Article
Biochemistry & Molecular Biology
Janna N. Schultzhaus, William Judson Hervey, Chris R. Taitt, Chris R. So, Dagmar H. Leary, Kathryn J. Wahl, Christopher M. Spillmann
Summary: Barnacles are of interest to the scientific community due to their unique evolutionary trajectory, vast diversity, and economic impact. Their adhesion enables them to exist as sessile adults and colonize oceans globally. By comparing the adhesive proteomes of different barnacle species, it is possible to identify similarities and differences, providing insights for future research on functional differences across the barnacle tree of life.
Article
Biochemistry & Molecular Biology
Dany Dominguez-Perez, Daniela Almeida, Josef Wissing, Andre M. Machado, Lothar Jansch, Agostinho Antunes, Luis Filipe Castro, Vitor Vasconcelos, Alexandre Campos, Isabel Cunha
Summary: This study focused on the adhesive system of the stalked goose barnacle L. anatifera, identifying a variety of proteins including cement specific adhesive proteins, proteases, protease inhibitors, and other structural proteins. Through shotgun proteomic analysis, the researchers identified and quantified peptide sequences in the adhesive gland and cement, providing insight into the composition of the adhesive proteins. The study also identified unannotated proteins with similar properties to known CPs and demonstrated the importance of Principal Components Analysis in identifying non-conserved adhesive proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Simin Masoudi, Mark Willcox
Summary: The aim of this study was to evaluate a new digestion method to quantify protein deposition on contact lenses. The results showed that immobilized trypsin can effectively digest protein deposits from the surface of contact lenses, and the ability to analyze surface protein concentrations may help in understanding the impact of surface deposition on clinical outcomes.
Article
Chemistry, Analytical
Andreas Dannhorn, Stephanie Ling, Steven Powell, Eileen McCall, Gareth Maglennon, Gemma N. Jones, Andrew J. Pierce, Nicole Strittmatter, Gregory Hamm, Simon T. Barry, Josephine Bunch, Richard J. A. Goodwin, Zoltan Takats
Summary: A decontamination approach using UV-C light was presented in this study to inactivate clinically relevant pathogens without causing damage to the tissue metabolome and xenobiotics, allowing for the study of the biodistribution of endogenous metabolites. Additionally, spatially integrated abundances of ATR inhibitor ceralasertib from decontaminated human biopsies were determined using DESI-MSI.
ANALYTICAL CHEMISTRY
(2021)
Article
Plant Sciences
Mayya Razgonova, Yulia Zinchenko, Konstantin Pikula, Lyudmila Tekutyeva, Oksana Son, Alexander Zakharenko, Tatiana Kalenik, Kirill Golokhvast
Summary: This study analyzed the phytochemical substances in grains of Zea mays L. and found that anthocyanins are mainly located in the aleurone layer. A total of 56 compounds, including 30 polyphenols, were identified using tandem mass spectrometry. This method provides an efficient and rapid analysis of anthocyanins distribution in different plants, which is important for food plant breeding.
Article
Optics
Jakob G. P. Lingg, Thomas S. Bischof, Bernardo A. Arus, Emily D. Cosco, Ellen M. Sletten, Christopher J. Rowlands, Oliver T. Bruns, Andriy Chmyrov
Summary: The development of fluorophores with photoemission beyond 1000 nm provides the opportunity to develop novel fluorescence microscopes sensitive to those wavelengths. Imaging at wavelengths beyond the visible spectrum enables imaging depths of hundreds of microns in intact tissue, making this attractive for volumetric imaging applications.
LASER & PHOTONICS REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Tiziano Marzo, Giarita Ferraro, Lorena Maria Cucci, Alessandro Pratesi, Orjan Hansson, Cristina Satriano, Antonello Merlino, Diego La Mendola
Summary: The study shows that the anti-cancer drug oxaliplatin efficiently binds angiogenin, inhibiting its promoting effects on cell proliferation and migration. This suggests angiogenin as a potential target of oxaliplatin, indicating a new mechanism for the anti-neoplastic activity of this platinum drug and opening avenues for novel approaches in combined anti-cancer anti-angiogenic therapy.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Eva Illes-Toth, Oliver J. Hale, James W. Hughes, Nicole Strittmatter, Jonathan Rose, Ben Clayton, Rebecca Sargeant, Stewart Jones, Andreas Dannhorn, Richard J. A. Goodwin, Helen J. Cooper
Summary: This study demonstrates for the first time the in situ mass spectrometry analysis of a non-covalent protein-drug complex formed in vivo, and provides a direct route for target-drug characterization in early stage drug discovery by spatial mapping across liver tissue.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Cristina Manis, Alessia Manca, Antonio Murgia, Giuseppe Uras, Pierluigi Caboni, Terenzio Congiu, Gavino Faa, Antonella Pantaleo, Giacomo Cao
Summary: This study investigated the changes in the structure and function of red blood cells under simulated microgravity. The findings revealed morphological changes, oxidative stress, and alterations in membrane composition. These discoveries are significant for understanding the effects of microgravity on human cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Construction & Building Technology
Lourdes Alameda Cuenca-Romero, Raquel Arroyo, Alvaro Alonso, Sara Gutierrez-Gonzalez, Veronica Calderon
Summary: In accordance with European policies, using roof waste as recyclable materials to replace aggregates in the production of new construction materials shows potential. By modifying the chemical properties of binders and adding surfactants, the mechanical strength and other properties of the materials can be improved. These eco-mortars have good adaptability to temperature and can serve as sustainable alternatives to current materials.
