Article
Genetics & Heredity
Carlo Crucianelli, Janhvi Jaiswal, Ananthakrishnan Vijayakumar Maya, Liyne Nogay, Andrea Cosolo, Isabelle Grass, Anne-Kathrin Classen
Summary: Regeneration relies on cell proliferation and multiple signaling pathways to restore damaged tissues. Different types of tissue damage activate distinct signaling pathways and accelerate the S-phase of the cell cycle to promote regenerative proliferation.
Article
Biochemistry & Molecular Biology
Dandan Zou, Wenlong Hu, Jie Qin, Zongfang Wei, Danyang Wang, Li Li
Summary: Neutrophils can take over the role of macrophages in the absence of the latter, actively participating in clearance after brain injury. Their migration to the wound during early stages of brain injury is regulated by MMP9 and MMP13.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Chemistry, Multidisciplinary
Ben Zhang, Yingchao Su, Juncen Zhou, Yufeng Zheng, Donghui Zhu
Summary: This review provides an in-depth analysis of immune response in tissue repair/regeneration and discusses various implant-mediated immunomodulation strategies. It focuses on major immune cells such as neutrophils, macrophages, and T cells, as well as methods to regulate immune response through physical, chemical, and biological cues. Finally, scaffold-facilitated regenerations of different tissue types are presented, highlighting the importance of immunomodulation in tissue regeneration.
Review
Immunology
Zewen Kelvin Tuong, Benjamin J. Stewart, Shuang Andrew Guo, Menna R. Clatworthy
Summary: Many immune cell subsets permanently reside in non-lymphoid organs, playing critical roles in tissue homeostasis and defense. Cell interactions and environmental cues shape immune cell function, and dysregulation can lead to inadequate immune responses. Understanding these mechanisms is crucial for studying tissue immunity.
IMMUNOLOGICAL REVIEWS
(2022)
Review
Oncology
Hillary G. Pratt, Kayla J. Steinberger, Nicole E. Mihalik, Sascha Ott, Thomas Whalley, Barbara Szomolay, Brian A. Boone, Timothy D. Eubank
Summary: The survival rates for patients with pancreatic adenocarcinoma are extremely low due to late detection and lack of effective treatments. Immunotherapy, a potential treatment method, has shown limited progress in pancreatic adenocarcinoma. This review explores the roles of macrophages and neutrophils as prominent immune cell types in the pancreatic tumor environment and how they impact the harsh tumor environment and standard of care therapies. Targeting macrophages and neutrophils could potentially improve outcomes for patients with pancreatic adenocarcinoma.
Article
Cell Biology
Shi-Peng Li, Xin-Qiang Li, Xiao-Jie Chen, Jin-Ming Zhang, Guang-Peng Zhou, Liu-Xin Zhou, Hai-Ming Zhang, Li-Ying Sun, Zhi-Jun Zhu
Summary: This study investigated the proinflammatory cytokines and differentially expressed proteins in a mouse model of liver transplantation using a protein chip system and proteomics. Bioinformatics analysis was employed to analyze the immune response activation-associated network. The results revealed significant changes in cytokine levels and identified key proteins associated with liver transplant rejection. These findings provide new insights and potential treatment targets for liver transplant rejection.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Immunology
Zhijun Pu, Xinyu Bao, Shengnan Xia, Pengfei Shao, Yun Xu
Summary: In this study, dauricine was found to reduce neuronal injury induced by ischemia-reperfusion by regulating the chemotaxis factor serpine1 in peripheral neutrophil infiltration.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Immunology
Zhenya Guo, Junze Chen, Yonglian Zeng, Zefeng Wang, Mei Yao, Stephen Tomlinson, Bin Chen, Guandou Yuan, Songqing He
Summary: In this study, the role of complement in cholestatic liver injury (CLI) was investigated. It was found that complement is involved in the infiltration and activation of neutrophils and macrophage M1 polarization in the liver. C3 deficiency and CR2-Crry significantly alleviated CLI. Inhibition of complement could preserve the protective function of macrophages in clearing lipopolysaccharide (LPS), suggesting that complement inhibition could be useful in treating CLI.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Xiaoling Qiang, Jianhua Li, Shu Zhu, Mingzhu He, Weiqiang Chen, Yousef Al-Abed, Max Brenner, Kevin J. Tracey, Ping Wang, Haichao Wang
