Article
Cell Biology
Tiejian Nie, Kai Tao, Lin Zhu, Lu Huang, Sijun Hu, Ruixin Yang, Pingyi Xu, Zixu Mao, Qian Yang
Summary: This study demonstrates the critical role of chaperone-mediated autophagy (CMA) in maintaining proper mitochondria dynamics. Loss of this regulatory control may occur in Parkinson's disease (PD), leading to impaired mitochondria function. Increasing CMA activity promotes MARCHF5 turnover, reduces mitochondria fragmentation, and alleviates mitochondrial dysfunction under oxidative stress.
Review
Immunology
Lilian Gomes de Oliveira, Yan de Souza Angelo, Antonio H. Iglesias, Jean Pierre Schatzmann Peron
Summary: Neuroinflammatory and neurodegenerative diseases are major public health issues worldwide, with mitochondrial dysfunction playing a significant role. Regulation of mitochondrial dynamics is essential for CNS health maintenance, impacting the development and progression of neuroinflammation.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell Biology
Mathew George, Matthan Tharakan, John Culberson, Arubala P. Reddy, P. Hemachandra Reddy
Summary: Nrf2 is a crucial transcription factor that regulates gene expression in healthy and disease states. It plays a vital role in oxidative stress, mitochondrial function, autophagy, and is closely associated with aging and neurodegenerative diseases.
AGEING RESEARCH REVIEWS
(2022)
Review
Physiology
Tong Zhang, Minh D. A. Luu, Amalia M. M. Dolga, Ulrich L. M. Eisel, Martina Schmidt
Summary: Alzheimer's disease (AD) and Parkinson's disease (PD) are the most prevalent neurodegenerative disorders worldwide, impacting millions of people's life expectancy and quality. Recent research suggests overlapping mechanisms may underlie AD and PD, with novel cell death mechanisms, such as parthanatos, netosis, lysosome-dependent cell death, senescence, and ferroptosis, being modulated by cAMP signaling via PKA and Epac. This review focuses on the overlapping mechanisms between AD and PD, specifically in relation to cAMP signaling and the pharmacology of cAMP signaling pathways.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Paulina Bastian, Jaroslaw Dulski, Anna Roszmann, Dagmara Jacewicz, Alicja Kuban-Jankowska, Jaroslaw Slawek, Michal Wozniak, Magdalena Gorska-Ponikowska
Summary: Mitochondria, known as the power house of the cell, play crucial roles in cell pathophysiology, participating in various physiological activities. Dysregulation of mitochondrial dynamics may lead to the development of diseases. 2-methoxyestradiol (2-ME) has the potential to affect cell growth and mitochondrial function by inhibiting nitric oxide synthase (NOS) production and oxidative stress.
Review
Geriatrics & Gerontology
Afzal Misrani, Sidra Tabassum, Li Yang
Summary: Mitochondria play a pivotal role in cell viability, with changes in morphology and dysfunction being closely linked to the pathogenesis of diseases like Alzheimer's. Restoring mitochondrial function can potentially delay the progression of Alzheimer's disease.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
JunHyuk Woo, Hyesun Cho, YunHee Seol, Soon Ho Kim, Chanhyeok Park, Ali Yousefian-Jazi, Seung Jae Hyeon, Junghee Lee, Hoon Ryu
Summary: The brain requires a significant amount of energy, with mitochondria serving as the energy generator within cells and playing a crucial role in the pathogenesis of neurodegenerative disorders.
Review
Biology
Giusy Tassone, Arian Kola, Daniela Valensin, Cecilia Pozzi
Summary: Alzheimer's disease is a neurodegenerative disorder affecting millions of people worldwide, with no effective treatments currently available. Research using proteomics and redox proteomics has identified potential drug targets in altered proteins and pathways, particularly in mitochondrial proteins related to metabolism, ATP generation, oxidative stress, and apoptosis. Despite the lack of effective drugs, compounds targeting mitochondria have shown promising results in research.
Article
Neurosciences
Tania Das Banerjee, Kelly Reihl, Maryann Swain, Mariana Torres, Ruben K. Dagda
Summary: Alzheimer's disease is characterized by progressive memory loss and cognitive decline, with mitochondrial fragmentation and neurodegeneration being key pathological features. Decreased PKA signaling in neurons is associated with these pathological changes.
