Article
Plant Sciences
Aigul Amirova, Symbat Dossymbetova, Yeldana Rysbayeva, Bakdaulet Usenbekov, Arman Tolegen, Alibek Ydyrys
Summary: The aim of this study was to investigate the effect of plant growth regulators on callus induction and in vitro morphogenesis of Paulownia tomentosa and develop an efficient plant regeneration protocol. It was found that the addition of TDZ and NAA stimulated somatic embryogenesis, while the combination of BAP and NAA was optimal for callus induction and multiple plant regeneration. The regenerative capacity of P. tomentosa varied depending on the type of explant, occurring through both callus formation and direct regeneration. Overall, this study successfully developed a reproducible protocol for embryogenic callus formation and multiple shoot induction in vitro, leading to an increased frequency of plant regeneration.
Article
Plant Sciences
Andrei A. Kudriashov, Natalia S. Zlydneva, Elena P. Efremova, Varvara E. Tvorogova, Ludmila A. Lutova
Summary: CLE peptides play a role in plant development and can be regulated by WOX transcription factors. This study identified three CLE peptides that could potentially regulate the MtWOX9-1 gene and stimulate somatic embryogenesis in Medicago truncatula. However, none of these peptides affected the expression of MtWOX9-1 in embryogenic calli. One CLE gene, MtCLE16, was found to decrease somatic embryogenesis intensity when overexpressed, while overexpression of MtCLE08 suppressed the expression of MtWOX13a, a supposed antagonist of somatic embryo development. These findings contribute to the search for new regeneration regulators for plant transformation.
Article
Biotechnology & Applied Microbiology
Yanfang Cai, Lintao Tang, Haixia Chen, Yufan Li, Rong Liu, Jiren Chen
Summary: This study investigates the factors affecting the growth of rose somatic embryos and identifies key factors that can improve the induction rate and efficiency of somatic embryo regeneration.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
Alejandro Hernandez-Soto, Jason Perez, Rebeca Fait-Zuniga, Randall Rojas-Vasquez, Andres Gatica-Arias, Walter Vargas-Segura, Ana Abdelnour-Esquivel
Summary: Gamma ray-mutated rice lines are a solution for introducing genetic variability in already used indica rice varieties. By optimizing the laboratory procedure, the number of chimeras can be reduced and desirable traits can be selected more quickly. In this study, the gene sequences, laboratory cultivation, and radiation conditions were optimized to successfully achieve the regeneration of gamma-ray mutants from indica rice materials.
Article
Cell Biology
Caterina Catalano, Loredana Abbate, Antonio Motisi, Dalila Crucitti, Vincenzo Cangelosi, Antonino Pisciotta, Rosario Di Lorenzo, Francesco Carimi, Angela Carra
Summary: Polyploidy plays an important role in plant adaptation to stresses. In grapevine plants regenerated via somatic embryogenesis, alterations in ploidy level can result in anatomical and morphological changes, such as increased stomata size and chloroplast number, and decreased stomatal index. These observed morphological alterations could be beneficial for breeding grapevine varieties in changing environments.
Article
Horticulture
Elif Aylin Ozudogru, Elif Karlik, Doaa Elazab, Maurizio Lambardi
Summary: This study developed an efficient protocol for in vitro propagation of giant reed biomass through somatic embryogenesis and shoot multiplication. The best explants were immature inflorescences, which provided over 100 embryogenic callus lines. Gelled MS medium supplemented with 1.1 mg/L 2,4-D was found to be the most effective for callus induction. The developed shoots were further multiplied in liquid culture using a temporary immersion system.
Article
Plant Sciences
Mahipal S. Shekhawat, N. Kannan, M. Manokari, S. Priyadharshini
Summary: This study successfully regenerated plantlets of Scaevola taccada through in vitro culture and induced direct somatic embryogenesis from leaf explants, providing a potential method for genetic transformation systems.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Article
Plant Sciences
Milica D. Bogdanovic, Katarina B. Cukovic, Angelina R. Subotic, Milan B. Dragicevic, Ana D. Simonovic, Biljana K. Filipovic, Sladana I. Todorovic
Summary: Somatic embryogenesis (SE) is a crucial method for plant propagation in vitro, which can be induced from different tissues and organs. Research shows that inducing secondary SE at specific concentrations is beneficial for obtaining more and better somatic embryos.
