Article
Multidisciplinary Sciences
Andrea Piserchio, Kimberly J. Long, Luke S. Browning, Amanda L. Bohanon, Eta A. Isiorho, Kevin N. Dalby, Ranajeet Ghose
Summary: Protein translation, a high-energy process, requires robust regulation under energy-deprived conditions. The eEF-2K plays a crucial role in this regulation by suppressing translational elongation through phosphorylation of eEF-2. It has been suggested that eEF-2K's response to energy levels is mediated by AMPK and the mTOR pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Pharmacology & Pharmacy
Soghra Bagheri, Mahdie Rahban, Fatemeh Bostanian, Fatemeh Esmaeilzadeh, Arash Bagherabadi, Samaneh Zolghadri, Agata Stanek
Summary: Prostate cancer, when it spreads to the bone, is known as metastatic bone cancer. Currently, effective treatment options are still lacking for advanced/metastatic PC. However, understanding the molecular events that contribute to PC progression provides hope for the development of new treatment strategies. Protein kinases play crucial roles in the growth, proliferation, and metastases of prostatic tumors, making combinatorial therapy with PK inhibitors a potential treatment approach. Additionally, epigenetic pathways are also involved in PC, suggesting another potential combination treatment strategy.
Review
Nutrition & Dietetics
Qi Tang, Peng Tan, Ning Ma, Xi Ma
Summary: Threonine is an essential amino acid for animals and plays a crucial role in nutritional metabolism, macromolecular biosynthesis, and gut homeostasis. Supplementation of threonine can benefit energy metabolism and influence cell growth, immune system, and overall health in animals.
Article
Chemistry, Medicinal
Zhengnian Li, Ryosuke Ishida, Yan Liu, Jinhua Wang, Yina Li, Yang Gao, Jie Jiang, Jianwei Che, Jason M. Sheltzer, Matthew B. Robers, Tinghu Zhang, Kenneth D. Westover, Behnam Nabet, Nathanael S. Gray
Summary: This study describes a medicinal chemistry campaign to identify a CDK11 inhibitor. Through structure-guided medicinal chemistry modifications, a CDK11 probe ZNL-05-044 with improved selectivity was identified, which can arrest cell cycle and impact CDK11-dependent gene splicing in cells.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Zhi-Da Zhang, Bing Wen, Da-Jia Li, Dan-Xia Deng, Xiao-Dong Wu, Yin-Wei Cheng, Lian-Di Liao, Lin Long, Geng Dong, Li-Yan Xu, En-Min Li
Summary: In this study, we identified a novel phosphorylation site in Fascin and confirmed AKT2 as the kinase for this site. Phosphorylation of Fascin Threonine 403 suppresses the behavior of esophageal cancer cells, actin-bundling activity, and filopodia formation.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ting Lu, Yujie Lu, Lei Wang, Zhipei Liu, Shiyuan Miao, Yajie Tai, Binbin Yang
Summary: The Akt gene plays an important role in regulating female reproduction in Liposcelis entomophila by affecting the juvenile hormone signaling pathway and vitellogenesis.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Vinayak Walhekar, Chandrakant Bagul, Dileep Kumar, Amol Muthal, Garlapati Achaiah, Ravindra Kulkarni
Summary: Cytosolic PIM kinases play a crucial role in cancer progression, but there are currently no drugs targeting these kinases on the market. Many PIM kinase inhibitors have been developed in recent years, but have not passed clinical trials.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Article
Plant Sciences
Lijuan Chen, Jiahui Xiao, You Li, Yuxiao Song, Jun Liu, Qi Zhou, Ting Sun, Hong-Bin Wang, Bing Liu
Summary: Plant diseases caused by fungal pathogens can be regulated through jasmonate response and chitin signaling, specifically by the Raf-like MAPKKKs STY8, STY17, and STY46, as discovered in this study. Interaction with MKK7, a MAPKK component, also plays a role in resistance to Botrytis cinerea and chitin signaling. The accumulation of MKK7 is negatively affected by the homologs of STY8, STY17, and STY46, which have opposite effects on defense against B. cinerea.
Article
Oncology
Laura Bownes, Adele P. Williams, Raoud Marayati, Colin H. Quinn, Sara C. Hutchins, Jerry E. Stewart, Trung Vu, Juliet L. Easlick, Elizabeth Mroczek-Musulman, David K. Crossman, Joshua C. Anderson, Christopher D. Willey, Pran K. Datta, Elizabeth A. Beierle
Summary: Neuroblastoma, an aggressive childhood tumor, continues to have a poor prognosis with less than 50% five-year survival rate. The study shows that STRAP, a protein associated with tumor growth, plays a crucial role in neuroblastoma development and stemness maintenance.
