Article
Multidisciplinary Sciences
Mariana A. Branco, Tiago P. Dias, Joaquim M. S. Cabral, Perpetua Pinto-do-O, Maria Margarida Diogo
Summary: In this study, a self-organized human multilineage organoid was developed to recreate the co-emergence of pro-epicardium, septum transversum mesenchyme, and liver bud. The impact of WNT, BMP, and retinoic acid signaling modulation on the specification of multilineage organoids was investigated. Co-culturing these organoids with cardiomyocyte aggregates resulted in the generation of a self-organized heart organoid with an epicardium-like layer surrounding a myocardium-like tissue. These heart organoids recapitulates the impact of epicardial cells on promoting cardiomyocyte proliferation and maturation. This study provides valuable insights into the myocardium-epicardium interaction during heart organogenesis in both healthy and diseased settings.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Francesca Santilli, Jessica Fabrizi, Fanny Pulcini, Costantino Santacroce, Maurizio Sorice, Simona Delle Monache, Vincenzo Mattei
Summary: This review critically analyzes the role of gangliosides in the differentiation of mesenchymal stem cells and examines their potential use as specific cell surface markers.
Review
Cardiac & Cardiovascular Systems
Wai Hoe Ng, Barbie Varghese, Hongpeng Jia, Xi Ren
Summary: Studies have shown that signals from adjacent endodermal tissues play a crucial role in coordinating proper cardiac morphogenesis. In vitro models like cardiac organoids have potential, but cannot capture the complex communication between the heart and endodermal organs. Recent reports of multilineage organoids combining cardiac and endodermal derivatives have shed light on their morphogenesis and shared signaling requirements. These organoids offer insights into human development and could be used for regenerative interventions, disease investigation, and drug testing.
CIRCULATION RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Maria Gemma Velasco, Katy Satue, Deborah Chicharro, Emma Martins, Marta Torres-Torrillas, Pau Pelaez, Laura Miguel-Pastor, Ayla Del Romero, Elena Damia, Belen Cuervo, Jose Maria Carrillo, Ramon Cugat, Joaquin Jesus Sopena, Monica Rubio
Summary: In recent years, studies on Muse cells have highlighted their pluripotency, high stress tolerance, self-renewal capacity, DNA repair ability, and non-tumoral nature. Their isolation from adult tissues presents an ethical advantage over embryonic stem cells. Some studies have reported clinical applications of Muse cells in treating diseases such as aortic aneurysm, chondral injuries, and acute myocardial infarction in various animal models and humans. This review provides an overview of Muse cell characterization, biological characteristics, comparison with mesenchymal stem cells, and their therapeutic contributions to different diseases.
Article
Biochemistry & Molecular Biology
Shiva Pratap Singh, Suresh Dinkar Kharche, Manisha Pathak, Ravi Ranjan, Yogesh Kumar Soni, Manoj Kumar Singh, Ramasamy Pourouchottamane, Manmohan Singh Chauhan
Summary: Culturing caprine male germline stem cells under hypoxia enhances growth characteristics and stemness, but does not affect multilineage differentiation.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Intan Rosalina Suhito, Jin Won Kim, Kyeong-Mo Koo, Sun Ah Nam, Yong Kyun Kim, Tae-Hyung Kim
Summary: A new method is proposed for the real-time assessment of kidney organoids, allowing non-destructive evaluation of differentiation and maturation levels. This method utilizes electrochemical signals to differentiate kidney cells from off-target cells, providing a promising tool for organoid research.
