Journal
ONCOTARGETS AND THERAPY
Volume 13, Issue -, Pages 8715-8722Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/OTT.S262293
Keywords
NSCLC; HIF1A-AS2; miR-153-5p; S100A14
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Background: Long noncoding RNA (IncRNA) plays a critical role in initiating lung cancer. This study aims to research the function and mechanism of IncRNA HIF1A-AS2 in regulating non-small cell lung cancer (NSCLC) progression. Methods: qRT-PCR was used to analyze gene expression. The CCK-8 assay was performed to detect cell proliferation. The Transwell assay was conducted to examine cell migration and invasion. A Caspase3 activity detection kit was utilized to analyze apoptosis. The luciferase reporter assay was carried out to research interactions of HIF1A-AS2, miR-153-5p and S100A14. Results: HIF1A-AS2 expression was raised in NSCLC tissues and cell lines. The HIF1AAS2 level was increased in advanced NSCLC tumor tissues. High HIF1A-AS2 expression was related to poor prognosis. HIF1A-AS2 knockdown decreased proliferation, migration and invasion while promoting apoptosis. HIF1A-AS2 was the sponge for miR-153-5p, and miR-153-5p targeted S100A14. H1F1A-AS2 promoted S100A14 expression through regulating miR-153-5p. Conclusion: The HIF1A-AS2/miR-153-5p/S100A14 axis plays a crucial role in promoting NSCLC progression.
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