Article
Biochemistry & Molecular Biology
Dmitry Deryabin, Kseniya Inchagova, Elena Rusakova, Galimzhan Duskaev
Summary: The study aimed to find the most effective combinations of coumarins and small plant-derived molecules to inhibit quorum sensing (QS) in Chromobacterium violaceum ATCC 31532. Testing two-component compositions showed that 7,8-dihydroxy-4-methylcoumarin, 4-hexyl-1,3-benzendiol, and gamma-octalactone showed a supra-additive anti-QS effect. Combinations of all three molecules resulted in a three- to five-fold reduction in the concentration of each compound needed to achieve EC50 against QS in C. violaceum ATCC 31532.
Article
Biochemistry & Molecular Biology
Zijie Zhang, Lei Zhang, Yang Liu, Xulan Shang, Shengzuo Fang
Summary: R2R3-MYB genes play an important role in plant growth and response to environmental stress. This study identified and analyzed the function of R2R3-MYB genes in Cyclocarya paliurus in response to salt stress. The results showed that the R2R3-MYB genes of C. paliurus have conserved functional domains, and four genes that might be involved in salt stress response were identified.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Tingting Li, Jishi Wang, Zhiying Wang, Lu Qiao, Rui Liu, Shanshan Li, Ailiang Chen
Summary: Mitochondrial genes are commonly used for PCR-based meat adulteration authentication, but variations in gene copies can lead to quantification errors. This study developed a quantitative method using single-copy nuclear genes to accurately identify mutton adulteration, achieving high recovery rates for simulated mutton-pork, mutton-chicken, and mutton-duck samples.
Article
Biochemistry & Molecular Biology
Peter Braun, Martin Duy-Thanh Nguyen, Mathias C. Walter, Gregor Grass
Summary: A real-time PCR assay targeting a specific single nucleotide polymorphism in Bacillus anthracis genomes was validated, showing high specificity, linearity, and sensitivity. Adapting the assay for reverse transcription PCR on 16S rRNA transcripts significantly increased sensitivity compared to DNA-targeting assays, offering great potential for molecular diagnostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Martin Paolo Soto-Aceves, Miguel Cocotl-Yanez, Luis Servin-Gonzalez, Gloria Soberon-Chavez
Summary: This study reveals that under phosphate limitation, even in the PAO1 type strain, the absence of LasR has no effect on the production of virulence factors and gene expression profile, whereas in a condition of phosphate repletion, the LasR hierarchy is maintained.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Horticulture
Chenjie Zhang, Chengwei Song, Linfeng Chen, Huili Ma, Yabing Zhang, Dalong Guo, Lili Guo, Xiaogai Hou
Summary: The aim of this study was to identify suitable reference genes for normalizing the expression levels of miRNA in different developmental stages and tissues. Five algorithms were used for stability analysis, and the results identified mtr-MIR160b-p3 and gma-miR394a-5p as the most stable miRNAs during bud development in early-flowering tree peony 'Feng dan', while PC-5p-19095 was the most stable during bud development in late-flowering tree peony 'Lian he'. Additionally, mtr-miR159a was found to be the most stable miRNA during flower development stages of different tree peony varieties.
Article
Biotechnology & Applied Microbiology
Jeongah Song, Jeonghee Cho, Jeongsik Park, Jeong Ho Hwang
Summary: This study identified stable reference genes in seven tissues across four developmental stages in minipigs, which can be used to evaluate mRNA expression levels. HPRT1 and 18S were found to be the most stable genes. The expression levels of ACE2 in the intestine and kidney increased significantly at PND28, consistent with the ACE2 expression pattern in humans.
Article
Ecology
Agnes Vallier, Elisa Dell'Aglio, Mariana Galvao Ferrarini, Ophelie Hurtado, Carole Vincent-Monegat, Abdelaziz Heddi, Rita Rebollo, Anna Zaidman-Remy
Summary: This study used transcriptomic high-throughput analysis to identify a subset of stably expressed genes from different developmental stages of the gut and associated-bacteriomes of Sitophilus oryzae, which can be used as reference genes in qRT-PCR experiments. Two genes, TAO and YTH3, were selected as suitable genes for comparing transcript abundance in symbiotic and aposymbiotic insects at various developmental stages. These well-conserved genes have the potential to be applied in other insect species, emphasizing the importance of using unbiased methods for reference gene selection.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2023)
Article
Multidisciplinary Sciences
Xiujie Yin, Taotao He, Kun Yi, Yihang Zhao, Yao Hu, Jiaxue Liu, Xiaomeng Zhang, Lingdong Meng, Lina Wang, Haoyue Liu, Yonggang Li, Guowen Cui
Summary: Twelve reference genes were selected based on transcriptomic data for stable expression analysis in various organs of Caucasian clover under different stress conditions. Results showed that different reference genes were most stable under different stress conditions, with RCD1 and NLI2 identified as the most stable reference genes across all organs.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Peng Guan, Xiaojie Wang, Zijie Dong, Miao Song, Hongshuai Zhu, Biao Suo
Summary: This study evaluated the inhibitory effect of cinnamaldehyde on Listeria monocytogenes at 4 degrees C and investigated the mechanism behind this inhibition. The combination of cinnamaldehyde and low temperature treatment disrupted the gene regulation and caused severe damage to the cell envelope of L. monocytogenes. It was found that cinnamaldehyde interacted with specific proteins, leading to interference with regulator activity. These findings provide new insights for the safety control of chilled meat.