Article
Horticulture
Yu Liu, Ying Zhan, Qiang Fu, Songlin Li, Xinyu Sun, Yaru Wang, Min Yu, Dong Qin, Junwei Huo, Chenqiao Zhu
Summary: This study established an in vitro regeneration system for blue honeysuckle through somatic embryogenesis for the first time, and improved the previously established indirect organogenesis-based regeneration system. By optimizing different culture media, efficient induction of embryogenesis and organogenesis in blue honeysuckle can be achieved. This study provides a powerful tool for rapid propagation, transformation, and genetic improvement of blue honeysuckle.
Review
Biochemistry & Molecular Biology
Janet Juarez-Escobar, Esau Bojorquez-Velazquez, Jose M. Elizalde-Contreras, Jose A. Guerrero-Analco, Victor M. Loyola-Vargas, Martin Mata-Rosas, Eliel Ruiz-May
Summary: Embryogenesis is a crucial developmental program in plants, with research gaps in understanding the mechanisms regulating somatic embryogenesis (SE) and zygotic embryogenesis (ZE). Studies on ZE are limited, while SE research efforts are substantial but disjointed, potentially hindering efficient large-scale plant regeneration. Comprehensive and integrative studies of both SE and ZE are needed to address these challenges and provide a molecular foundation for plant embryogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Zhaodong Hao, Hua Wu, Renhua Zheng, Rui Li, Zeli Zhu, Ya Chen, Ye Lu, Tielong Cheng, Jisen Shi, Jinhui Chen
Summary: The plant peptide hormone PSK can significantly improve the efficiency of somatic embryogenesis and induce this process in recalcitrant genotypes. It helps to maintain ROS homeostasis by decreasing the activity of peroxidases, thus promoting the expression of SE-related genes. Moreover, overexpression of the PSK gene in Arabidopsis enhances somatic embryogenesis and resistance to H2O2 stress.
Article
Plant Sciences
Houssein Wehbi, Camille Soulhat, Halima Morin, Abdelhafid Bendahmane, Pierre Hilson, Oumaya Bouchabke-Coussa
Summary: Plant somatic embryogenesis (SE) is a natural process of vegetative propagation and can be induced in tissue cultures for various purposes. In this study, SE induction in monocots with Brachypodium distachyon as a model was investigated. The researchers identified the earliest stages of cell fate transition and demonstrated that secondary somatic embryos can develop within one week of in vitro culture. The fast SE induction method described in this study could contribute to the mechanistic study of SE processes and shorten the production of transgenic or gene-edited plants.
Article
Horticulture
HongYu Li, FengSong Liu, ShengLi Song, ChunXia Wang, HongMei Sun
Summary: In this study, an efficient in vitro regeneration protocol for Clivia miniata through somatic embryogenesis via a TCL technique was established. This protocol provides a reliable and stable system for sustainable commercial cultivation and conservation of Clivia bioresources.
SCIENTIA HORTICULTURAE
(2022)
Article
Plant Sciences
Snezana Zdravkovic-Korac, Jelena Milojevic, Maja Belic, Dusica Calic
Summary: Species of the genus Aesculus, such as horse chestnut, have medicinal uses and can be used for the treatment of venous insufficiency. Various in vitro propagation methods have been established for mass production of embryos and shoots, including somatic embryogenesis and shoot organogenesis. Additionally, an efficient method for cryopreservation of embryogenic tissue has been developed to ensure constant availability of the plant material.
Article
Multidisciplinary Sciences
Fang Gao, Xi Cao, Caiyun Qin, Shigang Chen, Jufeng Cai, Changbin Sun, Lisheng Kong, Jing Tao
Summary: Embryogenic tissue (ET) is crucial for genetic modification and plant re-generation. This study induced ET from mature zygotic embryos in blue spruce and investigated the effects of different factors on ET proliferation and somatic embryo (SE) maturation. The specific concentrations of 2,4-D and BA during the ET proliferation stage significantly affected both ET growth and the quality of ET for SE maturation. Developing genotype-specific protocols is necessary for efficient production of mature SEs or somatic plants in blue spruce.
