Article
Biochemistry & Molecular Biology
Silvia Berto, Martino Marangella, Concetta De Stefano, Demetrio Milea, Pier Giuseppe Daniele
Summary: New complexes such as [Cacit](4-) and [Ca2H2(PO4)(2)] significantly lower beta values in patients with nephrolithiasis. Investigation of the Ca-phosphate-citrate aqueous system through potentiometric titrations revealed that the [Ca(PO4)cit](4-) species is only noticeable at pH > 8.5.
Article
Engineering, Chemical
Holly A. Huellemeier, Necla M. Eren, Joana Ortega-Anaya, Rafael Jimenez-Flores, Dennis R. Heldman
Summary: In food manufacturing, fouling of surfaces affects operational efficiency, with intensity varying based on product formulation and processing temperature. QCM-D can be used to study milk adsorption and fouling, providing a foundation for predicting fouling at a larger scale.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Food Science & Technology
Xinqi Zhao, Cunfang Wang, Ming Cheng, Xiaoning Zhang, Hua Jiang
Summary: This study investigated the changes in micellar casein properties of goat milk following the addition of two types of calcium and decalcification. The results showed that both calcium addition and removal significantly affected the hydration rate, zeta potential, turbidity, and microstructure of casein. This research provides insights into the impact of calcium manipulation on the properties of goat milk casein, which could be valuable for the development of calcium-based functional dairy products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ju-Yang Jung, Ji-Won Kim, Sang-Won Lee, Wook-Young Baek, Hyoun-Ah Kim, Chang-Hee Suh
Summary: This study evaluates the clinical value of Immunoglobulin gamma-3 chain C (IGHG3) by measuring and comparing its levels in different body fluids of patients with systemic lupus erythematosus (SLE). The levels of IGHG3 in saliva, serum, and urine were measured and analyzed in patients with SLE and healthy controls. It was found that IGHG3 levels were increased in the saliva, serum, and urine of patients with SLE, and correlated with certain clinical characteristics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Minjoo Park, Sungjin Park, Ji-Yeon Yoo, Yerin Kim, Kwang Min Lee, Dae-Youn Hwang, Hong-Joo Son
Summary: Biocalcification, a natural biochemical process, has the potential to be a promising method for sequestering heavy metals, healing concrete structures, and stabilizing soil. This study focused on maximizing the production of calcium carbonate by manipulating the medium composition, resulting in a significantly higher yield compared to traditional methods. The alkaliphilic Bacillus psychrodurans LC40 showed promising results in producing calcium carbonate in strongly alkaline conditions, indicating its potential for ecofriendly biosealant development.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Dong Hyuk Jung, Byoungjin Park, Yong Jae Lee
Summary: This study aimed to assess the effects of serum calcium and phosphate levels on incident ischemic heart disease (IHD) in a large-scale community-dwelling Korean cohort. The study found that serum calcium and phosphate levels were positively associated with IHD incidence, while the serum calcium/phosphate ratio exhibited an inverse relationship.
Article
Dentistry, Oral Surgery & Medicine
Ahmed Sleibi, Anwar R. Tappuni, Aylin Baysan
Summary: A dental varnish containing casein phosphopeptide-amorphous calcium phosphate and fluoride showed superior efficacy in reversing/arresting root caries compared to fluoride alone in xerostomic patients over a short-term period. However, there was no significant difference in the long-term efficacy between the two treatments.
Article
Biochemical Research Methods
Amir Wahid, Amir Sohail, Huiyu Wang, Miao Guo, Lu Zhang, Yin Ji, Peng Wang, Hua Xiao
Summary: Extracellular vesicles (EVs) are important in tumor detection, with phosphoproteomics analysis of human saliva helping to identify lung cancer-related candidates. Ti4+-IMAC was shown to be the most efficient method for phosphopeptide isolation from sEVs. By using this approach, over 500 sEV phosphopeptides were identified, providing a comprehensive analysis for the detection of lung cancer non-invasively.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
Rimvydas Falkauskas, Bronius Bakutis, Jurgita Jovaisiene, Gintare Vaiciuliene, Gediminas Gerulis, Sigita Kerziene, Ingrida Jaceviciene, Eugenijus Jacevicius, Violeta Baliukoniene
Summary: This study investigated the metabolites of ZEN in the biological fluids of cows and found that feeding cows a diet without mycotoxins can reduce the concentrations of ZEN in their biological fluids.