Summary: The study suggests that DCD and its analog have protective effects in hepatic ischemia-reperfusion injury, possibly by attenuating inflammation through impairing the EGFR signaling pathway.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Stefan Wolf, Jenna H. Rannikko, Reetta Virtakoivu, Paolo Cinelli, Gunther Felmerer, Anna Burger, Pietro Giovanoli, Michael Detmar, Nicole Lindenblatt, Maija Hollmen, Epameinondas Gousopoulos
Summary: Lipedema is a chronic and progressive disorder characterized by the painful and disproportionate increase of subcutaneous fat in the extremities. This study reveals that CD163+ macrophages play a critical role in lipedema and re-polarization of these macrophages can normalize the accumulation of cellular lipids. These findings provide new insights into the pathophysiology of lipedema and suggest potential treatment options.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Ting Shang, Zhi-Sen Zhang, Xin-Tong Wang, Jing Chang, Meng-En Zhou, Ming Lyu, Shuang He, Jian Yang, Yan-Xu Chang, Yuefei Wang, Ming-Chun Li, Xiumei Gao, Yan Zhu, Yuxin Feng
Summary: The study demonstrates that XBJ can inhibit NETs formation by reversing the GSDMD-related pathway and protect against sepsis-induced lung injury.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Matthew D. Cheung, Elise N. Erman, Kyle H. Moore, Jeremie M. P. Lever, Zhang Li, Jennifer R. LaFontaine, Gelare Ghajar-Rahimi, Shanrun Liu, Zhengqin Yang, Rafay Karim, Bradley K. Yoder, Anupam Agarwal, James F. George
Summary: A study has found that there are 7 subpopulations of resident macrophages in the kidney, which have different functions and spatial distributions. Using single-cell RNA sequencing and spatial transcriptomics, researchers have localized and characterized these subpopulations, and discovered that their locations and transcriptomic signatures change following acute kidney injury, taking at least 28 days to fully restore.
Article
Biochemistry & Molecular Biology
Samu Himanen, Mikael C. Puustinen, Alejandro J. Da Silva, Anniina Vihervaara, Lea Sistonen
Summary: This study investigated how heat shock factors HSF1 and HSF2 coordinate transcription of genes and enhancers during oxidative stress and heat shock. The researchers found that the release of paused RNA polymerase II is a common mechanism regulating gene transcription, while enhancers are activated through the recruitment of Pol II. HSF1 and HSF2 also function as stress-responsive factors that activate specific genes and enhancers in a stress type-specific manner. HSF1 activates chaperone genes specifically in heat-shocked cells, while it trans-activates oxidative stress-specific genes upon oxidative stress.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Engineering, Environmental
Weiwen Yin, Baogang Zhang, Han Zhang, Daxin Zhang, Tiina Leiviska
Summary: This study investigated the distribution of vanadium (V) and microplastics in soil at a V smelting site and their effects on microbial community dynamics and assembly. The results showed that both V and microplastics were present in the soil profile, with different microbial community compositions in the topsoil and subsoil. V and microplastics had direct impacts on the microbial structure in the topsoil and indirect influences in the subsoil. Deterministic processes were more prevalent in community assembly in the topsoil, while stochastic processes governed the subsoil. The interspecific relationship was closer in the topsoil with greater network complexity and higher modularity.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Cell Biology
Eileen Uribe-Querol, Carlos Rosales
Summary: Obesity is characterized by increased body weight and exaggerated enlargement of adipose tissue. It is associated with various pathological complications and systemic inflammation caused by adipocyte stress, infiltration of neutrophils and other immune cells.
