Integration of Genetic and Process Engineering for Optimized Rhamnolipid Production Using Pseudomonas putida

Exploiting the Natural Diversity of RhlA Acyltransferases for the Synthesis of the Rhamnolipid Precursor 3-(3-Hydroxyalkanoyloxy)Alkanoic Acid

(2020) Andrea Germer et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Comparison of Three Xylose Pathways in Pseudomonas putida KT2440 for the Synthesis of Valuable Products

(2020) Isabel Bator et al.   Frontiers in Bioengineering and Biotechnology

Development, evaluation, and optimisation of downstream process concepts for rhamnolipids and 3-(3-hydroxyalkanoyloxy)alkanoic acids

(2020) Andreas Biselli et al.   SEPARATION AND PURIFICATION TECHNOLOGY

Characterization of Context-Dependent Effects on Synthetic Promoters

(2020) Sebastian Köbbing et al.   Frontiers in Bioengineering and Biotechnology

Killing Two Birds With One Stone – Strain Engineering Facilitates the Development of a Unique Rhamnolipid Production Process

(2020) Isabel Bator et al.   Frontiers in Bioengineering and Biotechnology

Engineering of natural product biosynthesis in Pseudomonas putida

(2020) Anita Loeschcke et al.   CURRENT OPINION IN BIOTECHNOLOGY

Growth of engineered Pseudomonas putida KT2440 on glucose, xylose and arabinose: hemicellulose hydrolysates and their major sugars as sustainable carbon sources

(2019) Yan Wang et al.   Global Change Biology Bioenergy

Synthetic biology approaches for chromosomal integration of genes and pathways in industrial microbial systems

(2019) Lei Li et al.   BIOTECHNOLOGY ADVANCES

Semi‐rational evolution of the 3‐(3‐hydroxyalkanoyloxy)alkanoate ( HAA ) synthase RhlA to improve rhamnolipid production in Pseudomonas aeruginosa and Burkholderia glumae

(2019) Carlos Eduardo Dulcey et al.   FEBS Journal

Pseudomonas putida rDNA is a favored site for the expression of biosynthetic genes

(2019) Andreas Domröse et al.   Scientific Reports

Heterologous rhamnolipid biosynthesis by P. putida KT2440 on bio-oil derived small organic acids and fractions

(2019) Stefanie Arnold et al.   AMB Express

Protocols for yTREX /Tn5‐based gene cluster expression in Pseudomonas putida

(2019) Robin Weihmann et al.   Microbial Biotechnology

Scale‐up of a type I secretion system in E. coli using a defined mineral medium

(2019) Nina Ihling et al.   BIOTECHNOLOGY PROGRESS

Engineered Rhodobacter capsulatus as a Phototrophic Platform Organism for the Synthesis of Plant Sesquiterpenoids

(2019) Katrin Troost et al.   Frontiers in Microbiology

Primer3_masker: integrating masking of template sequence with primer design software

(2018) Triinu Kõressaar et al.   BIOINFORMATICS

Online measurement of the respiratory activity in shake flasks enables the identification of cultivation phases and patterns indicating recombinant protein production in various Escherichia coli host strains

(2018) Nina Ihling et al.   BIOTECHNOLOGY PROGRESS

Gate-to-gate life cycle assessment of biosurfactants and bioplasticizers production via biotechnological exploitation of fats and waste oils

(2018) Alexandros Kopsahelis et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Metabolic engineering of Pseudomonas taiwanensis VLB120 with minimal genomic modifications for high-yield phenol production

(2018) Benedikt Wynands et al.   METABOLIC ENGINEERING

Prediction of recombinant protein production by Escherichia coli derived online from indicators of metabolic burden

(2018) Martina Julia Mühlmann et al.   BIOTECHNOLOGY PROGRESS

Production of rhamnolipids by integrated foam adsorption in a bioreactor system

(2018) Iva Anic et al.   AMB Express

A new approach for downstream purification of rhamnolipid biosurfactants

(2018) Krutika Invally et al.   FOOD AND BIOPRODUCTS PROCESSING

Foam adsorption as an ex situ capture step for surfactants produced by fermentation

(2017) Iva Anic et al.   JOURNAL OF BIOTECHNOLOGY

Optimizing recombinant protein expression via automated induction profiling in microtiter plates at different temperatures

