Journal
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
Volume 44, Issue 6, Pages 1193-1207Publisher
SPRINGER
DOI: 10.1007/s40618-020-01405-3
Keywords
Diabetic retinopathy; MicroRNA-486-3p; Bone marrow mesenchymal stem cells; Exosomes; Toll-like receptor 4; NF-kappa b signaling pathway
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Funding
- Medical Science and Technology Research fund project of Guangdong Province [B2018139]
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The study revealed that up-regulation of miR-486-3p induced by BMSC-derived exosomes played a protective role in DR by inhibiting the TLR4/NF-kappa B axis. Upregulating miR-486-3p or down-regulating TLR4 inhibited oxidative stress, inflammation, and apoptosis while promoting proliferation of HG-treated Muller cells. Meanwhile, BMSC-derived exosomes also showed similar protective effects in DR.
Aim Diabetic retinopathy (DR) is a chronic disease causing health and economic burdens on individuals and society. Thus, this study is conducted to figure out the mechanisms of bone marrow mesenchymal stem cells (BMSCs)-induced exosomal microRNA-486-3p (miR-486-3p) in DR. Methods The putative miR-486-3p binding sites to 3 ' untranslated region of Toll-like receptor 4 (TLR4) was verified by luciferase reporter assay. High glucose (HG)-treated Muller cells were transfected with miR-486-3p or TLR4-related oligonucleotides and plasmids to explore theirs functions in DR. Additionally, HG-treated Muller cells were co-cultured with BMSC-derived exosomes, exosomes collected from BMSCs that had been transfected with miR-486-3p or TLR4-related oligonucleotides and plasmids to explore their functions in DR. MiR-486-3p, TLR4 and nuclear factor-kappaB (NF-kappa B) expression, angiogenesis-related factors, oxidative stress factors, viability and apoptosis in HG-treated Muller cells were detected by RT-qPCR, western blot analysis, ELISA, MTT assay and flow cytometry, respectively. Results MiR-486-3p was poorly expressed while TLR4 and NF-kappa B were highly expressed in HG-treated Muller cells. TLR4 was a target of miR-486-3p. Upregulating miR-486-3p or down-regulating TLR4 inhibited oxidative stress, inflammation and apoptosis, and promoted proliferation of HG-treated Muller cells. Meanwhile, BMSC-derived exosomes inhibited oxidative stress, inflammation and apoptosis, and promoted proliferation of HG-treated Muller cells. Restoring miR-486-3p further enhanced, while up-regulating TLR4 reversed, the improvement of exosomes treatment. Conclusion Our study highlights that up-regulation of miR-486-3p induced by BMSC-derived exosomes played a protective role in DR mice via TLR4/NF-kappa B axis repression.
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