Article
Multidisciplinary Sciences
M. Jalal Uddin, Nabil H. Bhuiyan, Joon S. Shim
Summary: By integrating programmable and disposable microfluidic pump and valve on the device, we successfully transformed the traditional 96-well ELISA assay into a point-of-care testing device, achieving individualized ELISA testing for each patient. This device significantly reduced the consumption of reagents and assay time, greatly improving the detection sensitivity, making ELISA testing more efficient and convenient.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Anna Toldra, Georgios Chondrogiannis, Mahiar M. Hamedi
Summary: This paper presents a paper-based microfluidic device, called MEL-PAD, which can perform enzyme-linked assays. The MEL-PAD system utilizes a wash-free sandwich coupling and vertical flow to detect analytes. It achieves efficient washing and color change detection using nitrocellulose and a chromogenic substrate. The MEL-PAD demonstrates high sensitivity and versatility for quantifying various analytes. The article also shows the potential of MEL-PAD in detecting DNA from Staphylococcus epidermidis.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemical Research Methods
Saichon Sumantakul, Vincent T. Remcho
Summary: This work presents a novel method for fabricating enclosed channel porous-media microfluidic analytical devices using selective laser ablation. By sandwiching a porous material between polymeric films and selectively ablating the porous substrate, microfluidic structures can be easily created within the devices. The method is versatile and can be applied to a wide range of porous materials and polymeric films, resulting in enclosed microfluidic devices with different fluid flow characteristics. The fabricated devices demonstrated their utility in quantitative assays for albumin, glucose, and cholesterol in human serum. This unique and scalable method not only provides protection against contamination and fluid evaporation, but also offers a commercial fabrication approach for porous-media analytical devices.
Article
Biotechnology & Applied Microbiology
Yunxuan Chen, Yaxi Hu, Xiaonan Lu
Summary: This study developed a hybrid paper/polymer-based microfluidic device for the point-of-need identification of Campylobacter jejuni. The device integrates paper-based DNA extraction, isothermal nucleic acid amplification, and lateral flow detection. It has high specificity and sensitivity, making it a promising tool for rapid detection of Campylobacter in food.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Mechanics
H. Asadi, M. Pourjafar-Chelikdani, N. P. Khabazi, K. Sadeghy
Summary: This study numerically investigates the spontaneous imbibition of shear-dependent fluids in a paper-based diagnostic kit using the modified Richards equation. The results show that the microstructure of the absorbent pad and its contact angle strongly influence the average velocity at the test line. The shear-thinning behavior of the test fluid is found to shorten the constant-velocity regime on the nitrocellulose membrane, but it can still be extended by manipulating the contact angle and/or choosing appropriate microstructure.
Article
Chemistry, Analytical
M. Jalal Uddin, Nabil H. Bhuiyan, Jun H. Hong, Joon S. Shim
Summary: A fully automated optofluidic device was developed for enzyme-linked immunosorbent assay using a smartphone-based optical platform. The platform successfully monitored liquid movement in the reaction chamber by measuring changes in light intensity, achieving a low detection limit for human cardiac biomarker detection.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Amer Charbaji, Hojat Heidari-Bafroui, Constantine Anagnostopoulos, Mohammad Faghri
Summary: The paper presents a simple and cost-effective paper-based microfluidic device for detecting nitrate in water. By incorporating recent advancements in paper-based technology suitable for nitrate detection, the device achieved significant improvement in nitrate detection compared to previous methods.
Article
Chemistry, Analytical
Miguel Vazquez-Alvarado, ShiLu Vanasupa, Elide Herrera Valdez, Alyssa M. Pama, Maile J. Crowder, Linda Vanasupa, Nathaniel W. Martinez, Andres W. Martinez
Summary: Microfluidic paper-based analytical devices (microPADs) are a promising platform for point-of-care assays. This study evaluated and compared 33 chromogenic substrate solutions for horseradish peroxidase (HRP) in terms of their analytical performance. The results showed that TMB and OPD substrates achieved the best overall performance. The findings of this study will guide the selection and formulation of substrate solutions for future paper-based assays involving HRP.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Biotechnology & Applied Microbiology
Shamraja S. Nadar, Pravin D. Patil, Manishkumar S. Tiwari, Dinesh J. Ahirrao
Summary: Low-cost paper-based analytical devices are a generation of portable lab-on-chip designs that provide an innovative platform for on/off-site analysis of target analytes, especially in rural and remote areas. These devices offer exciting fundamental features such as ease of fabrication, rapid operation, and precise interpretations, while incorporating enzymes to improve analytical performance. Additionally, they are compact, portable, easy-to-use, and do not require additional sophisticated equipment.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Review
Chemistry, Medicinal
Atchara Lomae, Pattarachaya Preechakasedkit, Kanyapat Teekayupak, Yosita Panraksa, Jutiporn Yukird, Orawon Chailapakul, Nipapan Ruecha
Summary: This article comprehensively reviews the fabrication methods and device designs of microfluidic paper-based analytical devices (mu PADs), focusing on their applications in motif identification. The advantages and disadvantages of different techniques and designs are compared, and various powerful detection methods are integrated into mu PADs for medicinal diagnosis. A great prototype for real-life application breakthrough is also discussed.
