Article
Ecology
Tessa Camenzind, Kay Philipp Grenz, Johannes Lehmann, Matthias C. Rillig
Summary: The study revealed unexpected high flexibility in C:N and C:P values of saprobic fungi along nutrient supply gradients, questioning microbial homeostasis. Fungal N:P varied less due to simultaneous reductions in mycelial N and P contents. Internal recycling processes and reduced N and P uptake during mycelial growth may be more relevant than element storage.
Article
Agronomy
Dongwei Li, Yuhui Yang, Yulong Zhao, Guangli Tian, Mingsi Li, Husen Qiu, Xinguo Zhou
Summary: This study explores the influence of soil microbes on cotton growth. It shows that the diversity and composition of soil bacterial and fungal communities are significantly different between the seedling and flowering/boll-setting stages. The abundance of specific microbial taxa is correlated with cotton growth indices, mainly regulated by soil salinity and nitrate content.
Review
Biochemistry & Molecular Biology
Manjula Muthu Narayanan, Norhayati Ahmad, Pooja Shivanand, Faizah Metali
Summary: This article discusses the multifaceted stresses faced by plants, jeopardizing crop production. It also emphasizes the need to find alternative biocontrol agents for environmentally-friendly plant disease control.
Article
Engineering, Environmental
Miaomiao Li, Jun Yao, Geoffrey Sunahara, Robert Duran, Bang Liu, Ying Cao, Hao Li, Wancheng Pang, Houquan Liu, Shun Jiang, Junjie Zhu, Qinghua Zhang
Summary: There are scarce studies on the processes of concurrent bacterial and fungal community assembly that govern metal(loid)s biogeochemical cycles at smelters. This study systematically investigates the geochemical characteristics, co-occurrence patterns, and assembly mechanisms of bacterial and fungal communities in soils around an abandoned arsenic smelter. The results show that Acidobacteriota, Actinobacteriota, Chloroflexi, and Pseudomonadota are dominant in bacterial communities, while Ascomycota and Basidiomycota dominate fungal communities. The study also reveals the positive impact of certain metal(loid)s on bacteria and fungi through microbe-contaminant interactions.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Microbiology
Alejandro Ledezma-Villanueva, Tatiana Robledo-Mahon, Cinta Gomez-Silvan, Gabriela Angeles-De Paz, Clementina Pozo, Maximino Manzanera, Concepcion Calvo, Elisabet Aranda
Summary: Emerging and unregulated contaminants in soils can be treated through bioremediation using native microorganisms. Enrichment experiments have shown that certain fungi and bacteria have the capability to degrade pollutants, indicating their potential use in tailored bioremediation techniques.
Article
Microbiology
Junbo Peng, Xinghong Li, Yonghua Li, Wei Zhang, Ying Zhou, Jiye Yan
Summary: The LtScp1 protein in L. theobromae is highly expressed during the infectious stages and acts as a virulence factor to protect the fungus from degradation. It interacts with chitinase to interfere with its chitin-binding ability.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Ecology
Patrick Baudy, Jochen P. Zubrod, Marco Konschak, Sara Kolbenschlag, Annika Pollitt, Christiane Baschien, Ralf Schulz, Mirco Bundschuh
Summary: Fungi play a critical role in decomposing dead organic matter in ecosystems. Using biomolecular tools, researchers investigated fungal interactions in aquatic environments, finding that fungal diversity significantly impacts leaf colonization.
Review
Virology
Annegrete Visnapuu, Marie van der Gucht, Jeroen Wagemans, Rob Lavigne
Summary: Bacterial biofilms have defense mechanisms against phage predation, while phages have developed counter mechanisms to overcome the biofilm barrier. Research on these natural systems has inspired new antibiofilm design strategies and potential approaches to further explore novel antibiofilm mechanisms.
