Article
Microbiology
Younjung Kim, Maura Carrai, Marcus H. Y. Leung, Jaime Chin, Jun Li, Patrick K. H. Lee, Julia A. Beatty, Dirk U. Pfeiffer, Vanessa R. Barrs
Summary: The study found that the feline bladder microbiome is typically sparse, with various phyla, families, and genera identified. Different urotypes were associated with disease status, with the Escherichia-Shigella-predominant urotype being common in CKD cases. The microbial composition of CKD cases was more similar to that of E. coli PUC cases than to controls, indicating potential implications for the development of clinical infections.
Article
Immunology
Thijs ten Doesschate, Sander Kuiper, Cees van Nieuwkoop, Robert-Jan Hassing, Tom Ketels, Suzan P. van Mens, Wouter van den Bijllaardt, Akke K. van der Bij, Suzanne E. Geerlings, Ad Koster, Evert L. Koldewijn, Judith Branger, Andy I. M. Hoepelman, Cornelis H. van Werkhoven, Marc J. M. Bonten
Summary: Fosfomycin is noninferior to ciprofloxacin as oral step-down treatment for febrile urinary tract infections (fUTIs) caused by E. coli in women, but its use is associated with more gastrointestinal events.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Microbiology
Amanda Brumwell, Granger Sutton, Paul M. M. Lantos, Kate Hoffman, Felicia Ruffin, Lauren Brinkac, Thomas H. H. Clarke, Mark D. D. Adams, Vance G. G. Fowler Jr, Derrick E. E. Fouts, Joshua T. T. Thaden
Summary: This study aims to further understand the risk factors, clinical outcomes, and bacterial genetics associated with ST131 bloodstream infections. A cohort study was conducted in which 88 out of 227 patients with E. coli bloodstream infection were infected with ST131. There was no significant difference in in-hospital mortality between patients with ST131 and non-ST131 infections overall, but ST131 was associated with higher mortality in patients with urinary tract infections.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Katarzyna Werbel, Dorota Jankowska, Anna Wasilewska, Katarzyna Taranta-Janusz
Summary: The study analyzed the increasing resistance of bacteria in children with UTI over the years, identified parameters related to antibiotic sensitivity using the CART method, and provided guidance for antibiotic therapy to inhibit increasing antibiotic resistance.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Infectious Diseases
Guadalupe Miranda-Novales, Karen Flores-Moreno, Yolanda Lopez-Vidal, Samuel Ponce de Leon-Rosales
Summary: This study evaluated the antimicrobial susceptibility patterns of Escherichia coli and Klebsiella pneumoniae, and found that multidrug-resistant bacteria represented 61% of all isolates.
Review
Nutrition & Dietetics
Reeta Ala-Jaakkola, Arja Laitila, Arthur C. Ouwehand, Liisa Lehtoranta
Summary: Urinary tract infections (UTIs) are a common bacterial disease worldwide, and antibiotic resistance is a major concern. D-mannose, a dietary supplement, shows promise as an alternative treatment for UTIs, reducing the risk and recurrence.
Article
Immunology
Rongmei Yao, Xin Mao, Yingli Xu, Xue Qiu, Lirun Zhou, Yaxin Wang, Bo Pang, Mengping Chen, Shan Cao, Lei Bao, Yanyan Bao, Shanshan Guo, Limin Hu, Haijiang Zhang, Xiaolan Cui
Summary: This study reveals the potential mechanism by which Vaccaria segetalis polysaccharides (VSP) interfere with the adhesion and invasion of uropathogenic Escherichia coli (UPEC). VSP inhibits the expression of bacterial adhesion genes and regulates specific molecules in host epithelial cells, reducing the ability of bacteria to invade the host.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Mohamed Kettani Halabi, Fatima Azzahra Lahlou, Idrissa Diawara, Younes El Adouzi, Rabiaa Marnaoui, Rachid Benmessaoud, Imane Smyej
Summary: ESBL-EC infections pose challenges due to limited treatment options, increased hospital costs, reduced microbiological and clinical responses, and a threat to patient's life. Majority of ESBL-EC isolates were from female adult patients over 50, resistant to third-generation cephalosporins and quinolones, but sensitive to carbapenems and fosfomycin.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Sandra J. Aedo, Juechun Tang, Mark P. Brynildsen
Summary: The study showed that stationary-phase Escherichia coli are not susceptible to nitrofurantoin (NIT), but supplementation with glucose under conditions where essential nutrients were absent can increase susceptibility. This insensitivity is mainly due to a low abundance of reducing equivalents, while metabolites can resensitize the bacteria.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Adriana Belas, Catia Marques, Juliana Menezes, Luis Telo da Gama, Patricia Cavaco-Silva, Constanca Pomba
Summary: This study aimed to compare ESBL/pAmpC-producing E. coli strains causing community-acquired UTI in companion animals and humans. The study found that companion animals and humans shared two multidrug-resistant high-risk clonal lineages: ST131 and ST648. The study also reported two newly discovered clonal lineages in humans with community-acquired UTI in Portugal.
