Article
Food Science & Technology
Araceli Rivera-Perez, Roberto Romero-Gonzalez, Antonia Garrido Frenich
Summary: An untargeted metabolomics approach using UHPLC-HRMS technology was applied to study metabolic differences of black pepper from different geographical origins and processing methods. PCA and OPLSDA were utilized for sample clustering and discrimination, with 8 potential differential metabolites identified. The NMR-UHPLC-GC mid-level fused model showed excellent performance in geographical and processing discrimination.
FOOD RESEARCH INTERNATIONAL
(2021)
Review
Chemistry, Analytical
Guadalupe Garcia-Llatas, Amparo Alegria, Reyes Barbera, Antonio Cilla
Summary: Plant sterols are bioactive compounds found in all vegetable foods, with potential beneficial effects on lowering cholesterol levels. Analytical methods are needed to assess their health benefits and future applications. Current methodologies include extraction and analysis, with mass spectrometry widely used for its sensitivity and selectivity.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Kerri M. Smith, Ian D. Wilson, Paul D. Rainville
Summary: The described method uses mixed-mode reversed-phase chromatography on a CSH Phenyl-Hexyl column for the analysis of organic acids, showing good resolution and retention time reproducibility. When applied to comparative metabolomic analysis of urine samples from healthy controls and breast cancer positive subjects, the method demonstrated excellent performance in distinguishing between the two groups.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Ewelina Patyra, Krzysztof Kwiatek
Summary: Green chemistry aims to reduce or eliminate the use of hazardous chemicals, with micellar liquid chromatography as a branch that provides advantages for analyzing compounds in various samples.
JOURNAL OF SEPARATION SCIENCE
(2021)
Review
Chemistry, Analytical
Katia Arena, Filippo Mandolfino, Francesco Cacciola, Paola Dugo, Luigi Mondello
Summary: Multidimensional liquid chromatography has become widely used in separating non-volatile analytes in complex matrices, with the main advantage being the enhancement of separation power and peak capacity, and the reduction of co-elutions for more accurate quantitative analysis.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Edith Sandstroem, Chiara Vettorazzo, C. Logan Mackay, Lore G. Troalen, Alison N. Hulme
Summary: A DESI source was built and attached to a Bruker 7T SolariX FT-ICR-MS for in situ analysis of early synthetic dyestuffs. Optimization experiments determined the best conditions for analysis, which were then applied to modern and historical samples. The study demonstrated the applicability and potential of DESI-MS in the field of historical dye analysis, revealing the presence of known degradation products and byproducts in historical samples.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Hyoyoung Kim, Eun Joo Baek, Yong-Kyoung Kim, Hyejin Park, Suel Hye Hur, Jang-Eok Kim, Ho Jin Kim
Summary: In this study, a specific, reliable, and suitable method for a fast and simultaneous analysis of 335 pesticides in ginseng was developed using liquid chromatography quadrupole mass spectrometry (LC-MS/MS) and gas chromatography quadrupole mass spectrometry (GC-MS/MS). The method was validated for linearity, sensitivity, selectivity, accuracy, and precision, with limits of detection (LOD) and limits of quantification (LOQ) ranging from 0.1-5.8 μg/kg and 0.3-17.5 μg/kg, respectively. Analysis of 467 ginseng samples from 2016 to 2019 revealed pesticide residues in 304 samples, but most were below the standard. The hazard quotient (HQ) of ginseng for detected pesticides was less than 1, indicating low risk.
