Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 321, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.128479
Keywords
G-quadruplex DNA; Fluorescence; AIEgen; Mitochondrion; Living cells imaging
Funding
- National Natural Science Foundation of China [21877082, 21572147]
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Inspired by the mechanism of aggregation induced emission, two derivatives of thiazole orange (TPE-mTO and Ph-TO) were rationally designed and prepared in this work. Their selectivity and sensitivity towards G-quadruplex were studied by fluorescence titration, gel analysis, and circular dichroism (CD) experiments. TPE-mTO could selectively lights-up G-quadruplex DNA structures with no conformational transition, while Ph-mTO could not distinguish the G-quadruplex DNA structure from other nucleic acid, which probably owing to the pocket size and shape of the G-quadruplex DNA only could hinder the rotation of TPE moiety. Then the speculation was verified by molecular docking, TPE-mTO could adopt an appropriate pose in 3 ' and 5 ' binding pockets of CM22 (G-quadruplex), the multiple interaction between TPE-mTO and CM22 do hinder the rotation of TPE moiety and lead to strong fluorescence. In addition, the detection limit (DL) of TPE-mTO towards CM22 (prefolded Gquadruplex) was found as low as 4.1 nM. With the help of TPE-mTO, the G-quadruplex DNA structures in mitochondrion can be easily and quickly tracked without further washing operations. Overall, the probe we developed (TPE-mTO) was a simple but powerful tool for studying G-quadruplex structures.
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