Article
Biochemistry & Molecular Biology
Nabi Jomehzadeh, Morteza Saki, Khadijeh Ahmadi, Golshan Zandi
Summary: This study aimed to determine the prevalence of quinolone resistance and the presence of plasmid-mediated quinolone resistance (PMQR) genes among extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates. The results showed a high resistance rate to nalidixic acid and ciprofloxacin among ESBL-producing isolates. The most frequent PMQR gene was aac(6')-Ib-cr variant. The coexistence of aac(6')-Ib-cr variant and qnrB was the most widely distributed genotype among quinolone resistance isolates.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Infectious Diseases
Michaela Tutone, Truls E. Bjerklund Johansen, Tommaso Cai, Shazad Mushtaq, David M. Livermore
Summary: Urinary tract infections (UTIs) are common worldwide, especially among women. A study investigated the susceptibility of current urinary isolates to fosfomycin and other antibiotics, and found that Escherichia coli was the most common uropathogen isolated and highly susceptible to fosfomycin, nitrofurantoin, and mecillinam.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Microbiology
Lotte Jakobsen, Carina Vingsbro Lundberg, Niels Frimodt-Moller
Summary: Ciprofloxacin is highly effective in clearing urine and kidney tissue for fully susceptible E. coli, but not for low-level resistant strains. PKPD correlations show a higher correlation between AUC/MIC and effect in urine and kidneys, while %T->MIC shows a lower correlation.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Public, Environmental & Occupational Health
Jiazhen Guo, Ran Duan, Dan Zhang, Peng Zhang, Shuai Qin, Yajuan Fang, Yingna Sun, Lianhe Lu, Huaiqi Jing, Xin Wang, Rongmeng Jiang, Biao Kan
Summary: This study reports a unique case of community-acquired NDM-5 Escherichia coli urinary tract infection (UTI) following COVID-19. The UTI persisted for at least 45 days. Genomic analyses revealed the presence of two NDM-5 strains, with identical chromosomal background but distinct plasmids. This case suggests the presence of diverse resistance genes in the human body, and the continuous evolution of drug-resistant strains during infection, possibly originating from the intestinal tract.
Article
Multidisciplinary Sciences
Lalitha Maniam, Kumutha Malar Vellasamy, Teng Aik Ong, Cindy Shuan Ju Teh, Kartini Abdul Jabar, Vanitha Mariappan, Vallikkannu Narayanan, Jamuna Vadivelu, Vinod Pallath
Summary: This study aimed to investigate the distribution of virulence determinants and antimicrobial resistance among different phylogroups of UPEC isolated from various clinical categories of cUTI and ASB E. coli isolates. The findings showed that the virulence factors and antibiotic resistance of UPEC isolates varied in different cUTI categories, and specific genotypic characteristics of UPEC were associated with severe UTI.
Article
Biochemical Research Methods
Swaine L. Chen
Article
Multidisciplinary Sciences
Mohammad Arifuzzaman, Yuvon R. Mobley, Hae Woong Choi, Pradeep Bist, Cristina A. Salinas, Zachary D. Brown, Swaine L. Chen, Herman F. Staats, Soman N. Abraham
Article
Microbiology
Colin W. Russell, Rashmi Sukumaran, Lu Ting Liow, Balamurugan Periaswamy, Shazmina Rafee, Yuemin C. Chee, Swaine L. Chen
Article
Multidisciplinary Sciences
Swaine L. Chen, Ying Ding, Anucha Apisarnthanarak, Shirin Kalimuddin, Sophia Archuleta, Sharifah Faridah Syed Omar, Partha Pratim De, Tse Hsien Koh, Kean Lee Chew, Nadia Atiya, Nuntra Suwantarat, Rukumani Devi Velayuthan, Joshua Guo Xian Wong, David C. Lye
SCIENTIFIC REPORTS
(2019)
Article
Genetics & Heredity
Swaine L. Chen, Nico M. Temme
G3-GENES GENOMES GENETICS
(2020)
Article
Microbiology
Aung-Hein Aung, Kala Kanagasabai, Jocelyn Koh, Pei-Yun Hon, Brenda Ang, David Lye, Swaine L. Chen, Angela Chow
Summary: The study found that CPE colonization was higher in acute-care hospitals compared to intermediate-term and long-term-care facilities within a healthcare network. Risk factors for CPE colonization varied between different types of facilities, highlighting the need for targeted screening and precautionary measures to address this issue. Genomic analysis revealed possible intra-hospital transmission but no evidence of transmission from hospitals to long-term care facilities.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Correction
Multidisciplinary Sciences
Swaine L. Chen, Ying Ding, Anucha Apisarnthanarak, Shirin Kalimuddin, Sophia Archuleta, Sharifah Faridah Syed Omar, Partha Pratim De, Tse Hsien Koh, Kean Lee Chew, Nadia Atiya, Nuntra Suwantarat, Rukumani Devi Velayuthan, Joshua Guo Xian Wong, David C. Lye
