Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 41, Issue -, Pages 14-18Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2016.03.003
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Funding
- National Science Foundation [DEB EF-0747397-, DEB-1321792]
- Department of Energy's Biological Systems Science Division, Program in Genomic Science
- Division Of Environmental Biology
- Direct For Biological Sciences [1241094, 1241115] Funding Source: National Science Foundation
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Growth and mortality of microorganisms have been characterized through DNA stable isotope probing (SIP) with O-18-water in soils from a range of ecosystems. Conventional SIP has been improved by sequencing a marker gene in all fractions retrieved from an ultracentrifuge tube to produce taxon density curves, which allow estimating the atom percent isotope composition of each microbial taxon's genome. Very recent advances in SIP with O-18-water include expansion of the technique to aquatic samples, investigations of microbial turnover in soil, and the first use of O-18-water in RNA-SIP studies.
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