Journal
BMC MICROBIOLOGY
Volume 15, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12866-015-0474-9
Keywords
Enterovirus 71; Mouse model; Vaccine candidate
Categories
Funding
- Chinese Ministry of Science and Technology [2012CB911100, 2013ZX10001-005]
- Chinese Ministry of Education [IRT1016]
- National Natural Science Foundation of China [31270202]
- Key Laboratory of Molecular Virology, Jilin Province [20102209]
- Health and Family Planning Commission of Jilin Province [2013Z066]
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Background: Circulating enterovirus 71 (EV-A71)-associated hand, foot, and mouth disease is on the rise in the Asian-Pacific region. Although animal models have been developed using mouse-adapted EV-A71 strains, mouse models using primary EV-A71 isolates are scarce. Lethal animal models with circulating EV-A71 infection would contribute to studies of pathogenesis as well as vaccine development and evaluation. Results: In this study, we established a lethal mouse model using primary EV-A71 isolates from patients infected with serotypes that are currently circulating in humans. We also characterized the dose-dependent virulence and pathologic changes of circulating EV-A71 in this mouse model. Most importantly, we have established this mouse model as a suitable system for EV-A71 vaccine evaluation. An inactivated EV-A71 vaccine candidate offered complete protection from death induced by various circulating EV-A71 viruses to neonatal mice that were born to immunized female mice. The sera of the immunized dams and their pups showed higher neutralization titers against multiple circulating EV-A71 viruses. Conclusions: Thus, our newly established animal model using primary EV-A71 isolates is helpful for future studies on viral pathogenesis and vaccine and drug development.
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