Article
Reproductive Biology
Jun-Lin Song, Yu-Jiang Sun, Gui-Qin Liu, Guo-Liang Zhang
Summary: This study investigated the biological effects of DON and ZEA exposure on donkey endometrial epithelial cells (EECs). The results showed that DON exposure changed the expression levels of pyroptosis-associated genes, while ZEA exposure changed the expression levels of inflammation-associated genes. Furthermore, exposure to DON and ZEA affected the expression of cancer-promoting genes and genes related to androgen and estrogen secretion.
Article
Reproductive Biology
Kimberly M. Davenport, Eleanore ONeil, M. Sofia Ortega, Amanda Patterson, Andrew M. Kelleher, Wesley C. Warren, Thomas E. Spencer
Summary: This study utilized single-cell transcriptome analysis to identify different cell types and their ontogeny in bovine conceptus and chorion. The research revealed the role of key transcription factors in the development of trophoblasts and differentiation of binucleate cells. The digital atlas of cell types in the developing bovine conceptus serves as a valuable resource for understanding the genes and pathways involved in its development during critical periods.
BIOLOGY OF REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Subhashini Muhandiram, Keerthie Dissanayake, Toomos Orro, Kasun Godakumara, Suranga Kodithuwakku, Alireza Fazeli
Summary: Synchronized crosstalk between the embryo and endometrium during the periconception period is important for pregnancy establishment. The exchange of extracellular vesicles (EVs) of embryonic and endometrial origin plays a crucial role in embryo-maternal communication during peri-implantation. This study found that embryonic signals in the form of EVs can modulate the secretome of endometrial epithelial cells, enriching it with proteins that support embryo development, attachment, or implantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
GuangWei Zhou, PuXiu Shen, Yu Sun, Xing Zhang, ChenBo Yan, JingCheng Yu, Fang Liu, DeXin Yang, LiXin Deng, EnBu Xu, YiZhen Wang, Lin Liu, Chao Tong, Tao Sun, XueBing Wang
Summary: Endometritis is an inflammation that can lead to infertility in cows. This study used RNA sequencing to analyze gene expression changes in bovine endometrial epithelial cells induced by lipopolysaccharide (LPS)-induced inflammation. The results showed differential expression of 60 genes between LPS-treated and control cells, with enrichment in chemokine receptor binding, inflammatory response, and cytokine signaling pathways. The study provides insights into the molecular mechanism of endometritis and potential targets for therapeutic intervention.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Cell Biology
Daichi Nishino, Ai Kotake, Chi Sun Yun, Al-Nur Md Iftekhar Rahman, Mohamed El-Sharawy, Ken-ichi Yamanaka, M. A. M. Yahia Khandoker, Nobuhiko Yamauchi
Summary: Bovine endometrial epithelial cells can form gland-like structures and express specific genes when cultured in matrigel, and co-culture with stromal cells can increase the expression of certain genes. This highlights the potential use of matrigel for investigating endometrial function during the implantation period.
CELL AND TISSUE RESEARCH
(2021)
Article
Reproductive Biology
F. B. P. Wooding, A. J. Forhead, S. Wilsher, W. R. Allen, R. M. Roberts, J. A. Green, J. F. Beckers, N. Melo Sousa, G. Charpigny
Summary: Mature granulated trophoblast binucleate cells (BNC) are found in all ruminant placentas examined. However, the Giraffe shows a different protein expression pattern in BNC, with certain proteins only present in specific locations of the placental villi.
BIOLOGY OF REPRODUCTION
(2022)
Article
Cell Biology
Eliza Grbac, Teresa So, Swati Varshney, Nicholas Williamson, Evdokia Dimitriadis, Ellen Menkhorst
Summary: Research suggests that prednisolone affects the expression of cytokines and protein production in human endometrial stromal fibroblasts (hESF), altering their regulation of trophoblast cells. Decidualization of hESF in the presence of prednisolone enhances trophoblast cell growth and influences the expression of cell motility genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Hanako Bai, Manabu Kawahara, Masashi Takahashi
Summary: This study investigated the effect of vitamin K on bovine endometrial epithelial cells and found that menaquinone-4, the biologically active form of vitamin K, can regulate gene expression in these cells. The study identified 50 differentially expressed genes, some of which play important roles in reproductive events. Furthermore, menaquinone-4 was found to enhance cellular functions and modulate gene expression in bovine endometrial epithelial cells.
Article
Immunology
Kaiqiang Fu, Zezhi Wang, Rongfeng Cao
Summary: Berberine exerts anti-inflammatory effects by partially activating the Keap1/Nrf2 signaling pathway, which inhibits the inflammatory response induced by LPS in bEECs.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Reproductive Biology
Heather L. Chaney, Lindsay F. Grose, Gilles Charpigny, Susanta K. Behura, I. Martin Sheldon, James G. Cronin, Patrick Lonergan, Thomas E. Spencer, Daniel J. Mathew
Summary: This study identified biologically relevant IFNT-stimulated genes in specific endometrial cell types. The findings provide critical information on the effects of conceptus IFNT on specific endometrial compartments during early developmental processes in cattle.
