Article
Oncology
Qianyu Lin, Yaqi Zhou, Jie Ma, Sanyang Han, Yunchuanxiang Huang, Feng Wu, Xuejuan Wang, Yanan Zhang, Xueshuang Mei, Lan Ma
Summary: In this study, the limitations of single-cell transcriptomics in capturing epithelial/tumour cells in nasopharyngeal carcinoma (NPC) were addressed by analyzing the transcriptomics and spatial characteristics at single-cell resolution. The findings reveal multiple patterns of immune escape mechanisms in NPC, including the loss of MHC molecules, induction of epithelial-mesenchymal transition, and the use of hyperplastic cells. Additionally, a specific CD8+ natural killer cell cluster in the NPC TME was identified for the first time. These insights into NPC immune landscape could lead to novel therapeutic strategies.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Oncology
Wen-Sa Peng, Xin Zhou, Wen-Bin Yan, Yu-Jiao Li, Cheng-Run Du, Xiao-Shen Wang, Chun-Ying Shen, Qi-Feng Wang, Hong-Mei Ying, Xue-Guan Lu, Ting-Ting Xu, Chao-Su Hu
Summary: This study investigates the heterogeneity of immune cells and tumor cells in recurrent nasopharyngeal carcinoma (rNPC) using single-cell profiling. The findings reveal increased infiltration of regulatory T cells and dysfunctional CD8(+) T cells in rNPC, as well as enhanced immune-related features in malignant cells. The study provides important insights for the development of targeted immunotherapy for rNPC.
Article
Cell Biology
Xiaojie Shi, Junyan Pan, Fufang Qiu, Liqin Wu, Xuyan Zhang, Yan Feng, Xiaoyi Gu, Jikuang Zhao, Wenwei Zheng
Summary: This study developed a novel computational framework to analyze the transcriptome of nasopharyngeal carcinoma (NPC) at multiple scales. The analysis revealed functional enrichments related to cell cycle acceleration, epithelial-mesenchymal transition, and chemokine-modulated inflammatory response during NPC progression. In addition, a chemokine called CXCL10 was found to recruit cytotoxic T cells in the NPC microenvironment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Xishan Chen, Renba Liang, Huan Lin, Kaihua Chen, Li Chen, Ge Tian, Xiaodong Zhu
Summary: Downregulation of CD166 inhibited CSC-related genes and decreased pEGFR and pERK expression in vitro and in vivo. The sphere formation and tumorigenesis ability of CD166-shRNA cells were significantly reduced. Additionally, EGF-stimulated CD166-shRNA cells showed an increase in CSC-like traits by activating the EGFR/ERK1/2 signaling pathway.
Article
Oncology
Shuzhen Guan, Lin Feng, Jinrui Wei, Guizhen Wang, Lichuan Wu
Summary: This study reveals that replication factor c subunit 4 (RFC4) plays a key role in the development of nasopharyngeal carcinoma (NPC), and its overexpression is associated with NPC cell proliferation. HOXA10 is confirmed as a downstream target of RFC4, and the overexpression of HOXA10 can attenuate RFC4 silencing-induced cell proliferation inhibition and cell cycle arrest. This study provides novel insights into the role of RFC4 in NPC cell proliferation and its pivotal role in NPC tumorigenesis.
FRONTIERS OF MEDICINE
(2023)
Article
Neurosciences
Thomas Baumgartner, Jan Hausfeld, Miguel dos Santos, Daria Knoch
Summary: Research indicates that independent punishers have larger gray matter volume in the right temporo-parietal junction associated with social cognition, while conditional punishers show larger gray matter volume in the right dorsolateral prefrontal cortex linked to behavioral control and strategic reasoning. Both independent punishers and nonpunishers exhibit larger gray matter volume in an area involved in the processing of social and monetary rewards, the bilateral caudate. Using a neural trait approach, these three types were clearly differentiated based on their neural signatures, shedding light on underlying psychological mechanisms.
HUMAN BRAIN MAPPING
(2021)
Article
Multidisciplinary Sciences
Yang Liu, Shuai He, Xi-Liang Wang, Wan Peng, Qiu-Yan Chen, Dong-Mei Chi, Jie-Rong Chen, Bo-Wei Han, Guo-Wang Lin, Yi-Qi Li, Qian-Yu Wang, Rou-Jun Peng, Pan-Pan Wei, Xiang Guo, Bo Li, Xiaojun Xia, Hai-Qiang Mai, Xue-Da Hu, Zemin Zhang, Yi-Xin Zeng, Jin-Xin Bei
Summary: Nasopharyngeal carcinoma is characterized by a heterogeneous microenvironment, with single cell sequencing revealing different cell types including immune-suppressive T regulatory, tolerogenic dendritic, and exhausted CD8 T cells. Intensive inter-cell interactions are observed, potentially promoting the formation of an immune-suppressive niche in the TME.
NATURE COMMUNICATIONS
(2021)
Editorial Material
Hematology
Pedro Farinha
Summary: In this study, the researchers identified MYCN as a novel oncogenic driver in mature T-cell lymphoma using a well-designed mouse model. MYCN directly cooperates with enhancer of zeste homolog 2 (EZH2) and may represent a targetable mechanism implicated in the pathogenesis of T-cell lymphoma.
