Article
Cell Biology
Anne-Sophie Archambault, Francesco Tinto, Elizabeth Dumais, Volatiana Rakotoarivelo, Magdalena Kostrzewa, Pier-Luc Plante, Cyril Martin, Melissa Simard, Cristoforo Silvestri, Roxane Pouliot, Michel Laviolette, Louis-Philippe Boulet, Rosa Maria Vitale, Alessia Ligresti, Vincenzo Di Marzo, Nicolas Flamand
Summary: Endocannabinoids 2-arachidonoyl-glycerol and N-arachidonoyl-ethanolamine are lipids that regulate various physiological processes, including inflammation, and are being studied as potential anti-inflammatory agents. Human eosinophils and neutrophils have been found to metabolize 1-linoleoyl-glycerol (1-LG) and N-linoleoyl-ethanolamine (LEA) into novel endogenous 15-lipoxygenase metabolites 13-HODE-G and 13-HODE-EA, although the biological significance of these metabolites is not yet fully understood.
Article
Pharmacology & Pharmacy
Saskia Wedel, Tabea Osthues, Bela Zimmer, Carlo Angioni, Gerd Geisslinger, Marco Sisignano
Summary: This study found that the concentrations of oxidized linoleic acid metabolites, especially 9-HODE and 13-HODE, are significantly increased in inflamed paw tissue and the corresponding dorsal root ganglia in the sub-chronic phase of inflammation. Surprisingly, classical inflammatory lipid markers, such as prostaglandins, were at basal levels in this phase of inflammation. Moreover, the study revealed the cell type specific synthesis pathways of oxidized linoleic acid metabolites and showed that blocking the most elevated metabolites 9-HODE and 13-HODE in the sub-chronic phase of inflammation leads to a significant relief of mechanical and thermal hypersensitivity in vivo.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Malgorzata Szczuko, Kamila Pokorska-Niewiada, Lidia Kwiatkowska, Jolanta Nawrocka-Rutkowska, Iwona Szydlowska, Maciej Zietek
Summary: This study found an association between red blood cell potassium levels and fatty acids and inflammatory mediators in women with polycystic ovary syndrome (PCOS). Saturated fatty acids were found to reduce potassium influx into the cell and exacerbate inflammatory responses. Therefore, improving potassium flow inside the cell is crucial for the treatment of patients.
Article
Chemistry, Multidisciplinary
Ayako Watanabe, Kotaro Hama, Kohei Watanabe, Yuko Fujiwara, Kazuaki Yokoyama, Shigeo Murata, Ryo Takita
Summary: This study presents a protocol for the precise deuteration of straight-chain fatty acids, allowing for their persistent tracking and metabolic investigation. The research also identifies the acyltransferases involved in the metabolism of an oxidized fatty acid.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Engineering, Environmental
Kevin Crouvisier Urion, Rebeca Garcia, Aline Boussard, Laure Degrand, Wafa Guiga
Summary: This study is the first to investigate the role of gas-liquid oxygen transfer on the determination of kinetic parameters of an oxidase and reactor sizing for pure hydroperoxides production. It was found that the addition of a small amount of ethanol was suitable for simple linoleic acid handling. By reprocessing dissolved oxygen kinetics, accurate reaction rates were determined, showing consistency with hydroperoxide production rates.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Zhichao Zhang, Shiva Emami, Marie Hennebelle, Rhianna K. Morgan, Larry A. Lerno, Carolyn M. Slupsky, Pamela J. Lein, Ameer Y. Taha
Summary: The study showed that dietary 13-HODE can be absorbed and incorporated into peripheral tissues from esterified plasma lipid pools. Unesterified d4-13-HODE was rapidly eliminated from plasma with a half-life of 1 min following IV injection. Esterified tracer preferentially incorporated into liver, adipose, and heart compared to unesterified d4-13-HODE.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Nutrition & Dietetics
Won-Shik Choi, Xia Xu, Susan Goruk, Yixiong Wang, Samir Patel, Michael Chow, Catherine J. Field, Roseline Godbout
Summary: GBM neural stem-like cells express high levels of FABP7, and increasing DHA content can reduce their migration ability, potentially offering therapeutic value.