Article
Cell & Tissue Engineering
Hyo Jin Kim, Gyeongmin Kim, Kyun Yoo Chi, Hyemin Kim, Yu Jin Jang, Seongyea Jo, Jihun Lee, Youngseok Lee, Dong-Hun Woo, Choongseong Han, Sang Kyum Kim, Han-Jin Park, Jong-Hoon Kim
Summary: Multilineage liver organoids (mLOs) generated from human pluripotent stem cells (hPSCs) can recapitulate the interactions between parenchymal and non-parenchymal cells, offering a precise in vitro model for liver diseases. This study demonstrates that the assembly of hepatic endoderm, hepatic stellate cell-like cells (HscLCs), and endothelial cells derived from hPSCs can generate mLOs with luminal blood vessels and bile ducts. Notch signaling modulation plays a critical role in the formation of both bile ducts and vasculature in mLOs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Reproductive Biology
Lina Castelo Branco Motta, Vitoria Mattos Pereira, Priscilla Avelino Ferreira Pinto, Celina Almeida Furlanetto Mancanares, Naira Caroline Godoi Pieri, Vanessa Cristina de Oliveira, Paulo Fantinato-Neto, Carlos Eduardo Ambrosio
Summary: Three-dimensional culture of stem cells derived from the canine yolk sac can develop artificial models similar to intestinal organs, providing accurate experimental data for cell therapy and drug testing, as well as serving as a study model for host interaction. The differentiation potential of yolk sac stem cells into multiple cell types is significant in basic and scientific biology research.
Article
Medicine, Research & Experimental
Zhichao Ni, Lingxia Lyu, Hui Gong, Luping Du, Zuoshi Wen, Hua Jiang, Hao Yang, Yanhua Hu, Bohuan Zhang, Qingbo Xu, Xiaogang Guo, Ting Chen
Summary: This study aimed to investigate the cellular sources that reshape vein grafts. By analyzing transcriptomics data and constructing inducible lineage-tracing mouse models, the researchers demonstrated that Sca-1(+) cells played important roles in vein grafts and could serve as progenitors for multilineage commitment. They also found that recipient Sca-1(+) cells dominated reendothelialization and the formation of adventitial microvessels, while bone-marrow-derived Sca-1(+) cells differentiated into inflammatory cells in vein grafts. Furthermore, non-bone-marrow-derived circulatory Sca-1(+) cells were vital for the formation of adventitial microvessels, while Sca-1(+) cells derived from local carotid arteries were the source of endothelium restoration.
Article
Cell Biology
Francesca Santilli, Jessica Fabrizi, Stefano Martellucci, Costantino Santacroce, Egidio Iorio, Maria Elena Pisanu, Mattea Chirico, Loreto Lancia, Fanny Pulcini, Valeria Manganelli, Maurizio Sorice, Simona Delle Monache, Vincenzo Mattei
Summary: This study analyzed the lipid composition of human Dental Pulp-Derived Stem Cells (DPSCs) and investigated the role of lipid rafts during multilineage differentiation. The results showed that mono-unsaturated fatty acids were the most representative species in the acyl chains of DPSCs. Moreover, disrupting lipid rafts prevented AKT activation and lineage-specific mRNA expression during multilineage differentiation, indicating the importance of lipid raft integrity in DPSCs differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Urology & Nephrology
Steicy Sobrino, Chrystelle Abdo, Benedicte Neven, Adeline Denis, Nathalie Gouge-Biebuyck, Emmanuel Clave, Soeli Charbonnier, Tifanie Blein, Camille Kergaravat, Marion Alcantara, Patrick Villarese, Romain Berthaud, Laurene Dehoux, Souha Albinni, Esma Karkeni, Chantal Lagresle-Peyrou, Marina Cavazzana, Remi Salomon, Isabelle Andre, Antoine Toubert, Vahid Asnafi, Capucine Picard, Stephane Blanche, Elizabeth Macintyre, Olivia Boyer, Emmanuell Six, Julien Zuber
Summary: Long-term multilineage hematopoietic donor chimerism can occur in patients who receive a transplanted solid organ with lymphoid tissues, such as the intestine or liver. However, there is currently no evidence for kidney-resident hematopoietic stem cells in any mammal species. In this study, we found that human kidney-derived hematopoietic stem cells can reside in the recipient's bone marrow and replace host counterparts, leading to full donor chimerism of both lymphoid and myeloid lineages.