Article
Horticulture
Qing Wang, Ruiping Xu, Mengpei Guo, Nan Shen, Phiraphat Chuaoen, Kaixiao Qiu, Yinbing Bian, Yang Xiao
Summary: This study reported a new mycelial sluggish growth disease (MSGD) on Flammulina filiformis and elucidated the molecular mechanism of the edible mushroom in response to Pseudomonas migulae infection through transcriptome analysis.
SCIENTIA HORTICULTURAE
(2022)
Review
Biochemistry & Molecular Biology
Ali Mohamed Elyamine, Jie Kan, Shanshan Meng, Peng Tao, Hui Wang, Zhong Hu
Summary: Microbial biodegradation is an effective technology for remediating and controlling pollution by polycyclic aromatic hydrocarbons, involving aerobic and anaerobic degradation pathways by various bacteria and fungi. Understanding the key actors, steps, and mechanisms of pyrene degradation is crucial for addressing PAH pollution. Further research on anaerobic pyrene degradation is recommended to elucidate the degradation pathway and enhance biodegradation efficiency through microbial consortia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Nivedhitha Kabeerdass, Ahmed Al Otaibi, Manikandan Rajendran, Ayyar Manikandan, Heba A. Kashmery, Mohammed M. Rahman, P. Madhu, Anish Khan, Abdullah M. Asiri, Maghimaa Mathanmohun
Summary: The supernatant of soil-borne pathogen Bacillus mn14 was used as a catalyst for the synthesis of silver nanoparticles (AgNPs), which showed antibacterial and antifungal activities. The AgNPs demonstrated potential in inhibiting S. viridans and R. solani. Various characterization techniques confirmed the properties of the AgNPs, suggesting a promising approach for disease treatment and agricultural applications.
Article
Multidisciplinary Sciences
Sara Correa-Garcia, Vincenzo Corelli, Julien Tremblay, Jessica Ann Dozois, Eugenie Mukula, Armand Seguin, Etienne Yergeau
Summary: This study aimed to investigate the impact of soil faunal-microbial interaction complexity (SFMIC) on soil microbial communities and willow growth, particularly in the context of PAH contamination. The results showed that SFMIC had significant effects on the height and biomass of willows, as well as the structure and composition of bacterial, archaeal, and fungal communities. Certain fungal genera known to be willow symbionts during phytoremediation, such as Sphaerosporella, and bacterial phyla containing PAH degraders, such as Actinobacteriota, were significantly affected by SFMIC. However, the abundance and structure of PAH degraders were not clearly influenced by SFMIC treatments. Overall, the findings suggest that SFMIC can alter the outcomes of willow phytoremediation under the experimental conditions.
Article
Chemistry, Physical
Ross M. McBee, Matt Lucht, Nikita Mukhitov, Miles Richardson, Tarun Srinivasan, Dechuan Meng, Haorong Chen, Andrew Kaufman, Max Reitman, Christian Munck, Damen Schaak, Christopher Voigt, Harris H. Wang
Summary: The study presents a method to transform lignocellulosic waste into fungal-bacterial biocomposites for the production of moldable, foldable, and regenerative living structures. By analyzing the microbial composition and introducing engineered bacterial components, new biosynthetic and sensing-reporting capabilities can be developed.