JOURNAL OF BUILDING ENGINEERING
(2022)
Article
Chemistry, Analytical
Holly-May Lewis, Catia Costa, Veronique Dartois, Firat Kaya, Mark Chambers, Janella de Jesus, Vladimir Palitsin, Roger Webb, Melanie J. Bailey
Summary: This study combines elemental imaging and a new method for spatially resolved lipidomics to investigate the relationship between metals, drugs, and lipids in different areas of tissues. The method was applied to rabbit lung tissues with tuberculosis infection and demonstrated the association between ion accumulation, lipid profiles, and local drug distribution.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Yuto Miyoshi, Takahiro Nishimura, Yu Shimojo, Keita Okayama, Kunio Awazu
Summary: A miniaturized endoscopic laser system with laser steering is developed for minimally invasive laser treatment of micro-lesions inside narrow organs. The system uses a single fiber bundle to acquire endoscopic images and modulate laser irradiation simultaneously. The system achieves lesion-selective laser treatment in narrow organs, such as the peripheral lung and coronary arteries, through spatial control of laser steering trajectory and photothermal effects.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Martijn M. Vanduijn, Hendrik-Jan Brouwer, Pablo Sanz de la Torre, Jean-Pierre Chervet, Theo M. Luider
Summary: The research team reported an improved electrochemical method that achieves complete reduction of both intermolecular and intramolecular disulfide bridges in a set of monoclonal antibodies. The method utilizes an online electrochemical flow cell to provide information on the pairing of heavy and light chains in the antibodies. The complete reduction of intramolecular disulfide bridges is important for the intact mass of the antibody chain and the confirmation of the protein's amino acid sequence.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Artur Pinski, David Roujol, Cecile Pouzet, Luc Bordes, Helene San Clemente, Laurent Hoffmann, Elisabeth Jamet
Summary: This article compares the bioinformatics predictions of sub-cellular localization of proteins assumed to be CWPs to mass spectrometry data and checked the sub-cellular localization of certain CWPs with specific features using cell biology methods. The study confirms the prediction of signal peptide length in most cases but is less conclusive for GPI-anchors, and also identifies certain CWPs with double sub-cellular localization and raises questions about the actual anchoring to the plasma membrane for some proteins.