Article
Biochemistry & Molecular Biology
Shi-Wei He, Yuan Zhang, Lei Chen, Wei-Jie Luo, Xiao-Min Li, Yang Chen, Sheng-Yan Huang, Qing-Mei He, Xiao-Jing Yang, Ying-Qin Li, Na Liu, Yin Zhao, Jun Ma
Summary: The combination of gemcitabine and cisplatin significantly inhibits NPC cell proliferation and tumor growth by inducing cell cycle arrest, cell apoptosis, and DNA damage response, revealing the mechanisms of improving locoregionally advanced NPC with combined GEM and DDP induction chemotherapy.
Article
Pharmacology & Pharmacy
Shiya Liu, Zhiyuan Wang, Daoqi Zhu, Jiabin Yang, Dandan Lou, Ruijiao Gao, Zetai Wang, Aiwu Li, Ying Lv, Qin Fan
Summary: Shengmai Yin has been shown to modulate DNA methylation levels and gene expression of nasopharyngeal carcinoma cells, enhancing sensitivity to radiotherapy and inhibiting resistance, migration, and invasion properties.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2021)
Article
Computer Science, Information Systems
Muting Wang, Stella W. Pang
Summary: This study developed a two-layer scaffold platform to mimic the extracellular matrix of the tissue for investigating the migration behaviors of nasopharyngeal carcinoma and epithelial cells. Results showed that NPC43 and NP460 cells responded differently to the topographic cues, with a high separation efficiency achieved under certain conditions.
Article
Oncology
Ying Fu, Xiuzhi Zhang, Xujun Liang, Yongheng Chen, Zhuchu Chen, Zhefeng Xiao
Summary: This study found that CapG is up-regulated in nasopharyngeal carcinoma tissues and cells. CapG promotes migration and invasion of nasopharyngeal carcinoma cells, possibly through the phosphorylation of MLC2. In contrast, Rac1 activation has no significant impact on cell motility in nasopharyngeal carcinoma.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2022)
Article
Medicine, General & Internal
Muhammad Awawda, Saeed Salman, Salem Billan
Summary: This study investigated the role of human papillomavirus (HPV) in patients with nasopharyngeal carcinoma (NPC) treated with radiotherapy. The results showed that 13.5% of patients had HPV infection, which was not associated with age or lymph node metastasis, but had no prognostic significance.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Oncology
Baoyu He, Hongli Pan, Fengque Zheng, Saiqiong Chen, Qingli Bie, Jinghe Cao, Rou Zhao, Jing Liang, Li Wei, Jianchao Zeng, Hui Li, Xing Cui, Yixuan Ding, Wei Chao, Tiantian Xiang, Yuhe Cheng, Gui Qiu, Shishun Huang, Libo Tang, Jiansheng Chang, Delan Luo, Jie Yang, Bin Zhang
Summary: In this study, a metabolism-related lncRNA, LINC00930, was identified and found to be upregulated and associated with tumorigenesis, metastasis, and poor prognosis in nasopharyngeal carcinoma (NPC). Functionally, LINC00930 promoted glycolysis activity and cell proliferation. Mechanistically, LINC00930 regulated the expression of PFKFB3 through epigenetic modifications. Clinically, targeting LINC00930 and PFKFB3 in combination with radiotherapy induced tumor regression.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Pathology
Changwen Zhai, Cuncun Yuan, Ji Sun, Wanjing Song, Shuyi Wang, Lan Lin
Summary: This study evaluated the clinicopathologic features, molecular characteristics, treatment strategy, and prognosis of nasopharyngeal hyalinizing clear cell carcinoma (HCCC). The study found the clinical features and molecular markers of HCCC, as well as wide local excision as the optimal treatment choice.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2023)
Article
Oncology
Cinzia Bersani, Michael Mints, Nikolaos Tertipis, Linnea Haeggblom, Anders Nasman, Mircea Romanitan, Tina Dalianis, Torbjorn Ramqvist
Article
Immunology
Kurt Arkestal, Michael Mints, Anders Enocson, Ludvig Linton, Per Marits, Hans Glise, John Andersson, Ola Winqvist
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2018)
Article
Orthopedics
Lars Korsnes, Andreas Gottvall, Christian Buttazzoni, Michael Mints
Article
Multidisciplinary Sciences
Stefanie Hiltbrunner, Michael Mints, Maria Eldh, Robert Rosenblatt, Benny Holmstrom, Farhood Alamdari, Markus Johansson, Rosanne E. Veerman, Ola Winqvist, Amir Sherif, Susanne Gabrielsson
SCIENTIFIC REPORTS
(2020)
Article
Otorhinolaryngology
David Landin, Andreas Ahrlund-Richter, Leila Mirzaie, Michael Mints, Anders Nasman, Aeneas Kolev, Linda Marklund, Tina Dalianis, Eva Munck-Wikland, Torbjorn Ramqvist
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2020)
Article
Immunology
David Krantz, Michael Mints, Malin Winerdal, Katrine Riklund, Dorothea Rutishauser, Roman Zubarev, Amir Ali Zirakhzadeh, Farhood Alamdari, Markus Johansson, Amir Sherif, Ola Winqvist
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Michael Mints, David Landin, Anders Nasman, Leila Mirzaie, Ramona Gabriela Ursu, Mark Zupancic, Linda Marklund, Tina Dalianis, Eva Munck-Wikland, Torbjoern Ramqvist
Summary: The study identified a subset of HPV-negative HPSCC patients with favorable prognosis, showing gene expression signature characterized by overexpression of calgranulins and immune genes. High immune activity was found to be strongly related to survival in HPV-negative HPSCC, indicating a potential benefit of immunotherapy. Low expression of HLA class I and S100A12 could potentially be used to select patients for local surgery.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Zongtai Qi, Yating Liu, Michael Mints, Riley Mullins, Reilly Sample, Travis Law, Thomas Barrett, Angela L. Mazul, Ryan S. Jackson, Stephen Y. Kang, Patrik Pipkorn, Anuraag S. Parikh, Itay Tirosh, Joseph Dougherty, Sidharth V. Puram
Summary: Tumors are composed of diverse cells rather than a uniform ball of cells. Recent studies have shown that deconvolution algorithms can be used to computationally estimate cell-type proportions from gene expression data, although their performance in tumor tissues remains unclear.
