Article
Biochemistry & Molecular Biology
Hyun Jun Woo, Ji Yeong Yang, Pyeongjae Lee, Jong-Bae Kim, Sa-Hyun Kim
Summary: The natural compound zerumbone extracted from Zingiber zerumbet Smith was found to inhibit the urease activity of H. pylori by forming dimers, trimers, or tetramers, suggesting its potential as a therapeutic agent for treating H. pylori infections in humans.
Article
Gastroenterology & Hepatology
Qinbo Cai, Peng Shi, Yujie Yuan, Jianjun Peng, Xinde Ou, Wen Zhou, Jin Li, Taiqiang Su, Liangliang Lin, Shirong Cai, Yulong He, Jianbo Xu
Summary: The study revealed a new mechanism of H pylori-induced atrophic gastritis through CXCR2-mediated cellular senescence. Inhibition of CXCR2 signaling is suggested as a potential preventive therapy for targeting H pylori-induced atrophic gastritis.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Plant Sciences
Marina R. Wylie, Ian H. Windham, Faith C. Blum, Hannah Wu, D. Scott Merrell
Summary: Nimbolide, a compound found in neem tree extract, shows significant bactericidal activity against Helicobacter pylori, making it a potential future treatment for H. pylori infection.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ji Yeong Yang, Jong-Bae Kim, Pyeongjae Lee, Sa-Hyun Kim
Summary: Evodiamine inhibits the growth of H. pylori by downregulating gene expressions related to replication and transcription machineries of the bacteria. Moreover, evodiamine also suppresses urease expression, leading to reduced translocation of CagA and VacA proteins into AGS cells. Additionally, evodiamine inhibits the activation of signaling proteins induced by H. pylori infection, contributing to reduction of IL-8 production in AGS cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Sibiao Yue, Lei Wang, George N. DeMartino, FangZhou Zhao, Yi Liu, Matthew H. Sieber
Summary: The regulation of cellular proteostasis is connected to the metabolic state of quiescent cells in vivo. The authors demonstrate that shifts in the ubiquitin-proteome system are linked to mitochondrial metabolic changes and respiratory quiescence. Cellular proteostasis defects and mitochondrial dysfunction play a role in infertility, cancer, and age-related diseases. Through a multi-organism approach, it is shown that dynamic shifts in the ubiquitin proteasome system drive mitochondrial remodeling during cellular quiescence.
NATURE COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Ling Wang, Wen-Qun Li, Fen Liu, Yuan-Jian Li, Jie Du
Summary: This study investigates the variations of xCT activity in the gastric mucosa of patients with Helicobacter pylori (Hp) infection. The results show that patients with Hp infection have reduced xCT expression and lower glutamate concentration in gastric juice. The study also discovers a high expression of microRNAs that negatively regulate xCT expression in Hp-positive patients. The findings suggest that reduced xCT activity may play an important role in gastric ulcers caused by Hp infection and the microRNA/xCT pathway could be a potential treatment target for Hp-infection-related ulcers.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Paola Di Fermo, Silvia Di Lodovico, Emanuela Di Campli, Sara D'Arcangelo, Firas Diban, Simonetta D'Ercole, Mara Di Giulio, Luigina Cellini
Summary: This study evaluated the physiological changes of Helicobacter pylori from active state to viable-but-non-culturable (VBNC) and persister (AP) states, and the ability of vitamin C to interfere with dormancy generation/resuscitation. The results showed that vitamin C reduced the likelihood of H. pylori entering the VBNC state and delayed the entry into the AP state. Vitamin C promoted resuscitation in the VBNC state and reduced the formation of aggregates in the AP state. Pretreatment with vitamin C favored the selection of microbial forms that are more susceptible to H. pylori therapeutic schemes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Jingfei Hu, Xueqing Tian, Tong Wei, Hangjie Wu, Jing Lu, Mingsheng Lyu, Shujun Wang
Summary: The exopolysaccharide (EPS) extracted from Lactobacillus plajomi PW-7 showed antibacterial activity and antioxidant capacity against Helicobacter pylori. It has a potential application in developing EPS-based foods. The structural characteristics and biological activity of EPS play a key role in its potential applications.
Review
Biochemistry & Molecular Biology
Rosanna Capparelli, Domenico Iannelli
Summary: Epigenetics plays a role in regulating gene expression, cell differentiation, and response to environmental stimuli. Helicobacter pylori (Hp) uses DNA methylation to silence genes in human gastric mucosa, which is a primary cause of gastric cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Peter Malfertheiner, M. Constanza Camargo, Emad El-Omar, Jyh-Ming Liou, Richard Peek, Christian Schulz, Stella I. Smith, Sebastian Suerbaum
Summary: Helicobacter pylori infection causes chronic gastritis and can lead to severe gastroduodenal pathologies, including peptic ulcer and gastric cancer. This Primer summarizes the epidemiology, pathophysiology, diagnosis and management of this infection, and discusses patient quality of life and open research questions.