Article
Microbiology
Hyun Kim, Yong-Hwan Lee
Summary: Seeds carry genetic information and microbial communities that influence plant vigor. Seed coats serve as microbial niches affecting the composition and diversity of bacterial and fungal communities. Microbial communities originating from seeds persist in the leaf, stem, and root endospheres throughout the plant's life cycle.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Olga A. Lastovetsky, Tancredi Caruso, Fiona P. Brennan, David P. Wall, Sean McMahon, Evelyn Doyle
Summary: This study provides empirical evidence of a selective and bi-directional relationship between AMF and bacterial communities co-inhabiting plant roots, explaining up to 36% of each other's variance. These findings contribute to a better understanding of the interaction between AMF and bacterial communities in plant root systems.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Materials Science, Biomaterials
Kyoka Komaba, Nobuhiko Nomura, Hiromasa Goto
Summary: Living organisms use signal messengers to respond to stimuli, and 3-oxo-C12-HSL is a messenger molecule that plays a role in bacterial colonies. In this study, a conductive polymer was successfully prepared by electrochemical polymerization and composite formation using the crystal form of 3-oxo-C12-HSL as a template. The achievement of electrochemical polymerization in the crystal structure of a QS material is significant in this research.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2022)
Article
Multidisciplinary Sciences
Sayoko Oiki, Takashi Yaguchi, Syun-ichi Urayama, Daisuke Hagiwara
Summary: This study investigated 99 strains of Penicillium isolated from various environments, including food, dead bodies, and clinical samples. The results showed that 14 and 20 strains displayed resistance to fludioxonil and iprodione, respectively, with 6 strains exhibiting multi-drug resistance to both fungicides. Some of these resistant strains had mutations in NikA, a histidine kinase involved in the mode of action of fludioxonil and iprodione.
Article
Biotechnology & Applied Microbiology
Shunsuke Masuo, Chisa Saga, Kurumi Usui, Yuma Sasakura, Yukie Kawasaki, Naoki Takaya
Summary: This study successfully produced raspberry ketone (RK) from simple carbon sources by genetically engineering the metabolism of Escherichia coli. The engineered E. coli produced high amounts of metabolic precursors and used overexpression of specific enzymes to increase RK production. The production system is inexpensive and does not rely on plant extraction, making it a significant contribution to the flavor and fragrance industries.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Akihiro Ninomiya, Syun-ichi Urayama, Daisuke Hagiwara
Summary: Co-culturing fungi with other microorganisms enhances the production of secondary metabolites. In this study, the activation of specific gene expressions in the co-culture system of two fungal species resulted in the production of different compounds compared to previous co-culture studies with bacteria. The molecular genetic mechanisms behind the biosynthesis of these compounds were demonstrated.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Editorial Material
Immunology
Daisuke Hagiwara, Sabine Fillinger, Dominique Sanglard
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Ayano Ikeda, Yuto Chiba, Misa Kuroki, Syun-ichi Urayama, Daisuke Hagiwara
Summary: This study established a method to remove mycoviruses from fungal host cells using antiviral drugs and compared their efficiency. The results showed that the specific viral species and antiviral drug played an important role in removing mycoviruses.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Yan-jie Zhao, Takashi Shirouzu, Yuto Chiba, Kentaro Hosaka, Hiromitsu Moriyama, Syun-ichi Urayama, Daisuke Hagiwara
Summary: In this study, viral screening of wild basidiomycota mushrooms was conducted using agarose gel electrophoresis and dsRNA sequencing. Seven isolates with virus-like bands were detected during initial screening, and five isolates were still found to have viruses after long-term storage. By using the FLDS method, seven viral genome sequences belonging to different families, including Partitiviridae, Curvu-laviridae, Endornaviridae, and Tulasviridae, were obtained. All viruses characterized in this study are novel species, greatly expanding our knowledge of the diversity of RNA viruses from environmental samples.
Article
Biotechnology & Applied Microbiology
Tatsuki Kunoh, Tatsuya Yamamoto, Erika Ono, Shinya Sugimoto, Kyosuke Takabe, Minoru Takeda, Andrew S. Utada, Nobuhiko Nomura
Summary: The regulation of cell chain elongation of filamentous bacteria via extracellular signals has been studied to prevent clogging of water distribution systems and filamentous bulking of activated sludge in industrial settings. The bacterium Leptothrix cholodnii generates cell chains encased in sheaths composed of woven nanofibrils. This study identified a novel glycosyltransferase, LthB, involved in nanofibril biosynthesis and demonstrated the functional differences between LthA and LthB in cell chain elongation and pellicle formation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Kohei Takahashi, Xiaojie Li, Tatsuki Kunoh, Ryo Nagasawa, Norio Takeshita, Andrew S. Utada
Summary: Microorganisms form communities in various environmental niches that are filled with micrometer-scale gaps and features. These microorganisms adapt to and are influenced by their physical environment. Conventional culture methods do not accurately mimic the complexity of natural micrometer-scale environments, hindering the study of their ecological behavior. Microfluidics has emerged as a valuable tool to study microorganisms, allowing the manipulation of micrometer-scale flows and real-time imaging. This review discusses insights into bacterial and fungal behavior obtained through microfluidics and explores its potential for further adoption.