Article
Oncology
Maria Eldh, Michael Mints, Stefanie Hiltbrunner, Sam Ladjevardi, Farhood Alamdari, Markus Johansson, Tomasz Jakubczyk, Rosanne E. Veerman, Ola Winqvist, Amir Sherif, Susanne Gabrielsson
Summary: The study investigated the release of exosomes from urinary bladder tissue post-TUR-B in UBC patients to find cancer-promoting exosomes originating from both tumour-proximal and tumour-distant sites, highlighting the need for early radical cystectomy. This suggests that the entire bladder tissue may release exosomes contributing to metastasis despite the absence of detectable tumour, supporting the role of exosomes as potential biomarkers and mediators of metastatic spread. Additionally, the enrichment of cancer-related pathways in tissue-derived exosomes from both sites strengthens the hypothesis and emphasizes the necessity for early removal of the bladder in UBC patients.
Article
Oncology
Andreas aehrlund-Richter, Stefan Holzhauser, Tina Dalianis, Anders Naesman, Michael Mints
Summary: Through whole-exome sequencing of primary tumors and relapses in HPV+ TSCC/BOTSCC patients, we identified a specific CDC27 deletion as a potential marker for recurrence and highlighted the significance of mutated genes like BCLAF1 and AQP7 for further investigation.
Article
Multidisciplinary Sciences
Avishai Gavish, Michael Mints, Alissa C. Greenwald, Rouven Hoefflin, Dor Simkin, Roi Tschernichovsky, Noam Galili Darnell, Einav Somech, Chaya Barbolin, Tomer Antman, Daniel Kovarsky, Thomas Barrett, L. Nicolas Gonzalez Castro, Debdatta Halder, Rony Chanoch-Myers, Julie Laffy, Michael Mints, Adi Wider, Rotem Tal, Avishay Spitzer, Toshiro Hara, Maria Raitses-Gurevich, Chani Stossel, Talia Golan, Amit Tirosh, Mario L. Suva, Sidharth V. Puram, Itay Tirosh
Summary: This study curated and integrated data from 77 studies to analyze transcriptional intratumor heterogeneity (ITH) patterns across 1,163 tumor samples, identifying 41 consensus meta-programs and mapping them into 11 hallmarks of transcriptional ITH. The meta-programs cover diverse cellular processes and most of them are similar to those found in non-malignant cells.
Article
Genetics & Heredity
Sidharth V. Puram, Michael Mints, Ananya Pal, Zongtai Qi, Ashley Reeb, Kyla Gelev, Thomas F. Barrett, Sophie Gerndt, Ping Liu, Anuraag S. Parikh, Salma Ramadan, Travis Law, Edmund A. Mroz, James W. Rocco, Doug Adkins, Wade L. Thorstad, Hiram A. Gay, Li Ding, Randal C. Paniello, Patrik Pipkorn, Ryan S. Jackson, Xiaowei Wang, Angela Mazul, Rebecca Chernock, Jose P. Zevallos, Jessica Silva-Fisher, Itay Tirosh
Summary: Single-cell analysis reveals high levels of inter- and intratumoral heterogeneity in head and neck squamous cell carcinoma (HNSCC), specifically in human papillomavirus (HPV)-positive and HPV-negative oropharynx tumors. Divergent patterns of HPV gene expression within HPV-positive tumors have functional effects on treatment resistance.
Meeting Abstract
Medicine, General & Internal
Michael Mints, Kurt Arkestal, Anders Enocson, Ludvig Linton, Per Marits, Hans Glise, John Andersson, Ola Winqvist
SWISS MEDICAL WEEKLY
(2018)
Meeting Abstract
Medicine, General & Internal
Michael Mints, Lars Korsnes, Andreas Gottvall, Christian Buttazzoni
SWISS MEDICAL WEEKLY
(2018)