NATURE REVIEWS DISEASE PRIMERS
(2023)
Article
Chemistry, Multidisciplinary
Doha H. Abou Baker, Heba S. Abbas
Summary: Recently, the synthesis of selenium nanoparticles (SeNPs) using plant extracts as a substitute for chemical synthesis has gained attention. In this study, researchers synthesized biogenic SeNPs using pomegranate peels aqueous extract (PPAE) and evaluated their potential against Helicobacter pylori and stomach cancer cells. The results showed that the SeNPs exhibited significant anti-H. pylori activity and dose-dependent inhibition of stomach cancer cell growth. These findings suggest that the biogenic SeNPs have promising therapeutic potential for treating H. pylori infection and stomach cancer.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Jiao Li, Wan Zheng, Ming Gu, Long Han, Yanmei Luo, Koukou Yu, Mengxin Sun, Yuliang Zong, Xiuxiu Ma, Bing Liu, Ethan P. Lowder, Deanna L. Mendez, Robert G. Kranz, Kai Zhang, Jiapeng Zhu
Summary: This study presents high resolution cryo-EM structures of CcmABCD in different states and proposes a hypothetical model of heme trafficking, transfer, and release of holoCcmE.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Medicinal
Marta Maslanka, Wojciech Tabor, Pawel Krzyzek, Agnieszka Grabowiecka, Lukasz Berlicki, Artur Mucha
Summary: Catechols have been identified as potent covalent inhibitors of ureases by modifying cysteine residues at the active site. We synthesized novel catecholic derivatives with carboxylate and phosphonic/phosphinic groups, which demonstrated expanded specific interactions. The compound 2-(3,4-dihydroxyphenyl)-3-phosphonopropionic acid (15) exhibited significant anti-urease potential with a Ki value of 2.36 μM and displayed antiureolytic effects in live Helicobacter pylori cells at a submicromolar concentration (IC50 = 0.75 μM). Molecular modeling revealed its binding to the active site through electrostatic and hydrogen bond interactions. These catecholic phosphonic acids exhibited specific antiureolytic activity without cytotoxicity to eukaryotic cells.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Microbiology
Tran Thi Dieu Thuy, Pei-Yun Kuo, Sha-Ma Lin, Cheng-Yen Kao
Summary: This study evaluated and compared the anti-Helicobacter pylori activity of three potential probiotic strains and found that SLC13 showed the highest adhesion ability and inhibitory activity. It also alleviated the inflammatory response induced by H. pylori infection.
Article
Multidisciplinary Sciences
Tomohiko Yasuda, Hyun Seok Lee, Su Youn Nam, Hiroto Katoh, Yuko Ishibashi, Somay Yamagata Murayama, Hidenori Matsui, Hiroki Masuda, Emiko Rimbara, Nobuyuki Sakurazawa, Hideyuki Suzuki, Hiroshi Yoshida, Yasuyuki Seto, Shumpei Ishikawa, Seong Woo Jeon, Masahiko Nakamura, Sachiyo Nomura
Summary: Genetic analysis and culturing techniques for gastric non-Helicobacter pylori Helicobacter (NHPH) are advancing, with NHPH reported to accompany certain gastric conditions. A study on Korean gastric cancer patients found a low percentage positive for NHPH, suggesting it may play a role in gastric cancer development, albeit with lower pathogenicity compared to H. pylori.
SCIENTIFIC REPORTS
(2022)
Article
Gastroenterology & Hepatology
Maria Kulecka, Barbara Fraczek, Michal Mikula, Natalia Zeber-Lubecka, Jakub Karczmarski, Agnieszka Paziewska, Filip Ambrozkiewicz, Katarzyna Jagusztyn-Krynicka, Pawel Cieszczyk, Jerzy Ostrowski
Article
Infectious Diseases
R. Izdebski, M. Sitkiewicz, P. Urbanowicz, M. Krawczyk, S. Brisse, M. Gniadkowski
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Article
Multidisciplinary Sciences
Paula Roszczenko-Jasinska, Huong N. Vu, Gabriel A. Subuyuj, Ralph Valentine Crisostomo, James Cai, Nicholas F. Lien, Erik J. Clippard, Elena M. Ayala, Richard T. Ngo, Fauna Yarza, Justin P. Wingett, Charumathi Raghuraman, Caitlin A. Hoeber, Norma C. Martinez-Gomez, Elizabeth Skovran
SCIENTIFIC REPORTS
(2020)
Review
Biotechnology & Applied Microbiology
Paula Roszczenko-Jasinska, Marta Ilona Wojtys, Elzbieta K. Jagusztyn-Krynicka
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Infectious Diseases
Teresa Goncalves Ribeiro, Radoslaw Izdebski, Pawel Urbanowicz, Yehuda Carmeli, Marek Gniadkowski, Luisa Peixe
Summary: Strains 6105(T) and 6106 from colonized patients in a hospital in Tel-Aviv, Israel, were identified as a new species of Citrobacter, named C. telavivumsp. nov., showing distinct genetic and biochemical characteristics from other recognized species. They exhibited resistance to various antibiotics, and contained novel antibiotic resistance genes and plasmids.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Microbiology
Pawel Urbanowicz, Ibrahim Bitar, Radoslaw Izdebski, Anna Baraniak, Elzbieta Literacka, Jaroslav Hrabak, Marek Gniadkowski
