How to Grow a Tree: Plant Voltage-Dependent Cation Channels in the Spotlight of Evolution

Phylogenetic analysis can be a powerful tool for generating hypotheses regarding the evolution of physiological processes. Here, we provide an updated view of the evolution of the main cation channels in plant electrical signalling: the Shaker family of voltage-gated potassium channels and the two-pore cation (K+) channel (TPC1) family. Strikingly, the TPC1 family followed the same conservative evolutionary path as one particular subfamily of Shaker channels (K-out) and remained highly invariant after terrestrialisation, suggesting that electrical signalling was, and remains, key to survival on land. We note that phylogenetic analyses can have pitfalls, which may lead to erroneous conclusions. To avoid these in the future, we suggest guidelines for analyses of ion channel evolution in plants.

Automated summary

Phylogenetic analysis is a powerful tool for studying the evolution of physiological processes in plants. Research on two families of ion channels in plant electrical signaling revealed their conservative evolution on land, highlighting the importance of electrical signaling for survival. To prevent erroneous conclusions, guidelines for analyzing ion channel evolution in plants have been suggested.