Article
Cell & Tissue Engineering
Nathan T. Schomer, Ziyue Karen Jiang, Marit I. Lloyd, Hans Klingemann, Laurent Boissel
Summary: This study demonstrates that introducing the CCR7 receptor into t-haNK cells improves their homing towards lymph node chemokines, resulting in enhanced tumor control.
Review
Medicine, Research & Experimental
Junfeng Chu, Fengcai Gao, Meimei Yan, Shuang Zhao, Zheng Yan, Bian Shi, Yanyan Liu
Summary: This article summarizes the recent advances and clinical applications of natural killer (NK) cell immunotherapy, focusing on the biology and function of NK cells, different types of NK cell therapy, and clinical trials and future perspectives of NK cell therapy.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Mehmet Gunduz, Pinar Ataca Atilla, Erden Atilla
Summary: NK cell-mediated adoptive immunotherapy has gained attention in hematology field due to the advancing understanding of NK cell receptor structure, biology and function. Recent technological advancements in gene editing and CARs have enabled the generation of genetically modified NK cells to enhance anti-tumor efficacy.
Article
Oncology
Hae-Yun Jung, Dae-Keum Lee, Minwook Lee, Seung Hee Choi, Joo Dong Park, Eun-Su Ko, Jongwon Lee, Kyung-Soon Park, Hae-Yun Jung
Summary: Triple-negative breast cancer (TNBC) is a challenging subtype due to its aggressive behavior and limited treatment options. Immunotherapy has shown promise in prolonging survival, but its efficacy in solid cancers is limited. This study demonstrates that targeting the ELK3 gene can recruit immune cells, including NK cells, by increasing the expression of the chemokine CXCL16. Low ELK3 expression and high CXCL16 expression are associated with better prognosis and increased expression of NK cell-related genes. Targeting the ELK3 gene could be a strategy to enhance immunotherapy efficacy in TNBC.
Article
Biochemistry & Molecular Biology
Xianwu Wang, Xi Luo, Yunpeng Tian, Ting Wu, Jian Weng, Zhu Li, Feng Ye, Xuefei Huang
Summary: The study demonstrates that functionalizing NK cells with cetuximab through metabolic glycoengineering enhances tumor targeting abilities and improves anticancer efficacy against KRAS mutant colon cancer cells.
ACS CHEMICAL BIOLOGY
(2021)
Review
Immunology
Kari A. Shaver, Tayler J. Croom-Perez, Alicja J. Copik
Summary: This review highlights the crucial role of NK cells in cancer immunotherapy, emphasizing their importance in priming the immune system, activating the adaptive immune response, and improving treatment success rates. However, compromised NK cell immunity in many patients reduces the likelihood of successful outcomes from immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Federica Portale, Diletta Di Mitri
Summary: Natural killer cells (NK) are innate lymphocytes with the ability to recognize and kill cancer cells, making them a promising candidate for cancer treatment. However, cancer can impair the function of NK cells, limiting their effectiveness in cell therapies. Extensive research has been conducted to understand the mechanisms of NK cell dysfunction in cancer and to find ways to enhance their anti-tumor function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Xin Chen, Lei Jiang, Xuesong Liu
Summary: This review discusses the contributions of natural killer (NK) cells in cancer treatment, explores the current strategies at harnessing NK cells for cancer treatment, and highlights the importance of NK cell-mediated therapies in the next wave of cancer immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Cell & Tissue Engineering
Faroogh Marofi, Heshu Sulaiman Rahman, Lakshmi Thangavelu, Aleksey Dorofeev, Favian Bayas-Morejon, Naghmeh Shirafkan, Navid Shomali, Max Stanley Chartrand, Mostafa Jarahian, Ghasem Vahedi, Rebar N. Mohammed, Somayeh Shahrokh, Morteza Akbari, Farhad Motavalli Khiavi
Summary: CAR-T cell immunotherapy has shown promising therapeutic results in treating cancers and infectious diseases, but it also brings some considerable drawbacks, leading to the exploration of utilizing NK cells as an alternative source of immune effector cells for CAR-engineering.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Chemistry, Multidisciplinary
Bohwa Han, Yeonju Song, Jeehun Park, Junsang Doh
Summary: This review discusses the use of nanomaterials to address the limitations of CAR-T cell therapy, including methods to improve expansion, gene delivery, and enhance antitumor functions. Understanding the biological principles behind various nanomaterials is crucial for their application in ex vivo engineering of T/NK cells.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Cell Biology
Eilis Russell, Melissa J. Conroy, Martin P. Barr
Summary: Lung cancer, with NSCLC as the majority, is the leading cause of cancer-related deaths worldwide. Early lung cancer often presents with no symptoms, leading to late-stage diagnosis. Despite current treatments, many patients experience tumor relapse or develop therapeutic resistance, highlighting the need for more effective therapies.