SCIENTIFIC REPORTS
(2023)
Correction
Chemistry, Physical
G. Gnanamoorthy, P. Priya, Daoud Ali, M. Lakshmi, Virendra Kumar Yadav
CHEMICAL PHYSICS LETTERS
(2023)
Article
Chemistry, Analytical
Alexander Marimuthu, Saravanan Arunagiri, Arjun Pandian, Pandian Kannaiyan
Summary: TiO2 nanoparticles were prepared at low temperature and subsequently modified with cobalt hexacyanoferrate (CoHCF) through the ion exchange method. The prepared TiO2/CoHCF nanocomposite was characterized and found to exhibit excellent electrocatalytic activity for the oxidation of hydrazine and was used for amperometric determination of hydrazine.
ANALYTICAL SCIENCES
(2023)
Article
Chemistry, Physical
Sundus Azhar, Khuram Shahzad Ahmad, Isaac Abrahams, Shaan Bibi Jaffri, Tenzin Ingsel, Ram K. Gupta, Daoud Ali
Summary: The study investigates the role of phyto-metabolites of Amaranthus viridis in the development of energy-active nanocomplex. The synthesis of doped nanocomplexes using a green route shows economic viability and ecological friendliness. The biomimetically prepared nanoparticles with different doping concentrations exhibit a bandgap range of 2.5 to 3.2 eV. XRD analysis reveals average crystallite sizes of 13.97, 19.64, 16.11, and 11.16 nm for doping concentrations of 2.5, 5, 7.5, and 10%, respectively. The synthesized doped nanoparticles have a porous sheet-like morphology. The material shows promising potential for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) with overpotential values of 154 mV and 445 mV, respectively. Cyclic voltammetry demonstrates the highest capacitance of 287 F/g at 2 mV/s.
Article
Polymer Science
Alexander Marimuthu, Ramesh Periyannan, Daoud Ali, Vasudeva Reddy Minnam Reddy
Summary: The pure semiconductor SnO2 hollow nanosphere (HNS) with a diameter ranging from 300-500 nm and a wall thickness of 40-50 nm was synthesized using a carbon sphere (CS) template-assisted method. The SnO2 nanoparticle size was found to be 20-30 nm. The dimension of the HNS was controlled by adjusting parameters such as SnCl4 concentration, aging time, temperature, and carbon sphere size. The SnO2 HNS exhibited excellent electrocatalytic activity for the reduction of hydrogen peroxide (H2O2) and ethanol, as well as the oxidation of bioactive molecules.
JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS
(2023)
Article
Evolutionary Biology
Hafiz Ishfaq Ahmad, Laraib Jameel, Quratulain Zahra, Jiabin Zhou, Linmiao Li, Xiujuan Zhang, Shakeel Ahmed, Daoud Ali, Gokhlesh Kumar, Aleena Safdar, Farhan Abbas, Jinping Chen
Summary: This study examines the molecular evolution of the IFN epsilon pseudogene in Manis javanica and its role in modulating the innate and specific antiviral immunity in this species. It finds evidence of positive selection in the human and primate IFN epsilon genes, suggesting their importance in the evolution of the immune system. Comparative genomic analysis reveals that the IFN epsilon pseudogene in pangolins originated from a gene duplication event and has lost its protein-coding function, but it may still play a regulatory role in antiviral immunity.
JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH
(2023)
Article
Environmental Sciences
Kumar Pandion, Kantha Deivi Arunachalam, Rajinikanth Rajagopal, Daoud Ali, Saud Alarifi, Soon Woong Chang, Balasubramani Ravindran
Summary: The distribution of heavy metals in seafood intake was studied in the Kalpakkam coastal region. The average concentrations of heavy metals in 40 different fish species were determined. Zinc and copper were found to have higher bioaccumulation index and metal pollution index. Risk assessment calculations showed high values for different age groups. However, the cancer risk did not exceed recommended limits in the region.
MARINE POLLUTION BULLETIN
(2023)
Article
Environmental Sciences
Nisha Choudhary, Virendra Kumar Yadav, Huma Ali, Daoud Ali, Bader O. O. Almutairi, Simona Cavalu, Ashish Patel
Summary: The authors developed a halloysite clay/zinc oxide nanocomposite with photocatalytic activity and high surface area, and demonstrated its efficient removal of methylene blue from aqueous solutions.