Article
Nutrition & Dietetics
Ueli Buetikofer, Rene Badertscher, Carola Blaser-Freiburghaus, Pascal Fuchsmann, Mireille Tena Stern, Philipp A. Kuert, Gregory Pimentel, Kathryn Jane Burton-Pimentel, Nathalie Vionnet, Guy Vergeres
Summary: The identification of molecular biomarkers for the intake of dairy products, such as lactose and its metabolites, indole-3-lactic acid, 3-phenyllactic acid, and 3,5-dimethyloctan-2-one, can help link dietary intake to phenotypic traits. These markers also depend on the fermentation status of the consumed products and can differentiate between yogurt and milk intake. The study also suggests that milk fermentation increases the delivery of free amino acids to the body.
Article
Environmental Sciences
Dyllon. G. G. Randall, Antoine Brison, Kai. M. M. Udert
Summary: It has been found that nitrogen can easily be lost during drying alkalinized urine at high temperatures, and CO2 dissolution may lead to pH decrease and subsequent biological urea hydrolysis. Through experiments and simulations, it was discovered that CO2 dissolution is the main cause of pH decrease, but biological urea hydrolysis prevents pH from dropping below 9.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Materials Science, Multidisciplinary
Nhi Thao-Ngoc Dang, Nam Minh-Phuong Tran, Hoan Ngoc Doan, Nam Cong-Nhat Huynh, Thien-Ly Vu, Tuan-Ngan Tang, An Tran-My Le, Hai-Ly Tran, Phan Thi Thanh Tam, Phuong-Thao Nguyen, Tram Anh-Nguyen Ngoc, Hanh Ta, Thang Phan, Toi Van Vo, Thi-Hiep Nguyen
Summary: This study investigated the effect of synthetic conditions on the physicochemical properties and the remineralization behaviors of BCP materials in artificial saliva. The results showed that the ratio of HA and β-TCP in BCP increased with the increase of pH during the synthesis process. BCP with the highest β-TCP contents, sintered at 800 degrees C, demonstrated fast neutralization capacity to prevent enamel from dissolving.
MATERIALS CHEMISTRY AND PHYSICS
(2023)
Article
Urology & Nephrology
Daniel Rodriguez, Ekaterina Gurevich, Soroush Mohammadi Jouabadi, Eva Maria Pastor Arroyo, Alexander Ritter, Sandrine Estoppey Younes, Carsten A. Wagner, Pedro Henrique Imenez Silva, Harald Seeger, Nilufar Mohebbi
Summary: This study investigated the association between serum sclerostin levels and recurrent kidney stone formation. The findings suggest that sclerostin levels are increased in recurrent kidney stone formers, particularly in women. There was an association between serum sclerostin and the status as a recurrent kidney stone former, independent of hypercalciuria. However, further studies are needed to understand the underlying mechanisms.
CLINICAL KIDNEY JOURNAL
(2023)
Article
Gastroenterology & Hepatology
Dong Ying, Qiyu He, Weikun Tian, Yanling Chen, Xiaoping Zhang, Siling Wang, Chang Liu, Zihao Chen, Yu Liu, Lijuan Fu, Li Yan, Ling Wang, Zimin Tang, Lin Wang, Zizheng Zheng, Ningshao Xia
Summary: This study revealed the origin and diagnostic value of urine Ag in HEV infection, demonstrating that urine Ag has higher diagnostic sensitivity than serum, appears earlier and lasts longer, and shows good concordance with fecal RNA.