BIOLOGY OF REPRODUCTION
(2021)
Article
Genetics & Heredity
Renwu Hua, Qiaorui Liu, Weisi Lian, Dengying Gao, Cheng Huang, Minggang Lei
Summary: This study reveals the regulatory mechanism of extracellular vesicles (EVs) in uterine fluids on endometrial epithelial cells (EECs) and embryonic trophoblast cells (PTr2 cells). Transcriptome sequencing analysis identifies a significant number of differentially expressed genes in both EECs and PTr2 cells after treatment. Real-time quantitative PCR (RT-qPCR) validation confirms the differential expression of these genes. Additionally, bioinformatics analysis reveals the functional involvement of these differentially expressed genes.
Review
Cell Biology
Fiona L. Cousins, Ronald Pandoy, Shiying Jin, Caroline E. Gargett
Summary: This review discusses the discovery of human and mouse endometrial epithelial stem/progenitor cells, their markers and location, as well as their roles in endometrial dynamics, including regeneration and re-epithelialization during the menstrual cycle. Additionally, it outlines the techniques used to identify these cells and their potential role in endometrial proliferative disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Mojtaba Najafi, Yongzhi Guo, Goran Andersson, Patrice Humblot, Erik Bongcam-Rudloff
Summary: LPS, a component of Gram-negative bacteria outer membrane, is involved in the pathogenic processes of mastitis and metritis in dairy cattle. In vitro studies have shown that LPS stimulates the proliferation of bovine endometrial epithelial cells. Transcriptomic analysis of these cells treated with LPS revealed differentially expressed genes associated with proliferation and apoptosis. The analysis also identified putative transcription factors and their interactions, providing insights into the mechanisms underlying the proliferative and apoptosis processes. Additionally, the study shed light on the early signaling and metabolic pathways involved in the immune response and cell proliferation in bovine endometrial epithelial cells infected with Escherichia coli-LPS.
Article
Agriculture, Dairy & Animal Science
M. A. Crookenden, A. V. R. Lake, C. R. Burke, J. T. Pratt, M. D. Mitchell, C. V. C. Phyn, J. R. Roche, A. Heiser
Summary: Chronic postpartum uterine infection has a negative impact on fertility. NSAIDs are commonly used to alleviate pain and treat inflammation in transition dairy cows. To study uterine inflammation without animal experiments, an in vitro model using cultured bovine endometrial epithelial cells was established. The effectiveness of 5 NSAIDs in dampening inflammation was measured by ELISA, and meloxicam, flunixin meglumine, and tolfenamic acid were found to be successful. Further gene expression analysis revealed downregulation of genes involved in chemokine signaling and the eicosanoid pathway. These findings suggest that these NSAIDs should be investigated for the treatment of postpartum uterine inflammation.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Biology
Paula C. C. Molinari, John J. Bromfield
Summary: Cattle under heat stress experience reduced fertility, milk production, and increased uterine infection. Exposure of endometrial cells to heat stress further increases expression of inflammatory mediators in response to bacterial components. Heat shock proteins HSP1A1 and HSF1 help to restrain inflammatory responses.
JOURNAL OF THERMAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Dongxia Hou, Min Su, Xiawei Li, Zhiying Li, Ting Yun, Yuhang Zhao, Ling Zhang, Lihua Zhao, Rongfeng Li, Haiquan Yu, Xueling Li
Article
Oncology
Ge Dong, Gui Ma, Rui Wu, Jinming Liu, Mingcheng Liu, Ang Gao, Xiawei Li, A. Jun, Xiaoyu Liu, Zhiqian Zhang, Baotong Zhang, Liya Fu, Jin-Tang Dong
Letter
Biochemistry & Molecular Biology
Baotong Zhang, Siyuan Xia, Mingcheng Liu, Xiawei Li, Shimin Shuai, Wei Tao, Yixiang Li, Jianping Jenny Ni, Wei Zhou, Lan Liao, Jianming Xu, Jin-Tang Dong
JOURNAL OF GENETICS AND GENOMICS
(2022)
Article
Oncology
Mingcheng Liu, Qingqing Huang, A. Jun, Linyue Li, Xiawei Li, Zhiqian Zhang, Jin-Tang Dong
Summary: Prostate cancer is a major cause of cancer-related deaths among men, requiring novel treatments. Deslanoside has shown anticancer activity in prostate cancer cells by modulating gene expression, inducing cell cycle arrest at G2/M, and promoting apoptosis. The altered expression of specific genes by deslanoside also correlates with survival outcomes in prostate cancer patients, suggesting potential for its repurposing as an anticancer drug.