Article
Oncology
Changshu Li, Jun Shao, Peiyi Li, Jiaming Feng, Jingwei Li, Chengdi Wang
Summary: Despite advancements in diagnosis and treatment, lung cancer still has a high morbidity and mortality rate worldwide. Tissue biopsy, the current gold standard for diagnosis, has limitations. Liquid biopsy, particularly the use of circulating tumor DNA, has emerged as a promising tool for early diagnosis and monitoring of lung cancer. This review explores the biological characteristics of ctDNA, detection methods, and its role in lung cancer management.
Review
Oncology
Gabriella Cirmena, Martina Dameri, Francesco Ravera, Piero Fregatti, Alberto Ballestrero, Gabriele Zoppoli
Summary: Liquid biopsy, which aims to identify tumor biomarkers through noninvasive analysis of body fluids, is a promising approach for cancer screening, but its clinical applications are currently limited due to the lack of standardized pre-analytical procedures and technical challenges.
Article
Medicine, General & Internal
Aitor Rodriguez-Casanova, Aida Bao-Caamano, Ramon M. Lago-Leston, Elena Brozos-Vazquez, Nicolas Costa-Fraga, Isabel Ferreiros-Vidal, Ihab Abdulkader, Yolanda Vidal-Insua, Francisca Vazquez Rivera, Sonia Candamio Folgar, Rafael Lopez-Lopez, Laura Muinelo-Romay, Angel Diaz-Lagares
Summary: The study demonstrates that targeted NGS analysis of cfDNA with TST170 can effectively detect gene variants in metastatic CRC patients, showing high concordance with tumor tissue and BEAMing analysis.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Jozsef Petrik, Donatella Verbanac, Marija Fabijanec, Andrea Hulina-Tomaskovic, Andrea Ceri, Anita Somborac-Bacura, Roberta Petlevski, Marija Grdic Rajkovic, Lada Rumora, Bozo Kruslin, Mario Stefanovic, Neven Ljubicic, Neven Barsic, Antonija Hanzek, Luka Bockor, Ivana Celap, Alma Demirovic, Karmela Barisic
Summary: This review provides an overview of the research conducted on circulating tumor cells (CTCs) with the aim of finding suitable detection systems and assessing the possibility of clinical applications in patients with colorectal cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Yanbing Lu, Xin Li, Yun Liu, Jiabin Li, Zhezhou Chen, Xianmeng Meng, Wanming Li, Jin Fang
Summary: Liquid biopsy, using a W3-based molecular beacon (MAB-W3-3G), can detect CTCs and exosomes in the blood of tumor patients. This method has high specificity, plasma stability, and can be completed without sample pretreatment or routine washing procedures. The enumeration of W3-positive CTCs and quantitation of positive exosomes showed potential for more precise clinical diagnosis of colorectal cancer (CRC).
ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Lavanya Sivapalan, Graeme J. Thorn, Emanuela Gadaleta, Hemant M. Kocher, Helen Ross-Adams, Claude Chelala
Summary: This study explores the utility of circulating tumour DNA (ctDNA) in longitudinal tumour monitoring of pancreatic ductal adenocarcinoma (PDAC). The results show that ctDNA is sensitive in monitoring disease burden and can be used to track clonal evolution and evaluate treatment response.
Article
Biotechnology & Applied Microbiology
Joao M. Alves, Nuria Estevez-Gomez, Monica Valecha, Sonia Prado-Lopez, Laura Tomas, Pilar Alvarino, Roberto Pineiro, Laura Muinelo-Romay, Patricia Mondelo-Macia, Mercedes Salgado, Agueda Iglesias-Gomez, Laura Codesido-Prada, Joaquin Cubiella, David Posada
Summary: The study compared the genomic profiles of CTC pools from mCRC patients using different enrichment strategies and found that Parsortix and some CellSearch CTC pools closely matched the genomic features of primary tumor samples, while FACS CTC pools contained more sequencing artifacts.