MOLECULAR NEUROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Cadiele Oliana Reichert, Debora Levy, Sergio P. Bydlowski
Summary: The human body has biological redox systems that can prevent or reduce damage caused by increased oxidative stress throughout life. The PON enzymes, particularly PON1, are associated with neurodegenerative diseases and play a crucial role in their development.
Review
Biochemistry & Molecular Biology
Xiaojia Ren, D. Allan Butterfield
Summary: Parkinson's disease (PD) is the second most common age-related neurodegenerative disease globally, impacting quality of life significantly as people live longer. Genetically modified PD rodent models are crucial for studying mechanisms and potential therapies. The MJFF funded the generation of a PINK1 KO rat model for familial PD, aiming to improve understanding of PD etiology and treatment strategies.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Stefan Wendt, Sora Johnson, Nicholas L. Weilinger, Christopher Groten, Stefano Sorrentino, Jonathan Frew, Lucy Yang, Hyun B. Choi, Haakon B. Nygaard, Brian A. MacVicar
Summary: The inter-relationship between microglia dynamics and oxidative stress in dystrophic neurites at Alzheimer's Disease plaques may contribute to the pathological changes in neurons. Microglia preferentially enwrap neurites exhibiting the greatest degree of oxidative stress, and depletion of microglia leads to increased oxidative stress in neurites. These findings suggest a complex role of microglia in Alzheimer's Disease, with both neuroprotective and neurotoxic components.
Article
Chemistry, Analytical
Madalina M. Barsan, Victor C. Diculescu
Summary: The study aimed to develop a biosensor for investigating the 20S proteasome activity and inhibition using electrochemical methods. The biosensor showed high sensitivity and low detection limit, capable of analyzing the enzyme's multi-catalytic activity and response to inhibitors. This cost-effective analytical method offers advantages compared to traditional approaches.
Review
Biochemistry & Molecular Biology
Pamela J. Urrutia, Daniel A. Borquez, Marco Tulio Nunez
Summary: Iron accumulation and neuroinflammation are closely related in neurodegenerative diseases like AD and PD, where inflammatory mediators such as hepcidin and nitric oxide play a significant role in disrupting iron homeostasis and contributing to neuronal death.
Review
Biochemistry & Molecular Biology
Anna De Gaetano, Lara Gibellini, Giada Zanini, Milena Nasi, Andrea Cossarizza, Marcello Pinti
Summary: Mitochondrial dysfunction is a key characteristic of aging, with mitophagy playing a crucial role in eliminating damaged mitochondria. Oxidative stress is a major factor in aging, triggering mitophagy to remove dysfunctional mitochondria. However, chronic oxidative stress can lead to mitochondrial damage that overwhelms the autophagic pathways, disrupting the delicate balance between mitophagy, ROS production, and mitochondrial damage, which can accelerate the aging process.
Article
Medicine, General & Internal
Viviana Manzulli, Giulia Scioscia, Giulio Giganti, Maria Rosaria Capobianchi, Donato Lacedonia, Lorenzo Pace, Dora Cipolletta, Pasquale Tondo, Rosella De Nittis, Valeria Rondinone, Luigina Serrecchia, Antonio Parisi, Domenico Galante, Sergio Lo Caputo, Teresa Antonia Santantonio, Damiana Moschetta, Vitangelo Dattoli, Antonio Fasanella, Maria Pia Foschino Barbaro
Summary: The study conducted viral culture on hospitalized patients with COVID-19, showing that patients who clinically recovered for at least three days and still tested positive on nasopharyngeal swab had viral clearance at viral culture, suggesting they do not remain contagious.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Virology
Valeria Rondinone, Lorenzo Pace, Antonio Fasanella, Viviana Manzulli, Antonio Parisi, Maria Rosaria Capobianchi, Angelo Ostuni, Maria Chironna, Elisabetta Caprioli, Maria Labonia, Dora Cipolletta, Ines Della Rovere, Luigina Serrecchia, Fiorenza Petruzzi, Germana Pennuzzi, Domenico Galante
Summary: The study aimed to evaluate the protection level of sera from 12 patients infected and later healed in Apulia Region, Italy with Covid-19 between March and November 2020, against the new VOC 202012/01 variant by seroneutralization assay. The study showed that antibodies produced in subjects infected with variants of Sars-CoV-2 strain before the appearance of the English one seem to have a neutralizing power also against this variant.