Review
Chemistry, Analytical
Brett Greer, Olivier Chevallier, Brian Quinn, Luis M. Botana, Christopher T. Elliott
Summary: With laboratories seeking to expand analytical capabilities, there has been a shift towards generic sample clean-up techniques like dilute-and-shoot. This methodology is simple, versatile, and facilitates high sample throughput and analysis of various analyte classes.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Xiaoying Chen, Xiaoyu Peng, Xian Sun, Lina Pan, Jiachen Shi, Yu Gao, Yuanluo Lei, Fan Jiang, Ruizhi Li, Yuanfa Liu, Yong-Jiang Xu
Summary: Phospholipids are critical lipids in milk, and a rapid and accurate method for measuring a wide spectrum of phospholipids has been developed in this study. A total of 150 phospholipids were identified, including rarely reported phospholipids containing ethers or vinyl ethers, which could be used as potential markers to distinguish sheep milk varieties and origins.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemical Research Methods
Tim Hammerschick, Walter Vetter
Summary: Centrifugal partition chromatography (CPC) and countercurrent chromatography (CCC) are two preparative techniques used for the isolation and purification of natural products. This study successfully combined the advantages of both techniques by directly linking CPC and CCC, and demonstrated the effectiveness of the hybrid system.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Anna Kubiak, Marcin Mackiewicz, Marcin Karbarz, Magdalena Biesaga
Summary: This study developed a new type of microgel for the removal of bisphenols from aqueous medium. By using microgel solid-phase extraction procedure, the detection limits of bisphenols in liquid food samples can be significantly reduced, and good recoveries and precision can be achieved with a small amount of microgel.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemical Research Methods
Sylwia Studzinska, Ewelina Zawadzka, Szymon Bocian, Michal Szumski
Summary: The research focused on synthesizing and applying an oligonucleotide-based stationary phase for analyzing unmodified and antisense oligonucleotides in different liquid chromatography modes. Results showed that the stationary phase can be used in hydrophilic interaction liquid chromatography and ion exchange chromatography, but achieved better specificity in affinity chromatography.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Cemile Yilmaz, Vural Gokmen
Summary: The article discusses neuroactive compounds in foods and their roles in health and mood, highlighting methods for analyzing these compounds and the potential for designing novel foods containing them.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Tingting Han, Hailin Cong, Bing Yu, Youqing Shen
Summary: This article provides a historical review of the developments and applications of liquid chromatography-mass spectrometry (LC-MS) technology in the detection of peptide biomarkers over the past 10 years.
Review
Chemistry, Analytical
Nicola Sdrigotti, Maurine Collard, Giorgia Purcaro
Summary: The paper explores the interrelationship between hyphenated techniques and mineral oil analysis, highlighting how their mutual interaction has driven technological advancements and knowledge growth, supporting the complex task of mineral oil determination in food.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Food Science & Technology
Ane Arrizabalaga-Larranaga, Guillem Campmajo, Javier Saurina, Oscar Nunez, Francisco Javier Santos, Encarnacion Moyano
Summary: The study used the UHPLC-APCI-HRMS method for the geographical origin authentication of paprika based on capsaicinoids and carotenoids determination, achieving an authentication rate of 80.9%.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Oscar Vidal-Casanella, Javier Moreno-Merchan, Merce Granados, Oscar Nunez, Javier Saurina, Sonia Sentellas
Summary: This study estimated the total polyphenol content and antioxidant capacity in various food and nutraceutical samples using antioxidant assays and chromatographic analysis. The results showed that HPLC fingerprints were suitable for classifying samples based on their nature, and the chromatographic data were correlated with antioxidant data. Regression models based on chromatographic data demonstrated good prediction capacities in estimating the antioxidant activity.
Article
Chemistry, Applied
Guillem Campmajo, Javier Saurina, Oscar Nunez
Summary: This study utilizes flow injection analysis coupled to high-resolution mass spectrometry to address food fraud issues, successfully identifying the geographical origin and quality of Spanish red wines, European paprikas, olive oil, and other vegetable oils with excellent classification accuracies through external validation. High-resolution mass spectrometry allowed for sample matrix characterization by identifying common ions.
Article
Food Science & Technology
Ancuta Nartea, Paolo Lucci, Monica Rosa Loizzo, Rosa Tundis, Mariarosaria Leporini, Luigia Gervasi, Benedetta Fanesi, Oscar Nunez, Natale Giuseppe Frega, Dennis Fiorini, Deborah Pacetti
Summary: The present study aimed to assess the phytochemical content and in vitro bioactivity of ethanolic extracts of Arabica (A) and/or Robusta (R) coffee powder having different geographical origins. The results showed that the qualitative-quantitative composition of the extracts is strongly associated not only with the variety but also with the geographical origin of the samples.
ITALIAN JOURNAL OF FOOD SCIENCE
(2022)
Article
Chemistry, Analytical
Oscar Vidal-Casanella, Kevin Arias-Alpizar, Oscar Nunez, Javier Saurina
Summary: This study developed a new method for determining the content of flavanols in nutraceutical samples. The method involved solid-liquid extraction and liquid chromatography analysis. The results showed that the compositional profiles of selected flavanols were sufficient for describing and discriminating different samples, with cranberry samples being the richest in flavanols and artichoke samples being the least rich.
