Article
Biochemistry & Molecular Biology
Heather Coatsworth, Jovana Bozic, Juliana Carrillo, Eva A. Buckner, Adam R. Rivers, Rhoel R. Dinglasan, Derrick K. Mathias
Summary: Virome studies have shown the presence of RNA viruses in animals, leading to a rethinking of the relationships between organisms and their microbiota. Mosquito viromes, especially those of Aedes aegypti, could potentially contain a core virome that impacts the basic biology of the host. This study investigated the virome composition in A. aegypti and found varying compositions across samples, suggesting the potential for a vertically maintained core virome.
Article
Public, Environmental & Occupational Health
Jurgen P. Moonen, Michelle Schinkel, Tom van der Most, Pascal Miesen, Ronald P. van Rij
Summary: Mosquitoes are important vectors for infectious viral diseases, and understanding the mosquito virome is crucial for studying disease emergence and outbreak dynamics. A searchable database of insect-specific viruses associated with mosquitoes has been constructed, providing valuable information on their global distribution and the challenges of integrating virome data.
Article
Multidisciplinary Sciences
Judith U. Oguzie, Udoka C. Nwangwu, Paul E. Oluniyi, Testimony J. Olumade, Uwem E. George, Akano Kazeem, Bolajoko E. Bankole, Farida O. Brimmo, Chukwuemeka C. Asadu, Okechukwu C. Chukwuekezie, Josephine C. Ochu, Catherine O. Makwe, Festus A. Dogunro, Cosmas O. Onwude, William E. Nwachukwu, Ebuka K. Ezihe, Gilkenny K. Okonkwo, Ndubuisi E. Umazi, Jacob Maikere, Nneka O. Agashi, Emelda I. Eloy, Stephen O. Anokwu, Angela I. Okoronkwo, Ebuka M. Nwosu, Sandra O. Etiki, Ifeoma M. Ngwu, Chikwe Ihekweazu, Onikepe A. Folarin, Isaac O. O. Komolafe, Christian T. Happi
Summary: This study used metagenomic sequencing to identify viruses in mosquito vectors during Yellow fever outbreaks in Nigeria. Multiple viruses belonging to different families were detected, providing insights into the virome of mosquito vectors in Nigeria. Although the pathogenicity of these viruses is currently unknown, they are closely related to known arboviruses.
SCIENTIFIC REPORTS
(2022)
Article
Entomology
Tai-Chih Kuo, Chien-Chung Lin, Ching-Chu Tsai, Shiang-Jiuun Chen, Tso-Min Hung, Che-Chu Hsieh, Ja-Yu Lu, Rong-Nan Huang
Summary: The study investigated the toxicity of terahertz-based functional mineral water to mosquito larvae, pupae, and larvivorous copepods, showing concentration-dependent effects and potential as a biodegradable eco-friendly bioinsecticide. Terahertz-based functional mineral water decreased mosquito hatching and had toxic effects on larvae and pupae, particularly on Aedes albopictus and Cx. quinquefasciatus. The larvicidal and pupicidal activity of the water decreased after 24 hours, suggesting its potential use for mosquito control.
Article
Virology
Alexandre Freitas da Silva, Filipe Zimmer Dezordi, Lais Ceschini Machado, Rodrigo Dias de Oliveira, Si Qin, Hang Fan, Xianglilan Zhang, Yigang Tong, Monica Medeiros Silva, Elgion Lucio Silva Loreto, Gabriel Luz Wallau
Summary: This study characterized the RNA viruses interacting with Mansonia wilsoni and Coquillettidia hermanoi mosquito species, identifying new viral lineages and enriching our understanding of the virome of these two sylvatic Mansoniini mosquitoes.
Article
Virology
Konstantinos Konstantinidis, Nikolas Dovrolis, Adamantia Kouvela, Katerina Kassela, Maria Goreti Rosa Freitas, Andreas Nearchou, Michael de Courcy Williams, Stavroula Veletza, Ioannis Karakasiliotis
Summary: Mosquitoes are key vectors for emerging infectious diseases, and recent research has shown that they carry a diverse array of viruses that may impact their competence as vectors. A study of the core virome in a local ecosystem with 24 mosquito species identified multiple viruses, including novel ones, and revealed complex relationships among mosquito species and virus families. Analysis of mixed-species mosquito pools showed significant overlap with individual species viromes, indicating a stable core virome in the most abundant mosquito species in the ecosystem. The study emphasizes the importance of a holistic approach to understanding mosquito viromes in diverse ecosystems.