Article
Plant Sciences
Anil Patani, Dharmendra Prajapati, Daoud Ali, Haresh Kalasariya, Virendra Kumar Yadav, Jigna Tank, Snehal Bagatharia, Madhvi Joshi, Ashish Patel
Summary: The aim of this research was to isolate halotolerant plant growth promoting Bacillus sp. to promote tomato growth and salt stress resistance. Five selected bacterial strains showed plant growth promoting abilities and improved tomato germination, seedling growth, plant height, dry weight, leaf area, as well as enhanced tolerance to salt stress.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Energy & Fuels
Mahwash Mahar Gul, Khuram Shahzad Ahmad, Andrew Guy Thomas, Daoud Ali
Summary: Spin coating with a single source precursor was used to produce a transparent conductive electrode for a photoelectrochemical cell. Yb2S3:Cu2S:ZnS composite with a crystallite size of 44 nm was discovered using X-ray diffraction analysis. The composite was found to have an optical bandgap of 2.49 eV and comprised Yb, Cu, Zn, and S. Electrical investigations in a photoelectrochemical cell showed that the photocurrent density of the electrode was higher under light and the specific capacitance increased at all scan rates under illumination. The study introduced a useful photoelectrode for renewable energy systems.
Editorial Material
Neurosciences
Dragica Selakovic, Daoud Ali, Aziz Eftekhari, Nemanja Jovicic, Gvozden Luka Rosic
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Md. Abdul Mannan, Amir Yasmin, Umakanta Sarker, Nasimul Bari, Dipanjoli Baral Dola, Hirokazu Higuchi, Sezai Ercisli, Daoud Ali, Saud Alarifi
Summary: Using water extracts of the red seaweed Gracilaria tenuistipitata var. liui as a biostimulant can improve soybean growth and yield, and alleviate the adverse effect of drought stress.
Article
Chemistry, Applied
J. Kabiriyel, R. Jeyanthi, K. Jayakumar, Augustine Amalraj, P. Arjun, A. Shanmugarathinam, G. Vignesh, C. Raja Mohan
Summary: This study successfully utilized the exceptional qualities of chitosan, such as biodegradability, biocompatibility, and nontoxicity, to create a specialized polysaccharide-based composite material. The composite material was prepared by combining curcumin nanoparticles of two different sizes with carboxymethyl chitosan. Characterization techniques including XRD, TEM, UV-Vis, and FTIR were used to analyze the composite. The results showed that the composite with smaller curcumin particle size had higher conductivity and better antimicrobial activity.
CARBOHYDRATE POLYMER TECHNOLOGIES AND APPLICATIONS
(2023)
Article
Gastroenterology & Hepatology
Vasanthakumar Sekar, Ramakrishnan Veerabathiran, Arjun Pandian, Ganesan Sivamani
Summary: This study investigates the effectiveness of targeting liver cancer stem cells through EpCAM gene therapy combined with chemotherapy. The results show that silencing the EpCAM gene and treating cells with XAV939 can decrease the expression of liver cancer stem cell marker CD133 and reduce chemoresistance marker ABCG2. The cells also showed reduced ability to form colonies and spheroids. These findings support the potential of EpCAM-targeted therapy to improve prognosis in hepatocellular carcinoma patients.
EGYPTIAN LIVER JOURNAL
(2023)
Article
Medicine, General & Internal
Bader O. O. Almutairi, Mikhlid H. H. Almutairi, Abdulwahed F. F. Alrefaei, Saad Alkahtani, Saud Alarifi
Summary: This study found that HSPB6 expression is downregulated in colorectal cancer tissues and is associated with tumor progression. Furthermore, HSPB6 is methylated in two colorectal cancer cell lines, and demethylation increases its expression, indicating a mechanistic association between DNA methylation and HSPB6. Therefore, HSPB6 could be a promising biomarker for colorectal cancer diagnosis.
MEDICINA-LITHUANIA
(2023)
Review
Engineering, Environmental
S. Krithika Shree, S. Karthick Raja Namasivayam, Arjun Pandian
Summary: Nanoscale zero-valent iron (nZVI) particles are superior nanomaterials that can undergo various chemical reactions, making them effective for removing toxic substances from water bodies. Antibiotics in pharmaceutical effluents are emerging pollutants, posing a threat to human health and the environment. Advanced methods, including the use of nZVI technology, can be employed to effectively remove drug contaminants and achieve sustainable and green wastewater treatment.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)