Article
Immunology
Delong Chen, Feifan Chu, Gangyu Zhang, Qingqing Wang, Ying Li, Meng Zhang, Qi He, Junzheng Yang, Haibin Wang, Ping Sun, Jiake Xu, Peng Chen
Summary: The natural compound 12-deoxyphorbol 13-acetate (DPA) was found to inhibit osteoclast differentiation and function, potentially serving as a promising treatment for osteoporosis. Mechanistically, DPA suppressed RANKL-induced downstream signaling pathways and showed efficacy in an osteoporosis mice model.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Cell Biology
Chang Wan Kim, Yongdae Yoon, Moon Young Kim, Soon Koo Baik, Hoon Ryu, Il Hwan Park, Young Woo Eom
Summary: This study investigated the antifibrotic effect of protein kinase C (PKC) in myofibroblasts and its involvement in Hippo pathway activation. The results show that PKC delta exerts an antifibrotic effect by inhibiting the Hippo pathway in hepatic stellate cells (HSCs). PKC delta and YAP can be potential therapeutic targets for fibrotic diseases.
Article
Nutrition & Dietetics
Su-Ji Park, Do-Wan Kim, Seong-Ryeong Lim, Junghee Sung, Tae Hoon Kim, In Sun Min, Chang-Hyung Choi, Sei-Jung Lee
Summary: The study showed that Kaempferol can inhibit the cytotoxicity and inflammatory response induced by TPA, by preventing the generation of ROS and JNK phosphorylation, as well as inhibiting the phosphorylation of NF-kappa B and I kappa B alpha, to reduce the expression of cleaved caspase-3 and IL-1 beta.
Article
Immunology
Johanna Veh, Charlotte Mangold, Anja Felsen, Carolin Ludwig, Lisa Gerstner, Peter Reinhardt, Hubert Schrezenmeier, Dorit Fabricius, Bernd Jahrsdoerfer
Summary: PMA/TPA, a phorbol ester similar to DAG, can enhance the differentiation of pre-activated B cells into GraB cells induced by IL-21. PMA/TPA may be a safe and feasible alternative for ex-vivo manufacturing of GraB cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Plant Sciences
Pei-Sheng Lee, Yi-Shiou Chiou, Pin-Yu Chou, Kalyanam Nagabhushanam, Chi-Tang Ho, Min-Hsiung Pan
Summary: The study demonstrated that HPSB inhibits TPA-induced skin inflammation and DMBA/TPA-induced skin carcinogenesis in mice by down-regulating key proteins involved in the processes. Additionally, HPSB also suppresses the expression of certain genes involved in carcinogen activation, suggesting its potential as a chemopreventive agent.
Article
Dermatology
Chandler W. Rundle, Hope R. Rietcheck, Jalal Maghfour, Sam Dercon, Jon Fernandez, Peter Lio, Robert P. Dellavalle, Mayumi Fujita, Helena Yardley
Summary: This study assessed the effects of a topical cannabinoid product on a dermatitis mouse model. The results showed that the formulation significantly reduced ear edema, indicating its anti-inflammatory properties. However, its effects were not as robust as potent topical corticosteroids.
Article
Cell & Tissue Engineering
Yujia Zhang, Fengjun Qiu, Tingjie Ye, Sau Har Lee, Jiatuo Xu, Lingyan Jia, Rui Zeng, Xiaoling Wang, Xudong Hu, Xiaofeng Yan, Hua Li, Yanlin Lu, Rilei Jiang, Wei Xu
Summary: This study identified a signature of 6 resistance genes associated with chemoresistance in lung cancer patients. The EREG/ErbB pathway was found to regulate stemness and maintain chemoresistance through the ERK signaling pathway in NSCLC. Targeting EREG/ErbB could effectively resolve the issue of chemoresistance.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Mitsuhiro Kinoshita, Atsushi Yamada, Kiyohito Sasa, Kaori Ikezaki, Tatsuo Shirota, Ryutaro Kamijo
Summary: The study revealed that PMA regulates Npnt gene expression in osteoblasts through the PKC alpha and c-Jun/c-Fos pathway, leading to suppression of cell differentiation. This regulation is time- and dose-dependent, and can be reversed by using PKC signal inhibitor or siRNA transfection.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Fatih Karadeniz, Jung Hwan Oh, Youngwan Seo, Jiho Yang, Hyunjung Lee, Chang-Suk Kong
Summary: The current study investigated the anti-melanogenic effect of Quercetin 3-O-galactoside (Q3G) and revealed its underlying mechanism of action in B16F10 melanoma cells. Q3G treatment downregulated melanogenesis-related enzymes and transcription factor MITF, inhibited cAMP/PKA and MAPK signaling pathways, and reduced melanin production. These findings suggest that Q3G has potential as a cosmetic ingredient for treating hyperpigmentation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Golam Iftakhar Khandakar, Yoichi Miyamoto, Ryosuke Satoh, Kenta Kishimoto, Mingzuo Xie, Mengyu Shih, Teruaki Takasaki, Genzoh Tanabe, Masahiro Oka, Reiko Sugiura
Summary: The anti-cancer compound GT-7 stimulates ERK phosphorylation, leading to growth inhibition and apoptosis in pancreatic cancer cells. GT-7 induces phosphorylated ERK accumulation in the nucleus, which precedes and underlies apoptosis induction in T3M4 cells.