Article
Engineering, Biomedical
Patrycja Osak, Joanna Maszybrocka, Julian Kubisztal, Patryk Ratajczak, Bozena Losiewicz
Summary: Calcium phosphate coatings can enhance the osseointegration process and demonstrate good corrosion resistance in experiments. In this study, biomimetic amorphous calcium phosphate (ACP) coatings were successfully prepared by electrodeposition, and their performance on titanium alloy surfaces was characterized and studied.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2021)
Article
Biophysics
Marco Fornasier, Rosa Pireddu, Alessandra Del Giudice, Chiara Sinico, Tommy Nylander, Karin Schillen, Luciano Galantini, Sergio Murgia
Summary: The study explores the encapsulation of natural compounds into nanocarriers to achieve dermal or transdermal release, with the addition of bile salts to enhance skin lipid fluidity. The formulation loaded with bile salts showed superior permeation of natural antioxidants in different skin layers.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Monica Arcos-Hernandez, Polina Naidjonoka, Samuel J. Butler, Tommy Nylander, Henrik Stalbrand, Patric Jannasch
Summary: A series of thermoresponsive glycopolymers, consisting of poly(N-isopropylacrylamide)-co-2-[beta-manno[oligo]syloxy] ethyl methacrylate)s, were synthesized and studied for their aggregation and phase behavior in aqueous solution. The presence of saccharide moieties led to nanoscale structures below the LCST of poly(NIPAm), and a mixture of fractal and disc/globular aggregates above the LCST. The gradual conformational change induced by the pendant beta-mannosyl moieties over a wide temperature range offered temperature-dependent properties different from poly(NIPAm), showing larger colloidal stability in the compacted form.
Article
Chemistry, Physical
Eric Fagerberg, Samuel Lenton, Tommy Nylander, Tilo Seydel, Marie Skepo
Summary: This study investigates the dynamical properties of Histatin 5, an intrinsically disordered protein, under self-crowding conditions. Quasi-elastic neutron scattering and molecular dynamics simulations are used to analyze the diffusion behavior. The results show that diffusion decreases significantly under crowding, possibly due to aggregation at higher protein concentrations. The temperature effects largely follow Stokes-Einstein behavior. Simple geometric considerations fail to predict diffusion rates accurately, while simulations show some agreement with experiments. The scaling law previously used for globular proteins is found inadequate for Histatin 5, highlighting the distinct characteristics of intrinsically disordered proteins.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Chemistry, Physical
Lucrezia Caselli, Andrea Ridolfi, Gaetano Mangiapia, Pierfrancesco Maltoni, Jean-Francois Moulin, Debora Berti, Nina-Juliane Steinke, Emil Gustafsson, Tommy Nylander, Costanza Montis
Summary: This study systematically examines the role of nanoparticle shape in the interaction with lipid assemblies of different symmetry for the first time. It provides new insights on the biological function of phase transitions in response to nanoparticle exposure.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Chemistry, Multidisciplinary
Chaemin Lim, Jacob D. Ramsey, Duhyeong Hwang, Susana C. M. Teixeira, Chi-Duen Poon, Joshua D. Strauss, Elias P. Rosen, Marina Sokolsky-Papkov, Alexander Kabanov
Summary: The study found that the morphology of polymeric micelles significantly affects pharmacological efficacy, with spherical micelles accumulating rapidly in tumor tissue while retaining large amounts of drug, and worm-like micelles accumulating more slowly and only upon releasing significant amounts of drug.
News Item
Biophysics
Tommy Nylander
BIOPHYSICAL JOURNAL
(2022)
Article
Chemistry, Physical
Jordan E. Berger, Susana C. M. Teixeira, Kaelan Reed, Vladimir Razinkov, Christopher J. Sloey, Wei Qi, Christopher J. Roberts
Summary: The effects of high pressure and low temperature on the stability of two different monoclonal antibodies were examined in this study. Partial unfolding was observed for both antibodies under a range of pressure/temperature conditions. Combined use of spectroscopic and scattering techniques provided insights into antibody conformational stability and hysteresis in high-pressure, low-temperature environments.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Chemistry, Multidisciplinary
Adrian Sanchez-Fernandez, Johan Larsson, Anna E. Leung, Peter Holmqvist, Orsolya Czakkel, Tommy Nylander, Stefan Ulvenlund, Marie Wahlgren
Summary: The molecular structure of sugar-based surfactants has a strong impact on their self-assembled structure and dynamics. By manipulating the monomer structure, the behavior of the surfactants, including their dynamics and rheological properties, can be controlled.