Editorial Material
Food Science & Technology
Ian Southwell, Oscar Nunez
Article
Food Science & Technology
Monica Vila, Alex Bedmar, Javier Saurina, Oscar Nunez, Sonia Sentellas
Summary: In this study, a non-targeted FIA-MS fingerprinting technique was used to characterize and classify tea and chicory. The results showed that FIA-MS fingerprints were excellent sample descriptors for discriminating between tea and chicory, as well as classifying and distinguishing among some tea groups. The use of PLS also yielded satisfactory results for the detection and quantitation of chicory adulteration in tea. Therefore, the non-targeted FIA-MS fingerprinting technique is a high-throughput, cost-effective, simple, and reliable approach for assessing tea authentication issues.
Article
Food Science & Technology
Victor Garcia-Seval, Claudia Martinez-Alfaro, Javier Saurina, Oscar Nunez, Sonia Sentellas
Summary: Two non-targeted fingerprinting approaches using high performance liquid chromatography with ultraviolet detection were evaluated to characterize, classify, and authenticate honey samples. The results showed that the off-line solid-phase extraction HPLC-UV polyphenolic fingerprints had superior performance in discriminating different types of honey samples.
Article
Chemistry, Applied
Guillem Campmajo, Javier Saurina, Oscar Nunez, Sonia Sentellas
Summary: In this study, the potential of using differential mobility spectrometry coupled to mass spectrometry (DMS-MS) for food classification and authentication was explored. By analyzing 70 Spanish paprika samples, complete classification based on geographical origin and excellent authentication of La Vera and Mallorca samples were achieved.
Article
Food Science & Technology
Ancuta Nartea, Benedetta Fanesi, Alessandra Giardinieri, Guillem Campmajo, Paolo Lucci, Javier Saurina, Deborah Pacetti, Dennis Fiorini, Natale Giuseppe Frega, Oscar Nunez
Summary: The impact of different cooking methods on the phytochemical profiles of orange and violet cauliflower was investigated. The study found that the cooking methods significantly influenced the distribution and content of various compounds in the cauliflower samples. Furthermore, the impact of the cooking techniques varied depending on the type of cauliflower.
Article
Biochemistry & Molecular Biology
Victor Garcia-Seval, Javier Saurina, Sonia Sentellas, Oscar Nunez
Summary: The study evaluated the feasibility of non-targeted off-line SPE LC-LRMS polyphenolic fingerprints in classifying and authenticating Spanish honey samples based on their botanical origin and geographical production region. The results demonstrated that the method was able to differentiate blossom-honey and honeydew-honey samples and extract characteristic compounds of different botanical varieties.
Article
Biochemistry & Molecular Biology
Victor Garcia-Seval, Javier Saurina, Sonia Sentellas, Oscar Nunez
Summary: A non-targeted LC-HRMS fingerprinting methodology was developed to characterize and classify Spanish honey samples. The methodology demonstrated superior classification and discrimination capability, allowing for the authentication of honey samples based on their botanical origins as well as the classification of their production region.
Article
Biochemistry & Molecular Biology
Monica Fernandez-Estelle, Victor Hernandez-Gonzalez, Javier Saurina, Oscar Nunez, Sonia Sentellas
Summary: Honey is appreciated for its nutritional properties and health benefits, including antioxidant, anti-inflammatory, antifungal, and antibacterial activities. The composition of each honey variety, with botanical origin as a distinctive feature, determines these attributes. This study determined the antioxidant capacity, phenolic compound content, and flavonoid content of different honeys, finding that honeydew honeys had higher oxidizable species content and stronger antioxidant capacity. Using classification models, honeydew and blossom honeys were successfully discriminated.
Article
Food Science & Technology
Thom Romers, Javier Saurina, Sonia Sentellas, Oscar Nunez
Summary: In order to prevent tea fraud, a HPLC-UV method was proposed to classify and authenticate tea samples. By measuring the content of polyphenolic and phenolic acid compounds in tea and using principal component analysis and partial least squares-discriminant analysis, tea samples were effectively distinguished from adulterants like chicory. Furthermore, this method could be used to detect and quantify adulteration of different tea varieties, with high accuracy and stability.
Article
Food Science & Technology
Guillem Campmajo, Javier Saurina, Oscar Nunez
Summary: The study evaluated the metabolomic diversity of ten different nut types using non-targeted liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). A multi-class model with a classification accuracy of 100% was developed using LC-HRMS fingerprints and partial least squares regression-discriminant analysis (PLS-DA). Discriminant compounds were selected based on variable importance in projection (VIP) scores obtained from specific nut type versus other samples models and were annotated/identified using HRMS data. Adulteration of almond-based products with hazelnut or peanut was successfully detected and quantified through a targeted LC-HRMS study using some of the identified markers and partial least squares (PLS) regression.