Article
Cell Biology
Valentina Magri, Luca Marino, Chiara Nicolazzo, Angela Gradilone, Gianluigi De Renzi, Michela De Meo, Orietta Gandini, Arianna Sabatini, Daniele Santini, Enrico Cortesi, Paola Gazzaniga
Summary: This study aimed to evaluate the clinical value of circulating tumor cell (CTC) dynamics in metastatic colorectal cancer (mCRC) patients receiving first-line treatments. The CTC data from 218 patients were analyzed and four prognostic trajectories were identified. The study confirmed the clinical significance of CTC positivity and suggested that CTC trajectories are better prognostic indicators than CTC enumeration at baseline, providing potential biomarkers to monitor first-line treatments.
Review
Oncology
Mohammad Amin Kerachian, Marjan Azghandi, Sina Mozaffari-Jovin, Alain R. Thierry
Summary: Methylation analysis of circulating cell-free DNA (cirDNA) has significant potential in advancing cancer detection, prognosis, and treatment, as well as genetic disorders. The choice of pre-analytical variables can impact cirDNA methylation identification, while different techniques yield varying results. Despite efforts to simplify methylation analysis with new technologies, the process remains complex and lacks a standard method for pre-analytical processing of methylated cirDNA.
CLINICAL EPIGENETICS
(2021)
Review
Oncology
Isabel Heidrich, Thaer S. A. Abdalla, Matthias Reeh, Klaus Pantel
Summary: Colorectal cancer is a common malignancy with high mortality rates, often due to tumor cells spreading through the blood. Liquid biopsy, which analyzes circulating tumor cells and cell-free DNA in the blood, offers new diagnostic and therapeutic opportunities for colorectal cancer patients. It has the potential to improve early detection, monitor disease progression, and guide personalized treatment strategies.
Review
Pharmacology & Pharmacy
Xiaoshuai Li, Qiushi Wang, Rui Wang
Summary: Exosomes are extracellular vesicles that mediate cell-to-cell communication and contain bioactive substances such as DNA, RNA, lipids, and proteins. The DNA in exosomes, mainly consisting of genomic DNA and mitochondrial DNA, plays a crucial role in liquid biopsy of colorectal cancer and immune metabolic regulation. However, research on exosome DNA is still limited.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Effat Alemzadeh, Leila Allahqoli, Hamideh Dehghan, Afrooz Mazidimoradi, Alireza Ghasempour, Hamid Salehiniya
Summary: Liquid biopsy, which includes circulating tumor cells and circulating tumor DNA, is increasingly popular in diagnosing and managing breast cancer. These biomarkers can be used as markers for cancer progression and understanding metastasis and treatment resistance, providing a non-invasive method for cancer assessment and monitoring. However, more studies are needed to confirm its clinical usage in cancer diagnosis among patients. This review focuses on the application of circulating tumor biomarkers, particularly in breast cancer.
Article
Oncology
Ami N. Shah, Kristen J. Carroll, Lorenzo Gerratana, Chenyu Lin, Andrew A. Davis, Qiang Zhang, Saya Jacob, Brian Finkelman, Youbin Zhang, Wenen Qiang, Paolo D'Amico, Carolina Reduzzi, William J. Gradishar, Amir Behdad, Massimo Cristofanilli
Summary: Data shows that premenopausal women diagnosed with metastatic breast cancer have unique clinical, pathological, and molecular features compared to their postmenopausal counterparts, potentially impacting treatment options.
BREAST CANCER RESEARCH AND TREATMENT
(2021)
Article
Oncology
Sungun Bang, Dongju Won, Saeam Shin, Kang Su Cho, Jae Won Park, Jongsoo Lee, Young Deuk Choi, Suwan Kang, Seung-Tae Lee, Jong Rak Choi, Hyunho Han
Summary: In our study, we discovered genetic mutations in the DNA of cancer cells obtained from the blood of metastatic prostate cancer patients. These mutations were more common in patients with aggressive and progressing cancer that did not respond to conventional treatment. Compared to the traditional method of measuring prostate-specific antigen (PSA) levels, these genetic mutations were more sensitive in detecting prostate cancer progression. This suggests that a simple blood test could be used to track genetic mutations, identify aggressive cancer subtypes, monitor disease progression, and improve the management of advanced prostate cancer.
