Looking back and looking forward: contributions of electron microscopy to the structural cell biology of gametes and fertilization

Acrosin is essential for sperm penetration through the zona pellucida in hamsters

(2020) Michiko Hirose et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells

(2020) David P. Hoffman et al.   SCIENCE

A helical inner scaffold provides a structural basis for centriole cohesion

(2020) Maeva Le Guennec et al.   Science Advances

Haploid male germ cells—the Grand Central Station of protein transport

(2020) Christiane Pleuger et al.   HUMAN REPRODUCTION UPDATE

Structural insights into tetraspanin CD9 function

(2020) Rie Umeda et al.   Nature Communications

In-cell architecture of an actively transcribing-translating expressome

(2020) Francis J. O’Reilly et al.   SCIENCE

Multi-scale 3D Cryo-Correlative Microscopy for Vitrified Cells

(2020) Gong-Her Wu et al.   STRUCTURE

Quantitative 3D Mapping of the Human Skeletal Muscle Mitochondrial Network

(2019) Amy E. Vincent et al.   Cell Reports

Three-dimensional organization of transzonal projections and other cytoplasmic extensions in the mouse ovarian follicle

(2019) Valentina Baena et al.   Scientific Reports

Cryo-SOFI enabling low-dose super-resolution correlative light and electron cryo-microscopy

(2019) Felipe Moser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Breakthroughs and new horizons in reproductive biology of rare and endangered animal species

(2019) Pierre Comizzoli et al.   BIOLOGY OF REPRODUCTION

How Good Can Single-Particle Cryo-EM Become? What Remains Before It Approaches Its Physical Limits?

(2019) Robert M. Glaeser   Annual Review of Biophysics

Axonemal doublet microtubules can split into two complete singlets in human sperm flagellum tips

(2019) Davide Zabeo et al.   FEBS LETTERS

Structure and Function of a Bacterial Gap Junction Analog

(2019) Gregor L. Weiss et al.   CELL

A cryo-FIB lift-out technique enables molecular-resolution cryo-ET within native Caenorhabditis elegans tissue

(2019) Miroslava Schaffer et al.   NATURE METHODS

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes

(2019) Chun So et al.   SCIENCE

PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

(2019) Sergey Gorelick et al.   eLife

Comparative analysis of mammalian sperm ultrastructure reveals relationships between sperm morphology, mitochondrial functions and motility

(2019) Ni-Hao Gu et al.   Reproductive Biology and Endocrinology

Mineralized scale patterns on the cell periphery of the chrysophyte Mallomonas determined by comparative 3D Cryo-FIB SEM data processing

(2019) Marcel Hörning et al.   JOURNAL OF STRUCTURAL BIOLOGY

Odf2 haploinsufficiency causes a new type of decapitated and decaudated spermatozoa, Odf2-DDS, in mice

(2019) Chizuru Ito et al.   Scientific Reports

Direct visualization of degradation microcompartments at the ER membrane

(2019) Sahradha Albert et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cryo-Electron Microscopy Reveals That Sperm Modification Coincides with Female Fertility in the Mosquito Aedes aegypti

(2019) Jade M. Noble et al.   Scientific Reports

Stepped vitrification technique for human ovarian tissue cryopreservation

(2019) Ellen Cristina Rivas Leonel et al.   Scientific Reports

A convolutional autoencoder approach for mining features in cellular electron cryo-tomograms and weakly supervised coarse segmentation

(2018) Xiangrui Zeng et al.   JOURNAL OF STRUCTURAL BIOLOGY

Asymmetric distribution and spatial switching of dynein activity generates ciliary motility

(2018) Jianfeng Lin et al.   SCIENCE

A novel atypical sperm centriole is functional during human fertilization

(2018) Emily L. Fishman et al.   Nature Communications

A lumenal interrupted helix in human sperm tail microtubules

(2018) Davide Zabeo et al.   Scientific Reports

Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly

(2018) Hiroshi Yamaguchi et al.   eLife

Ultrastructural characteristics of human oocytes vitrified before and after in vitro maturation

(2017) Yolanda SEGOVIA et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Cellular electron cryo tomography and in situ sub-volume averaging reveal the context of microtubule-based processes

(2017) Michael Grange et al.   JOURNAL OF STRUCTURAL BIOLOGY

Convolutional neural networks for automated annotation of cellular cryo-electron tomograms

(2017) Muyuan Chen et al.   NATURE METHODS

In situ structure of trypanosomal ATP synthase dimer reveals a unique arrangement of catalytic subunits

(2017) Alexander W. Mühleip et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhanced FIB-SEM systems for large-volume 3D imaging

(2017) C Shan Xu et al.   eLife

Glycerol kinase-like proteins cooperate with Pld6 in regulating sperm mitochondrial sheath formation and male fertility

(2017) Yuxi Chen et al.   Cell Discovery

Formation and Dissociation of Sperm Bundles in Monotremes

(2016) B. Nixon et al.   BIOLOGY OF REPRODUCTION

Volume scanning electron microscopy for imaging biological ultrastructure

(2016) Benjamin Titze et al.   BIOLOGY OF THE CELL

Visualizing the molecular sociology at the HeLa cell nuclear periphery

(2016) J. Mahamid et al.   SCIENCE

Cryoprotectant-free vitrification of human spermatozoa in new artificial seminal fluid