Article
Biochemistry & Molecular Biology
Francesco Tinto, Anne-Sophie Archambault, Elizabeth Dumais, Volatiana Rakotoarivelo, Magdalena Kostrzewa, Cyril Martin, Pier-Luc Plante, Yves Desjardins, Melissa Simard, Roxane Pouliot, Luciano De Petrocellis, Alessia Ligresti, Vincenzo Di Marzo, Nicolas Flamand
Summary: N-Arachidonoyl-ethanolamine (AEA) is an endocannabinoid mimicking the effects of Delta(9)-tetrahydrocannabinol, affecting brain functions, appetite, pain, and inflammation. Research on N-linoleoyl-ethanolamine (LAE) suggests that certain NAEs can be metabolized into 15-lipoxygenase-derived derivatives.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Chemistry, Applied
Ahmad Nasrollahzadeh, Samaneh Mollaei Tavani, Edris Arjeh, Seid Mahdi Jafari
Summary: Conjugated linoleic acid (CLA) has gained significant attention as a health-promoting compound. Microbial biosynthesis of CLA is a practical and controllable method for commercial production, and understanding the interplay of different factors is essential in optimizing its quantity and composition.
Article
Neurosciences
Hope Needham, Grace Torpey, Carlos C. Flores, Christopher J. Davis, William M. Vanderheyden, Jason R. Gerstner
Summary: Fatty acid binding protein 7 (FABP7) plays a crucial role in regulating wakefulness and sleep, inflammation, and neuroprotection through its interaction with different fatty acids.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Immunology
Na Zhao, Wenwen Zhu, Jia Wang, Weiwei Liu, Longdan Kang, Rui Yu, Beixing Liu
Summary: ILC2s play a key role in TNBC lung metastasis, potentially through the ILC2-derived IL-13-activated MDSC pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Microbiology
Nho-Eul Song, Na-Jeong Kim, Young-Hun Kim, Sang-Ho Baik
Summary: The study evaluated the probiotic properties of 10 strains of high CLA-producing lactic acid bacteria isolated from fermented seafood. Some of these strains produced significant amounts of CLA and demonstrated potential as probiotics with immune-modulating and antimicrobial activities in both cell and animal models.
Article
Biochemistry & Molecular Biology
Marco Fazzari, Steven R. Woodcock, Pascal Rowart, Karina Ricart, Jack R. Lancaster Jr, Rakesh Patel, Dario A. Vitturi, Bruce A. Freeman, Francisco J. Schopfer
Summary: This study identified nitro-nitrate ester-containing lipids as intermediates in unsaturated fatty acid nitration reactions in rat stomach, leading to the production of anti-inflammatory and tissue-protective signaling mediators. These observations provide new insight into the mechanisms of unsaturated fatty acid nitration and expand the scope of endogenous products resulting from nitrogen oxides metabolic reactions.
Article
Nutrition & Dietetics
Agnieszka Stawarska, Malgorzata Czerwonka, Malgorzata Jelinska, Iga Piasecka, Barbara Bobrowska-Korczak
Summary: The study found that zinc supplementation's impact on fatty acid metabolism in liver microsomes of breast cancer-induced rats depends on the form in which it is administered. Nano-Zn increased vaccenic acid content and decreased Δ6-desaturase activity, while micro-Zn increased cholesterol, COPs, and PGE2 content, and reduced concentrations of certain metabolites.
Article
Oncology
Yoshiteru Kagawa, Banlanjo Abdulaziz Umaru, Masayuki Kanamori, Ryo Zama, Subrata Kumar Shil, Hirofumi Miyazaki, Shuhei Kobayashi, Tunyanat Wannakul, Shuhan Yang, Teiji Tominaga, Yuji Owada
Summary: IDH1wt GB patients have significantly shorter survival than IDH1mut GB patients, making it crucial to study the biology of IDH1wt GB and develop effective molecular targeted therapies. FABP7 is highly expressed in IDH1wt glioma and plays a role in cell proliferation through epigenetic regulation of caveolin-1.
MOLECULAR ONCOLOGY
(2022)
Article
Oncology
Hamizah Sa'at, Yew-Kong Lee, Sook-Yee Yoon, Siu Wan Wong, Yin Ling Woo, Kristine Barlow-Stewart, Nur Aishah Mohd Taib
Summary: The study found that Malaysian BRCA mutation carriers need time to accept their mutation status before making a risk-reducing choice, understanding complex genetic information and multiple risk management options can be overwhelming, and obtaining more information and a second opinion can be challenging. Many felt the need for support from their spouse, family members, and religion to feel reassured with their decision.