Article
Multidisciplinary Sciences
V. Dumrongprechachan, R. B. Salisbury, G. Soto, M. Kumar, M. L. MacDonald, Y. Kozorovitskiy
Summary: This study employed a genetically targeted APEX2-based proximity labeling approach to uncover proteome dynamics in two classes of striatal spiny projection neurons in the mouse brain. The findings were validated using imaging, electron microscopy, and mass spectrometry, providing important insights for understanding the development and functional organization of neural circuits in the brain.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Applied
Chin-Sing Lim, Gary H. Dickinson, Stacy Sommer, Serena Lay-Ming Teo, Rajan B. Bodkhe, Dean C. Webster, Yong Ying Loo
JOURNAL OF COATINGS TECHNOLOGY AND RESEARCH
(2015)
Article
Biotechnology & Applied Microbiology
Shane J. Stafslien, Stacy Sommer, Dean C. Webster, Rajan Bodkhe, Robert Pieper, Justin Daniels, Lyndsi Vander Wal, Maureen C. Callow, James A. Callow, Emily Ralston, Geoff Swain, Lenora Brewer, Dean Wendt, Gary H. Dickinson, Chin-Sing Lim, Serena Lay-Ming Teo
Article
Biology
Gary H. Dickinson, Xu Yang, Fanghui Wu, Beatriz Orihuela, Dan Rittschof, Elia Beniash
BIOLOGICAL BULLETIN
(2016)
Article
Ecology
William D. Coffey, Jessica A. Nardone, Aparna Yarram, W. Christopher Long, Katherine M. Swiney, Robert J. Foy, Gary H. Dickinson
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
(2017)
Article
Engineering, Ocean
Manuel A. Figueroa, Jennifer D. Schablik, Madison Mastroberte, Lovejot Singh, Gary H. Dickinson
MARINE TECHNOLOGY SOCIETY JOURNAL
(2017)
Article
Biotechnology & Applied Microbiology
Emma Timmins-Schiffman, William D. Coffey, Wilber Hua, Brook L. Nunn, Gary H. Dickinson, Steven B. Roberts
Article
Marine & Freshwater Biology
Brittan V. Steffel, Kathryn E. Smith, Gary H. Dickinson, Jennifer A. Flannery, Kerstin A. Baran, Miranda N. Rosen, James B. McClintock, Richard B. Aronson
INVERTEBRATE BIOLOGY
(2019)
Article
Engineering, Biomedical
Miranda N. Rosen, Kerstin A. Baran, Justin N. Sison, Brittan Steffel, W. Christopher Long, Robert J. Foy, Kathryn E. Smith, Richard B. Aronson, Gary H. Dickinson
ACTA BIOMATERIALIA
(2020)
Article
Multidisciplinary Sciences
Rebecca A. Metzler, Jessica O'Malley, Jack Herrick, Brett Christensen, Beatriz Orihuela, Daniel Rittschof, Gary H. Dickinson
ROYAL SOCIETY OPEN SCIENCE
(2020)
Article
Biology
Gary H. Dickinson, Shai Bejerano, Trina Salvador, Christine Makdisi, Shrey Patel, W. Christopher Long, Katherine M. Swiney, Robert J. Foy, Brittan Steffel, Kathryn E. Smith, Richard B. Aronson
Summary: The study found that ocean acidification can have specific effects on the exoskeleton of female southern Tanner crabs, including reduced microhardness, changes in elemental content, structural integrity, and phase of calcium carbonate. These effects varied in different body regions and highlight the vulnerability of long-lived crabs like C. bairdi to ocean acidification due to limited capacity for repair.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Patricia K. Thomas, Lindsay K. Sullivan, Gary H. Dickinson, Catherine M. Davis, Anthony G. Lau
Summary: The study found that exposure to helium-4 radiation caused significant changes in bone material properties in rats, with a significant decline in shear modulus at 25 cGy exposure. However, there was a recovery of moduli by 180 days after exposure, indicating a potential biological mechanism at play in bone formation and turnover affected by radiation exposure.
CALCIFIED TISSUE INTERNATIONAL
(2021)
Review
Ecology
Kyle R. Siegel, Muskanjot Kaur, A. Calvin Grigal, Rebecca A. Metzler, Gary H. Dickinson
Summary: This study provides a systematic review and meta-analysis on the effects of ocean acidification on the exoskeleton of crustaceans. The results suggest that ocean acidification can affect the ion content and functional properties of crustacean exoskeleton, but the effects vary depending on seawater pCO(2) and species identity. The findings highlight the potential vulnerability of crustacean exoskeleton to ocean acidification and call for further research considering the variability of crustacean responses and ecologically relevant pCO(2) conditions.
ECOLOGY AND EVOLUTION
(2022)
Article
Marine & Freshwater Biology
Tait Algayer, Ahmed Mahmoud, Sanjana Saksena, W. Christopher Long, Katherine M. M. Swiney, Robert J. J. Foy, Brittan V. V. Steffel, Kathryn E. E. Smith, Richard B. B. Aronson, Gary H. H. Dickinson
Summary: The structural and mechanical properties of decapod exoskeleton have important effects on foraging, defense, and locomotion. Ocean acidification poses a threat to calcifying organisms, including the commercially important Chionecetes opilio. However, the vulnerability of C. opilio to ocean acidification has not been explored. In this study, we examined the long-term effects of decreased seawater pH on the exoskeletal properties of adult C. opilio, and found that the exoskeletons were robust to ocean acidification regardless of pH.
Article
Environmental Sciences
Jessica A. Nardone, Shrey Patel, Kyle R. Siegel, Dana Tedesco, Conall G. McNicholl, Jessica O'Maney, Jack Herrick, Rebecca A. Metzler, Beatriz Orihuela, Daniel Rittschof, Gary H. Dickinson
FRONTIERS IN MARINE SCIENCE
(2018)