Journal
FRONTIERS IN IMMUNOLOGY
Volume 11, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2020.550946
Keywords
perforin-2; mpeg-1; human skin; innate immunity; Staphylococcus epidermidis; gamma delta T cells; cytotoxicity
Categories
Funding
- NIH [NR015649, DK119085]
- Department of Microbiology and Immunology P2 funds
- Wound Healing Society Summer Research Fellowship
Ask authors/readers for more resources
Perforin-2 (P-2) is an antimicrobial protein with unique properties to kill intracellular bacteria. Gamma delta (GD) T cells, as the major T cell population in epithelial tissues, play a central role in protective and pathogenic immune responses in the skin. However, the tissue-specific mechanisms that control the innate immune response and the effector functions of GD T cells, especially the cross-talk with commensal organisms, are not very well understood. We hypothesized that the most prevalent skin commensal microorganism,Staphylococcus epidermidis, may play a role in regulating GD T cell-mediated cutaneous responses. We analyzed antimicrobial protein P-2 expression in human skin at a single cell resolution using an amplified fluorescencein situhybridization approach to detect P-2 mRNA in combination with immunophenotyping. We show thatS. epidermidisactivates GD T cells and upregulates P-2 in human skinex vivoin a cell-specific manner. Furthermore, P-2 upregulation followingS. epidermidisstimulation correlates with increased ability of skin cells to kill intracellularStaphylococcus aureus. Our findings are the first to reveal that skin commensal bacteria induce P-2 expression, which may be utilized beneficially to modulate host innate immune responses and protect from skin infections.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available