Article
Chemistry, Medicinal
Kien Tran, Robin Van den Hauwe, Xavier Sainsily, Pierre Couvineau, Jerome Cote, Louise Simard, Marco Echevarria, Alexandre Murza, Alexandra Serre, Lea Theroux, Sabrina Saibi, Lounes Haroune, Jean-Michel Longpre, Olivier Lesur, Mannix Auger-Messier, Claude Spino, Michel Bouvier, Philippe Sarret, Steven Ballet, Eric Marsault
Summary: Side-chain-constrained amino acids were used to modify apelin-13 (Ape13), resulting in analogues with higher affinity for the apelin receptor (APJ). Substitutions at the Pro12 and Phe13 positions, particularly using 1Nal, Trp, Aia, D-Tic, Db(z)g, and D-alpha-Me-Tyr(OBn) residues, proved to be beneficial in enhancing binding affinity and signaling profile. Compounds 47 and 53 emerged as the most potent Ape13 analogues, with improved pharmacokinetic properties and activation of the G alpha(12) pathway.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Katarzyna Rafa-Zablocka, Agnieszka Zelek-Molik, Beata Tepper, Piotr Chmielarz, Grzegorz Kreiner, Michal Wilczkowski, Irena Nalepa
Summary: This study found that chronic repeated stress affects the expression of proteins related to the G alpha 12/13 signaling pathway in the brain, with beta 1AR blockade showing potential therapeutic effects. These findings provide insight into the molecular mechanisms of stress-related neuropsychiatric diseases.
PHARMACOLOGICAL REPORTS
(2021)
Article
Immunology
Sarah G. Groft, Nancy Nagy, W. Henry Boom, Clifford Harding
Summary: This study demonstrates that ERK signaling in response to TLR2 stimulation has different effects on macrophages and dendritic cells, potentially influencing their functions in the immune system by regulating IL-12/23p40 expression.
INFECTION AND IMMUNITY
(2021)
Article
Immunology
Sonia Giambelluca, Matthias Ochs, Elena Lopez-Rodriguez
Summary: THP-1 monocytes can be differentiated to macrophages by incubation with PMA, and a 24-hour rest period allows the down-regulation of pro-IL-1 beta without affecting the ability to respond to a second stimulus through activation of the inflammasome.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Jin-Young Park, Hee Jae Lee, Eun-Taek Han, Jin-Hee Han, Won Sun Park, Yong-Soo Kwon, Wanjoo Chun
Summary: The anti-allergic properties of 3,4,5-Trihydroxycinnamic acid (THC) were investigated, and it was found that THC could inhibit degranulation and histamine release in mast cells by suppressing the MAPKs/ NF-kappa B signaling pathway.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Materials Science, Biomaterials
Kadir Ozaltin, Elif Vargun, Antonio Di Martino, Zdenka Capakova, Marian Lehocky, Petr Humpolicek, Natalia Kazantseva, Petr Saha
Summary: Plasma treatment is used to increase the hydrophilicity of porous polylactic acid and facilitate the immobilization of fucoidan for medicated wound dressings.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Eva Safarikova, Jiri Ehlich, Stanislav Stritesky, Martin Vala, Martin Weiter, Jiri Pachernik, Lukas Kubala, Jan Vitecek
Summary: Organic semiconductors have great potential in regenerative medicine due to their ability to control stem cell differentiation. A study found that the conductive polymer PEDOT:PSS platform is beneficial for the differentiation of mouse embryonic stem cells and can generate higher electrical current.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Lenka Musilova, Eva Achbergerova, Lenka Vitkova, Roman Kolarik, Martina Martinkova, Antonin Minarik, Ales Mracek, Petr Humpolicek, Jiri Pecha
Summary: In this study, a novel cytocompatible material suitable for extrusion-based 3D printing was designed. Hydrogels were prepared by cross-linking gelatins with dextran polyaldehyde, exhibiting desirable rheological behavior and cell-friendly environment. The hydrogels showed strong shear-thinning behavior, quick gelation, and formed self-supported porous structures. Additionally, the hydrogels were non-cytotoxic and allowed homogeneous cell distribution within the printed scaffolds.
Article
Immunology
Silvie Kremserova, Anna Kocurkova, Michaela Chorvatova, Anna Klinke, Lukas Kubala
Summary: The study reveals that MPO plays a role in regulating PMN cell death by enhancing cell membrane permeabilization and phosphatidylserine surface exposure, thereby extending the lifespan of PMNs.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Vojtech Pavlik, Veronika Machalova, Martin Cepa, Romana Sinova, Barbora Safrankova, Jaromir Kulhanek, Tomas Drmota, Lukas Kubala, Gloria Huerta-Angeles, Vladimir Velebny, Kristina Nesporova
Summary: The study found that HA-atRA upregulated gene expression similarly to atRA, but HA-atRA activated the expression of cholesterol metabolism genes. The results showed that HA-atRA removed cholesterol from the cells and induced cholesterol synthesis to replenish it. Additionally, HA-C18:1 also removed cholesterol from the cells, indicating that the cholesterol removal stemmed from the amphiphilic nature of these derivatives.