Article
Entomology
Marina E. O. Rangel, Luana P. R. Oliveira, Aline D. Cabral, Katharyna C. Gois, Marcos V. M. Lima, Beatriz C. A. A. Reis, Fernando L. A. Fonseca, Marcia A. Speranca, Flavia S. Gehrke, Gabriel Z. Laporta
Summary: The study screened Aedes mosquitoes in an urban area in southeastern Brazil and detected DENV-2 RNA in Aedes albopictus and Guadeloupe mosquito virus RNA in Aedes aegypti. The findings suggest that DENV-2 may have high infection rates and be endemic in the urban areas of Santo Andre, Brazil.
Article
Biology
Katherine Heath, Michael B. Bonsall, Jerome Marie, Herve C. Bossin
Summary: This study used a combination of field data and remote sensing data to model the environmental heterogeneity and its effects on the distribution of Ae. polynesiensis. The study found that the dispersal behavior of the mosquitoes and changes in environmental suitability had complex spatial effects on their distribution.
MATHEMATICAL BIOSCIENCES
(2022)
Article
Public, Environmental & Occupational Health
Qin Wu, Cheng Guo, Xiao-kang Li, Bo-Yang Yi, Qian-Lin Li, Zhong-Min Guo, Jia-Hai Lu
Summary: Mosquitoes are important reservoirs of viruses and vectors of zoonotic pathogens. Blood-fed mosquitoes have been used to determine infection status, and surveillance at the human-animal-environment interface is crucial for early detection of emerging infectious diseases. In this study, mosquitoes from seven habitats in Guangdong Province, China were sampled to analyze viral communities and blood feeding patterns. A total of 101 putative viruses were identified, with Culex mosquitoes showing richer viral diversity than Aedes mosquitoes. Blood meal analysis revealed different host sources, including humans, domestic animals, and wildlife. The findings highlight the risk of cross-species transmission at the human-animal-environment interface.
Article
Multidisciplinary Sciences
Kavita Lole, Ashwini Ramdasi, Sucheta Patil, Shivani Thakar, Amol Nath, Onkar Ghuge, Abhranil Gangopadhayya, Anakkathil B. Sudeep, Sarah Cherian
Summary: This study found that Phasi Charoen-like virus (PCLV) is predominant in mosquitoes in India, and its genetic homogeneity suggests less variability compared to other viruses. The study also observed the transmission of PCLV during the reproductive process of mosquitoes. These findings are important for understanding the transmission and evolution of viruses carried by mosquitoes.
Article
Environmental Sciences
K. Lamy, A. Tran, T. Portafaix, M. D. Leroux, T. Baldet
Summary: This study investigates the impact of climate change on Ae. albopictus abundance and finds that temperature and precipitation significantly influence its abundance. Decreasing precipitation in low-elevation areas leads to a reduction in Ae. albopictus abundance, while in mid and high-elevation areas, the decrease in precipitation is counterbalanced by significant warming, resulting in increased abundance.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Mathematical & Computational Biology
Mengyun Zhang, Zhigui Lin, Huaiping Zhu
Summary: This paper discusses the dengue virus transmitted by Aedes aegypti mosquitoes and establishes a model to investigate the population stability of mosquitoes and the conditions for dengue virus transmission.
INTERNATIONAL JOURNAL OF BIOMATHEMATICS
(2021)
Article
Microbiology
Maria Vittoria Mancini, Rapeepat Tandavanitj, Thomas H. H. Ant, Shivan M. M. Murdochy, Daniel D. D. Gingell, Chayanee Setthapramote, Piyatida Natsrita, Alain Kohl, Steven P. P. Sinkins, Arvind H. H. Patel, Giuditta De Lorenzo
Summary: In this study, a promising Zika virus vaccine candidate was validated in a mosquito-mouse transmission model, showing its ability to protect animals from the disease and reduce virus transmission. The study also revealed the enhancement of viral infection and spread through mosquito bites.
