Article
Cell Biology
Na Yuan, Wen Wei, Li Ji, Jiawei Qian, Zhicong Jin, Hong Liu, Li Xu, Lei Li, Chen Zhao, Xueqin Gao, Yulong He, Mingyuan Wang, Longhai Tang, Yixuan Fang, Jianrong Wang
Summary: The bone marrow niche, responsible for maintaining hematopoietic stem cell homeostasis, declines in function with aging and hematological malignancies. This study reveals that disrupting autophagy in HSCs accelerates niche aging, while transplantation of young donor HSCs repairs the niche environment. Further investigation shows that HSCs transdifferentiate into functional niche cells, including mesenchymal stromal cells and endothelial cells, in an autophagy-dependent manner. These findings provide a clinical solution to rejuvenate an aged or damaged bone marrow hematopoietic niche.
Review
Biochemistry & Molecular Biology
Lucas Lange, Michael Morgan, Axel Schambach
Summary: The in vitro generation of hematopoietic cells, especially HSCs, from human pluripotent stem cells has great potential for regenerative medicine, but still faces challenges due to insufficient functionality and quantity of the produced HSCs. Further understanding of the complex processes involved in this conversion is needed to achieve the goal of generating therapeutically applicable PSC-derived HSCs in vitro.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell & Tissue Engineering
Mariko Tsuruda, Saori Morino-Koga, Minetaro Ogawa
Summary: Experimental findings suggest that VE-cad(+) cells from late-stage embryos can differentiate into MCs independently of HSCs in vitro, and have the potential to generate connective tissue MCs in vivo, shedding light on the differentiation mechanism of hematopoietic cells.
Article
Hematology
Cansu Koyunlar, Emanuele Gioacchino, Disha Vadgama, Hans de Looper, Joke Zink, Mariette N. D. ter Borg, Remco Hoogenboezem, Marije Havermans, Mathijs A. Sanders, Eric Bindels, Elaine Dzierzak, Ivo P. Touw, Emma de Pater
Summary: This study demonstrates the crucial role of Gata2 in the regulation of hematopoietic stem cell (HSC) development and function. Gata2 haploinsufficiency affects the transcriptional activation of HSCs and repression of endothelial cell identity, resulting in incomplete maturation of HSCs. Moreover, hematopoietic-specific induction of Gfi1b expression can restore HSC production in gata2b-null zebrafish embryos.
Article
Cell Biology
Yun-Qiao Li, Yandong Gong, Siyuan Hou, Tao Huang, Haizhen Wang, Di Liu, Yanli Ni, Chaojie Wang, Junliang Wang, Jun Hou, Ruichuang Yang, Jing Yan, Guangyu Zhang, Bing Liu, Yu Lan
Summary: This study reveals the spatiotemporal and functional heterogeneity of Hematopoietic stem cells (HSCs) derived from Hemogenic Endothelial Cells (HECs) during embryonic development. PK44 cells in the AGM region and yolk sac display similar developmental dynamics and functional characteristics with endothelial and hematopoietic features. They demonstrate multilineage reconstitution capacity and transcriptional differences, providing a fundamental basis for future research on HSC generation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Hematology
Vincenzo Calvanese, Hanna K. A. Mikkola
Summary: Developmental hematopoiesis is a complex process involving the generation of differentiated blood cells and the establishment of a pool of undifferentiated hematopoietic stem cells (HSCs) for postnatal life. Recent single-cell studies have identified rare human HSCs and traced their origin to a unique type of arterial endothelium in the aorta-gonad-mesonephros region. These studies provide new insights into HSC generation and may inform efforts to generate HSCs from pluripotent stem cells in vitro.
Article
Cell & Tissue Engineering
Emmanuelle Julien, Katia Biasch, Reine El Omar, Jean-Noel Freund, Christian Gachet, Francois Lanza, Manuela Tavian
Summary: The study found that angiotensin-converting enzyme is expressed in hematopoietic progenitors in developing mouse embryos and various elements of the renin-angiotensin system are present in specific regions of human and mouse embryos. Experimentally, angiotensin II signaling is crucial for the generation of hematopoietic cells, with the peptide stimulating hematopoiesis in culture.