Summary: The paper has been amended and the revised version can be accessed through a link at the top of the paper.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Kurosh S. Mehershahi, Swaine L. Chen
Summary: The study revealed that three non-phasevariable Type I RMSs in Escherichia coli have no measurable impact on gene expression, in vivo virulence, or any of 1190 in vitro growth phenotypes. This suggests that there may be other nonregulatory methylation systems like these, expanding our understanding of the potential role of RMSs in bacterial evolution.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Food Science & Technology
Man Ling Chau, Swaine L. Chen, Min Yap, Kyaw Thu Aung, Sri Harminda Pahm Hartantyo, Ahmad Zhafir Ariff, Sreenivasulu Kilari, Grisez Luc, Wei Ling Tan, Brian Z. Y. Tan, Wai Kwan Wong, Ramona Alikiiteaga Gutierrez, Lee Ching Ng, Timothy Barkham
Summary: The study identified the presence of Group B Streptococcus (GBS) in seafood and meat beyond freshwater fish and observed higher contamination rates in wet market samples compared to supermarket samples. The findings emphasize the need for further genomic analysis of human, animal, and food isolates to understand the potential of non-ST283 GBS causing foodborne infections.
Article
Multidisciplinary Sciences
Varnica Khetrapal, Liyana Ayub Ow Yong, Sylvester J. M. Lim, Swaine L. Chen
Summary: This study introduces the concept of mass allelic exchange (MAE) in Escherichia coli, which allows for classical linkage analysis that connects genotypes to phenotypes. The utility of MAE is demonstrated through virulence-related gain-of-function screens, providing insights into the natural allelic variation in E. coli and potentially other bacteria.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Rafi Rashid, Zeus Jaren Nair, Dominic Ming Hao Chia, Kelvin Kian Long Chong, Amaury Cazenave Gassiot, Stewart A. Morley, Doug K. Allen, Swaine L. Chen, Shu Sin Chng, Markus R. Wenk, Kimberly A. Kline
Summary: The bacterial cell membrane plays an important role in cell envelope synthesis, protein secretion, virulence factor assembly, and defense against antimicrobial peptides. The multiple peptide resistance factor (MprF) enzyme, found in several Gram-positive pathogens, attaches cationic amino acids to anionic phospholipids in the cell membrane to resist antimicrobial peptide killing. This study aimed to understand the broader lipidomic and functional roles of the MprF enzyme in Enterococcus faecalis. The findings revealed that MprF depletion led to changes in lipid homeostasis, membrane fluidity, protein secretion, and biofilm formation, highlighting the importance of MprF in global lipid regulation and cellular physiology.
Review
Fisheries
Olga L. M. Haenen, Ha Thanh Dong, Truong Dinh Hoai, Margaret Crumlish, Iddya Karunasagar, Timothy Barkham, Swaine L. Chen, Ruth Zadoks, Andreas Kiermeier, Bing Wang, Esther Garrido Gamarro, Masami Takeuchi, Mohammad Noor Amal Azmai, Belen Fouz, Rolando Pakingking, Zeng Wei Wei, Melba G. Bondad-Reantaso
Summary: Tilapia culture is a significant source of income and nutrition for rural families. However, intensification and global warming have led to bacterial disease outbreaks in tilapia farms, threatening sustainable production. This review provides an overview of common and emerging bacterial pathogens, diseases, diagnostics, and the risks of antimicrobial resistance in tilapia.
REVIEWS IN AQUACULTURE
(2023)
Article
Infectious Diseases
Hong Ming Tan, Ambika C. Lall, Jussi Keppoa, Swaine L. Chen
Summary: Current systemic strategies to combat antimicrobial resistance (AMR) are focused on reducing antibiotic use, but they have been generally ineffective. These strategies also create negative incentives for pharmaceutical companies to invest in new antibiotic research and development, which exacerbates the problem. This paper proposes a new systemic strategy called "antiresistics," which refers to any intervention that reduces resistance rates in pathogen populations. The use of antiresistics can have positive effects on existing antibiotic efficacy and the development of new antibiotics.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Kar Hui Ong, Kyaw Thu Aung, Sharon C. M. Chan, Swaine L. Chen, Lee Ching Ng, Kitiya Vongkamjan
Summary: This study revealed the draft genome sequences of 21 Salmonella isolates obtained from poultry production chains in Hat Yai City, Songkhla Province, Thailand, highlighting environmental contamination and cross-contamination among poultry through inanimate surfaces in the environment.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)