Review
Endocrinology & Metabolism
M. T. Miele, E. Qorri, X. Borshi, P. Avantaggiato, V Candotto
Summary: Early shedding of tumor cells in primary tumors and their circulation in the bloodstream can be analyzed to improve cancer treatment stratification and understand the impact of treatment on cancer cell selection.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2022)
Review
Surgery
Alejandro Marin-Buck, Erbil Karaman, Juan J. Amer-Cuenca, Juan F. Lison, Peter Torok, Onur Karaaslan, Gaetano Valenti, Gabriella Zito, Antonio Biondi, Benito Chiofalo
Summary: Uterine fibroids are common benign tumors in the female genital tract, often managed through minimally invasive surgical techniques. Myomectomy is the standard surgical treatment for preserving fertility, and various minimally invasive options can be chosen based on the characteristics of the fibroids and the surgeon's skill level. A less invasive surgical approach known as mini-laparotomy has also been proposed as an alternative to established minimally invasive options. This review aims to discuss recent advances in minimally invasive techniques for myomectomy and compare them with the mini-laparotomy approach.
JOURNAL OF INVESTIGATIVE SURGERY
(2021)
Review
Medicine, General & Internal
Antonio Maria Borzi, Antonio Biondi, Francesco Basile, Marco Vacante
WIENER KLINISCHE WOCHENSCHRIFT
(2020)
Article
Surgery
Yara Abdel Khalek, Roger Bitar, Costas Christoforou, Simone Garzon, Alessandro Tropea, Antonio Biondi, Zaki Sleiman
UPDATES IN SURGERY
(2020)
Article
Gastroenterology & Hepatology
Sudabeh Alatab, Sadaf G. Sepanlou, Kevin Ikuta, Homayoon Vahedi, Catherine Bisignano, Saeid Safiri, Anahita Sadeghi, Molly R. Nixon, Amir Abdoli, Hassan Abolhassani, Vahid Alipour, Majid A. H. Almadi, Amir Almasi-Hashiani, Amir Anushiravani, Jalal Arabloo, Suleman Atique, Ashish Awasthi, Alaa Badawi, Atif A. A. Baig, Neeraj Bhala, Ali Bijani, Antonio Biondi, Antonio M. Borzi, Kristin E. Burke, Felix Carvalho, Ahmad Daryani, Manisha Dubey, Aziz Eftekhari, Eduarda Fernandes, Joao C. Fernandes, Florian Fischer, Arvin Haj-Mirzaian, Arya Haj-Mirzaian, Amir Hasanzadeh, Maryam Hashemian, Simon Hay, Chi L. Hoang, Mowafa Househ, Olayinka S. Ilesanmi, Nader Jafari Balalami, Spencer L. James, Andre P. Kengne, Masoud M. Malekzadeh, Shahin Merat, Tuomo J. Meretoja, Tomislav Mestrovic, Erkin M. Mirrakhimov, Hamed Mirzaei, Karzan A. Mohammad, Ali H. Mokdad, Lorenzo Monasta, Ionut Negoi, Trang H. Nguyen, Cuong T. Nguyen, Akram Pourshams, Hossein Poustchi, Mohammad Rabiee, Navid Rabiee, Kiana Ramezanzadeh, David L. Rawaf, Salman Rawaf, Nima Rezaei, Stephen R. Robinson, Luca Ronfani, Sonia Saxena, Masood Sepehrimanesh, Masood A. Shaikh, Zeinab Sharafi, Mehdi Sharif, Soraya Siabani, Ali Reza Sima, Jasvinder A. Singh, Amin Soheili, Rasoul Sotoudehmanesh, Hafiz Ansar Rasul Suleria, Berhe E. Tesfay, Bach Tran, Derrick Tsoi, Marco Vacante, Adam B. Wondmieneh, Afshin Zarghi, Zhi-Jiang Zhang, Mae Dirac, Reza Malekzadeh, Mohsen Naghavi
LANCET GASTROENTEROLOGY & HEPATOLOGY
(2020)
Article
Gastroenterology & Hepatology