Article
Neurosciences
Ildiko Kasza, Jens-Peter Kuehn, Henry Voelzke, Diego Hernando, Yaohui G. Xu, John W. Siebert, Angela L. F. Gibson, C. -L. Eric Yen, David W. Nelson, Ormond A. MacDougald, Nicole E. Richardson, Dudley W. Lamming, Philip A. Kern, C. M. Alexander
Summary: This study looks at the distinct strategies used by mammals to maintain body temperature, focusing on the role of skin-associated adipose tissues in heat production and conservation. The research found that different adipose depots in mice and humans play varying roles in regulating heat production, with implications for thermal defense. Furthermore, the study highlights the unique regulation of dermal white adipose tissue in mice and the direct heat-producing ability of human skin-associated fat depots as alternatives to brown adipose tissues.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Surgery
David G. Greenhalgh, Robert Cartotto, Sandra L. Taylor, Jeffrey R. Fine, Giavonni M. Lewis, David J. Smith, Michael A. Marano, Angela Gibson, Lucy A. Wibbenmeyer, James H. Holmes, Julie A. Rizzo, Kevin N. Foster, Anjay Khandelwal, Sarah Fischer, Mark R. Hemmila, David Hill, Ariel M. Aballay, Edward E. Tredget, Jeremy Goverman, Herbert Phelan, Carlos J. Jimenez, Anthony Baldea, Rajiv Sood
Summary: ABRUPT study aimed to investigate the resuscitation practices of burn patients with albumin or crystalloids and design a future trial. The study found that albumin use was associated with older age, larger and deeper burns, and more severe organ dysfunction at presentation.
Article
Immunology
Emily F. Eix, Chad J. Johnson, Kayla M. Wartman, John F. Kernien, Jennifer J. Meudt, Dhanansayan Shanmuganayagam, Angela L. F. Gibson, Jeniel E. Nett
Summary: Candida auris can efficiently colonize the skin of patients, leading to nosocomial outbreaks. However, other Candida species, including those closely related to C. auris, do not show the same high growth in the skin microenvironment. This study provides two ex vivo skin models for C. auris colonization, which can be used to compare clinical isolates and genetically mutated strains.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Virology
Edward L. Evans, Ginger M. Pocock, Gabriel Einsdorf, Ryan T. Behrens, Ellen T. A. Dobson, Marcel Wiedenmann, Christian Birkhold, Paul Ahlquist, Kevin W. Eliceiri, Nathan M. Sherer
Summary: This article introduces an automated imaging strategy, HIV RGB, for studying single-cell RNA regulation of HIV-1. Different fluorescently tagged viral RNA and protein species are recorded using multicolor long-term time-lapse video microscopy, and image processing is performed using an open-source workflow called NR-SAT based on ImageJ plugins. This method can be adapted to study various dynamic viral or cellular processes.
Article
Critical Care Medicine
James H. Holmes, Leopoldo C. Cancio, Jeffrey E. Carter, Lee D. Faucher, Kevin Foster, Helen D. Hahn, Booker T. King, Randi Rutan, Janice M. Smiell, Richard Wu, Angela L. F. Gibson
Summary: This analysis examines the safety data of two clinical trials evaluating the safety and efficacy of StrataGraft in patients with deep partial-thickness burns. The results show that StrataGraft is well-tolerated by patients and offers a safe alternative to autograft for this type of burn.
Article
Cell Biology
J. Z. Alex Cheong, Aiping Liu, Clayton J. Rust, Collin L. Tran, Sameeha E. Hassan, Lindsay R. Kalan, Angela L. F. Gibson
Summary: This study evaluated the cytotoxicity and antimicrobial activity of chlorhexidine gluconate (CHG), a wound cleansing agent, in human skin using clinically relevant models. The results showed that CHG significantly affected the metabolic activity of skin explants and had persistent cytotoxicity, impairing wound healing in vivo. CHG also had limited antimicrobial activity, with viable bacterial cells residing deep within wound tissue even after CHG application.