MICROBES AND ENVIRONMENTS
(2023)
Article
Biotechnology & Applied Microbiology
Yuto Chiba, Akinori Yabuki, Yoshihiro Takaki, Takuro Nunoura, Syun-Ichi Urayama, Daisuke Hagiwara
Summary: The current knowledge on eukaryotic RNA viruses mainly focuses on animals, plants, and fungi, with limited understanding of the protist RNA virosphere. To uncover untapped RNA viral diversity, researchers screened RNA viruses from 30 marine protist isolates and discovered a novel RNA virus, named Haloplacidia narnavirus 1 (HpNV1). Phylogenetic analysis revealed that HpNV1 belongs to the family Narnaviridae. This study fills a gap in the distribution of narnaviruses and suggests their wide presence in Stramenopiles.
MICROBES AND ENVIRONMENTS
(2023)
Article
Multidisciplinary Sciences
Ayaka Itani, Shunsuke Masuo, Riho Yamamoto, Tomoko Serizawa, Yu Fukasawa, Naoki Takaya, Masatsugu Toyota, Shigeyuki Betsuyaku, Norio Takeshita
Summary: This paper visualized for the first time how calcium signaling is conducted inside the mycelial network in response to localized stimuli in the model fungus Aspergillus nidulans. The wavy propagation or blinking of the calcium signal inside the mycelium varies depending on the type and proximity of the stress. The mycelium showed growth delay only in the stressed areas, with reorganization of the actin cytoskeleton and membrane trafficking playing a role in the arrest and resumption of mycelial growth. These findings provide evidence of a decentralized response through locally activated calcium signaling in the mycelial network lacking a brain or nervous system.
Review
Biotechnology & Applied Microbiology
Syun-ichi Urayama, Yoshihiro Takaki, Yuto Chiba, Yanjie Zhao, Misa Kuroki, Daisuke Hagiwara, Takuro Nunoura
Summary: This review summarizes the host-virus relationships of eukaryotic microbial RNA viruses. By analyzing the classification and environmental distribution of different virus families, it is found that persistent-type viruses play an important role in viral diversity and host relationships.
MICROBES AND ENVIRONMENTS
(2022)
Article
Biotechnology & Applied Microbiology
Yukino Mizutani, Yuto Chiba, Syun-ichi Urayama, Yuji Tomaru, Daisuke Hagiwara, Kei Kimura
Summary: This study found that persistent RNA viruses are widespread in red macroalgae and nori sheets, and they may coexist with these plants for a long time. This study is also the first to report the presence of persistent RNA viruses in nori sheets and their raw materials.
MICROBES AND ENVIRONMENTS
(2022)
Article
Biochemistry & Molecular Biology
Hajime Minakawa, Shunsuke Masuo, Kenji Takada, Takuya Kumakura, Nozomi Katsuki, Tatsuo Kaneko, Naoki Takaya
Summary: In this study, 4APEA was produced through fermentation using genetically engineered Escherichia coli with the highest reported yield to date (7.2%) within 72 h. The fermented 4APEA was then purified and polymerized with methylene diphenyldiisocyanate and hexamethylene diisocyanate to produce polyureas PU-1 and PU-2 with excellent thermostability. This is the first study to synthesize polyureas from a microbial aromatic diamine, showing great potential for the industrial production of heat-resistant polymer materials.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)