Article
Virology
Loredana Capozzi, Angelica Bianco, Laura Del Sambro, Domenico Simone, Antonio Lippolis, Maria Notarnicola, Graziano Pesole, Lorenzo Pace, Domenico Galante, Antonio Parisi
Summary: Genomic analysis of SARS-CoV-2 strains in South Eastern Italy revealed that the circulating viral variants mainly originated from the second wave of infections, which are being closely monitored globally. The study underscores the importance of genome sequencing for epidemiological surveillance of the spread of new variants.
Article
Microbiology
Domenico Galante, Viviana Manzulli, Luigina Serrecchia, Pietro Di Taranto, Martin Hugh-Jones, M. Jahangir Hossain, Valeria Rondinone, Dora Cipolletta, Lorenzo Pace, Michela Iatarola, Francesco Tolve, Angela Aceti, Elena Poppa, Antonio Fasanella
Summary: In 2011, 11 anthrax outbreaks occurred in six districts of Bangladesh. The study found that contaminated feed and water might also play an important role in the epidemiology of anthrax.
Article
Engineering, Environmental
G. La Rosa, P. Mancini, G. Bonanno Ferraro, C. Veneri, M. Iaconelli, L. Lucentini, L. Bonadonna, S. Brusaferro, D. Brandtner, A. Fasanella, L. Pace, A. Parisi, D. Galante, E. Suffredini
Summary: A nested RT-PCR assay was developed to detect key mutations of SARS-CoV-2 variants, showing potential for rapid screening of clinical samples and detection of variants in sewage. The study highlights the importance of wastewater surveillance in exploring SARS-CoV-2 diversity and distribution.
Review
Chemistry, Medicinal
Vidyasagar Naik Bukke, Moola Archana, Rosanna Villani, Gaetano Serviddio, Tommaso Cassano
Summary: Synthetic cannabinoids are a type of psychoactive substances that have rapidly evolved with diverse structural modifications, potentially leading to serious adverse health effects. These substances are sprayed on harmless natural herbs to mimic the euphoric effect of Cannabis, and due to their stronger potency, they have been increasingly associated with intoxications and deaths.
Article
Biology
Domenico Galante, Viviana Manzulli, Adelia Donatiello, Antonio Fasanella, Barbara Chirullo, Massimiliano Francia, Valeria Rondinone, Luigina Serrecchia, Lorenzo Pace, Michela Iatarola, Michela Tarantino, Rosanna Adone
Summary: In this study, the Bacillus anthracis vaccine strain Sterne 34F2 was cultured to obtain a secretome with a high concentration of non-degraded PA(83), which was used as an antigen in a Complement Fixation Test (CFT) to monitor antibody production. The results showed that the PAS-based CFT and commercial ELISA kit yielded similar results in terms of specificity and sensitivity, and the Sterne 34F2 vaccine induced a strong antibody response to PA(83) in vaccinated rabbits.
Article
Virology
Luigia Trabace, Lorenzo Pace, Maria Grazia Morgese, Isabel Bianca Santo, Domenico Galante, Stefania Schiavone, Dora Cipolletta, Anna Maria Rosa, Pierluigi Reveglia, Antonio Parisi, Paolo Tucci, Giovanni Pepe, Rodolfo Sacco, Maria Pia Foschino Barbaro, Gaetano Corso, Antonio Fasanella
Summary: The Delta variant has raised concerns about its ability to evade SARS-CoV-2 vaccines. This study evaluated the neutralizing response of 172 Italian healthcare workers three months after receiving the Comirnaty vaccine. The results showed that the vaccine provides sustained neutralizing antibody activity against the Alpha variant, but it is less effective against the Gamma and even less against the Delta variants.
Article
Biology
Maurizio Guastalegname, Valeria Rondinone, Giuseppe Lucifora, Alfredo Vallone, Laura D'Argenio, Giovanni Petracca, Antonia Giordano, Luigina Serrecchia, Viviana Manzulli, Lorenzo Pace, Antonio Fasanella, Domenico Simone, Dora Cipolletta, Domenico Galante
Summary: This report describes three cases of human cutaneous anthrax, one complicated by meningitis, all linked to a single infected bullock. Microbiological examination and PCR testing on patients and the infected bullock led to accurate diagnosis and treatment. Collaboration between human and veterinary medicine proved successful in managing human anthrax cases.