Article
Cell & Tissue Engineering
Seon-Hui Shim, Dejene Tufa, Renee Woods, Trahan D. George, Tyler Shank, Ashley Yingst, Jessica Lake, Laura Cobb, Dallas Jones, Kenneth Jones, Michael R. Verneris
Summary: Epigenetic modification plays an important role in hematopoietic cell differentiation. This study demonstrates that histone deacetylase inhibitors can enhance the differentiation of pluripotent stem cells into hematopoietic stem and progenitor cells, overcoming a major bottleneck in cellular immunotherapy.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Review
Cell Biology
Elena Konstantina Siapati, Maria G. Roubelakis, George Vassilopoulos
Summary: The liver has high regenerative capacity, but chronic inflammation and fibrosis caused by various insults can lead to irreversible liver dysfunction. Cell therapy and stem cell transplantation have shown promising results in liver disease treatment, but the underlying mechanisms are still under investigation.
Article
Developmental Biology
Rebecca K. Schiavo, Owen J. Tamplin
Summary: This study reveals the role of Vegfc in the emergence of HSPCs in the zebrafish dorsal aorta and suggests that it regulates HSPC fate by suppressing a myeloid cell fate.
Article
Medicine, Research & Experimental
Hao Wang, Zhenzhen Lin, Zhe Nian, Wei Zhang, Wenxu Liu, Fei Yan, Zengtuan Xiao, Xia Wang, Zhenfa Zhang, Zhenyi Ma, Zhe Liu
Summary: The study identifies GFI1 as a critical regulator of anchorage independence in lung cancer cells, revealing a mechanism by which carcinoma cells hijack a hematopoietic factor for gaining anchorage independence. This could lead to ERK signaling dependency in tumor cells, suggesting potential interventions for suppressing metastasis and improving therapeutic outcomes in cancer.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Multidisciplinary Sciences
Xia Chen, Peiliang Wang, Hui Qiu, Yonglin Zhu, Xingwu Zhang, Yaxuan Zhang, Fuyu Duan, Shuangyuan Ding, Jianying Guo, Yue Huang, Jie Na
Summary: This study provides a comprehensive analysis of the epigenetic landscape of human pluripotent stem cell hematopoietic differentiation and identifies JUNB as an essential transcription factor for hemogenic endothelium development.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Yohko Kitagawa, Akihiro Ikenaka, Ryohichi Sugimura, Akira Niwa, Megumu K. Saito
Summary: Cell differentiation is achieved through the acquisition of a cell type-specific transcriptional program and epigenetic landscape. Research has shown that the patterning of cell type-specific enhancers occurs before cell fate decisions, but the mechanisms for initiating cell specification and properly restricting differentiation remain unclear. In this study, the activation of hematopoietic enhancers during arterialization of hemogenic endothelium, a key step in hematopoiesis, was observed. It was also found that the transcription factor ZEB2, involved in arterial endothelial cell regulation, and the hematopoietic regulator MEIS1 are independently required for activating these enhancers. Deficiency in ZEB2 or MEIS1 impairs hematopoietic cell development. These findings suggest that multiple regulators expressed during early development contribute non-redundantly to the establishment of the hematopoietic enhancer landscape, thereby restricting cell differentiation despite the unrestricted expression of these regulators in hematopoietic cells.
Article
Multidisciplinary Sciences
Leal Oburoglu, Els Mansell, Niels-Bjarne Woods
Summary: Glutamine metabolism plays an essential role in regulating the emergence and specification of hematopoietic cells during endothelial to hematopoietic transition (EHT), with different hematopoietic lineages requiring distinct derivatives of glutamine.
SCIENTIFIC REPORTS
(2021)
Article
Developmental Biology
Laura F. Bennett, Melanie D. Mumau, Yan Li, Nancy A. Speck
Summary: The study reveals that MyD88-dependent toll like receptor (TLR) signaling regulates the numbers of lympho-myeloid biased progenitors (LMPs) and adult-repopulating hematopoietic stem cells (HSCs) in hemogenic endothelial cells and intra-arterial hematopoietic clusters.