Article
Genetics & Heredity
Pei Sze Ng, Rick A. C. M. Boonen, Eldarina Wijaya, Chan Eng Chong, Milan Sharma, Sabine Knaup, Shivaani Mariapun, Weang Kee Ho, Joanna Lim, Sook-Yee Yoon, Nur Aishah Mohd Taib, Mee Hoong See, Jingmei Li, Swee Ho Lim, Ern Yu Tan, Benita Kiat-Tee Tan, Su-Ming Tan, Veronique Kiat-Mien Tan, Rob Martinus van Dam, Kartini Rahmat, Cheng Har Yip, Sara Carvalho, Craig Luccarini, Caroline Baynes, Alison M. Dunning, Antonis Antoniou, Haico van Attikum, Douglas F. Easton, Mikael Hartman, Soo Hwang Teo
Summary: Rare protein-truncating variants (PTVs) in PALB2 confer increased risk of breast cancer in South-East Asian populations, with no association found between rare missense variants and breast cancer risk. Two novel rare missense variants were found to produce unstable proteins, leading to decreased DNA repair function. Despite genetic and lifestyle differences, the prevalence and risk of PALB2 PTVs in this population are similar to those reported in European populations.
JOURNAL OF MEDICAL GENETICS
(2022)
Review
Biochemistry & Molecular Biology
Boon Cheng Chew, Fong Fong Liew, Hsiao Wei Tan, Ivy Chung
Summary: This review examines the chemical and biological properties of exosomal and liposomal systems and discusses their potential biomedical applications and related challenges.
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Oncology
Siew Juan Kiew, Hazreen Abdul Majid, Nur Aishah Mohd Taib
Summary: The study found that participants had varying dietary practices, with some following dietary recommendations and others not. The social environment was crucial, as many women relied on family and friends for food choices, and individuals in charge of food preparation had to cater to family preferences. Additionally, individuals found it difficult to maintain healthy dietary changes during the acute survivorship phase due to a lack of health consciousness and limited access to healthy food. Importantly, there was a lack of dietary guidance from healthcare professionals, especially dietitians, in long-term survivorship care.
EUROPEAN JOURNAL OF CANCER CARE
(2022)
Article
Genetics & Heredity
Weang-Kee Ho, Mei-Chee Tai, Joe Dennis, Xiang Shu, Jingmei Li, Peh Joo Ho, Iona Y. Millwood, Kuang Lin, Yon-Ho Jee, Su-Hyun Lee, Nasim Mavaddat, Manjeet K. Bolla, Qin Wang, Kyriaki Michailidou, Jirong Long, Eldarina Azfar Wijaya, Tiara Hassan, Kartini Rahmat, Veronique Kiak Mien Tan, Benita Kiat Tee Tan, Su Ming Tan, Ern Yu Tan, Swee Ho Lim, Yu-Tang Gao, Ying Zheng, Daehee Kang, Ji-Yeob Choi, Wonshik Han, Han-Byoel Lee, Michiki Kubo, Yukinori Okada, Shinichi Namba, Sue K. Park, Sung-Won Kim, Chen-Yang Shen, Pei-Ei Wu, Boyoung Park, Kenneth R. Muir, Artitaya Lophatananon, Anna H. Wu, Chiu-Chen Tseng, Keitaro Matsuo, Hidemi Ito, Ava Kwong, Tsun L. Chan, Esther M. John, Allison W. Kurian, Motoki Iwasaki, Taiki Yamaji, Sun-Seog Kweon, Kristan J. Aronson, Rachel A. Murphy, Woon-Puay Koh, Chiea-Chuen Khor, Jian-Min Yuan, Rajkumar Dorajoo, Robin G. Walters, Zhengming Chen, Liming Li, Jun Lv, Keum-Ji Jung, Peter Kraft, Paul D. B. Pharoah, Alison M. Dunning, Jacques Simard, Xiao-Ou Shu, Cheng-Har Yip, Nur Aishah Mohd Taib, Antonis C. Antoniou, Wei Zheng, Mikael Hartman, Douglas F. Easton, Soo-Hwang Teo
Summary: This study developed breast cancer polygenic risk scores (PRSs) using data from Asian populations and evaluated their transferability across different ethnic subgroups. The results showed that the combined Asian and European PRSs had the best performance in stratifying the breast cancer risk for women of Asian ancestry.