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Yunfeng Zai, Zunliang Wang, Yongjun Ding, Chao Min, Yuan Liu, Huan Zhao, Enben Su, Nongyue He
Summary: Molecular point-of-care testing (POCT) system is crucial for the prevention and control of infectious diseases. We propose a gravity-driven microfluidic cartridge for molecular POCT detection, which achieves precise control of flow rates and enhances pumping efficiency. Computational fluid dynamics simulations demonstrate the effectiveness of our optimized design, and the accurate detection of human papillomavirus type 16 virus in clinical samples validates the performance of the optimized cartridge for POCT detection.
Article
Engineering, Environmental
Seong-Geun Jeong, Dong-Ho Kim, Jingyeong Kim, Ji-Hyun Kim, Sanggeun Song, Chang-Soo Lee
Summary: This study presents a method to achieve automatic multistep digital analysis in a single-sheet 3D paper-based microfluidic analytical device (3D-mu PAD) by controlling the penetration of different wax inks into paper. The methodology allows for sequential fluid delivery and multistep biochemical reactions, which can be integrated with digital assays without the need for external instruments. The potential commercial application of this approach is significant.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Energy & Fuels
Mosfera A. Chowdury, Farrukh Khalid
Summary: Due to the lack of affordable testing infrastructure, low- and medium-income countries are more affected by COVID-19 economically and socially. A proposed COVID-19 Point-of-Care (POC) ecosystem model aims to facilitate technological development and utilize locally available components. Collaboration among academia, government bodies, and entrepreneurs is crucial, and a microfluidic paper-based analytical POC platform for detecting COVID-19 analyte with patient-derived saliva is proposed in the study.
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2021)
Article
Biochemical Research Methods
Gayan C. Bandara, Linus J. Unitan, Matthew H. Kremer, Owen T. Shellhammer, Shay Bracha, Vincent T. Remcho
Summary: Traditional blood separation methods require complex equipment and large sample volumes, but with the development of microfluidic devices, blood separation and analysis can now be achieved effectively in small sample sizes. By utilizing a simple fabrication process, these devices can separate blood and integrate colorimetric assay chemistries for semi-quantitative detection in microscale samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Hanbi Kim, Hyeonseek Park, Doo Ryeon Chung, Taekyung Kim, Eunkyoung Park, Minhee Kang
Summary: We have developed a portable and user-friendly blood plasma-separation device that can be used with rapid diagnostic test kits. This device allows for quick separation of plasma samples and enables blood testing through connected assay kits.
Article
Biochemistry & Molecular Biology
Mahendra Kumar Prajapat, Kirti Jain, Debika Choudhury, Nikhil Raj, Supreet Saini
MOLECULAR BIOSYSTEMS
(2016)
Article
Biochemistry & Molecular Biology
R. G. Brajesh, Nikhil Raj, Supreet Saini
MOLECULAR BIOSYSTEMS
(2017)
Article
Nanoscience & Nanotechnology
N. Raj, V. Breedveld, D. W. Hess
Summary: Microfluidic analytical devices on paper have great potential for disease diagnostics in resource-limited areas, but current commercialization is hindered by technical challenges. The separation of plasma from whole blood is a key challenge that needs to be addressed.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Article
Nanoscience & Nanotechnology
Nicole E. Pollok, Yi Peng, Nikhil Raj, Charuksha Walgama, Richard M. Crooks
Summary: This paper demonstrates the use of dual-shaped silver nanoparticles as detection labels for electrochemical bioassays, showing that using both shapes simultaneously can lower the limit of detection compared to using either shape independently. By optimizing the ratio of the two types of AgNP labels, it is possible to decrease the LOD of the assays without compromising the dynamic range, achieving the clinically relevant range for heart failure risk stratification.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Analytical
Nikhil Raj, Richard M. Crooks
Summary: In this article, the application of linear sweep anodic stripping voltammetry (LASV) and square-wave anodic stripping voltammetry (SWASV) for detection of a nano metalloimmunoassay is compared. The results show that SWASV can significantly improve the detection efficiency and demonstrate better performance in terms of calibration sensitivity and limit of detection.
Article
Chemistry, Analytical
Nikhil Raj, Richard M. Crooks
Summary: An easily fabricated plastic-based lateral flow device, called ocFlow, is reported for metalloimmunoassays. The device is capable of detecting two types of immunoconjugates, with low detection limits.
Article
Biochemical Research Methods
N. Raj, V. Breedveld, D. W. Hess
Meeting Abstract
Biochemistry & Molecular Biology
S. Saini, K. Jain, M. K. Prajapat, D. Choudhury
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)