Review
Biochemistry & Molecular Biology
Anna Kocurkova, Kristina Nesporova, Miriam Sandanusova, Michaela Kerberova, Katerina Lehka, Vladimir Velebny, Lukas Kubala, Gabriela Ambrozova
Summary: Formation of peritoneal adhesions is a major complication following intra-abdominal surgery, primarily caused by activation of cells and deposition of extracellular matrix. Hyaluronan plays an important role in this process.
Article
Biochemistry & Molecular Biology
Marketa Kadleckova, Katerina Skopalova, Barbora Ptoskova, Erik Wrzecionko, Eliska Dadova, Karolina Kocourkova, Ales Mracek, Lenka Musilova, Petr Smolka, Petr Humpolicek, Antonin Minarik
Summary: The pseudo 3D hierarchical structure mimicking in vivo microenvironment was prepared using phase separation method. It was found that the structured surface had a significant impact on cell proliferation and morphology, with the most remarkable effect observed on embryonic stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Asma Abdulkareem, Aya E. Abusrafa, Sifani Zavahir, Salma Habib, Patrik Sobolciak, Marian Lehocky, Hana Pistekova, Petr Humpolicek, Anton Popelka
Summary: Hydrophobic fibrous slippery liquid-infused porous surfaces (SLIPS) can prevent biofilm formation and exhibit antibacterial activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Pawel M. Szczepanik, Andrey A. Mikhaylov, Ondrej Hylse, Roman Kucera, Petra Dadova, Marek Necas, Lukas Kubala, Kamil Paruch, Jakub Svenda
Summary: We report a new synthetic strategy for the flexible preparation of forskolin-like molecules. Stereoselective Michael addition and ring-opening metathesis paired with ring closure were key steps in achieving the concise assembly of the tricyclic core. Late-stage functionalization sequences transformed the tricyclic intermediates into a set of different forskolin-like molecules.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Radim Jarousek, Antonia Mikulova, Petra Dad'ova, Petr Taus, Terezia Kurucova, Karla Plevova, Boris Tichy, Lukas Kubala
Summary: Single-cell transcriptomics is a powerful tool for investigating the biological characteristics of cells. In this study, human T cells were analyzed using single-cell transcriptomic approach with support of labelled antibodies and comprehensive bioinformatics analysis. The results revealed differences in expression profiles among different T cell subpopulations, providing insights into the importance of each step of the analysis and aiding in a more practical understanding of the results.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Materials Science, Biomaterials
Marek Cernik, Kamila Polakova, Lukas Kubala, Andrea Viteckova Wunschova, Anna Mac Gillavry Danylevska, Michaela Peskova, Jan Vitecek
Summary: On-chip vascular microfluidic models using PDMS have been widely used to study cardiovascular diseases. However, the hydrophobic surface of PDMS needs to be modified for biological applications. This study introduces a high-frequency low-pressure air-plasma surface modification method that improves the surface properties of seamless channels within a PDMS microfluidic chip, enabling efficient adhesion and long-term culture of vascular endothelial cells under flow conditions.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Engineering, Biomedical
L. Vitkova, N. Kazantseva, L. Musilova, P. Smolka, K. Valaskova, K. Kocourkova, M. Humenik, A. Minarik, P. Humpolicek, A. Mracek, I. Smolkova
Summary: In this study, a magneto-responsive hydrogel based on hyaluronan was fabricated, which exhibited good mechanical properties and thermosensitivity. It was found that the hydrogel could rapidly heat under exposure to low amplitude radio frequency alternating magnetic field. In addition, the hydrogel showed cytocompatibility and 3D printability, making it suitable for biomedical applications.
APL BIOENGINEERING
(2023)
Article
Immunology
Petra Dadova, Antonia Mikulova, Radim Jarous, Michaela Chorvatova, Stjepan Uldrijan, Lukas Kubala
Summary: The adenylyl cyclase (AC) signaling pathway is suggested to play a key role in regulating immune system functions. This study demonstrates that cyclic adenosine mono-phosphate (cAMP) affects the differentiation of T helper (Th) cells, enhancing Th1 cell phenotype, supporting Th2 differentiation but negatively affecting cell metabolism, and suppressing Th17 immunophenotype while upregulating CXCL13.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Meeting Abstract
Immunology
E. Krivakova, R. Bosnjakovic, L. Kubala, O. Vasicek
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Meeting Abstract
Immunology
D. Rubanova, J. Bryja, S. Skoroplyas, A. Libanska, T. Etrych, L. Kubala
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
