Article
Immunology
Carina M. Hall, Nathan E. Stone, Madison Martz, Shelby M. Hutton, Ella Santana-Propper, Lora Versluis, Kieston Guidry, Marielisa Ortiz, Joseph D. Busch, Trevor Maness, Jonathan Stewart, Tom Sidwa, Jay E. Gee, Mindy G. Elrod, Julia K. Petras, Maureen C. Ty, Christopher Gulvik, Zachary P. Weiner, Johanna S. Salzer, Alex R. Hoffmaster, Sarai Rivera-Garcia, Paul Keim, Amanda Kieffer, Jason W. Sahl, Fred Soltero, David M. Wagner
Summary: Burkholderia thailandensis, a bacterium closely related to the pathogen B. pseudomallei, was found in water in Texas and Puerto Rico, as well as soil in Mississippi in the United States. This discovery highlights a potential public health risk, although human infections with B. thailandensis are rare.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Immunology
Zhi-qiang Hu, Qian Li, Zhen-hong Hu, Hai-chao Liu, Cheng-long Rao, Mei-juan Zhang, Yu-pei Xia, Ling Deng, Xu-hu Mao, Yao Fang
Summary: Burkholderia pseudomallei infection inhibits autophagic processes in macrophages by downregulating LIPA expression and upregulating miR-146a expression. Therefore, miR-146a can suppress autophagy by posttranscriptionally suppressing LIPA expression, thereby facilitating the survival of Burkholderia pseudomallei in macrophages.
MICROBIAL PATHOGENESIS
(2021)
Article
Microbiology
Niramol Jitprasutwit, Amporn Rungruengkitkun, Sanisa Lohitthai, Onrapak Reamtong, Nitaya Indrawattana, Nitat Sookrung, Thaniya Sricharunrat, Passanesh Sukphopetch, Narisara Chatratita, Pornpan Pumirat
Summary: Neurological melioidosis caused by Burkholderia pseudomallei can lead to severe neurological damage and increased mortality rate. The involvement of the virulent factor BimA in intracellular infection was investigated in this study. This research is important for understanding the pathogenesis of melioidosis and developing therapeutic strategies.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Matsayapan Pudla, Sucharat Sanongkiet, Peeraya Ekchariyawat, Chularat Luangjindarat, Marisa Ponpuak, Pongsak Utaisincharoen
Summary: TLR9 plays a crucial role in suppressing macrophage killing of Burkholderia pseudomallei by regulating pyroptosis. This finding provides a novel mechanism of TLR9 in the regulation of intracellular bacterial killing by macrophages, which could potentially be leveraged for therapeutic intervention.
MICROBIOLOGY SPECTRUM
(2022)
Review
Immunology
Vanitha Mariappan, Kumutha Malar Vellasamy, Muttiah Barathan, A. S. Smiline Girija, Esaki M. Shankar, Jamuna Vadivelu
Summary: B. pseudomallei infection causes melioidosis with varying severity, diverse pathological manifestations, complex mechanisms of latency and relapse, and the crucial role of immune response in eradicating the pathogen. However, the question of why some individuals are asymptomatic while others are life-threatening remains unanswered.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Yun Heacock-Kang, Ian A. McMillan, Michael H. Norris, Zhenxin Sun, Jan Zarzycki-Siek, Andrew P. Bluhm, Darlene Cabanas, Robert E. Norton, Natkunam Ketheesan, Jeff F. Miller, Herbert P. Schweizer, Tung T. Hoang
Summary: Prokaryotic cell transcriptomics has been limited to mixed or sub-population dynamics and individual cells within heterogeneous populations. The authors develop a 'TRANSITomic' approach to profile transcriptomes of single Burkholderia pseudomallei cells as they transit through host cell infection, revealing dynamic gene-expression flux during transit in host cells and identifying genes that are required for pathogenesis. The 'TRANSITome' approach enables high-resolution understanding of host-pathogen interactions.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Carina M. M. Hall, Nawarat Somprasong, Johannah P. P. Hagen, Roxanne Nottingham, Jason W. W. Sahl, Jessica R. R. Webb, Mark Mayo, Bart J. J. Currie, Yuwana Podin, David M. M. Wagner, Paul Keim, Herbert P. P. Schweizer
Summary: Cefiderocol is a siderophore cephalosporin designed for treating infections caused by beta-lactam and multidrug-resistant Gram-negative bacteria. Resistance in clinical B. pseudomallei isolates from Australia is caused by an uncharacterized mechanism. The PiuA outer membrane receptor plays a major role in cefiderocol nonsusceptibility in isolates from Malaysia.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Chemistry, Multidisciplinary
Michaela Prothiwa, Verena Filz, Sebastian Oehler, Thomas Boettcher
Summary: A novel substrate-based chemical probe was developed against the central quinolone biosynthesis enzyme HmqD in Burkholderia species, leading to the discovery of the first known HmqD inhibitors with single-digit micromolar efficacy in blocking quinolone production in Burkholderia cultures.