Article
Biochemistry & Molecular Biology
Chantal Reina-Ortiz, M. Pilar Mozas, David Ovelleiro, Fei Gao, Martin Villalba, Alberto Anel
Summary: Therapies based on allogenic Natural Killer (NK) cells, specifically expanded and activated NK (eNK) cells, have shown significant cytotoxicity against hematological cancers. This study aims to understand the changes that occur in healthy NK cells during the expansion process in order to develop a more potent eNK-based therapy. MicroRNA (miRNA) expression analysis revealed changes in the expression of 64 miRNAs, highlighting the up-regulation of miRs-146a, -124, -34a, and -10a, which play a key role in cell survival, and the down-regulation of miRs-199a, -223, and -340, associated with increased NK cell cytotoxicity. Validation experiments confirmed the functional importance of these miRNAs and demonstrated the wide variety of changes in eNK cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Jeremiah L. Oyer, Tayler J. Croom-Perez, Thomas A. Dieffenthaller, Liza D. Robles-Carillo, Sarah B. Gitto, Deborah A. Altomare, Alicja J. Copik
Summary: This study evaluated the impact of cryopreservation on PM21-NK cells and found that PM21-NK cells can be efficiently cryopreserved and recovered without substantial loss of viability or activity, while retaining effector function both in vitro and in vivo.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Giuseppe La Verde, Valeria Artiola, Valeria Panzetta, Mariagabriella Pugliese, Paolo A. Netti, Sabato Fusco
Summary: The cytoskeleton plays a crucial role in various biological processes such as adhesion, motility, and intracellular transport. Alterations in its components are linked to diseases like cancer, affecting cell properties and invasiveness. Understanding the effects of ionizing radiations on the cytoskeleton can aid in developing diagnostic tools and improving cancer treatments.
Article
Immunology
Nannan Yang, Kristin Lode, Rodrigo Berzaghi, Ashraful Islam, Inigo Martinez-Zubiaurre, Turid Hellevik
Summary: Recent studies show that cancer-associated fibroblasts (CAFs) inhibit NK cell activation by reducing proliferation rates, cytotoxic capacity, degranulation extent, and stimulatory receptor expression, while enhancing inhibitory receptor expression. Ionizing radiation does not reverse CAF-mediated immunosuppressive effects over NK cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Waqas Muhammad Usman, Tin Chanh Pham, Yuk Yan Kwok, Luyen Tien Vu, Victor Ma, Boya Peng, Yuen San Chan, Likun Wei, Siew Mei Chin, Ajijur Azad, Alex Bai-Liang He, Anskar Y. H. Leung, Mengsu Yang, Ng Shyh-Chang, William C. Cho, Jiahai Shi, Minh T. N. Le
NATURE COMMUNICATIONS
(2018)
Article
Oncology
Kyung Won Kim, Jae-UK Jeong, Kyung-Hwa Lee, Tung Nguyen Thanh Uong, Joon Haeng Rhee, Sung-Ja Ahn, Sang-Ki Kim, Duck Cho, Huy Phuoc Quang Nguyen, Chanh Tin Pham, Mee Sun Yoon
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2020)
Article
Oncology
Marco Pirisinu, Tin Chanh Pham, Daniel Xin Zhang, Tran Nguyen Hong, Lap Thi Nguyen, Minh T. N. Le
Summary: This article discusses the potential application of extracellular vesicles (EVs) as anticancer therapeutic agents. It highlights EVs' role in cancer immunotherapy and their advantages as delivery systems for anticancer agents. The article describes the biology of EVs, methods for their isolation, characterization, and functional analysis, as well as emerging challenges in EV imaging and drug loading strategies. It emphasizes the importance of standardized protocols and proper analytical approaches for rigorous and reproducible EV research.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Cell Biology
Tin Chanh Pham, Migara Kavishka Jayasinghe, Thach Tuan Pham, Yuqi Yang, Likun Wei, Waqas Muhammad Usman, Huan Chen, Marco Pirisinu, Jinhua Gong, Seongkyeol Kim, Boya Peng, Weixi Wang, Charlene Chan, Victor Ma, Nhung T. H. Nguyen, Dennis Kappei, Xuan-Hung Nguyen, William C. Cho, Jiahai Shi, Minh T. N. Le
Summary: The study presents a novel method for covalently conjugating EVs with targeting moieties using protein ligases, enabling specific delivery of therapeutic payloads to target cells without the need for genetic and chemical modifications. This approach shows promise for enhancing drug efficacy in cancer therapy by targeting specific receptors on cancer cells.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Biotechnology & Applied Microbiology
Boya Peng, Yuqi Yang, Zhiyuan Wu, Rachel Tan, Thach Tuan Pham, Eric Yew Meng Yeo, Marco Pirisinu, Migara Kavishka Jayasinghe, Tin Chanh Pham, Kun Liang, Ng Shyh-Chang, Minh T. N. Le
Summary: Cancer cachexia is a syndrome characterized by muscle loss, and inhibiting myostatin has shown promise in preserving muscle mass. However, existing methods of myostatin inhibition have had limited effectiveness and side effects. In this study, a novel method using red blood cell-derived extracellular vesicles to deliver Mstn siRNA to skeletal muscles was tested in a mouse model of cancer cachexia. The results showed that this delivery method was safe, non-inflammatory, and led to increased muscle growth and prevention of cachexia.
Article
Medicine, Research & Experimental
Migara Kavishka Jayasinghe, Marco Pirisinu, Yuqi Yang, Boya Peng, Thach Tuan Pham, Chang Yu Lee, Melissa Tan, Luyen Tien Vu, Xuan T. T. Dang, Tin Chanh Pham, Huan Chen, Anskar Y. H. Leung, William C. Cho, Jiahai Shi, Minh T. N. Le
Summary: The use of extracellular vesicles (EVs) as drug delivery vectors has gained attention due to their ability to efficiently deliver therapeutic payloads to diseased cells. Surface functionalization methods have been developed to enhance the stability and biocompatibility of EVs, allowing for targeted delivery to cancer cells and improved metastasis suppression. Extensive testing has shown that engineered EVs are biocompatible and safe for repeated dose treatment.
Article
Cell Biology
Chanh Tin Pham, Xin Zhang, Austin Lam, Minh T. N. Le