KIDNEY INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Sachin H. Patel, Constantina Christodoulou, Caleb Weinreb, Qi Yu, Edroaldo Lummertz da Rocha, Brian J. Pepe-Mooney, Sarah Bowling, Li Li, Fernando G. Osorio, George Q. Daley, Fernando D. Camargo
Summary: In this study, in situ barcoding and classical fate mapping were used to assess the developmental and clonal origins of adult blood in mice. It was found that there is an early wave of progenitor specification independent of traditional HSCs, which predominately drives haematopoiesis in the young adult and has a decreasing yet lifelong contribution over time, serving as the predominant source of lymphoid output.
Article
Cell Biology
Takeshi Ouchi, Sohshi Morimura, Lukas E. Dow, Hiroyuki Miyoshi, Mark C. Udey
Summary: The study reveals the functional role of EpCAM in intestinal epithelial cells and its relation to intestinal disorders. Deletion of EpCAM leads to increased apoptosis, decreased cell proliferation, and disruptions in tight junctions and cell polarity in organoids and spheroids. Treatment with Rho-associated coiled-coil kinase (ROCK) inhibitors improves epithelial integrity in EpCAM-deficient cells.
Article
Biochemistry & Molecular Biology
Nicolette M. Johnson, Louis R. Parham, Jeeyoon Na, Keara E. Monaghan, Hannah M. Kolev, Alena Klochkova, Melissa S. Kim, Charles H. Danan, Zvi Cramer, Lauren A. Simon, Kaitlyn E. Naughton, Stephanie Adams-Tzivelekidis, Yuhua Tian, Patrick A. Williams, N. Adrian Leu, Simone Sidoli, Kelly A. Whelan, Ning Li, Christopher J. Lengner, Kathryn E. Hamilton
Summary: The intestinal epithelium shows a quick and efficient regenerative response to injury. Research suggests that high autophagic vesicle content in secretory cells predicts cell plasticity and resistance to DNA damaging injury, regardless of cell age.
Article
Biology
Wai Hoe Ng, Elizabeth K. Johnston, Jun Jie Tan, Jacqueline M. Bliley, Adam W. Feinberg, Donna B. Stolz, Ming Sun, Piyumi Wijesekara, Finn Hawkins, Darrell N. Kotton, Xi Ren
Summary: The interaction between the developing heart and lung is crucial for their proper formation. Researchers have developed a new human model using pluripotent stem cells to investigate this relationship, creating microtissues containing both heart and lung cells. The presence of developing heart cells was found to accelerate lung maturation in the microtissues, and the heart and lung tissues were observed to automatically separate from each other during later stages.
Article
Cell & Tissue Engineering
Philipp Woersdoerfer, Felicitas Bosen, Martina Gebhardt, Nicole Russ, Katrin Zimmermann, David Komla Kessie, Thileepan Sekaran, Angela Egert, Sueleyman Erguen, Hubert Schorle, Alexander Pfeifer, Frank Edenhofer, Klaus Willecke
Letter
Cell & Tissue Engineering
Philipp Woersdoerfer, Sueleyman Erguen
STEM CELL REVIEWS AND REPORTS
(2018)
Letter
Cell & Tissue Engineering
Philipp Woersdoerfer, Sueleyman Erguen
STEM CELL REVIEWS AND REPORTS
(2018)
Article
Cardiac & Cardiovascular Systems
Subba Rao Mekala, Philipp Woersdoerfer, Jochen Bauer, Olga Stoll, Nicole Wagner, Laurens Reeh, Kornelia Loew, Georg Eckner, Chee Keong Kwok, Erhard Wischmeyer, Mary Eleanor Dickinson, Harald Schulze, David Stegner, Ralf A. Benndorf, Frank Edenhofer, Verena Pfeiffer, Stefanie Kuerten, Stefan Frantz, Sueleyman Erguen