WOUND REPAIR AND REGENERATION
(2022)
Article
Surgery
Jocelyn C. Zajac, Aiping Liu, Adam J. Uselmann, Christie Lin, Sameeha E. Hassan, Lee D. Faucher, Angela L. F. Gibson
Summary: The study demonstrated the potential of SWIG in identifying burn necrosis and determining the interface between viable and nonviable tissue, while highlighting the inconsistencies and lack of standardization in ICGA for evaluating burn injuries.
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS
(2022)
Letter
Biochemical Research Methods
Curtis T. Rueden, Mark C. Hiner, Edward L. Evans, Michael A. Pinkert, Alice M. Lucas, Anne E. Carpenter, Beth A. Cimini, Kevin W. Eliceiri
Article
Biology
Elizabeth M. Haynes, Korri H. Burnett, Jiaye He, Marcel W. Jean-Pierre, Martin Jarzyna, Kevin W. Eliceiri, Jan Huisken, Mary C. Halloran
Summary: This study reveals that KLC4, a subunit of the motor protein kinesin-1, plays a crucial role in regulating axon branching and arborization pattern of sensory neurons during development. KLC4 is required for stabilization of nascent axon branches, proper microtubule dynamics, and endosomal transport. Additionally, KLC4 is involved in establishing molecular differences between central and peripheral axons, and is implicated in stress response circuits.
Article
Chemistry, Multidisciplinary
Samir Rosas, Keegan A. Schoeller, Edward Chang, Hongyan Mei, Mikhail A. Kats, Kevin W. Eliceiri, Xinyu Zhao, Filiz Yesilkoy
Summary: Label-free and nondestructive mid-infrared vibrational hyperspectral imaging is an important tissue analysis tool that provides spatially resolved biochemical information. This study introduces an advanced mid-infrared spectrochemical tissue imaging modality using metasurfaces to capture quantitative molecular maps of large-area murine brain tissue sections. The results demonstrate that plasmonic metasurfaces enhance chemical contrast in infrared images and enable the detection of ultrathin tissue regions. This imaging method has the potential to have a broad impact on translational research and clinical histopathology.
ADVANCED MATERIALS
(2023)
Article
Biology
Han Nim Lee, Jenu Varghese Chacko, Ariadna Gonzalez Solis, Kuo-En Chen, Jessica A. S. Barros, Santiago Signorelli, A. Harvey Millar, Richard David Vierstra, Kevin W. Eliceiri, Marisa S. Otegui, Yoselin Benitez-Alfonso
Summary: This study reveals a new function of NBR1 in the degradation of damaged chloroplasts through microautophagy, where NBR1 associates with photodamaged chloroplasts independently of ATG7 and delivers them to vacuoles for degradation.
Editorial Material
Biochemical Research Methods
Beth A. Cimini, Kevin W. Eliceiri
Letter
Biochemical Research Methods
Gabriel J. Selzer, Curtis T. Rueden, Mark C. Hiner, Edward L. Evans, Kyle I. S. Harrington, Kevin W. Eliceiri
Editorial Material
Biochemical Research Methods
Anne E. Carpenter, Beth A. Cimini, Kevin W. Eliceiri
Article
Biochemical Research Methods
Zheyu Li, Bin Li, Kevi W. Eliceiri, Vijaykrishnan Narayanan
Summary: This paper presents a computation efficient analysis workflow for WSIs classification based on compression domain processing. By leveraging the pyramidal magnification structure of WSI files and compression domain features, the methods assign different decompression depths to the patches of WSIs, reducing unnecessary access to high zoom levels and expensive full decompression.
BIOMEDICAL OPTICS EXPRESS
(2023)