Article
Microbiology
Matheus A. Duarte, Fabricio S. Campos, Osvaldo F. Araujo Neto, Leonardo A. Silva, Arthur B. Silva, Thalita C. Aguiar, Raissa N. Santos, Ueric J. B. Souza, Giselly B. Alves, Fernando L. Melo, Daniel M. P. Ardisson-Araujo, Raimundo W. S. Aguiar, Bergmann M. Ribeiro
Summary: This study used high-throughput sequencing to characterize virus sequences in wild-caught Ae.aegypti mosquitoes in Tocantins state, Brazil, discovering new viral strains and contributing to the understanding of virus diversity in mosquitoes.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2022)
Article
Infectious Diseases
Deepa Dharmamuthuraja, P. D. Rohini, M. Iswarya Lakshmi, Kavita Isvaran, Susanta Kumar Ghosh, Farah Ishtiaq
Summary: This study in an Indian city found that landscape ecology plays a role in the diversity and abundance of mosquitoes, particularly in man-made containers. The findings suggest that mosquito surveillance and dengue transmission reduction programs should focus on urban environments and neighborhood monitoring.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Environmental Sciences
Lu Zhao, Evans Atoni, Raphael Nyaruaba, Yao Du, Huaiyu Zhang, Oscar Donde, Doudou Huang, Shuqi Xiao, Nanjie Ren, Teng Ma, Zhu Shu, Zhiming Yuan, Lei Tong, Han Xia
Summary: Wastewater-based epidemiology (WBE) is an effective environmental surveillance tool for monitoring fecal-oral pathogen infections within a community. This study focused on detecting SARS-CoV-2 RNA in various samples from wastewater systems and related environments in Wuhan during the COVID-19 middle and low risk periods. The findings demonstrated the significance of WBE in continuous surveillance of SARS-CoV-2, even when the COVID-19 prevalence is low.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2022)
Article
Virology
Yi Huang, Hongqing Zhang, Xiaodan Li, Lu Zhao, Dirui Cai, Shunlong Wang, Nanjie Ren, Haixia Ma, Doudou Huang, Fei Wang, Zhiming Yuan, Bo Zhang, Han Xia
Summary: This study detected a strain of mosquito flavivirus (MFV) in Culicoides collected from Yunnan, China, and successfully rescued the virus using an infectious clone. Further analysis of the biological features of the virus in vitro and in vivo showed strong replication ability in cells but low infection rate in mosquitoes.
Article
Infectious Diseases
Cihan Yang, Fei Wang, Doudou Huang, Haixia Ma, Lu Zhao, Guilin Zhang, Hailong Li, Qian Han, Dennis Bente, Ferdinand Villanueva Salazar, Zhiming Yuan, Han Xiai
Summary: The global impact of mosquito-borne diseases has increased in recent decades. This study found that Aedes aegypti mosquitoes have the potential to spread Ebinur Lake virus (EBIV) through biting, with a transmission rate of up to 11.8%. The study also revealed a complex interplay between EBIV and the mosquito's immune system, which could affect its vector competence.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Letter
Cell Biology
Dali Tong, Mei Zhang, Yunru Yang, Han Xia, Haiyang Tong, Huajun Zhang, Weihong Zeng, Muziying Liu, Yan Wu, Huan Ma, Xue Hu, Weiyong Liu, Yuan Cai, Yanfeng Yao, Yichuan Yao, Kunpeng Liu, Shifang Shan, Yajuan Li, Ge Gao, Weiwei Guo, Yun Peng, Shaohong Chen, Juhong Rao, Jiaxuan Zhao, Juan Min, Qingjun Zhu, Yanmin Zheng, Lianxin Liu, Chao Shan, Kai Zhong, Zilong Qiu, Tengchuan Jin, Sandra Chiu, Zhiming Yuan, Tian Xue
Article
Infectious Diseases
Yanshuang Wang, Xuemiao Li, David A B Dance, Han Xia, Chen Chen, Nini Luo, Anyang Li, Yanmei Li, Qiao Zhu, Qinghui Sun, Xingyong Wu, Yingfei Zeng, Lin Chen, Shen Tian, Qianfeng Xia
Summary: The study evaluated the clinical potential of Burkholderia pseudomallei phages isolated from Hainan, China, for treating melioidosis. Results showed that the phage exhibited promising lytic properties and stability, significantly improving cell and nematode survival rates.