(2016) A. Agha-Rahimi et al.   Andrology

Transmission electron microscopy in molecular structural biology: A historical survey

(2015) J. Robin Harris   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Developing 3D SEM in a broad biological context

(2015) A. KREMER et al.   JOURNAL OF MICROSCOPY

A focused ion beam milling and lift-out approach for site-specific preparation of frozen-hydrated lamellas from multicellular organisms

(2015) Julia Mahamid et al.   JOURNAL OF STRUCTURAL BIOLOGY

Electron cryotomography of vitrified cells with a Volta phase plate

(2015) Yoshiyuki Fukuda et al.   JOURNAL OF STRUCTURAL BIOLOGY

Mitochondrial reticulum for cellular energy distribution in muscle

(2015) Brian Glancy et al.   NATURE

Structural analysis of multicellular organisms with cryo-electron tomography

(2015) Jan Harapin et al.   NATURE METHODS

In situ structural analysis of Golgi intracisternal protein arrays

(2015) Benjamin D. Engel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Organelle reorganization in bovine oocytes during dominant follicle growth and regression

(2015) D. Dadarwal et al.   Reproductive Biology and Endocrinology

A molecular census of 26S proteasomes in intact neurons

(2015) S. Asano et al.   SCIENCE

Structurally Distinct Ca2+ Signaling Domains of Sperm Flagella Orchestrate Tyrosine Phosphorylation and Motility

(2014) Jean-Ju Chung et al.   CELL

Ultrastructure of immature and mature human oocytes after cryotop vitrification

(2014) Maria Grazia PALMERINI et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Conventional transmission electron microscopy

(2014) Mark Winey et al.   MOLECULAR BIOLOGY OF THE CELL

Juno is the egg Izumo receptor and is essential for mammalian fertilization

(2014) Enrica Bianchi et al.   NATURE

Structural mechanism of the dynein power stroke

(2014) Jianfeng Lin et al.   NATURE CELL BIOLOGY

Volta potential phase plate for in-focus phase contrast transmission electron microscopy

(2014) R. Danev et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The dynamics of sperm cooperation in a competitive environment

(2014) H. S. Fisher et al.   PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Ultrastructural changes in oocytes during folliculogenesis in domestic mammals

(2014) Fernanda Paulini et al.   Journal of Ovarian Research

Is EM dead?

(2013) G. Knott et al.   JOURNAL OF CELL SCIENCE

Cryo FIB-SEM: Volume imaging of cellular ultrastructure in native frozen specimens

(2013) Andreas Schertel et al.   JOURNAL OF STRUCTURAL BIOLOGY

Three-Dimensional Structure of the Bovine Sperm Connecting Piece Revealed by Electron Cryotomography1

(2012) Puey Ounjai et al.   BIOLOGY OF REPRODUCTION

Conserved structural motifs in the central pair complex of eukaryotic flagella

(2012) Blanca I. Carbajal-González et al.   Cytoskeleton

Ultrastructure of human mature oocytes after vitrification

(2012) M.A. Khalili et al.   EUROPEAN JOURNAL OF HISTOCHEMISTRY

The effect of vitrification on ultrastructure of human in vitro matured germinal vesicle oocytes

(2012) Abbas Shahedi et al.   European Journal of Obstetrics & Gynecology and Reproductive Biology

Comparative structural analysis of eukaryotic flagella and cilia from Chlamydomonas, Tetrahymena, and sea urchins

(2012) Gaia Pigino et al.   JOURNAL OF STRUCTURAL BIOLOGY

Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography

(2012) A. Rigort et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrastructure of cilia and flagella - back to the future!

(2011) Cathy Fisch et al.   BIOLOGY OF THE CELL

Cryo-electron tomography reveals conserved features of doublet microtubules in flagella

(2011) D. Nicastro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The origins and evolution of freeze-etch electron microscopy

(2011) John E. Heuser   Microscopy

Preservation of fertility in young cancer patients: contribution of transmission electron microscopy

(2010) Alessandra Camboni et al.   REPRODUCTIVE BIOMEDICINE ONLINE

Character, distribution and biological implications of ice crystallization in cryopreserved rabbit ovarian tissue revealed by cryo-scanning electron microscopy

(2009) R. G. Gosden et al.   HUMAN REPRODUCTION

Structural changes of the zona pellucida during fertilization and embryo development

(2008) Giuseppe Familiari   Frontiers in Bioscience-Landmark

Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa

(2008) Sinan Ozkavukcu et al.   JOURNAL OF ASSISTED REPRODUCTION AND GENETICS

Zona Pellucida Glycoproteins

(2008) Paul M. Wassarman   JOURNAL OF BIOLOGICAL CHEMISTRY

An Improved Cryogen for Plunge Freezing

(2008) William F. Tivol et al.   MICROSCOPY AND MICROANALYSIS