CIRCULATION RESEARCH
(2018)
Review
Cell Biology
Philipp Woersdoerfer, Nicole Wagner, Sueleyman Erguen
HISTOCHEMISTRY AND CELL BIOLOGY
(2018)
Article
Cell & Tissue Engineering
Marc Christian Thier, Oliver Hommerding, Jasper Panten, Roberta Pinna, Diego Garcia-Gonzalez, Thomas Berger, Philipp Woersdoefer, Yassen Assenov, Roberta Scognamiglio, Adriana Przybylla, Paul Kaschutnig, Lisa Becker, Michael D. Milsom, Anna Jauch, Jochen Utikal, Carl Herrmann, Hannah Monyer, Frank Edenhofer, Andreas Trumpp
Article
Cell Biology
Florian Kleefeldt, Heike Boemmel, Britta Broede, Michael Thomsen, Verena Pfeiffer, Philipp Woersdoerfer, Srikanth Karnati, Nicole Wagner, Uwe Rueckschloss, Sueleyman Erguen
Article
Multidisciplinary Sciences
Philipp Woersdoerfer, Nahide Dalda, Anna Kern, Sarah Krueger, Nicole Wagner, Chee Keong Kwok, Erik Henke, Suleyman Erguen
SCIENTIFIC REPORTS
(2019)
Review
Clinical Neurology
Philipp Woersdoerfer, I Takashi, Izumi Asahina, Yoshinori Sumita, Sueleyman Erguen
JOURNAL OF NEURAL TRANSMISSION
(2020)
Article
Clinical Neurology
I Takashi, Yuichiro Ueda, Philipp Worsdorfer, Yoshinori Sumita, Izumi Asahina, Sueleyman Erguen
JOURNAL OF NEURAL TRANSMISSION
(2020)
Article
Engineering, Biomedical
Leyla Dogan, Ruben Scheuring, Nicole Wagner, Yuichiro Ueda, Sven Schmidt, Philipp Woersdoerfer, Juergen Groll, Sueleyman Erguen
Summary: Post-fabrication formation of proper vasculature in bioprinting remains a challenge. Human induced pluripotent stem cell-derived mesodermal progenitor cells (hiMPCs) have shown the ability to form complex vessels in vitro, and demonstrate potential in printing vascular structures in vivo.
Article
Immunology
Fengming Liu, Kun Han, Robert Blair, Kornelia Kenst, Zhongnan Qin, Berin Upcin, Philipp Woersdoerfer, Cecily C. Midkiff, Joseph Mudd, Elizaveta Belyaeva, Nicholas S. Milligan, Tyler D. Rorison, Nicole Wagner, Jochen Bodem, Lars Doelken, Bertal H. Aktas, Richard S. Vander Heide, Xiao-Ming Yin, Jay K. Kolls, Chad J. Roy, Jay Rappaport, Sueleyman Erguen, Xuebin Qin
Summary: This study found that SARS-CoV-2 may directly infect mature vascular endothelial cells in vivo and ex vivo, potentially contributing to cardiovascular complications in SARS-CoV-2 infection, including multiple organ failure.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Cell & Tissue Engineering
Tobias Koeniger, Luisa Bell, Anika Mifka, Michael Enders, Valentin Hautnnann, Subba Rao Mekala, Philipp Kirchner, Arif B. Ekici, Christian Schulz, Philipp Woersdoerfer, Stine Mencl, Christoph Kleinschnitz, Sueleynnan Erguen, Stefanie Kuerten
Summary: This study reveals the presence of a heterogeneous population of myeloid progenitors in the leptomeninges of adult mice, capable of giving rise to various hematopoietic lineages. These progenitors are derived from adult hematopoietic stem cells and are continuously replaced through cell trafficking. Their occurrence is linked to the immunological state of the central nervous system and is decreased in the presence of neuroinflammation and ischemic stroke, suggesting possible roles in CNS surveillance and local immune cell production.
Article
Biochemical Research Methods
Philipp Woersdoerfer, Anna Rockel, Yvonne Alt, Anna Kern, Suleyman Erguen