GENETICS IN MEDICINE
(2022)
Article
Oncology
Peh Joo Ho, Alexis Jiaying Khng, Benita Kiat-Tee Tan, Ern Yu Tan, Su-Ming Tan, Veronique Kiak Mien Tan, Geok Hoon Lim, Kristan J. Aronson, Tsun L. Chan, Ji-Yeob Choi, Joe Dennis, Weang-Kee Ho, Ming-Feng Hou, Hidemi Ito, Motoki Iwasaki, Esther M. John, Daehee Kang, Sung-Won Kim, Allison W. Kurian, Ava Kwong, Artitaya Lophatananon, Keitaro Matsuo, Nur Aishah Mohd-Taib, Kenneth Muir, Rachel A. Murphy, Sue K. Park, Chen-Yang Shen, Xiao-Ou Shu, Soo Hwang Teo, Qin Wang, Taiki Yamaji, Wei Zheng, Manjeet K. Bolla, Alison M. Dunning, Douglas F. Easton, Paul D. P. Pharoah, Mikael Hartman, Jingmei Li
Summary: This study investigated the association between HLA genes and breast cancer susceptibility in Asian women. The results showed no significant association between any HLA allele and breast cancer risk in this population.
Article
Genetics & Heredity
Leila Dorling, Sara Carvalho, Jamie Allen, Michael T. Parsons, Cristina Fortuno, Anna Gonzalez-Neira, Stephan M. Heijl, Muriel A. Adank, Thomas U. Ahearn, Irene L. Andrulis, Paivi Auvinen, Heiko Becher, Matthias W. Beckmann, Sabine Behrens, Marina Bermisheva, Natalia V. Bogdanova, Stig E. Bojesen, Manjeet K. Bolla, Michael Bremer, Ignacio Briceno, Nicola J. Camp, Archie Campbell, Jose E. Castelao, Jenny Chang-Claude, Stephen J. Chanock, Georgia Chenevix-Trench, J. Margriet Collee, Kamila Czene, Joe Dennis, Thilo Dork, Mikael Eriksson, D. Gareth Evans, Peter A. Fasching, Jonine Figueroa, Henrik Flyger, Marike Gabrielson, Manuela Gago-Dominguez, Montserrat Garcia-Closas, Graham G. Giles, Gord Glendon, Pascal Guenel, Melanie Gundert, Andreas Hadjisavvas, Eric Hahnen, Per Hall, Ute Hamann, Elaine F. Harkness, Mikael Hartman, Frans B. L. Hogervorst, Antoinette Hollestelle, Reiner Hoppe, Anthony Howell, Anna Jakubowska, Audrey Jung, Elza Khusnutdinova, Sung-Won Kim, Yon-Dschun Ko, Vessela N. Kristensen, Inge M. M. Lakeman, Jingmei Li, Annika Lindblom, Maria A. Loizidou, Artitaya Lophatananon, Jan Lubinski, Craig Luccarini, Michael J. Madsen, Arto Mannermaa, Mehdi Manoochehri, Sara Margolin, Dimitrios Mavroudis, Roger L. Milne, Nur Aishah Mohd Taib, Kenneth Muir, Heli Nevanlinna, William G. Newman, Jan C. Oosterwijk, Sue K. Park, Paolo Peterlongo, Paolo Radice, Emmanouil Saloustros, Elinor J. Sawyer, Rita K. Schmutzler, Mitul Shah, Xueling Sim, Melissa C. Southey, Harald Surowy, Maija Suvanto, Ian Tomlinson, Diana Torres, Therese Truong, Christi J. van Asperen, Regina Waltes, Qin Wang, Xiaohong R. Yang, Paul D. P. Pharoah, Marjanka K. Schmidt, Javier Benitez, Bas Vroling, Alison M. Dunning, Soo Hwang Teo, Anders Kvist, Miguel de la Hoya, Peter Devilee, Amanda B. Spurdle, Maaike P. G. Vreeswijk, Douglas F. Easton
Summary: The study found that certain rare missense variants in ATM, BRCA1, BRCA2, and CHEK2 genes may increase the risk of developing breast cancer, while missense variants in the PALB2 gene are less likely to be associated with risk.
Article
Medicine, General & Internal
Wee-Ming Tan, Kean-Hooi Teoh, Mogana Ganggayah, Nur Taib, Hana Zaini, Sarinder Dhillon
Summary: This study aims to develop an automated natural language processing (NLP) algorithm to summarize the existing narrative breast pathology report to a narrower structured synoptic pathology report, in order to improve communication between pathologists and clinicians.