Review
Microbiology
Mei-Chen Zhu, Xue-Mei Li, Na Zhao, Le Yang, Ke-Qin Zhang, Jin-Kui Yang
Summary: This review summarizes the potential regulatory mechanism of trap formation in nematode-trapping fungi, including signaling pathways, small molecule compounds, and the involvement of organelles and receptors, and highlights the research focus for the future.
Article
Plant Sciences
Rohit Bharati, Madhab Kumar Sen, Ram Kumar, Aayushi Gupta, Jana Ziarovska, Eloy Fernandez-Cusimamani, Olga Leuner
Summary: This study assessed the suitability of ten commonly used reference genes as optimal reference genes in M. officinalis under different abiotic stress conditions and plant organs. The combination of EF-1 alpha and GAPDH was recommended as the best reference genes for gene expression studies in M. officinalis.
Article
Oncology
Merve Bilgin, Sibel Dosler, Gulten Otuk
Summary: This study investigated the effects of quorum-sensing molecules on mature Staphylococcal biofilms and found that they enhanced the activity of antibiotics, suggesting a new strategy for biofilm control.
JOURNAL OF CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Kathannan Sankar, Hyung Joo Yoon, Young Bo Lee, Kyeong Yong Lee
Summary: Bumble bees are important alternative pollinators and model insects. This study identified stable reference genes for the bumble bee species and their different lines and tissues using RT-qPCR procedures and four analysis programs. The results emphasized the need to evaluate the stability of candidate reference genes for any differently designed lines and tissue conditions in bumble bee species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Eiseul Kim, Hyeon-Be Kim, Seung-Min Yang, Dayoung Kim, Hae-Yeong Kim
Summary: Identification of specific genes for the Lactobacillus plantarum group through comparative genomics led to the development of an accurate real-time PCR assay for detection. The specificity of primers was confirmed through evaluation on lactic acid bacteria, and the developed assay successfully distinguished and quantified L. plantarum group species or subspecies in probiotic products and fermented food samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Shaymaa Hassan Abdel-Rhman, Areej Mostafa El-Mahdy, Mohammed El-Mowafy
BIOMED RESEARCH INTERNATIONAL
(2015)
Article
Chemistry, Applied
Hatem Gaffer, Hamada Mashaly, Shaymaa Hassan Abdel-Rhman, Mamdouh Hammouda
PIGMENT & RESIN TECHNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Shaymaa H. Abdel-Rhman
ANNALS OF MICROBIOLOGY
(2019)
Article
Microbiology
Soha El-Shaer, Shaymaa Hassan Abdel-Rhman, Rasha Barwa, Ramadan Hassan
JUNDISHAPUR JOURNAL OF MICROBIOLOGY
(2018)
Article
Multidisciplinary Sciences
Shaymaa H. Abdel-Rhman
Article
Infectious Diseases
Shaymaa H. Abdel-Rhman, Dina E. Rizk
Summary: This study evaluated the performance of various PCR-based typing techniques in determining the genetic diversity of Pseudomonas aeruginosa urinary isolates. RAPD primer (P-640) showed the highest discrimination power, while ERIC-PCR excelled in most marker parameters. Combining data from different methods can enhance the performance and predictive power of typing methods.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Shaymaa H. Abdel-Rhman, Rehab M. Elbargisy, Dina E. Rizk
Summary: This study reported a high prevalence of PMQR genes and integrons among clinical E. coli isolates. Although PMQR genes are not cassette-born, they were associated with the presence of integrons, contributing to the widespread of quinolone resistance in Egypt.
INTERNATIONAL JOURNAL OF MICROBIOLOGY
(2021)