Article
Microbiology
Qiao Hu, Lun Yao, Xia Liao, Liang-Sheng Zhang, Hao-Tian Li, Ting-Ting Li, Qing-Gen Jiang, Mei-Fang Tan, Lu Li, Roger R. Draheim, Qi Huang, Rui Zhou
Summary: A comparative study on STK/STP in Streptococcus suis revealed that STK plays a more critical role in cell division, energy metabolism, and protein translation, while STP primarily impacts bacterial growth. Deletion of stk or stp had significant effects on protein expression, with more virulence factors downregulated in increment stk strains. Overall, both STP and STK are essential regulatory proteins for S. suis, with STK playing a more prominent role in growth, cell division, and pathogenesis.
Article
Pediatrics
Laura Bownes, Raoud Marayati, Colin H. Quinn, Sara C. Hutchins, Jerry E. Stewart, Joshua C. Anderson, Christopher D. Willey, Pran K. Datta, Elizabeth A. Beierle
Summary: The study found that increased expression of STRAP in neuroblastoma patients is associated with unfavorable tumor characteristics, and that overexpression of STRAP leads to increased proliferation and growth of neuroblastoma cells, activating the FAK signaling pathway.
JOURNAL OF PEDIATRIC SURGERY
(2022)
Article
Biochemistry & Molecular Biology
Eva Louise Steinmetz, Denise Nicole Dewald, Uwe Walldorf
Summary: The study identified Hipk as a kinase that phosphorylates Yki, mapping several phosphorylation sites of Yki. These sites potentially provide additional regulation for Yki in vivo, with transgenic flies exhibiting notable phenotypes. Hipk is therefore an additional upstream regulator of Yki working in conjunction with Wts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Chen Zhang, Maoyu Wang, Yidie Ying, Fang Meng, Hongliang Gao, Shuxiong Zeng, Yasheng Zhu, Anwei Liu, Zhensheng Zhang, Chuanliang Xu
Summary: KIF4A is highly expressed in urothelial bladder carcinoma (UBC) and is associated with poor prognosis. Inhibition of KIF4A suppresses proliferation, migration, and invasion of UBC cells while promoting apoptosis, possibly through dephosphorylation of AKT, changes in EMT-related genes, and interaction with YAP1.
Review
Immunology
Jie-Jie Xu, Hai-Di Li, Xiao-Sa Du, Juan-Juan Li, Xiao-Ming Meng, Cheng Huang, Jun Li
Summary: PSTPIP2, a member of the F-BAR domain family, plays important roles in immune and inflammatory processes, making it a potential therapeutic target for AIDs. While it has been found that proteins like PTP-PEST, SHIP1, and CSK can bind to PSTPIP2 and inhibit AIDs development, the exact mechanisms of PSTPIP2's function remain to be fully elucidated.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mitesh Patel, Abdulrahman S. Bazaid, Esam I. Azhar, Hattan S. Gattan, Naif K. Binsaleh, Mirav Patel, Malvi Surti, Mohd Adnan
Summary: Given the rapid spread of the monkeypox virus and the increasing number of cases, it is urgent to develop effective drugs to combat the infection. This study focused on the 3D structure analysis of monkeypox virus Ser/Thr kinase and TK using molecular modeling techniques. Potential anti-viral phytochemicals were screened and selected based on their physicochemical properties and binding affinity towards Ser/Thr kinase and TK through molecular docking. Thalimonine and galanthamine were identified as promising compounds with strong binding affinity towards Ser/Thr kinase and TK.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Biochemistry & Molecular Biology
Polyxeni Bozatzi, Gopal P. Sapkota
BIOCHEMICAL SOCIETY TRANSACTIONS
(2018)
Review
Biochemistry & Molecular Biology
Sascha Roth, Luke J. Fulcher, Gopal P. Sapkota
CELLULAR AND MOLECULAR LIFE SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Luke J. Fulcher, Zhengcheng He, Lin Mei, Thomas J. Macartney, Nicola T. Wood, Alan R. Prescott, Arlene J. Whigham, Joby Varghese, Robert Gourlay, Graeme Ball, Rosemary Clarke, David G. Campbell, Christopher A. Maxwell, Gopal P. Sapkota
Review
Cell Biology
Luke J. Fulcher, Gopal P. Sapkota
Article
Cell Biology
Luke D. Hutchinson, Nicola J. Darling, Stephanos Nicolaou, Ilaria Gori, Daniel R. Squair, Philip Cohen, Caroline S. Hill, Gopal P. Sapkota
CELL DEATH & DISEASE
(2020)
Article
Cell Biology
Theresa Tachie-Menson, Ana Gazquez-Gutierrez, Luke J. Fulcher, Thomas J. Macartney, Nicola T. Wood, Joby Varghese, Robert Gourlay, Renata F. Soares, Gopal P. Sapkota
CELLULAR SIGNALLING
(2020)
Article
Biology
Karen Dunbar, Thomas J. Macartney, Gopal P. Sapkota
Summary: IMiDs bind to CRBN to recruit and degrade specific proteins like FAM83F, which interacts with CK1 alpha. The efficiency of target protein degradation by IMiDs depends on the nature of the multiprotein complex the protein is part of, as shown by the protective role of FAM83G on CK1 alpha degradation.