Summary: The study identified the first VIM-2 metallo-beta-lactamase-producing Pseudomonas aeruginosa isolates with an In4-like integron in three hospitals in Poland from 2003 to 2004. By 2005 to 2015, the MPPA population expanded in the country, with 80 isolates out of 454 having In461 integron, showing significant genetic diversity and only a few small clonal clusters. The large-scale spread of multidrug resistance plasmids in P. aeruginosa populations poses an epidemiological threat and contributes to the knowledge of IncP-2 types.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
M. Biedrzycka, P. Urbanowicz, A. Guzek, S. Brisse, M. Gniadkowski, R. Izdebski
Summary: The study assessed the spread of NDM-1-producing Klebsiella pneumoniae ST147 organisms in Poland since 2015, demonstrating a high degree of homogeneity and close relatedness to organisms spreading in the Mediterranean region. The isolates showed a strict outbreak cluster with minor variations in resistome and plasmid replicon profiles, indicating continuous spread in Poland.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
P. Urbanowicz, R. Izdebski, A. Baraniak, D. Zabicka, W. Hryniewicz, M. Gniadkowski
Summary: This study identified the molecular and genomic background of the rapid increase of MPPA in Poland, with multi-clonal spread as the leading factor. Various novel and specific characteristics of MPPA were also revealed through the analysis.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Microbiology
Monika Lachowska, Radoslaw Izdebski, Pawel Urbanowicz, Dorota Zabicka, Barbara Krolak-Olejnik
Summary: This study reported a fatal C. sakazakii ST1 infection in a premature infant without identified bacterial contamination sources following epidemiological investigation. The isolate belonged to the main cluster of international ST1 organisms, showing strong pathogenic potential with presence of IncFIB-like molecule and ESBL phenotype associated with IncX3 plasmid-carried virulence related genes.
Article
Biotechnology & Applied Microbiology
Marta Ilona Wojtys, Radoslaw Jazwiec, Sasa Kazazic, Ivana Lescic Asler, Petar Knezevic, Verica Aleksic Sabo, Marija Luic, Elzbieta Katarzyna Jagusztyn-Krynicka, Agnieszka Bzowska
Summary: The article highlights the importance of designing new drugs to combat Helicobacter pylori strains resistant to antibiotics. The authors identified potential new targets and addressed the issue of poor drug penetration in H. pylori cells. The newly developed cell penetration assessment method is expected to facilitate further drug development and design.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Infectious Diseases
R. Izdebski, M. Biedrzycka, P. Urbanowicz, W. Papierowska-Kozdoj, M. Dominiak, D. Zabicka, M. Gniadkowski
Summary: The increase in NDM-1-positive E. hormaechei cases in Poland is a result of uncontrolled spread of NDM-1-producing K. pneumoniae genotypes. Different E. hormaechei lineages have acquired NDM-encoding plasmids in various locales, leading to consecutive outbreaks.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Marta Narczyk, Marta Ilona Wojtys, Ivana Lescic Asler, Biserka Zinic, Marija Luic, Elzbieta Katarzyna Jagusztyn-Krynicka, Zoran Stefanic, Agnieszka Bzowska
Summary: Helicobacter pylori is a global health threat, infecting around 50% of the world's population. This study suggests that purine nucleoside phosphorylase (PNP) could be a new drug target for H. pylori eradication. The inhibition constants of PNP inhibitors were in the micromolar range, with 6-benzylthio-2-chloropurine having the lowest constant. X-ray structures of PNP-inhibitor complexes provided insights into possible tighter binding structures.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2022)
Article
Infectious Diseases
Pawel Urbanowicz, Radoslaw Izdebski, Marta Biedrzycka, Elzbieta Literacka, Waleria Hryniewicz, Marek Gniadkowski
Summary: This study provides a comprehensive analysis of MBL-producing P. putida group isolates from Poland, revealing high diversity and the presence of three international lineages, each internally diversified by MBL-encoding structures.
INFECTIOUS DISEASES AND THERAPY
(2022)
Article
Infectious Diseases
M. Biedrzycka, R. Izdebski, P. Urbanowicz, M. Polanska, W. Hryniewicz, M. Gniadkowski, E. Literacka
Summary: A new multidrug-resistant lineage of ST23 Klebsiella pneumoniae has emerged in Europe, posing a potential threat to public health. Some isolates have multiple virulence loci, while others are more abundant in plasmids.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
M. Biedrzycka, P. Urbanowicz, D. Zabicka, W. Hryniewicz, M. Gniadkowski, R. Izdebski
Summary: This study elucidates the role of the Klebsiella oxytoca species complex (KoSC) in the epidemiology of VIM-type MBL-producing Enterobacterales in Poland. K. oxytoca was found to be the predominant species, and clonal expansion of the K. oxytoca-In237-like lineage was identified as the main driver for the high prevalence of VIM producers. The enhanced epidemic potential of this organism poses a danger on an international scale.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)