Review
Health Care Sciences & Services
Desiree Schliemann, Min Min Tan, Wilfred Mok Kok Hoe, Devi Mohan, Nur Aishah Taib, Michael Donnelly, Tin Tin Su
Summary: Cancer screening provision in resource-constrained settings is opportunistic, but the use of mHealth technologies can improve uptake and effectiveness. Interventions mostly focus on breast and cervical cancer awareness and screening uptake, with SMS text messages and telephone calls being commonly used mHealth technologies.
JOURNAL OF MEDICAL INTERNET RESEARCH
(2022)
Article
Multidisciplinary Sciences
Yi Lin Lee, Tania Islam, Mahmoud Danaee, Nur Aishah Taib
Summary: Regular physical activity after breast cancer diagnosis is associated with reduced mortality and better quality of life. However, this study found that breast cancer survivors in Malaysia had inadequate physical activity levels at diagnosis, which decreased over time. Malay ethnicity and being underweight were significantly associated with low physical activity levels. It is important to communicate about the benefits of physical activity on cancer outcomes and continue to encourage breast cancer survivors to be physically active throughout their survivorship, particularly among Malay ethnic group and underweight patients.
Article
Instruments & Instrumentation
Tze Hua Yeu, Intan Sofia Omar, S. F. Abdul Sani, Dharini Pathmanathan, Boon Tong Goh, Nithyialakshmi Ravindran, Ik Hui Teo, Yogeeta Gunasagran, Noor Azmi Mat Adenan, Ivy Chung, Amira Hajirah Abd Jamil
Summary: Obesity is strongly associated with increased risk and poorer prognosis of endometrial cancer (EC). Cancer-associated fibroblasts (CAFs) undergo metabolic reprogramming in response to obesity, leading to alterations in lipid metabolism that may contribute to EC progression. Raman spectroscopy is a reliable technique for detecting these metabolic changes in CAFs from EC patients with varying body mass index.
APPLIED SPECTROSCOPY
(2023)
Article
Chemistry, Physical
Ammar Alhasan, S. F. Abdul Sani, Hairul Anuar Tajuddin, Tammar Hussein Ali, Shameer Hisham, N. M. Ung, Nurul Amniyyah Azhar, Nur Akmarina B. M. Said, Amira Hajirah Abd Jamil, D. A. Bradley
Summary: This study reports the design and synthesis of an efficient contrast agent, I@MPA-Mn:ZnSe, which overcomes the limitations of conventional contrast agents and enhances imaging diagnosis. It shows good biocompatibility and low toxicity to cells, as well as enhanced CT contrast and fluorescence brightness, making it a promising bimodal contrast medium for further diagnostic applications.
RADIATION PHYSICS AND CHEMISTRY
(2023)
Article
Oncology
Noor Mastura Mohd Mujar, Maznah Dahlui, Nor Aina Emran, Imisairi Abdul Hadi, Yang Wai Yan, Sarojah Arulanantham, Chan Hooi Chea, Nur Aishah Mohd Taib
Summary: The study aimed to determine the pathway and time intervals for Breast Cancer Care (BCC) among women and develop a quality matrix framework. Through a retrospective cohort study in Malaysia, researchers identified key time points in the BCC journey and provided standardized language for monitoring and outcome research. The results can serve as quality indicators for community and hospital-based breast cancer programs.
JCO GLOBAL ONCOLOGY
(2022)
Article
Oncology
Chin Vern Song, Cheng-Har Yip, Nur Aishah Mohd Taib, Mee Hoong See, Li Ying Teoh, Evelyn M. Monninkhof, Marniza Saad, Cuno S. P. M. Uiterwaal, Nirmala Bhoo-Pathy
Summary: This study in Malaysia examined the adherence to treatment guidelines and its impact on survival rates in breast cancer patients. Results indicated that patients who followed the guidelines had higher survival rates, with older patients showing lower adherence.
JCO GLOBAL ONCOLOGY
(2022)
Article
Psychology, Clinical
Hamizah Sa'at, Yew-Kong Lee, Sook-Yee Yoon, Siu Wan Wong, Yin Ling Woo, Kristine Barlow-Stewart, Nur Aishah Mohd Taib
Summary: BRCA mutation carriers in Malaysia consider factors such as risk perception, self-preservation, motherhood obligation, and the importance of marriage when making decisions about risk-reducing salpingo-oophorectomy (RRSO). Some women fear the physical and emotional impact of the surgery, while others feel obligated to choose RRSO for their children's sake. The support and approval of their husbands are crucial, and women's decisions about RRSO evolve with changing priorities at different stages of life.
INTERNATIONAL JOURNAL OF BEHAVIORAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.