Article
Biochemistry & Molecular Biology
Tommaso Cassano, Flavia Giamogante, Silvio Calcagnini, Adele Romano, Angelo Michele Lavecchia, Francesca Inglese, Giuliano Paglia, Vidyasagar Naik Bukke, Antonino Davide Romano, Marzia Friuli, Fabio Altieri, Silvana Gaetani
Summary: In this study, a mouse model of Alzheimer's disease (AD) was used to investigate the expression of PDIA3, an endoplasmic reticulum chaperone, in brain regions affected by AD pathology. The results showed an age-dependent increase in PDIA3 levels in AD mice, and a significant reduction in PDIA3 at an early age compared to non-AD mice. Immunohistochemistry analysis revealed a correlation between the levels of A beta and PDIA3 proteins in all brain regions analyzed. The findings suggest that altered PDIA3 levels may play a role in AD pathology and could be a potential therapeutic target.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Maria Emanuela Mancini, Alessandra Alessiani, Adelia Donatiello, Antonella Didonna, Luigi D'Attoli, Simona Faleo, Gilda Occhiochiuso, Francesco Carella, Pietro Di Taranto, Lorenzo Pace, Valeria Rondinone, Annita Maria Damato, Rosa Coppola, Carmine Pedarra, Elisa Goffredo
Summary: This study aimed to determine the prevalence and antibiotic susceptibility of Salmonella spp. and Vibrio spp. in bivalve molluscs and the environmental multidrug resistance (MDR). The results showed one Salmonella strain with multidrug resistance and 41.3% of the Vibrio strains with resistance to certain antibiotics. Continuous monitoring and establishment of interpretative criteria are recommended.
Review
Medicine, General & Internal
Rosanna Villani, Pierluigi Lupo, Moris Sangineto, Antonino Davide Romano, Gaetano Serviddio
Summary: Non-alcoholic fatty liver disease (NAFLD) is a common chronic disease affecting up to 38% of the general population, with various risk factors such as genetic predisposition and high-calorie diets. Liver biopsy, the traditional method for NAFLD diagnosis, has limitations due to potential complications and sampling variability. Non-invasive assessment methods using serum biomarkers and liver stiffness quantification have emerged as a new frontier in NAFLD management. This article provides a state-of-the-art summary on ultrasound-based techniques and their optimal cut-off values for staging liver fibrosis in NAFLD patients.
Review
Biology
Alfredo Fabrizio Lo Faro, Diletta Berardinelli, Tommaso Cassano, Gregory Dendramis, Eva Montanari, Angelo Montana, Paolo Berretta, Simona Zaami, Francesco Paolo Busardo, Marilyn Ann Huestis
Summary: We conducted a comprehensive literature review on intoxications and fatalities related to New Psychoactive Substances (NPS) during the COVID-19 pandemic. The pandemic led to changes in drug consumption patterns, with some drugs being consumed less and an increase in the use of NPS. Synthetic opioids, synthetic cannabinoids, and synthetic cathinones were the most commonly abused NPS classes, and the combined consumption of several NPS classes accounted for 30% of all cases. It is important to consider that the reported cases may be an underestimate due to the impact of the pandemic on forensic toxicology capabilities.
Article
Medicine, General & Internal
Nicola Di Fazio, Matteo Scopetti, Giuseppe Delogu, Donato Morena, Alessandro Santurro, Luigi Cipolloni, Gaetano Serviddio, Luigi Papi, Paola Frati, Emanuela Turillazzi, Vittorio Fineschi
Summary: Healthcare-related homicidal cases can be challenging to investigate due to the lack of material evidence. This study presents a multidisciplinary approach to a case involving 14 deaths from abnormal bleeding caused by heparin overdose. The investigation involved a thorough review of clinical documentation, exhumation and autopsy of the deceased patients, and histopathological, immunohistochemical, and toxicological investigations. The analysis confirmed the presence of fatal hemorrhagic episodes and demonstrated the link between heparin overdose and the recorded deaths. The importance of a multidisciplinary approach in healthcare-related judicial cases is highlighted.
Article
Health Care Sciences & Services
G. Diella, G. Caggiano, F. Apollonio, F. Triggiano, P. Stefanizzi, F. Fasano, L. Pace, V. Marcotrigiano, D. P. Sorrenti, G. T. Sorrenti, D. Galante, M. T. Montagna
Summary: The COVID-19 emergency has emphasized the significance of prevention systems and environmental microbiological monitoring in responding to epidemics and other health threats. The use of automated no-touch room disinfection systems improves the effectiveness of terminal disinfection by reducing reliance on operators. This study examines the potential SARS-CoV-2 contamination of surfaces in commercial services and evaluates the effectiveness of ozone treatment on the virus.
ANNALI DI IGIENE MEDICINA PREVENTIVA E DI COMUNITA
(2023)
