Article
Medicine, General & Internal
Rebecca Panconesi, Mauricio Flores Carvalho, Janina Eden, Marilena Fazi, Fariha Ansari, Leandro Mancina, Nadia Navari, Richard Xavier Sousa Da Silva, Daniele Dondossola, Lucia Bautista Borrego, Matthias Pietzke, Adriano Peris, David Meierhofer, Paolo Muiesan, Alexander Galkin, Fabio Marra, Philipp Dutkowski, Andrea Schlegel
Summary: This study investigated the effects of NRP and HOPE on DCD liver transplantation using an animal model. The results showed that HOPE can reduce mitochondrial injury and inflammation, and improve post-transplant survival.
Article
Gastroenterology & Hepatology
Andrea Schlegel, Matteo Mueller, Xavier Mueller, Janina Eden, Rebecca Panconesi, Stefanie von Felten, Klaus Steigmiller, Richard X. Sousa Da Silva, Olivier de Rougemont, Jean-Yves Mabrut, Mickael Lesurtel, Miriam Cortes Cerisuelo, Nigel D. Heaton, Marc Antoine Allard, Rene Adam, Diethard Monbaliu, Ina Jochmans, Martijn P. D. Haring, Robert J. Porte, Alessandro Parente, Paolo Muiesan, Philipp Kron, Magdy Attia, Dagmar Kollmann, Gabriela Berlakovich, Xavier Rogiers, Karin Petterson, Anne L. Kranich, Stefanie Amberg, Beat Mullhaupt, Pierre-Alain Clavien, Philipp Dutkowski
Summary: Hypothermic oxygenated perfusion (HOPE) after cold storage of donated livers reduces the incidence of complications and graft failure in liver transplantation.
JOURNAL OF HEPATOLOGY
(2023)
Article
Medicine, General & Internal
Fabian Horne, Moritz Drefs, Malte Joachim Schirren, Dominik Thomas Koch, Ganildo Cepele, Severin Johannes Jacobi, Elnaz Payani, Nikolaus Boerner, Jens Werner, Markus Otto Guba, Dionysios Koliogiannis
Summary: The study demonstrates that pre-transplant graft preconditioning with HOPE can reduce the incidence of PRS and EAD in liver transplantation, as well as improve hemodynamic stability. HOPE can also mitigate ES and prevent hyperpotassemic complications.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Niels Moeslund, Imran A. Ertugrul, Michiel A. Hu, Frederik Flyvholm Dalsgaard, Lars Bo Ilkjaer, Pia Ryhammer, Michael Pedersen, Michiel E. Erasmus, Hans Eiskjaer
Summary: In this study, a novel oxygenated hypothermic machine perfusion (HMP) system was compared to static cold storage (SCS) in heart transplantation (HTX). The results showed that hearts preserved with HMP exhibited increased biventricular contractility post-HTX and required less dobutamine for maintaining cardiac output. Additionally, HMP groups had lower levels of myocardial damage. Therefore, DCD HTX can be successfully performed using DPP followed by preservation with HMP.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Article
Engineering, Biomedical
Amanda P. C. S. Boteon, Marisa R. D. Lima, Bianca Della Guardia, Mauricio F. Carvalho, Andrea Schlegel, Yuri L. Boteon
Summary: This case report highlights the use of HOPE treatment for an extended criteria donor organ, allowing for successful liver retransplantation in a complex surgical case. Further research is needed, but HOPE may have the potential to expand the donor organ pool and reduce the time constraints of transplantation surgeries.
Review
Medicine, General & Internal
Fabio Melandro, Riccardo De Carlis, Francesco Torri, Andrea Lauterio, Paolo De Simone, Luciano De Carlis, Davide Ghinolfi
Summary: This review summarizes the current literature on viability criteria during normothermic and hypothermic machine perfusion, and discusses the future steps and evolution of these technologies.
MEDICINA-LITHUANIA
(2022)
Article
Medicine, General & Internal
R. Van Rijn, I. J. Schurink, Y. de Vries, A. P. van den Berg, M. Cortes Cerisuelo, S. Darwish Murad, J. I. Erdmann, N. Gilbo, R. J. de Haas, N. Heaton, B. van Hoek, V. A. L. Huurman, I. Jochmans, O. B. van Leeuwen, V. E. de Meijer, D. Monbaliu, W. G. Polak, J. J. G. Slangen, R. I. Troisi, A. Vanlander, J. de Jonge, R. J. Porte
Summary: This study demonstrated that hypothermic oxygenated machine perfusion can reduce the risk of nonanastomotic biliary strictures following liver transplantation from donors after circulatory death, with lower rates of complications compared to conventional static cold storage.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Biology
Francesco Vasuri, Giuliana Germinario, Carmen Ciavarella, Michele Carroli, Ilenia Motta, Sabrina Valente, Matteo Cescon, Antonia D'Errico, Gianandrea Pasquinelli, Matteo Ravaioli
Summary: Little is known about the functions and mechanisms of endothelial cells in liver grafts, especially the effects of machine perfusion techniques. In this study, different endothelial markers were analyzed in liver grafts using immunohistochemistry and gene expression analysis. The results showed severe depression of endothelial trophism in liver grafts, which was restored after reperfusion. The execution of hypothermic oxygenated perfusion seemed to improve this recovery. These findings may contribute to improving knowledge on graft tissues and customizing perfusion techniques to improve liver transplant outcomes.