LIFE SCIENCE ALLIANCE
(2021)
Article
Biology
Karen Dunbar, Rebecca A. Jones, Kevin Dingwell, Thomas J. Macartney, James C. Smith, Gopal P. Sapkota
Summary: FAM83F protein interacts with CK1 alpha at the plasma membrane to activate Wnt signaling, leading to axis duplication in Xenopus embryos, and its effects on Wnt signaling are mediated through farnesylation at the plasma membrane.
LIFE SCIENCE ALLIANCE
(2021)
Article
Biochemical Research Methods
Peter Stacey, Hannah Lithgow, Xiao Lewell, Agnieszka Konopacka, Stephen Besley, Georgina Green, Ryan Whatling, Robert Law, Sascha Roeth, Gopal P. Sapkota, Ian E. D. Smith, Glenn A. Burley, John Harling, Andrew B. Benowitz, Markus A. Queisser, Marcel Muelbaier
Summary: A novel phenotypic screening approach was utilized to identify E3 ligase binders and generate functional HaloPROTACs for the degradation of fusion proteins in living cells. This method offers an important tool for further development of PROTACs.
Article
Biochemistry & Molecular Biology
Luke M. Simpson, Luke J. Fulcher, Gajanan Sathe, Abigail Brewer, Jin-Feng Zhao, Daniel R. Squair, Jennifer Crooks, Melanie Wightman, Nicola T. Wood, Robert Gourlay, Joby Varghese, Renata F. Soares, Gopal P. Sapkota
Summary: Reversible protein phosphorylation is crucial for controlling protein function and intracellular signaling. This study presents the development and application of the AdPhosphatase system, which allows targeted dephosphorylation of specific phospho-substrates. By using antigen-stabilized anti-GFP nanobody conjugated with Protein Phosphatase 1 or 2A catalytic subunits, the researchers successfully achieved specific dephosphorylation of two phospho-proteins, FAM83D and ULK1, with exquisite specificity. AdPhosphatase provides a new modality for potential drug discovery approaches by altering the phospho-status and function of target proteins.
CELL CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Luke M. Simpson, Lorraine Glennie, Abigail Brewer, Jin-Feng Zhao, Jennifer Crooks, Natalia Shpiro, Gopal P. Sapkota
Summary: This study investigates whether the subcellular context of a protein of interest (POI) affects the efficacy of PROTAC-mediated protein degradation. The results suggest that the subcellular localization of the POI can influence the effectiveness of PROTAC-induced POI degradation.
CELL CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sascha Roth, Thomas J. Macartney, Agnieszka Konopacka, Kwok-Ho Chan, Houjiang Zhou, Markus A. Queisser, Gopal P. Sapkota
CELL CHEMICAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Luke M. Simpson, Thomas J. Macartney, Alice Nardin, Luke J. Fulcher, Sascha Roth, Andrea Testa, Chiara Maniaci, Alessio Ciulli, Ian G. Ganley, Gopal P. Sapkota
CELL CHEMICAL BIOLOGY
(2020)
Meeting Abstract
Oncology
Sascha O. M. Roth, Thomas J. Macartney, Agnieszka Konopacka, Markus Queisser, Gopal P. Sapkota
MOLECULAR CANCER RESEARCH
(2020)