Article
Biochemistry & Molecular Biology
Shengyuan Huo, Xiao Li, Shiwei Wang, Pan Wu, Dongqi Nan, Chenglong Rao, Qian Li, Xuhu Mao, Jingmin Yan
Summary: This study investigated the structural elucidation of type B O antigen from a clinical B. pseudomallei strain and the effects of different types of LPS on macrophage differentiation. The O antigen was found to consist of repeating units with specific substitutions. Both type A and type B O antigens could polarize macrophages towards the M1 phenotype. Characterizing different types of O antigen structural motifs is essential for understanding the persistence/survival mechanisms and inflammatory potential of B. pseudomallei.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Microbiology
Kasturi Selvam, Muhammad Fazli Khalid, Khairul Mohd Fadzli Mustaffa, Azian Harun, Ismail Aziah
Summary: Melioidosis is a severe disease caused by Burkholderia pseudomallei, mainly affecting immunocompromised populations such as patients with type 2 diabetes mellitus. Laboratory diagnosis is challenging due to non-specific clinical manifestations and the imperfect sensitivity of culture methods.
Article
Biochemical Research Methods
Cristian D. Loaiza, Naveen Duhan, Matthew Lister, Rakesh Kaundal
Summary: This study developed the genome-scale host-pathogen protein interaction networks between human and B. pseudomallei, identifying potential novel virulence factors and their interacting partners. The findings offer new insights for studying infection mechanisms and developing prevention measures.
BRIEFINGS IN BIOINFORMATICS
(2021)
Letter
Immunology
Himali S. Jayasinghearachchi, Vaithehi R. Francis, Harindra D. Sathkumara, Shivankari Krishnananthasivam, Jayanthi Masakorala, Thilini Muthugama, Aruna D. De Silva, Enoka M. Corea
Summary: A cluster of melioidosis cases occurred in eastern Sri Lanka, with four infections caused by Burkholderia pseudomallei isolates of sequence type 594. Whole-genome analysis revealed genetic diversity among the isolates and showed that the cluster was nonclonal.
EMERGING INFECTIOUS DISEASES
(2021)
Letter
Immunology
Courtney M. Dewart, Francisco A. Almeida, Christine Koval, Scott Nowicki, Jay E. Gee, Mindy Glass Elrod, Christopher A. Gulvik, Johanna S. Salzer, Sietske de Fijter, Lindy Liu
Summary: Phylogenetic analysis of a clinical isolate associated with subclinical Burkholderia pseudomallei infection revealed probable exposure in the British Virgin Islands, where reported infections are limited. Clinicians should consider this geographic distribution when evaluating possible infection among persons with compatible travel history.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Leang-Chung Choh, Guang-Han Ong, Eng-Guan Chua, Kumutha Malar Vellasamy, Vanitha Mariappan, Asif M. Khan, Micheal J. Wise, Kum-Thong Wong, Jamuna Vadivelu
Summary: The study on B.pseudomallei’s bapA gene knockout mutant indicates that the BapA T3SE plays a crucial role in the early stages of bacterial attachment and entry into host cells, with the mutant showing reduced phagocytosis and intracellular replication rates compared to the parental strain. Additionally, the mutant exhibits impaired cell-to-cell spreading capacity without affecting actin tail formation.