Article
Surgery
Michal Grat, Marcin Morawski, Andriy Zhylko, Pawel Rykowski, Maciej Krasnodebski, Anya Wyporski, Jan Borkowski, Zbigniew Lewandowski, Konrad Kobryn, Rafal Stankiewicz, Jan Stypulkowski, Waclaw Holowko, Waldemar Patkowski, Magdalena Mielczarek-Puta, Marta Struga, Benedykt Szczepankiewicz, Barbara Gornicka, Marek Krawczyk
Summary: This study suggests that routine use of end-ischemic hypothermic oxygenated machine perfusion (HOPE) in liver transplantations from donors after brain death (DBD) is not justified, as the clinical benefits are limited to high-risk donors.
Article
Immunology
Riccardo De Carlis, Andrea Schlegel, Samuele Frassoni, Tiziana Olivieri, Matteo Ravaioli, Stefania Camagni, Damiano Patrono, Domenico Bassi, Duilio Pagano, Stefano Di Sandro, Andrea Lauterio, Vincenzo Bagnardi, Salvatore Gruttadauria, Umberto Cillo, Renato Romagnoli, Michele Colledan, Matteo Cescon, Fabrizio Di Benedetto, Paolo Muiesan, Luciano De Carlis
Summary: This study found that NRP and D-HOPE yield good results in DCD livers with prolonged warm ischemia in Italy, reducing the incidence of kidney injury.
Article
Medicine, General & Internal
Janina Eden, Eva Breuer, Dominique Birrer, Matteo Mueller, Matthias Pfister, Hemma Mayr, Keyue Sun, Jeannette Widmer, Florian Huwyler, Udo Ungethuem, Bostjan Humar, Anurag Gupta, Stefanie Schiess, Martin Wendt, Franz Immer, Andreas Elmer, David Meierhofer, Andrea Schlegel, Philipp Dutkowski
Summary: This study reports on the concept of liver assessment during ex situ hypothermic oxygenated perfusion (HOPE) and emphasizes its significant impact on liver utilization. The findings show that HOPE treatment combined with viability assessment is highly suitable for evaluating liver grafts, enabling surgeons to make informed decisions on whether to proceed with the transplant.
Article
Surgery
Lorenzo Maroni, Nicola Musa, Matteo Ravaioli, Daniele Eliseo Dondossola, Giuliana Germinario, Laurent Sulpice, Matteo Cescon, Giorgio Ettore Rossi, Karim Boudjema
Summary: Italian and French groups had similar post-LT outcomes, probably related to the use of HOPE after CS in the case of long WIT.
CLINICAL TRANSPLANTATION
(2021)
Article
Biochemistry & Molecular Biology
Caterina Lonati, Andrea Schlegel, Michele Battistin, Riccardo Merighi, Margherita Carbonaro, Paola Dongiovanni, Patrizia Leonardi, Alberto Zanella, Daniele Dondossola
Summary: The study investigated how analysis of effluent fluids collected during organ flush can improve selection criteria for HOPE utilization. The results showed that livers assigned to HOPE had different molecular profiles compared to grafts transplanted without this procedure, with changes in inflammatory mediators and cytokines. The study demonstrates that effluent molecular analysis can provide directions about the use of HOPE.
Article
Surgery
Rahel K. Wyss, Natalia Mendez Carmona, Maria Arnold, Adrian Segiser, Matteo Mueller, Philipp Dutkowski, Thierry P. Carrel, Sarah L. Longnus
Summary: The study investigated a new method for heart transplants used in DCD, called HOPE, which provides improved cardioprotection by reducing cardiac succinate levels, alleviating graft injury.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Review
Medicine, General & Internal
Joseph Mugaanyi, Lei Dai, Changjiang Lu, Shuqi Mao, Jing Huang, Caide Lu
Summary: Machine perfusion, particularly HOPE/D-HOPE and NMP, shows promise as alternatives to static cold storage for the preservation of donor livers. These methods improve graft and patient survival rates and reduce the incidence of biliary complications. They have the potential to address the gap between demand and availability of donor livers by enabling the utilization of marginal livers.
JOURNAL OF CLINICAL MEDICINE
(2023)