INFECTIOUS DISEASES OF POVERTY
(2022)
Article
Multidisciplinary Sciences
Fei Wang, Cihan Yang, Shunlong Wang, Qun Wu, Christabel Ochieng, Zhiming Yuan, Han Xia
Summary: Mosquito-borne viruses pose a severe threat to public health as they are transmitted by many mosquito species. This study presents a method for detecting viruses in the salivary glands of Aedes aegypti, which can help assess the risk of these viruses spread by mosquitoes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Pharmacology & Pharmacy
Nanjie Ren, Fei Wang, Lu Zhao, Shunlong Wang, Guilin Zhang, Jiaqi Li, Bo Zhang, Jinglin Wang, Eric Bergeron, Zhiming Yuan, Han Xia
Summary: Orthobunyaviruses can cause severe diseases and pose a potential threat to human health and the economy. This study focuses on Ebinur lake virus (EBIV), a newly classified orthobunyavirus, and investigates its replication, pathogenesis, and potential therapies by using a reverse genetics system. The results show that ribavirin and Tyrphostin A9 have inhibitory effects against EBIV.
ANTIVIRAL RESEARCH
(2022)
Article
Cell Biology
Jie Yao, Shuming Tang, Chenyan Shi, Yunzhi Lin, Lanlan Ge, Qinghua Chen, Baoru Ou, Dongyu Liu, Yuyang Miao, Qiujie Xie, Xudong Tang, Jia Fei, Guangyi Yang, Jun Tian, Xiaobin Zeng
Summary: IsoGinkgetin (ISO) inhibits the proliferation and migration of hepatocellular carcinoma (HCC) cells through multiple mechanisms, including inducing autophagy, suppressing SLC2A1/GLUT1 expression and glucose uptake, and activating the AMPK-ULK1 pathway. These findings provide new insights into the molecular mechanisms of HCC treatment.
Article
Infectious Diseases
Jinxin Meng, Fei Wang, Yuwen He, Nan Li, Zhenxing Yang, Jun Yao, Shunlong Wang, Guodian Xiong, Zhiming Yuan, Han Xia, Jinglin Wang
Summary: A novel Oya virus (OYAV) isolate SZC50 was sequenced from midge samples and its host tropism was tested in vitro, showing high susceptibility in pig-derived cell lines. High prevalence of OYAV SZC50 neutralization antibody was found in Yunnan pig serum samples. Pathogenicity of OYAV SZC50 was demonstrated in animal models, with adult and suckling mice being extremely susceptible and chicken embryos showing a fatality rate of 37.5%.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Multidisciplinary Sciences
Ying Huang, Shunlong Wang, Hong Liu, Evans Atoni, Fei Wang, Wei Chen, Zhaolin Li, Sergio Rodriguez, Zhiming Yuan, Zhaoyan Ming, Han Xia
Summary: This study created a comprehensive dataset of arboviruses and ASVs, revealing the diversity, distribution, and biosafety recommendations of these viruses globally. It provides insights into potential interactions, evolution, and risks associated with arboviruses and ASVs.
Article
Biochemistry & Molecular Biology
Guoguo Ye, Zhixiang Xu, Minghui Yang, Jun Wang, Jinhu Liang, Juzhen Yin, Yang Yang, Han Xia, Yingxia Liu
Summary: Based on the surveillance of dengue virus (DENV) infections in Shenzhen, it was found that the majority of DENV-infected patients are young to middle-aged males, and the disease is accompanied by abnormal changes in blood cell percentages. The concentration of these patients in Futian District may be due to higher mosquito density and temperature. Mosquito infection experiments confirmed the effect of temperature on DENV proliferation and transmission, with constant temperatures enhancing the spread of the virus and increasing the risk of epidemics.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Biochemical Research Methods
Zhaoyan Ming, Xiangjun Chen, Shunlong Wang, Hong Liu, Zhiming Yuan, Minghui Wu, Han Xia
Summary: The article presents HostNet, a deep learning framework for predicting virus hosts from genomic sequences. HostNet utilizes a Transformer-CNN-BiGRU architecture and two enhanced sequence representation modules to overcome the challenges of data deficiency and imbalance. The results show that HostNet outperforms the state-of-the-art deep learning-based method in host prediction accuracy and F1 score. The improved sequence representation modules significantly enhance HostNet's training generalization, performance in challenging classes, and stability.
BMC BIOINFORMATICS
(2023)
Letter
Virology
Mengying Liang, Wanbo Tai, Yunyun Wang, Yulong Dai, Yanfeng Yao, Juan Min, Yiwu Zhou, Liang Liu, Chao Shan, Han Xia, Zhiming Yuan
Letter
Virology
Ge Gao, Xue Hu, Yiwu Zhou, Juhong Rao, Xiaoyu Zhang, Yun Peng, Jiaxuan Zhao, Yanfeng Yao, Kunpeng Liu, Mengying Liang, Hang Liu, Fei Deng, Han Xia, Chao Shan, Zhiming Yuan