Sadaf G. Sepanlou, Saeid Safiri, Catherine Bisignano, Kevin S. Ikuta, Shahin Merat, Mehdi Saberifiroozi, Hossein Poustchi, Derrick Tsoi, Danny V. Colombara, Amir Abdoli, Rufus Adesoji Adedoyin, Mohsen Afarideh, Sutapa Agrawal, Sohail Ahmad, Elham Ahmadian, Ehsan Ahmadpour, Tomi Akinyemiju, Chisom Joyqueenet Akunna, Vahid Alipour, Amir Almasi-Hashiani, Abdulaziz M. Almulhim, Rajaa M. Al-Raddadi, Nelson Alvis-Guzman, Nahla Hamed Anber, Colin Angus, Amir Anoushiravani, Jalal Arabloo, Ephrem Mebrahtu Araya, Daniel Asmelash, Bahar Ataeinia, Zerihun Ataro, Maha Moh'd Wahbi Atout, Floriane Ausloos, Ashish Awasthi, Alaa Badawi, Maciej Banach, Diana Fernanda Bejarano Ramirez, Akshaya Srikanth Bhagavathula, Neeraj Bhala, Krittika Bhattacharyya, Antonio Biondi, Srinivasa Rao Bolla, Archith Boloor, Antonio M. Borzi, Zahid A. Butt, Luis L. A. Alberto Camera, Ismael R. Campos-Nonato, Felix Carvalho, Dinh-Toi Chu, Sheng-Chia Chung, Paolo Angelo Cortesi, Vera M. Costa, Benjamin C. Cowie, Ahmad Daryani, Barbora de Courten, Gebre Teklemariam Demoz, Rupak Desai, Samath Dhamminda Dharmaratne, Shirin Djalalinia, Hoa Thi Do, Fariba Dorostkar, Thomas M. Drake, Manisha Dubey, Bruce B. Duncan, Andem Effiong, Aziz Eftekhari, Aisha Elsharkawy, Arash Etemadi, Mohammad Farahmand, Farshad Farzadfar, Eduarda Fernandes, Irina Filip, Florian Fischer, Ketema Bizuwork Bizuwork Gebremedhin, Birhanu Geta, Syed Amir Gilani, Paramjit Singh Gill, Reyna Alma Gutierrez, Michael Tamene Haile, Arvin Haj-Mirzaian, Saeed S. Hamid, Milad Hasankhani, Amir Hasanzadeh, Maryam Hashemian, Hamid Yimam Hassen, Simon I. Hay, Khezar Hayat, Behnam Heidari, Andualem Henok, Chi Linh Hoang, Mihaela Hostiuc, Sorin Hostiuc, Vivian Chia-rong Hsieh, Ehimario U. Igumbor, Olayinka Stephen Ilesanmi, Seyed Sina Naghibi Irvani, Nader Jafari Balalami, Spencer L. James, Panniyammakal Jeemon, Ravi Prakash Jha, Jost B. Jonas, Jacek Jerzy Jozwiak, Ali Kabir, Amir Kasaeian, Hagazi Gebremedhin Kassaye, Adane Teshome Kefale, Rovshan Khalilov Muhammad Ali Khan, Ejaz Ahmad Khan, Amir Khater, Yun Jin Kim, Ai Koyanagi, Carlo La Vecchia, Lee-Ling Lim, Alan D. Lopez, Stefan Lorkowski, Paulo A. Lotufo, Rafael Lozano, Muhammed Magdy Abd El Razek, Hue Thi Mai, Navid Manafi, Amir Manafi, Mohammad Ali Mansournia, Lorenzo Giovanni Mantovani, Giampiero Mazzaglia, Dhruv Mehta, Walter Mendoza, Ritesh G. Menezes, Melkamu Merid Mengesha, Tuomo J. Meretoja, Tomislav Mestrovic, Bartosz Miazgowski, Ted R. Miller, Erkin M. Mirrakhimov, Prasanna Mithra, Babak Moazen, Masoud Moghadaszadeh, Abdollah Mohammadian-Hafshejani, Shafiu Mohammed, Ali H. Mokdad, Pablo A. Montero-Zamora, Ghobad Moradi, Mukhammad David Naimzada, Vinod Nayak, Ionut Negoi, Trang Huyen Nguyen, Richard Ofori-Asenso, In-Hwan Oh, Tinuke O. Olagunju, Jagadish Rao Padubidri, Keyvan Pakshir, Adrian Pana, Mona Pathak, Akram Pourshams, Navid Rabiee, Amir Radfar, Alireza Rafiei, Kiana Ramezanzadeh, Saleem Muhammad M. Rana, Salman Rawaf, David Laith Rawaf, Robert C. Reiner, Leonardo Roever, Robin Room, Gholamreza Roshandel, Saeed Safari, Abdallah M. Samy, Juan Sanabria, Benn Sartorius, Maria Ines Schmidt, Subramanian Senthilkumaran, Masood Ali Shaikh, Mehdi Sharif, Amrollah Sharifi, Mika Shigematsu, Jasvinder A. Singh, Amin Soheili, Hafiz Ansar Rasul Suleria, Berhane Fseha Teklehaimanot, Berhe Etsay Tesfay, Marco Vacante, Amir Vahedian-Azimi, Pascual R. Valdez, Tommi Juhani Vasankari, Giang Thu Vu, Yasir Waheed, Kidu Gidey Weldegwergs, Andrea Werdecker, Ronny Westerman, Dawit Zewdu Wondafrash, Adam Belay Wondmieneh, Yordanos Gizachew Yeshitila, Naohiro Yonemoto, Chuanhua Yu, Zoubida Zaidi, Afshin Zarghi, Shira Zelber-Sagi, Kaleab Alemayehu Zewdie, Zhi-Jiang Zhang, Xiu-Ju Zhao, Mohsen Naghavi, Reza Malekzadeh