(2017) Martina Mühlmann et al.   Microbial Cell Factories

Production of microbial biosurfactants: Status quo of rhamnolipid and surfactin towards large-scale production

(2017) Marius Henkel et al.   Biotechnology Journal

Prediction of Escherichia coli expression performance in microtiter plates by analyzing only the temporal development of scattered light during culture

(2017) Tobias Ladner et al.   Journal of Biological Engineering

Novel insights into biosynthesis and uptake of rhamnolipids and their precursors

(2016) Andreas Wittgens et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Correlation for the maximum oxygen transfer capacity in shake flasks for a wide range of operating conditions and for different culture media

(2016) Kristina Meier et al.   BIOCHEMICAL ENGINEERING JOURNAL

High performance liquid chromatography-charged aerosol detection applying an inverse gradient for quantification of rhamnolipid biosurfactants

(2016) Beate Behrens et al.   JOURNAL OF CHROMATOGRAPHY A

Biofilm as a production platform for heterologous production of rhamnolipids by the non-pathogenic strain Pseudomonas putida KT2440

(2016) Vinoth Wigneswaran et al.   Microbial Cell Factories

Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

(2016) Georg Wandrey et al.   Microbial Cell Factories

Broad-Host-Range ProUSER Vectors Enable Fast Characterization of Inducible Promoters and Optimization of p-Coumaric Acid Production in Pseudomonas putida KT2440

(2016) Patricia Calero et al.   ACS Synthetic Biology

Development of a Cradle-to-Grave Approach for Acetylated Acidic Sophorolipid Biosurfactants

(2016) Niki Baccile et al.   ACS Sustainable Chemistry & Engineering

Engineering mediator-based electroactivity in the obligate aerobic bacterium Pseudomonas putida KT2440

(2015) Simone Schmitz et al.   Frontiers in Microbiology

Efficient recombinant production of prodigiosin in Pseudomonas putida

(2015) Andreas Domröse et al.   Frontiers in Microbiology

The Functional Structure of Central Carbon Metabolism in Pseudomonas putida KT2440

(2014) Suresh Sudarsan et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A Stereospecific Pathway Diverts β-Oxidation Intermediates to the Biosynthesis of Rhamnolipid Biosurfactants

(2014) Ahmad Mohammad Abdel-Mawgoud et al.   CHEMISTRY & BIOLOGY

The metabolic cost of flagellar motion inPseudomonas putida KT2440

(2013) Esteban Martínez-García et al.   ENVIRONMENTAL MICROBIOLOGY

Life cycle assessment of surfactants: the case of an alkyl polyglucoside used as a self emulsifier in cosmetics

(2013) Jérôme Guilbot et al.   GREEN CHEMISTRY

Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440

(2012) Andreas Wittgens et al.   Microbial Cell Factories

Microbial biosurfactants: challenges and opportunities for future exploitation

(2012) Roger Marchant et al.   TRENDS IN BIOTECHNOLOGY

Response of Pseudomonas putida KT2440 to Increased NADH and ATP Demand

(2011) Birgitta E. Ebert et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Controlling pH in shake flasks using polymer-based controlled-release discs with pre-determined release kinetics

(2011) Marco Scheidle et al.   BMC BIOTECHNOLOGY

Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440

(2011) Esteban Martínez-García et al.   ENVIRONMENTAL MICROBIOLOGY

Ultrafiltrative separation of rhamnolipid from culture medium

(2011) Anna Witek-Krowiak et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Comparative transcriptomics and proteomics of p-hydroxybenzoate producing Pseudomonas putida S12: novel responses and implications for strain improvement

(2010) Suzanne Verhoef et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Pseudomonas aeruginosa PAO1 as a model for rhamnolipid production in bioreactor systems

(2010) Markus Michael Müller et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

ILCD Handbook Public Consultation Workshop

(2010) David W. Pennington et al.   INTERNATIONAL JOURNAL OF LIFE CYCLE ASSESSMENT

Towards Life Cycle Sustainability Assessment

(2010) Matthias Finkbeiner et al.   Sustainability

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

(2009) S. A. Bustin et al.   CLINICAL CHEMISTRY