PROCESS BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Andrey V. Letarov, Maria A. Letarova, Pavel A. Ivanov, Ilya S. Belalov, Martha R. J. Clokie, Edouard E. Galyov
Summary: Bacteriophages related to phage Bp_AMP1 are a widely distributed group of viruses that infect Burkholderia pseudomallei, the causative agent of melioidosis. They can also infect the nonpathogenic host Burkholderia thailandensis, allowing for experimental work without safety precautions. These viruses may act as natural biocontrol agents that influence the population levels of B. pseudomallei in endemic regions. The cold sensitivity of the lytic growth (CSg) of these phages modulates the effect of viral infection on host populations. Genetic analysis reveals that the CSg phenotype of the AMP1 phage is due to a temperature-sensitive genetic switch encoded by phage ORF3 and ORF14.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Anastasia Polycarpou, Martin J. Holland, Ioannis Karageorgiou, Ayad Eddaoudi, Stephen L. Walker, Sam Willcocks, Diana N. J. Lockwood
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Ashley I. Heinson, Yawwani Gunawardana, Bastiaan Moesker, Carmen C. Denman Hume, Elena Vataga, Yper Hall, Elena Stylianou, Helen McShane, Ann Williams, Mahesan Niranjan, Christopher H. Woelk
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Biochemistry & Molecular Biology
Yong Xu, Jon Cuccui, Carmen Denman, Tripty Maharjan, Brendan W. Wren, Gerd K. Wagner
BIOORGANIC & MEDICINAL CHEMISTRY
(2018)
Article
Microbiology
Rachel J. Parsons, Craig E. Nelson, Craig A. Carlson, Carmen C. Denman, Andreas J. Andersson, Andrew L. Kledzik, Kevin L. Vergin, Sean P. McNally, Alexander H. Treusch, Stephen J. Giovannoni
ENVIRONMENTAL MICROBIOLOGY
(2015)
Article
Microbiology
Carmen C. Denman, Matthew T. Robinson, Andrea M. Sass, Eshwar Mahenthiralingam, Alan R. Brown
Article
Microbiology
Carmen C. Denman, Alan R. Brown
Article
Immunology
Laura E. Marshall, Michelle Nelson, Carwyn H. Davies, Adam O. Whelan, Dominic C. Jenner, Madeleine G. Moule, Carmen Denman, Jon Cuccui, Timothy P. Atkins, Brendan W. Wren, Joann L. Prior
JOURNAL OF IMMUNOLOGY RESEARCH
(2018)
Article
Multidisciplinary Sciences
Priscila Jane Romano de Oliveira Goncalves, Carmen C. Denman Hume, Almir Jose Ferreira, Sarina Tsui, Marcelo Brocchi, Brendan W. Wren, Welington Luiz Araujo
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Hannah Painter, Satria A. Prabowo, Felipe Cia, Lisa Stockdale, Rachel Tanner, Samuel Willcocks, Rajko Reljic, Helen A. Fletcher, Andrea Zelmer
SCIENTIFIC REPORTS
(2020)
Article
Infectious Diseases
S. J. Willcocks, F. Cia, A. F. Francisco, B. W. Wren
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2020)
Article
Microbiology
Valwynne Faulkner, Adrienne Adele Cox, Shan Goh, Annelies van Bohemen, Amanda J. Gibson, Oliver Liebster, Brendan W. Wren, Sam Willcocks, Sharon L. Kendall
Summary: CRISPRi-based gene silencing can be as impactful as gene deletion for studying non-essential genes, and can be applied to other non-essential antimicrobial resistance genes. Designing sgRNAs targeting transcription initiation or early elongation stages has the most impact on rifampicin susceptibility.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Marie-Christine Bartens, Amanda J. Gibson, Graham J. Etherington, Federica Di Palma, Angela Holder, Dirk Werling, Sam Willcocks
Summary: Research suggests that there are differences in the response to mycobacterial antigens between Brown Swiss and Holstein Friesian cattle, potentially due to single nucleotide polymorphisms in the TLR2 gene.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Gathoni Kamuyu, Giuseppe Ercoli, Elisa Ramos-Sevillano, Sam Willcocks, Chidchamai Kewcharoenwong, Pattarachai Kiratisin, Peter W. Taylor, Brendan W. Wren, Ganjana Lertmemongkolchai, Richard A. Stabler, Jeremy S. Brown
Summary: The complement system plays an important role in the defense against bacterial infections, but different strains of A. baumannii show variations in their sensitivity to the complement system. These differences are partially independent of capsule composition or size, and variations in serum resistance among strains are not solely determined by MAC formation on bacterial surfaces.
FRONTIERS IN IMMUNOLOGY
(2022)