Journal
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Volume 130, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2020.115985
Keywords
Sample preparation; Nucleic acid; Cell lysis; Liquid-liquid extraction; Solid-phase extraction; Microfluidic-based extraction; Sequence-specific extraction
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Funding
- Chemical Measurement and Imaging Program at the National Science Foundation [CHE-1709372]
- Alice Hudson Professorship at ISU
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The extraction of nucleic acids (NA) is an essential component of NA analysis. Without sufficient extraction and purification, contaminants co-existing with NAs often inhibit enzymatic amplification causing poor reproducibility and sensitivity. Numerous advancements in NA sampling in recent years have led to improvements in extraction yields from complex matrices and the reduction of analysis times. Perhaps most notable is the push towards miniaturization and automation to facilitate point-of-care testing. This article reviews the advancements and current trends in NA sample preparation including cell lysis and NA extraction. (C) 2020 Elsevier B.V. All rights reserved.
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