LANCET GASTROENTEROLOGY & HEPATOLOGY
(2020)
Article
Surgery
Giovanni Scavone, Daniele Carmelo Caltabiano, Fabrizio Gulino, Maria Vittoria Raciti, Amy Giarrizzo, Antonio Biondi, Luigi Piazza, Antonio Scavone
UPDATES IN SURGERY
(2020)
Review
Environmental Sciences
Pietro Crispino, Miriam Gino, Elena Barbagelata, Tiziana Ciarambino, Cecilia Politi, Immacolata Ambrosino, Rosalia Ragusa, Marina Marranzano, Antonio Biondi, Marco Vacante
Summary: Parkinson's disease significantly impacts health-related quality of life, with gender differences observed in various aspects. Women with Parkinson's disease tend to have better outcomes in emotion processing and cognitive functions, while men fare better in the cognitive domain of health-related quality of life. Studies also note differences in the association between health-related quality of life and non-motor symptoms, with depression and fatigue particularly affecting women.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Review
Obstetrics & Gynecology
Zaki Sleiman, Roger Bitar, Costas Christoforou, Peter Torok, Michele Fichera, Marco Marzio Panella, Antonio Biondi, Antonio Simone Lagana, Simone Garzon, Ghida Radwan
Summary: This systematic review examined ten studies on the translation of laparoscopic psychomotor skills acquired in dry lab training into clinical practice, showing improved performance in intervention groups with fewer errors. However, no clinical patient outcomes were addressed in these studies, and all trials were at high risk of bias. Further studies with better designs are needed for more robust evidence.
JOURNAL OF OBSTETRICS AND GYNAECOLOGY
(2022)
Article
Medicine, General & Internal
Antonio Biondi, Gianluca Di Mauro, Riccardo Morici, Giuseppe Sangiorgio, Marco Vacante, Francesco Basile
Summary: This observational study compared the outcomes of intracorporeal anastomosis (ICA) and extracorporeal anastomosis (ECA) in patients undergoing laparoscopic right hemicolectomy for right colon cancer (RCC). The study found that ICA resulted in significantly faster bowel function recovery and shorter length of hospital stay compared to ECA, with no significant differences in postoperative complications between the two groups.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Infectious Diseases
Giuseppe Sangiorgio, Marco Vacante, Francesco Basile, Antonio Biondi
Summary: This study aims to assess the efficacy of parenteral and oral antibiotic prophylaxis compared to parenteral-only prophylaxis for preventing surgical site infection in laparoscopic surgery for colorectal cancer resection. The results showed that a combination of oral and intravenous antibiotics significantly reduces the incidence of surgical site infection in comparison to intravenous antibiotics alone.
Review
Biochemistry & Molecular Biology
Marco Vacante, Roberto Ciuni, Francesco Basile, Antonio Biondi