Article
Biochemical Research Methods
Jill Trewhella, Patrice Vachette, Jan Bierma, Clement Blanchet, Emre Brookes, Srinivas Chakravarthy, Leonie Chatzimagas, Thomas E. Cleveland, Nathan Cowieson, Ben Crossett, Anthony P. Duff, Daniel Franke, Frank Gabel, Richard E. Gillilan, Melissa Graewert, Alexander Grishaev, J. Mitchell Guss, Michal Hammel, Jesse Hopkins, Qingqui Huang, Jochen S. Hub, Greg L. Hura, Thomas C. Irving, Cy Michael Jeffries, Cheol Jeong, Nigel Kirby, Susan Krueger, Anne Martel, Tsutomu Matsui, Na Li, Javier Perezt, Lionel Porcar, Thierry Prange, Ivan Rajkovic, Mattia Rocco, Daniel J. Rosenberg, Timothy M. Ryan, Soenke Seifert, Hiroshi Sekiguchi, Dmitri Svergun, Susana Teixeira, Aurelien Thureaut, Thomas M. Weiss, Andrew E. Whitten, Kathleen Wood, Xiaobing Zuo
Summary: By collecting SAXS and SANS measurements, consensus scattering profiles for proteins were obtained, which were consistent with theoretical predictions. Optimization of sample purification methods and measurement conditions can improve the quality and precision of the data.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Daniel Papp, Thanya Rukkijakan, Daria Lebedeva, Tommy Nylander, Margareta Sandahl, Joseph S. M. Samec, Charlotta Turner
Summary: The increased interest in using lignin as a feedstock to produce aromatic compounds requires advanced chemical analysis methods for lignin characterization. This study presents a novel method for quantifying lignin dimers using supercritical fluid chromatography with charged aerosol detection. The method was validated using synthesized lignin dimers and found to be effective for quantification. The study also emphasizes the importance of chromatographic peak resolution and introduces a digital peak sharpening technique to address it.
ANALYTICAL CHEMISTRY
(2023)
Article
Polymer Science
Jaeyong Lee, Kevin W. Gao, Neel J. Shah, Cheol Kang, Rachel L. Snyder, Brooks A. Abel, Lilin He, Susana C. M. Teixeira, Geoffrey W. Coates, Nitash P. Balsara
Summary: In this study, we investigated ion transport in electrolytes created by blending different polymers and lithium bis(trifluoromethanesulfonyl)imide (LiTFSI). The addition of salt was found to result in macrophase separation in most polymer blend electrolytes. In general, the ion transport efficiency of polymer blend electrolytes was either lower than or comparable to that of homopolymer electrolytes.
Article
Chemistry, Analytical
Ippei Furikado, Jan Forsman, Tommy Nylander
Summary: The study presents a method for quantifying the adsorption of large particles at the solid-liquid interface using QCM-D and an appropriate viscoelastic model. The validity of the model was confirmed by comparing the results with atomic force microscopy. The study also showed that the recorded dissipation reflects the viscoelastic properties of the layer, and the loss tangent reflects the strength of the particle-substrate contact.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Mohd Ibrahim, Jennifer Gilbert, Marcel Heinz, Tommy Nylander, Nadine Schwierz
Summary: Ionizable lipids, such as MC3, are crucial for the design of LNPs as drug delivery agents. Combining molecular dynamics simulations with experimental data is necessary to understand the internal structure of LNPs. The choice of force field parameters is important for simulation accuracy, and high-quality experimental data is needed for parametrization validation. This study provides parameters for cationic and neutral MC3 compatible with the AMBER Lipid17 force field, and compares different force fields using neutron reflectivity experiments. Accurate force field parameters and experimental validation are essential for reliable simulations.
Article
Chemistry, Analytical
Daniel Papp, Thanya Rukkijakan, Daria Lebedeva, Tommy Nylander, Margareta Sandahl, Joseph S. M. Samec, Charlotta Turner
Summary: A novel method for universal quantification of lignin dimers based on supercritical fluid chromatography with charged aerosol detection (CAD) was developed and applied to quantify lignin dimers in an oil obtained by reductive catalytic fractionation (RCF) of birch sawdust. The applicability of linear regression and quadratic calibration curves for quantification was evaluated and found to be equally appropriate. The response factors of lignin dimers were compared and showed little variation, enabling the use of a single calibrant for these compounds. The importance of chromatographic peak resolution in CAD was stressed and addressed using a digital peak sharpening technique.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jennifer Gilbert, Inna Ermilova, Michihiro Nagao, Jan Swenson, Tommy Nylander
Summary: In this study, the effects of encapsulating two industrially important enzymes in lipid nanoparticles on the dynamics of lipid membranes were investigated using experimental and simulation techniques. The experimental results showed a reduction in membrane dynamics upon enzyme encapsulation, while the simulations revealed that the presence of proteins slowed down the relaxation of lipids, resulting in increased membrane rigidity.