Albert, Amir Mohammad Rahimi, Manuel Maidorn, Jannik Hentze, Markel Martinez-Carranza, Hassan Hosseini, Kim-Ann Saal, Nazar Oleksiievets, Matthias Prigge, Roman Tsukanov, Pal Stenmark, Eugenio F. Fornasiero, Felipe Opazo
Summary: Living synapse imaging has traditionally relied on fluorescently tagged synaptic proteins, but this approach can alter synapse physiology. To overcome these limitations, a noninvasive nanobody (iNbSyt1) that binds to synaptotagmin-1 has been developed. This nanobody allows for the generation of protein-based fluorescent reporters and is ideal for super-resolution imaging methods. Overall, nanobody NbSyt1 enables precise imaging in cellular and molecular neuroscience across multiple spatiotemporal scales.
Article
Biochemistry & Molecular Biology
Yuta Ishizuka, Tadiwos F. Mergiya, Rodolfo Baldinotti, Ju Xu, Erik Hallin, Sigurbjorn Markusson, Petri Kursula, Clive R. Bramham
Summary: Arc protein plays important roles in synaptic plasticity, memory, and cognitive flexibility. This study developed anti-Arc nanobodies as new tools to investigate Arc dynamics and function, and found that ArcNbs have versatile applications.
NEUROCHEMICAL RESEARCH
(2022)
Review
Cell Biology
James S. Trimmer
Summary: Intrabodies are recombinant antibodies designed for expression within neurons, allowing for precise targeting of specific molecules and subcellular domains to study and manipulate neuronal function with high precision.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Genetics & Heredity
Juan Yin, Fuyi Tong, Yulan Ye, Tong Hu, Lijuan Xu, Liping Zhang, Jianyun Zhu, Zhi Pang
Summary: Circular RNA hsa_circRNA_103124 was found to promote cell proliferation and inhibit autophagy in patients with Crohn's disease by regulating the hsa-miR-650/AKT2 signaling pathway.
FRONTIERS IN GENETICS
(2021)
Article
Plant Sciences
Matheus Scherer Bastos, Rafaela Mallmann Saalfeld, Bruna Pasqualotto Costa, Maria Claudia Garcia, Krist Helen Antunes, Ketlin Fernanda Rodrigues, Denizar Melo, Eliane Romanato Santarem, Jarbas Rodrigues de Oliveira
Summary: This study aimed to use the ethanolic extract and fractions of Moquiniastrum polymorphum subsp. polymorphum in the treatment of hepatic fibrosis, and the results suggest a potential therapeutic effect against liver fibrosis.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Joaquim Grego-Bessa, Paula Gomez-Apinaniz, Belen Prados, Manuel Jose Gomez, Donal MacGrogan, Jose Luis de la Pompa
Summary: This study investigated the functions and downstream mechanisms of Nrg1 signaling during ventricular chamber development. The Nrg1-ErbB2/ErbB4-Erk axis was found to be a crucial regulator of cardiomyocyte cell cycle progression and migration.
CIRCULATION RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Evan Ja-Yang Wang, I-Hsuan Chen, Brian Yu-Ting Kuo, Chia-Cheng Yu, Ming-Tsung Lai, Jen-Tai Lin, Leo Yen-Ting Lin, Chih-Mei Chen, Tritium Hwang, Jim Jinn-Chyuan Sheu
Summary: Cytoskeleton proteins provide mechanical support for cell development and tissue homeostasis. Mutated genes associated with cytoskeletal proteins have been found in cancer, some of which have clinical implications. Cytoskeleton-associated proteins also play important signaling roles in cancer development. Mutations in cytoskeleton components can change the physical/mechanical properties of cells and determine cell fate during cancer development.
Article
Biochemistry & Molecular Biology
Tai-Hsin Tsai, Yu-Feng Su, Cheng-Yu Tsai, Chieh-Hsin Wu, Kuan-Ting Lee, Yi-Chiang Hsu
Summary: RTA dh404 is a novel synthetic oleanolic acid derivative with various properties and therapeutic effects on cancers. It reduces glioma cell viability and induces apoptosis, autophagy, and cell cycle arrest in glioblastoma cells. These effects are mediated by the regulation of associated genes, indicating the potential of RTA dh404 as a drug candidate for glioblastoma treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Xiangsheng Zhang, Huan Kan, Yun Liu, Wu Ding
Summary: Plumbagin shows potential medicinal properties, including anticancer activities. It inhibits cell proliferation, blocks cell cycle, induces apoptosis and autophagy in endometrial cancer cells. The anticancer effects of PLB may be mediated by abrogating the PI3K/Akt pathway.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Environmental Sciences
Omayma Missawi, Massimo Venditti, Tiziana Cappello, Nesrine Zitouni, Giuseppe DE. Marco, Iteb Boughattas, Noureddine Bousserrhine, Sabrina Belbekhouche, Sergio Minucci, Maria Maisano, Mohamed Banni
Summary: This study evaluates the impact of different environmental microplastics (MPs) collected from the south coast of the Mediterranean Sea on the metabolome and proteome of the marine polychaete Hediste diversicolor. The results show that MPs cause cytoskeleton damage and induce abnormal autophagy pathway in polychaetes. Metabolomics analysis reveals altered metabolic profiles in polychaetes treated with MPs, with elevated levels of amino acids, glucose, and other substances, and reduced concentration of aspartate.
ENVIRONMENTAL POLLUTION
(2022)
Review
Medicine, Research & Experimental
Weizhuo Lu, Zhiwu Chen, Jiyue Wen
Summary: Ischemic stroke is a common and serious disease, and neuroinflammation plays a crucial role in its progression. Microglia, astrocytes, and infiltrating immune cells are involved in the complicated neuroinflammation cascade, releasing different molecules that affect inflammation. Flavonoids, plant-specific compounds, have shown protective effects against cerebral ischemia injury by modulating the inflammatory responses.
BIOMEDICINE & PHARMACOTHERAPY
(2024)